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Protein

Brefeldin A-inhibited guanine nucleotide-exchange protein 1

Gene

ARFGEF1

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Promotes guanine-nucleotide exchange on ARF1 and ARF3. Promotes the activation of ARF1/ARF3 through replacement of GDP with GTP. Involved in vesicular trafficking. Required for the maintenance of Golgi structure; the function may be independent of its GEF activity. Required for the maturaion of integrin beta-1 in the Golgi. Involved in the establishment and persistence of cell polarity during directed cell movement in wound healing. Proposed to act as A kinase-anchoring protein (AKAP) and may mediate crosstalk between Arf and PKA pathways. Inhibits GAP activity of MYO9B probably through competetive RhoA binding. The function in the nucleus remains to be determined (By similarity).By similarity

Enzyme regulationi

Inhibited by brefeldin A.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Guanine-nucleotide releasing factor

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Brefeldin A-inhibited guanine nucleotide-exchange protein 1
Short name:
Brefeldin A-inhibited GEP 1
Alternative name(s):
ADP-ribosylation factor guanine nucleotide-exchange factor 1
p200 ARF guanine nucleotide exchange factor
p200 ARF-GEP1
Gene namesi
Name:ARFGEF1
Synonyms:ARFGEP1, BIG1
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Unplaced

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Golgi apparatus, Membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 18491849Brefeldin A-inhibited guanine nucleotide-exchange protein 1PRO_0000120206Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei234 – 2341PhosphoserineBy similarity
Modified residuei243 – 2431PhosphoserineBy similarity
Modified residuei286 – 2861PhosphoserineBy similarity
Modified residuei289 – 2891PhosphoserineBy similarity
Modified residuei290 – 2901PhosphoserineBy similarity
Modified residuei396 – 3961PhosphoserineBy similarity
Modified residuei397 – 3971PhosphoserineBy similarity
Modified residuei664 – 6641PhosphoserineBy similarity
Modified residuei667 – 6671PhosphoserineBy similarity
Modified residuei681 – 6811PhosphothreonineBy similarity
Modified residuei1079 – 10791PhosphoserineBy similarity
Modified residuei1569 – 15691PhosphoserineBy similarity
Modified residuei1593 – 15931PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated. In vitro phosphorylated by PKA reducing its GEF activity and dephosphorylated by phosphatase PP1 (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO46382.
PRIDEiO46382.

Expressioni

Tissue specificityi

Abundantly expressed in kidney, somewhat less abundant in lung, spleen, and brain, and still less abundant in heart.

Interactioni

Subunit structurei

Homodimer. Interacts with ARFGEF2/BIG2; both proteins are probably part of the same or very similar macromolecular complexes. Interacts with FKBP2. Interacts with MYO9B. Interacts with PRKAR1A and PRKAR2A. Interacts with PPP1CC. Interacts with NCL, FBL, NUP62 and U3 small nucleolar RNA. Interacts with DPY30. Interacts with PDE3A. Interacts with KANK1. Interacts with TBC1D22A and TBC1D22B.By similarity

GO - Molecular functioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019553.

Structurei

3D structure databases

ProteinModelPortaliO46382.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini709 – 840132SEC7PROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 224223DCB; DCB:DCB and DCB:HUS domain interactionBy similarityAdd
BLAST
Regioni557 – 57721HUS; DCB:HUS domain interactionBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi711 – 7155Nuclear localization signal (NLS)By similarity

