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O46310

- RIR2_LEIAM

UniProt

O46310 - RIR2_LEIAM

Protein

Ribonucleoside-diphosphate reductase small subunit

Gene
N/A
Organism
Leishmania amazonensis
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 75 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    • Comment

    Functioni

    Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.

    Catalytic activityi

    2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.PROSITE-ProRule annotation

    Cofactori

    Binds 2 iron ions per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi93 – 931Iron 1PROSITE-ProRule annotation
    Metal bindingi124 – 1241Iron 1PROSITE-ProRule annotation
    Metal bindingi124 – 1241Iron 2By similarity
    Metal bindingi127 – 1271Iron 1PROSITE-ProRule annotation
    Active sitei131 – 1311PROSITE-ProRule annotation
    Metal bindingi186 – 1861Iron 2By similarity
    Metal bindingi220 – 2201Iron 2By similarity
    Metal bindingi223 – 2231Iron 2By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Source: UniProtKB-EC

    GO - Biological processi

    1. deoxyribonucleoside diphosphate metabolic process Source: InterPro
    2. DNA replication Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    DNA replication

    Keywords - Ligandi

    Iron, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00326.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribonucleoside-diphosphate reductase small subunit (EC:1.17.4.1)
    Alternative name(s):
    Ribonucleotide reductase M2 subunit
    Ribonucleotide reductase small subunit
    OrganismiLeishmania amazonensis
    Taxonomic identifieri5659 [NCBI]
    Taxonomic lineageiEukaryotaEuglenozoaKinetoplastidaTrypanosomatidaeLeishmaniinaeLeishmania

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 345345Ribonucleoside-diphosphate reductase small subunitPRO_0000190457Add
    BLAST

    Interactioni

    Subunit structurei

    Heterodimer of a large and a small subunit.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliO46310.
    SMRiO46310. Positions 21-306.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Family and domain databases

    Gene3Di1.10.620.20. 1 hit.
    InterProiIPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view]
    PANTHERiPTHR23409. PTHR23409. 1 hit.
    PfamiPF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view]
    SUPFAMiSSF47240. SSF47240. 1 hit.
    PROSITEiPS00368. RIBORED_SMALL. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O46310-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKPVAAGAEV LPADKVAQGT NAEEEPLQQE NPFRYVLFPI QYHDIWRKYK    50
    EQESCIWTVE EIDLGNDMKD WATLNDGERH FIKHVLAFFA GSDGIVIENL 100
    AQRFMSDVKV PEARAFYGFQ LMMENIHSET YSVLLDTYIT DSEEKLRLLH 150
    AIQTIPCIQK KAEWPVRWIG SSASFQQRLI GFAAVEGIFF SGSFCALFWL 200
    KKRGLMPGLT FSNELISRDE GLHTDFACLL YNSHIKHKLP RERVLEIIVD 250
    AVNIEREFIC DALPVRLIGM NAELMAQYIE FVADRLLVSL GEEKHYRSTQ 300
    PFDFMEMISL QGKTNFFEKR VGEYQKAGVM STEGTSKKFS LSEDF 345
    Length:345
    Mass (Da):39,651
    Last modified:June 1, 1998 - v1
    Checksum:iD0829B1BFE9EA115
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005247 Genomic DNA. Translation: AAC08302.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF005247 Genomic DNA. Translation: AAC08302.1 .

    3D structure databases

    ProteinModelPortali O46310.
    SMRi O46310. Positions 21-306.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00326 .

    Family and domain databases

    Gene3Di 1.10.620.20. 1 hit.
    InterProi IPR009078. Ferritin-like_SF.
    IPR012348. RNR-rel.
    IPR000358. RNR_small.
    [Graphical view ]
    PANTHERi PTHR23409. PTHR23409. 1 hit.
    Pfami PF00268. Ribonuc_red_sm. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47240. SSF47240. 1 hit.
    PROSITEi PS00368. RIBORED_SMALL. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and functional analysis of the ribonucleotide reductase gene small subunit from hydroxyurea-resistant Leishmania mexicana amazonensis."
      Lye L.-F., Hsieh Y.-H., Su K.-E., Lee S.T.
      Mol. Biochem. Parasitol. 90:353-358(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

    Entry informationi

    Entry nameiRIR2_LEIAM
    AccessioniPrimary (citable) accession number: O46310
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 75 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3