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Protein

Serine protease inhibitor I/II

Gene
N/A
Organism
Schistocerca gregaria (Desert locust)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In vitro, SGPI-1/SGCI is active against alpha-chymotrypsin and trypsin while SGPI-2/SGTI is active against alpha-chymotrypsin and pancreatic elastase.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei48 – 492Reactive bond
Sitei86 – 872Reactive bond

GO - Molecular functioni

  1. serine-type endopeptidase inhibitor activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI19.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor I/II
Cleaved into the following 2 chains:
Alternative name(s):
Schistocerca gregaria trypsin inhibitor
Short name:
SGTI
Alternative name(s):
Schistocerca gregaria chymotrypsin inhibitor
Short name:
SGCI
OrganismiSchistocerca gregaria (Desert locust)
Taxonomic identifieri7010 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraOrthopteroideaOrthopteraCaeliferaAcridomorphaAcridoideaAcrididaeCyrtacanthacridinaeSchistocerca

Subcellular locationi

GO - Cellular componenti

  1. extracellular region Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 19191 PublicationAdd
BLAST
Peptidei20 – 5435Protease inhibitor SGPI-1PRO_0000026710Add
BLAST
Peptidei57 – 9135Protease inhibitor SGPI-2PRO_0000026711Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi23 ↔ 38
Disulfide bondi33 ↔ 51
Disulfide bondi36 ↔ 46
Disulfide bondi60 ↔ 75
Disulfide bondi70 ↔ 89
Disulfide bondi73 ↔ 84

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Expressioni

Tissue specificityi

Expressed in hemolymph, ovaries, testes and fat body of adults but are absent in the gut. Also present in larval hemolymph and fat body.1 Publication

Structurei

Secondary structure

1
92
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi28 – 314Combined sources
Beta strandi34 – 385Combined sources
Beta strandi44 – 474Combined sources
Turni62 – 643Combined sources
Beta strandi65 – 684Combined sources
Beta strandi71 – 755Combined sources
Beta strandi77 – 804Combined sources
Beta strandi82 – 854Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KGMNMR-A57-91[»]
1KIONMR-A57-91[»]
1KJ0NMR-A20-54[»]
2F91X-ray1.20B20-54[»]
2XTTX-ray0.93A20-54[»]
3TVJX-ray1.28I57-91[»]
4DJZX-ray3.20H/I57-87[»]
ProteinModelPortaliO46162.
SMRiO46162. Positions 20-54, 57-91.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO46162.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini20 – 5435Pacifastin 1PROSITE-ProRule annotationAdd
BLAST
Domaini57 – 9236Pacifastin 2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protease inhibitor I19 family. [View classification]PROSITE-ProRule annotation
Contains 2 pacifastin domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Family and domain databases

InterProiIPR020862. Pacifastin_art.
IPR008037. Prot_inh_PMP.
IPR016307. Prtase-inh_pacifastin.
[Graphical view]
PfamiPF05375. Pacifastin_I. 2 hits.
[Graphical view]
PIRSFiPIRSF001625. Prot_inhib_pacifastin. 1 hit.
SUPFAMiSSF57283. SSF57283. 2 hits.
PROSITEiPS51446. PACIFASTIN. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O46162-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKLALALCAA FLLVVLVQAE QECTPGQTKK QDCNTCNCTP TGVWACTRKG
60 70 80 90
CPPHKREVTC EPGTTFKDKC NTCRCGSDGK SAACTLKACP QK
Length:92
Mass (Da):9,842
Last modified:June 1, 1998 - v1
Checksum:i9E5A228C767C657C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09605 mRNA. Translation: CAA70818.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y09605 mRNA. Translation: CAA70818.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1KGMNMR-A57-91[»]
1KIONMR-A57-91[»]
1KJ0NMR-A20-54[»]
2F91X-ray1.20B20-54[»]
2XTTX-ray0.93A20-54[»]
3TVJX-ray1.28I57-91[»]
4DJZX-ray3.20H/I57-87[»]
ProteinModelPortaliO46162.
SMRiO46162. Positions 20-54, 57-91.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiI19.001.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiO46162.

Family and domain databases

InterProiIPR020862. Pacifastin_art.
IPR008037. Prot_inh_PMP.
IPR016307. Prtase-inh_pacifastin.
[Graphical view]
PfamiPF05375. Pacifastin_I. 2 hits.
[Graphical view]
PIRSFiPIRSF001625. Prot_inhib_pacifastin. 1 hit.
SUPFAMiSSF57283. SSF57283. 2 hits.
PROSITEiPS51446. PACIFASTIN. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning of two cDNAs encoding three small serine protease inhibiting peptides from the desert locust Schistocerca gregaria and analysis of tissue-dependent and stage-dependent expression."
    Vanden Broeck J., Chiou S.-J., Schoofs L., Hamdaoui A., Vandenbussche F., Simonet G., Wataleb S., De Loof A.
    Eur. J. Biochem. 254:90-95(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Ovary.
  2. "Purification and characterization of a group of five novel peptide serine protease inhibitors from ovaries of the desert locust, Schistocerca gregaria."
    Hamdaoui A., Wataleb S., Devreese B., Chiou S.-J., Vanden Broeck J., Van Beeumen J., De Loof A., Schoofs L.
    FEBS Lett. 422:74-78(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 20-54 AND 57-91, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Ovary.
  3. "Structural and functional properties of a novel serine protease inhibiting peptide family in arthropods."
    Simonet G., Claeys I., Vanden Broeck J.
    Comp. Biochem. Physiol. 132B:247-255(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: STRUCTURE BY NMR OF 20-54 AND 57-91.

Entry informationi

Entry nameiSGP1_SCHGR
AccessioniPrimary (citable) accession number: O46162
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: June 1, 1998
Last modified: January 7, 2015
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.