Sequence similaritiesi

Contains 1 SEC7 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
HOVERGENiHBG004846.
InParanoidiO46382.
KOiK18442.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O46382-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYEGKKTKNM FLTRALEKIL ADKEVKKAHH SQLRKACEVA LEEIKAETEK
60 70 80 90 100
QSPPHGEAKA GSSTLPPVKS KTNFIEADKY FLPFELACQS KCPRIVSTSL
110 120 130 140 150
DCLQKLIAYG HLTGNAPDST TPGKKLIDRI IETICGCFQG PQTDEGVQLQ
160 170 180 190 200
IIKALLTAVT SQHIEIHEGT VLQAVRTCYN IYLASKNLIN QTTAKATLTQ
210 220 230 240 250
MLNVIFARME NQALQEAKQM EKERHRQHHH LLQSPVSHHE PESPQLRYLP
260 270 280 290 300
PQTVDHIPQE HEGDLDPQTN DVDKSLQDDT EPENGSDISS AENEQTEADQ
310 320 330 340 350
ATAAETLSKN DILYDGENHD CEEKPQDIVQ SIVEEMVNIV VGDTGERTTI
360 370 380 390 400
NVSADGNNGT IEDGSDSENI QANGIPGTPI SVAYTPSLPD DRLSVSSNDT
410 420 430 440 450
QESGNSSGPS PGAKFSHILQ KDAFLVFRSL CKLSMKPLSD GPPDPKSHEL
460 470 480 490 500
RSKILSLQLL LSILQNAGPI FGTNEMFINA IKQYLCVALS KNGVSSVPEV
510 520 530 540 550
FELSLSIFLT LLSNFKTHLK MQIEVFFKEI FLYILETSTS SFDHKWMVIQ
560 570 580 590 600
TLTRICADAQ SVVDIYVNYD CDLNAANIFE RLVNDLSKIA QGRGSQELGM
610 620 630 640 650
SNVQELSLRK KGLECLVSIL KCMVEWSKDQ YVNPNSQTTL GQEKPSEQET
660 670 680 690 700
SEMKHPETIN RYGSLNSLES TSSSGIGSYS TQMSGTDNPE QFEVLKQQKE
710 720 730 740 750
IIEQGIDLFT KKPKRGIQYL QEQGMLGTTP EDIAQFLHQE ERLDSTQVGE
760 770 780 790 800
FLGDNDKFNK EVMYAYVDQH DFSGKDFVSA LRMFLEGFRL PGEAQKIDRL
810 820 830 840 850
MEKFAARYLE CNQGQTLFAS ADTAYVLAYS IIMLTTDLHS PQVKNKMTKE
860 870 880 890 900
QYIKMNRGIN DSKDLPEEYL SAIYNEIAGK KISMKETKEL TIPAKSSKQN
910 920 930 940 950
VASEKQRRLL YNLEMEQMAK TAKALMEAVS HVQAPFTSAT HLEHVRPMFK
960 970 980 990 1000
LAWTPFLAAF SVGLQDCDDT EVASLCLEGI RCAIRIACIF SIQLERDAYV
1010 1020 1030 1040 1050
QALARFTLLT VSSGITEMKQ KNIDTIKTLI TVAHTDGNYL GNSWHEILKC
1060 1070 1080 1090 1100
ISQLELAQLI GTGVKPRYIS GTVRGREGSL TGAKDQAPDE FVGLGLVGGN
1110 1120 1130 1140 1150
VDWKQIASIQ ESIGETSSQS VVVAVDRIFT GSTRLDGNAI VDFVRWLCAV
1160 1170 1180 1190 1200
SMDELLSTTH PRMFSLQKIV EISYYNMGRI RLQWSRIWEV IGDHFNKVGC
1210 1220 1230 1240 1250
NPNEDVAIFA VDSLRQLSMK FLEKGELANF RFQKDFLRPF EHIMKRNRSP
1260 1270 1280 1290 1300
TIRDMVVRCI AQMVNSQAAN IRSGWKNIFS VFHLAASDQD ESIVELAFQT
1310 1320 1330 1340 1350
TGHIVTLVFE KHFPATIDSF QDAVKCLSEF ACNAAFPDTS MEAIRLIRHC
1360 1370 1380 1390 1400
AKYVSDRPQA FKEYTSDDMN VAPEDRVWVR GWFPILFELS CIINRCKLDV
1410 1420 1430 1440 1450
RTRGLTVMFE IMKTYGYTYE KHWWQDLFRI VFRIFDNMKL PEQQTEKAEW
1460 1470 1480 1490 1500
MTTTCNHALY AICDVFTQYL EVLSDVLLDD IFAQLYWCVQ QDNEQLARSG
1510 1520 1530 1540 1550
TNCLENVVIL NGEKFTLEIW DKTCNCTLDI FKTTIPHALL TWRPISGETA
1560 1570 1580 1590 1600
PPTPSPVSEN QLDTISQKSV DIHDSIQPRS ADNRQQAPLA SVSTVNEEIS
1610 1620 1630 1640 1650
KIKPTAKFPE QKLFAALLIK CVVQLELIQT IDNIVFFPAT SRKEDAENLA
1660 1670 1680 1690 1700
AAQRDAVDFD VRVDTQDQGM YRFLTSQQLF KLLDCLLESH RFAKAFNSNN
1710 1720 1730 1740 1750
EQRTALWKAG FKGKSKPNLL KQETSSLACG LRILFRMYTD ESRASAWEEV
1760 1770 1780 1790 1800
QQRLLNVCSE ALSYFLTLTS ESHREAWTNL LLLFLTKVLK ISDNRFKAHA
1810 1820 1830 1840
SFYYPLLCEI MQFDLIPELR AVLRRFFLRI GVVFQISQPP EQELGINKQ
Length:1,849
Mass (Da):208,712
Last modified:June 1, 1998 - v1
Checksum:i8A8A9C864B899E7D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023451 mRNA. Translation: AAC48782.1.
PIRiT14096.
RefSeqiNP_776422.1. NM_173997.2.
UniGeneiBt.4549.

Genome annotation databases

GeneIDi281022.
KEGGibta:281022.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF023451 mRNA. Translation: AAC48782.1.
PIRiT14096.
RefSeqiNP_776422.1. NM_173997.2.
UniGeneiBt.4549.

3D structure databases

ProteinModelPortaliO46382.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000019553.

Proteomic databases

PaxDbiO46382.
PRIDEiO46382.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi281022.
KEGGibta:281022.

Organism-specific databases

CTDi10565.

Phylogenomic databases

eggNOGiKOG0929. Eukaryota.
COG5307. LUCA.
HOVERGENiHBG004846.
InParanoidiO46382.
KOiK18442.

Family and domain databases

Gene3Di1.10.1000.11. 1 hit.
1.25.10.10. 4 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032629. DCB_dom.
IPR023394. Sec7_alpha_orthog.
IPR015403. Sec7_C.
IPR000904. Sec7_dom.
IPR032691. Sec7_N.
[Graphical view]
PfamiPF16213. DCB. 1 hit.
PF09324. DUF1981. 1 hit.
PF01369. Sec7. 1 hit.
PF12783. Sec7_N. 1 hit.
[Graphical view]
SMARTiSM00222. Sec7. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 3 hits.
SSF48425. SSF48425. 1 hit.
PROSITEiPS50190. SEC7. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor."
    Morinaga N., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 94:12926-12931(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Brain.
  2. "Isolation of a brefeldin A-inhibited guanine nucleotide-exchange protein for ADP ribosylation factor (ARF) 1 and ARF3 that contains a Sec7-like domain."
    Morinaga N., Tsai S.-C., Moss J., Vaughan M.
    Proc. Natl. Acad. Sci. U.S.A. 93:12856-12860(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PARTIAL PROTEIN SEQUENCE, CHARACTERIZATION.
    Tissue: Brain.

Entry informationi

Entry nameiBIG1_BOVIN
AccessioniPrimary (citable) accession number: O46382
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.