O46310 (RIR2_LEIAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Ribonucleoside-diphosphate reductase small subunit EC=1.17.4.1 Alternative name(s): Ribonucleotide reductase M2 subunit Ribonucleotide reductase small subunit
Organism	Leishmania amazonensis
Taxonomic identifier	5659 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Leishmaniinae › Leishmania › Leishmania › Leishmania mexicana species complex

Protein attributes

Sequence length	345 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides.
Catalytic activity	2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H2O = ribonucleoside diphosphate + thioredoxin.
Cofactor	Binds 2 iron ions per subunit By similarity.
Pathway	Genetic information processing; DNA replication.
Subunit structure	Heterodimer of a large and a small subunit By similarity.
Sequence similarities	Belongs to the ribonucleoside diphosphate reductase small chain family.

Ontologies

Keywords
Biological process	DNA replication
Ligand	Iron Metal-binding
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-UniPathway deoxyribonucleoside diphosphate metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	ribonucleoside-diphosphate reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor Inferred from electronic annotation. Source: EC transition metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 345	345	Ribonucleoside-diphosphate reductase small subunit		PRO_0000190457
Sites
	Active site	131	1	By similarity
	Metal binding	93	1	Iron 1 By similarity
	Metal binding	124	1	Iron 1 By similarity
	Metal binding	124	1	Iron 2 By similarity
	Metal binding	127	1	Iron 1 By similarity
	Metal binding	186	1	Iron 2 By similarity
	Metal binding	220	1	Iron 2 By similarity
	Metal binding	223	1	Iron 2 By similarity

Sequences

	Sequence		Length	Mass (Da)
	O46310 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: D0829B1BFE9EA115	FASTA	345	39,651
	10 20 30 40 50 60 MKPVAAGAEV LPADKVAQGT NAEEEPLQQE NPFRYVLFPI QYHDIWRKYK EQESCIWTVE 70 80 90 100 110 120 EIDLGNDMKD WATLNDGERH FIKHVLAFFA GSDGIVIENL AQRFMSDVKV PEARAFYGFQ 130 140 150 160 170 180 LMMENIHSET YSVLLDTYIT DSEEKLRLLH AIQTIPCIQK KAEWPVRWIG SSASFQQRLI 190 200 210 220 230 240 GFAAVEGIFF SGSFCALFWL KKRGLMPGLT FSNELISRDE GLHTDFACLL YNSHIKHKLP 250 260 270 280 290 300 RERVLEIIVD AVNIEREFIC DALPVRLIGM NAELMAQYIE FVADRLLVSL GEEKHYRSTQ 310 320 330 340 PFDFMEMISL QGKTNFFEKR VGEYQKAGVM STEGTSKKFS LSEDF

References

[1]

"Cloning and functional analysis of the ribonucleotide reductase gene small subunit from hydroxyurea-resistant Leishmania mexicana amazonensis." Lye L.-F., Hsieh Y.-H., Su K.-E., Lee S.T. Mol. Biochem. Parasitol. 90:353-358(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF005247 Genomic DNA. Translation: AAC08302.1.
3D structure databases
ProteinModelPortal	O46310.
SMR	O46310. Positions 21-306.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00326.
Family and domain databases
Gene3D	1.10.620.20. 1 hit.
InterPro	IPR009078. Ferritin-like_SF. IPR012348. RNR-rel. IPR000358. RNR_small. [Graphical view]
PANTHER	PTHR23409. PTHR23409. 1 hit.
Pfam	PF00268. Ribonuc_red_sm. 1 hit. [Graphical view]
SUPFAM	SSF47240. Ferritin/RR_like. 1 hit.
PROSITE	PS00368. RIBORED_SMALL. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RIR2_LEIAM

Accession

Primary (citable) accession number: O46310

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)