Reviewed, UniProtKB/Swiss-Prot O46199 (A53E_DROME)

Names and origin

Protein names

Recommended name:
    Accessory gland protein Acp53Ea

Gene names

Name:	Acp53Ea
ORF Names:	CG8622

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	120 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for physiological and behavioral changes in mated female flies. Ref.1
Subcellular location	Secreted Probable.
Tissue specificity	Main cells of accessory gland and seminal fluid. Ref.1
Sequence caution	The sequence AAM29231.1 differs from that shown. Reason: Erroneous translation. Wrong choice of frame. The sequence AAM29231.1 differs from that shown. Reason: Frameshift at position 10.

Ontologies

Keywords
Biological process	Behavior
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	behavior Inferred from electronic annotation. Source: UniProtKB-KW sperm competition Traceable author statement. Source: FlyBase
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CG17292	Q9VLN2	1	EBI-142141,EBI-106635

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 120	97	Accessory gland protein Acp53Ea		PRO_0000020584
Natural variations
	Natural variant	7	1	T → A in strain: ZIM56.
	Natural variant	14	1	A → D in strain: M47.
	Natural variant	21	1	T → L in strain: M47.
	Natural variant	22	1	E → K in strain: ZIM26 and ZIM42.
	Natural variant	27	1	I → V in strain: ZIM56.
	Natural variant	70	1	N → K in strain: M09 and WS9.
	Natural variant	72	1	Y → C in strain: SFS 3.1.
	Natural variant	83	1	V → D in strain: ZIM30.
	Natural variant	108	1	Y → C in strain: ZIM2.

