O46203 (A32CD_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Accessory gland protein Acp32CD

Gene names

Name:	Acp32CD
ORF Names:	CG4605

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Responsible for physiological and behavioral changes in mated female flies. Ref.1
Subcellular location	Secreted Probable.
Tissue specificity	Seminal fluid. Ref.1
Caution	In strain Berkeley it could be the product of a pseudogene.
Sequence caution	The sequence AAF53055.2 differs from that shown. Reason: Frameshift at several positions. The sequence AAL89983.1 differs from that shown. Reason: Frameshift at several positions.

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Coding sequence diversity	Alternative initiation Polymorphism
Domain	Signal
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	negative regulation of female receptivity, post-mating Non-traceable author statement Ref.1. Source: UniProtKB
Cellular component	extracellular region Traceable author statement Ref.1. Source: UniProtKB
Molecular function	hormone activity Non-traceable author statement Ref.1. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform Long (identifier: O46203-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Short (identifier: O46203-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 329	308	Accessory gland protein Acp32CD		PRO_0000020580
Regions
	Compositional bias	100 – 182	83	Gly-rich
	Compositional bias	200 – 326	127	Arg-rich
Natural variations
	Alternative sequence	1 – 88	88	Missing in isoform Short.		VSP_018775
	Natural variant	45	1	D → E in strain: WS1, WS9, WS16, Zim5, Zim10, Zim24, Zim30, Zim32 and Zim35. Ref.5
	Natural variant	52	1	A → D in strain: 7, WS1, WS12, WS16, WS17, Zim10, Zim22, Zim32 and Zim35. Ref.5
	Natural variant	63	1	A → T in strain: 7, WS1, WS9, WS16, WS17 and Zim10. Ref.5
	Natural variant	69	1	L → P in strain: WS9 and WS19. Ref.5
	Natural variant	273 – 275	3	TRM → PRT in strain: WS9, Zim30 and Zim22. Ref.5
	Natural variant	275	1	M → T in strain: WS12, WS17 and WS26. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	Isoform Long [UniParc]. Last modified June 1, 2001. Version 2. Checksum: 5655CCA73759CFEA	FASTA	329	35,557
	10 20 30 40 50 60 MPPLLRHCFG HAFIGLPLFN GQEQPRPQSN RFDSGQRRSS LYIRDGRTAR AAQRCSDVAD 70 80 90 100 110 120 ADAATHWLLG PVALGQLPEH GALGQKYYMN FAFNNNNPDG EGGTGVDGGG GGAGGGAAGP 130 140 150 160 170 180 GGGTGDSPHS QEGDGSAATD NPNDDHATSA DNSLATDGDA IGKKESGGGS DGKSDSKDSS 190 200 210 220 230 240 GGNDATPANG HDDDNDDSDR RMPRIDKIRK RRPDRRGSAP ITAITVATRS DRRQLWRAVR 250 260 270 280 290 300 AVHLREQREP RTFGSNAGSN QRTMEPVRAV RRTRMPRKWP AKRLLNGQAT CQMDPKLEPR 310 320 KMTTRRCNTT GRSDHRFARG TLEERRHFN
	Isoform Short [UniParc]. Checksum: ADAD277E7384F94F	FASTA	241	25,816
	10 20 30 40 50 60 MNFAFNNNNP DGEGGTGVDG GGGGAGGGAA GPGGGTGDSP HSQEGDGSAA TDNPNDDHAT 70 80 90 100 110 120 SADNSLATDG DAIGKKESGG GSDGKSDSKD SSGGNDATPA NGHDDDNDDS DRRMPRIDKI 130 140 150 160 170 180 RKRRPDRRGS APITAITVAT RSDRRQLWRA VRAVHLREQR EPRTFGSNAG SNQRTMEPVR 190 200 210 220 230 240 AVRRTRMPRK WPAKRLLNGQ ATCQMDPKLE PRKMTTRRCN TTGRSDHRFA RGTLEERRHF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes for male accessory gland proteins in Drosophila melanogaster." Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W., Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K. Insect Biochem. Mol. Biol. 27:825-834(1997) [PubMed: 9474779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Male accessory gland.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
[5]	"Molecular population genetics of male accessory gland proteins in Drosophila." Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G. Genetics 156:1879-1888(2000) [PubMed: 11102381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 24-289. Strain: 7, WS1, WS12, WS16, WS17, WS19, WS26, WS47, WS9, Zim10, Zim22, Zim24, Zim30, Zim32, Zim35 and Zim5.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U85764 Genomic DNA. Translation: AAB96388.1. U85764 Genomic DNA. Translation: AAB96389.1. U90948 mRNA. Translation: AAB96395.1. AE014134 Genomic DNA. Translation: AAF53055.2. Frameshift. AY089245 mRNA. Translation: AAL89983.1. Frameshift. AY010544 Genomic DNA. Translation: AAG32576.1. AY010545 Genomic DNA. Translation: AAG32577.2. AY010546 Genomic DNA. Translation: AAG32578.1. AY010547 Genomic DNA. Translation: AAG32579.1. AY010548 Genomic DNA. Translation: AAG32580.1. AY010549 Genomic DNA. Translation: AAG32581.2. AY010550 Genomic DNA. Translation: AAG32582.1. AY010551 Genomic DNA. Translation: AAG32583.1. AY010552 Genomic DNA. Translation: AAG32584.1. AY010553 Genomic DNA. Translation: AAG32585.1. AY010554 Genomic DNA. Translation: AAG32586.1. AY010555 Genomic DNA. Translation: AAG32587.1. AY010556 Genomic DNA. Translation: AAG32588.1. AY010557 Genomic DNA. Translation: AAG32589.1. AY010558 Genomic DNA. Translation: AAG32590.1. AY010559 Genomic DNA. Translation: AAG32591.1.
RefSeq	NP_477328.2. NM_057980.3.
UniGene	Dm.4870.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O46203.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	34531.
KEGG	dme:Dmel_CG4605.
Organism-specific databases
CTD	34531.
FlyBase	FBgn0023415. Acp32CD.
Phylogenomic databases
InParanoid	O46203.
OrthoDB	EOG4RN8RV.
Gene expression databases
Bgee	O46203.
Family and domain databases
ProtoNet	Search...
Other
NextBio	788930.

Entry information

Entry name

A32CD_DROME

Accession

Primary (citable) accession number: O46203
Secondary accession number(s): O46225 , Q8T4D3, Q9TY45, Q9VKL2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 15, 2002
Last sequence update:	June 1, 2001
Last modified:	January 25, 2012
This is version 71 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project