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O46162 (SGP1_SCHGR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Serine protease inhibitor I/II

Cleaved into the following 2 chains:

  1. Protease inhibitor SGPI-1
    Alternative name(s):
    Schistocerca gregaria trypsin inhibitor
    Short name=SGTI
  2. Protease inhibitor SGPI-2
    Alternative name(s):
    Schistocerca gregaria chymotrypsin inhibitor
    Short name=SGCI
OrganismSchistocerca gregaria (Desert locust)
Taxonomic identifier7010 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaOrthopteraCaeliferaAcridomorphaAcridoideaAcrididaeCyrtacanthacridinaeSchistocerca

Protein attributes

Sequence length92 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

In vitro, SGPI-1/SGCI is active against alpha-chymotrypsin and trypsin while SGPI-2/SGTI is active against alpha-chymotrypsin and pancreatic elastase. Ref.2

Subcellular location

Secreted.

Tissue specificity

Expressed in hemolymph, ovaries, testes and fat body of adults but are absent in the gut. Also present in larval hemolymph and fat body. Ref.2

Sequence similarities

Belongs to the protease inhibitor I19 family. [View classification]

Contains 2 pacifastin domains.

Ontologies

Keywords
   Cellular componentSecreted
   DomainRepeat
Signal
   Molecular functionProtease inhibitor
Serine protease inhibitor
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical term3D-structure
Direct protein sequencing
Gene Ontology (GO)
   Cellular_componentextracellular region

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionserine-type endopeptidase inhibitor activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 Ref.2
Peptide20 – 5435Protease inhibitor SGPI-1 Ref.2
PRO_0000026710
Peptide57 – 9135Protease inhibitor SGPI-2
PRO_0000026711

Regions

Domain20 – 5435Pacifastin 1
Domain57 – 9236Pacifastin 2

Sites

Site48 – 492Reactive bond
Site86 – 872Reactive bond

Amino acid modifications

Disulfide bond23 ↔ 38
Disulfide bond33 ↔ 51
Disulfide bond36 ↔ 46
Disulfide bond60 ↔ 75
Disulfide bond70 ↔ 89
Disulfide bond73 ↔ 84

Secondary structure

................ 92
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O46162 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 9E5A228C767C657C

FASTA929,842
        10         20         30         40         50         60 
MKLALALCAA FLLVVLVQAE QECTPGQTKK QDCNTCNCTP TGVWACTRKG CPPHKREVTC 

        70         80         90 
EPGTTFKDKC NTCRCGSDGK SAACTLKACP QK 

« Hide

References

[1]"Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression."
Vanden Broeck J., Chiou S.-J., Schoofs L., Hamdaoui A., Vandenbussche F., Simonet G., Wataleb S., De Loof A.
Eur. J. Biochem. 254:90-95(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Ovary.
[2]"Purification and characterization of a group of five novel peptide serine protease inhibitors from ovaries of the desert locust, Schistocerca gregaria."
Hamdaoui A., Wataleb S., Devreese B., Chiou S.-J., Vanden Broeck J., Van Beeumen J., De Loof A., Schoofs L.
FEBS Lett. 422:74-78(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 20-54 AND 57-91, FUNCTION, TISSUE SPECIFICITY.
Tissue: Ovary.
[3]"Structural and functional properties of a novel serine protease inhibiting peptide family in arthropods."
Simonet G., Claeys I., Vanden Broeck J.
Comp. Biochem. Physiol. 132B:247-255(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: STRUCTURE BY NMR OF 20-54 AND 57-91.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y09605 mRNA. Translation: CAA70818.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1KGMNMR-A57-91[»]
1KIONMR-A57-91[»]
1KJ0NMR-A20-54[»]
2F91X-ray1.20B20-54[»]
2XTTX-ray0.93A20-54[»]
3TVJX-ray1.28I57-85[»]
4DJZX-ray3.20H/I57-87[»]
ProteinModelPortalO46162.
SMRO46162. Positions 20-54, 57-91.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSI19.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR020862. Pacifastin_art.
IPR008037. Prot_inh_PMP.
IPR016307. Prtase-inh_pacifastin.
[Graphical view]
PfamPF05375. Pacifastin_I. 2 hits.
[Graphical view]
PIRSFPIRSF001625. Prot_inhib_pacifastin. 1 hit.
SUPFAMSSF57283. SSF57283. 2 hits.
PROSITEPS51446. PACIFASTIN. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceO46162.

Entry information

Entry nameSGP1_SCHGR
AccessionPrimary (citable) accession number: O46162
Entry history
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references