Sequences

	Sequence		Length	Mass (Da)
	O46199-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C576B846B4DC2871	FASTA	120	13,607
	10 20 30 40 50 60 MKLIKVTLVF SLLALVFVAQ TEAQNPIWEN WLACNRIGTK ALASLLRETI PTVRNLLNCI 70 80 90 100 110 120 DFNPPTDIGN SYLSKLKLYY ELVKRGALDK TQCLIVPLKE SVRLLRPYVK SLETNKCLGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"New genes for male accessory gland proteins in Drosophila melanogaster." Wolfner M.F., Harada H.A., Bertram M.J., Stelick T.J., Kraus K.W., Kalb J.M., Lung Y.O., Neubaum D.M., Park M., Tram U.K. Insect Biochem. Mol. Biol. 27:825-834(1997) [PubMed: 9474779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Canton-S. Tissue: Male accessory gland.
[2]	"Molecular population genetics of male accessory gland proteins in Drosophila." Begun D.J., Whitley P., Todd B.L., Waldrip-Dail H.M., Clark A.G. Genetics 156:1879-1888(2000) [PubMed: 11102381] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: WS1, WS12, WS16, WS19, WS26, WS47, WS49, WS56, WS6, WS9, ZIM2, ZIM26, ZIM30, ZIM42 and ZIM56.
[3]	"Population genetics of accessory gland proteins and sexual behavior in Drosophila melanogaster populations from Evolution Canyon." Panhuis T.M., Swanson W.J., Nunney L. Evolution 57:2785-2791(2003) [PubMed: 14761057] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: NFS 5.1, NFS 5.2, NFS 5.3, NFS 5.4, NFS 6.1, NFS 6.2, NFS 6.3, NFS 6.4, NFS 7.8, SFS 1.1, SFS 1.2, SFS 1.3, SFS 1.4, SFS 2.2, SFS 2.3, SFS 2.4, SFS 3.1, SFS 3.2, SFS 3.3 and SFS 3.4.
[4]	"Molecular evolution and population genetics of duplicated accessory gland protein genes in Drosophila." Holloway A.K., Begun D.J. Mol. Biol. Evol. 21:1625-1628(2004) [PubMed: 15215320] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: M01, M06, M09, M16, M26 and M47.
[5]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[6]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
[7]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U85760 mRNA. Translation: AAB96384.1. AY010593 Genomic DNA. Translation: AAG37375.1. AY010594 Genomic DNA. Translation: AAG37376.1. AY010595 Genomic DNA. Translation: AAG37377.1. AY010596 Genomic DNA. Translation: AAG37378.1. AY010597 Genomic DNA. Translation: AAG37379.1. AY010598 Genomic DNA. Translation: AAG37380.1. AY010599 Genomic DNA. Translation: AAG37381.1. AY010600 Genomic DNA. Translation: AAG37382.1. AY010601 Genomic DNA. Translation: AAG37383.1. AY010602 Genomic DNA. Translation: AAG37384.1. AY010603 Genomic DNA. Translation: AAG37385.1. AY010604 Genomic DNA. Translation: AAG37386.1. AY010605 Genomic DNA. Translation: AAG37387.1. AY010606 Genomic DNA. Translation: AAG37388.1. AY010607 Genomic DNA. Translation: AAG37389.1. AY344265 Genomic DNA. Translation: AAR04580.1. AY344266 Genomic DNA. Translation: AAR04581.1. AY344267 Genomic DNA. Translation: AAR04582.1. AY344268 Genomic DNA. Translation: AAR04583.1. AY344269 Genomic DNA. Translation: AAR04584.1. AY344270 Genomic DNA. Translation: AAR04585.1. AY344271 Genomic DNA. Translation: AAR04586.1. AY344272 Genomic DNA. Translation: AAR04587.1. AY344273 Genomic DNA. Translation: AAR04588.1. AY344274 Genomic DNA. Translation: AAR04589.1. AY344275 Genomic DNA. Translation: AAR04590.1. AY344276 Genomic DNA. Translation: AAR04591.1. AY344277 Genomic DNA. Translation: AAR04592.1. AY344278 Genomic DNA. Translation: AAR04593.1. AY344279 Genomic DNA. Translation: AAR04594.1. AY344280 Genomic DNA. Translation: AAR04595.1. AY344281 Genomic DNA. Translation: AAR04596.1. AY344282 Genomic DNA. Translation: AAR04597.1. AY344283 Genomic DNA. Translation: AAR04598.1. AY344284 Genomic DNA. Translation: AAR04599.1. AY635241 Genomic DNA. Translation: AAT49147.1. AY635242 Genomic DNA. Translation: AAT49148.1. AY635243 Genomic DNA. Translation: AAT49149.1. AY635244 Genomic DNA. Translation: AAT49150.1. AY635245 Genomic DNA. Translation: AAT49151.1. AY635246 Genomic DNA. Translation: AAT49152.1. AE013599 Genomic DNA. Translation: AAF57959.1. AY113226 mRNA. Translation: AAM29231.1. Frameshift.
RefSeq	NP_477151.1.
UniGene	Dm.19942
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:22625N.
IntAct	O46199. 2 interactions.
STRING	O46199.
Proteomic databases
PRIDE	O46199.
Genome annotation databases
Ensembl	FBtr0087080; FBpp0086228; FBgn0015584; Drosophila melanogaster. [Genome view]
GeneID	36874.
KEGG	dme:Dmel_CG8622.
Organism-specific databases
CTD	36874.
FlyBase	FBgn0015584. Acp53Ea.
Phylogenomic databases
OMA	LECTHIG
OrthoDB	EOG92Z4X3
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-005021-MON.
Gene expression databases
GermOnline	CG8622. Drosophila melanogaster.
Family and domain databases
InterPro	IPR009392. ACP53EA. [Graphical view]
PANTHER	PTHR12407. ACP53EA. 1 hit.
Pfam	PF06313. ACP53EA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	800822.

Entry information

Entry name

A53E_DROME

Accession

Primary (citable) accession number: O46199
Secondary accession number(s): Q6GUV6 , Q6VBF9, Q6VBG0, Q6VBG1, Q8MZD8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	June 1, 1998
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project