Unreviewed, UniProtKB/TrEMBL O46111 (O46111_DROME)

Names and origin

Protein names

Recommended name:
    Ubiquitin-activating enzyme E1 RuleBase RU004072V0

Gene names

Name:	Uba1 FlyBase FBgn0023143
Synonyms:	UBA-1 EMBL CAA75816.1
ORF Names:	CG1782 FlyBase FBgn0023143

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome] EMBL CAA75816.1

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	1008 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Activates ubiquitin by first adenylating with ATP its carboxy-terminal glycine residue and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding an ubiquitin-E1 thioester and free AMP By similarity. RuleBase RU004072V0
Pathway	Protein modification; protein ubiquitination. RuleBase RU004072V0
Subunit structure	Monomer By similarity. RuleBase RU004072V0
Sequence similarities	Belongs to the ubiquitin-activating E1 family. RuleBase RU000519V1

Ontologies

Keywords
Biological process	Ubl conjugation pathway RuleBase RU000519V1
Ligand	ATP-binding RuleBase RU000519V1 Nucleotide-binding
Molecular function	Ligase RuleBase RU000519V1
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	neuron remodeling Inferred from mutant phenotype. Source: FlyBase protein modification process Inferred from electronic annotation. Source: InterPro
Cellular component	dendrite Inferred from mutant phenotype. Source: FlyBase
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro small protein activating enzyme activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	O46111-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 96A6E3AD53593F8B	FASTA	1,008	111,690
	10 20 30 40 50 60 MAGNSAAAGG DIDESLYSRQ LYVLGHDAMR RMANSDILLS GLGGLGLEIA KNVILGGVKS 70 80 90 100 110 120 ITLHDTATCG LHDLSSQFYL TEADIAKNRA EASCAQLAEL NNYVRTVSHT GPLTEEFLRK 130 140 150 160 170 180 FRVVVLTNSD GEEQQRIAKF GHENGIALII AETRGLFAKV FCDFGETFTI YDPDGTQPIS 190 200 210 220 230 240 TMIASITHDA QGVVTCLDET RHGFNDGDLL TFSEVQGMQE LNGCQPLKIT VLGPYTFSIG 250 260 270 280 290 300 DTSKFGEYMS GGVATQVKMP KTISFKPLAQ ATEEPEFLIS DFAKLDSPAT LHVAFNALSS 310 320 330 340 350 360 YRKAHNGALP RPWNEEDANS FLEVVRASSN AEVDEKLVLQ FAKICSGNTC PLDAAVGGIV 370 380 390 400 410 420 AQEVLKACSG KFTPIYQWLY FDALECLPTE GVEEADAQPV GSRYDSQIAI FGKKFQEKLA 430 440 450 460 470 480 DSKWFIVGAG AIGCELLKNF GMLGLGTGNG QIFVTDMDLI EKSNLNRQFL FRPHDVQKPK 490 500 510 520 530 540 SMTAADAIKR MNPEVNVTAY ELRVGAETEK VFSEDFFGKL DGVANALDNV DARIYMDRKC 550 560 570 580 590 600 IFNRIPLVET GTLGTLGNVQ VIVPFATESY SSSQDPPEKS IPICTLKNFP NAIEHTLQWA 610 620 630 640 650 660 RDAFEGVFKQ SAENAAQYIA DPQFTERIAK LPGIQPLEIL DSIKKALIDD KPKSFAHCVE 670 680 690 700 710 720 WARLYWEDQY VNQIKQLLFN FPPDQITSSG QPFWSGPKQC PDPLVFDVND PMHLDFIYAA 730 740 750 760 770 780 ANLRAEVYGI EQVRNRETIA ELVQKVKVPE FKPRSGVKIE TNEAAAAASA NNFDDGELDQ 790 800 810 820 830 840 DRVDKIISEL LKNADKSSKI TPLEFEKDDD SNLHMDFIVA CSNLRAANYK IPPADRHKSK 850 860 870 880 890 900 LIAGKIIPAI ATTTSVLSGL ACLEVIKLIV GHRDLVKFKK PCANLALPFM AFSEPLPAAK 910 920 930 940 950 960 NTYYGKEWTL WDRFEVTGEL SLQEFLNYFE ENEKLKITML SQGVSMLYSF FMPKAKCSER 970 980 990 1000 LPLPMSEVVR RVSKRRLEPH ERSLVFEICC NDVDGEDVEV PYVRYTLP

References

[1]	"The Drosophila ubiquitin activating enzyme." zur Lage P., Hartswood E., Bownes M., Finnegan D.J. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Canton-S EMBL CAA75816.1. Tissue: Brain EMBL CAA75816.1, Imaginal disc EMBL CAA75816.1 and Ovary EMBL CAA75816.1.
[2]	Zur Lage P.I. Submitted (DEC-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: Canton-S EMBL CAA75816.1. Tissue: Brain EMBL CAA75816.1, Imaginal disc EMBL CAA75816.1 and Ovary EMBL CAA75816.1.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y15895 mRNA. Translation: CAA75816.1.
3D structure databases
HSSP	HSSP built from PDB template 1JW9 based on UniProtKB P12282.
ModBase	Search...
Protein-protein interaction databases
IntAct	O46111. 3 interactions.
Proteomic databases
PRIDE	O46111.
Genome annotation databases
Ensembl	FBgn0023143. Drosophila melanogaster. [Contig view]
Organism-specific databases
FlyBase	FBgn0023143. Uba1.
Gene expression databases
ArrayExpress	O46111.
Family and domain databases
InterPro	IPR016040. NAD(P)-bd_dom. IPR000594. ThiF_NAD_FAD_bd. IPR018965. Ub-activating_enz_e1_C. IPR000127. UBact_repeat. IPR019572. Ubiquitin-activating_enzyme. IPR018075. UBQ-activ_enz_E1. IPR000011. UBQ-activ_enz_E1-like. IPR018074. UBQ-activ_enz_E1_AS. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
Pfam	PF00899. ThiF. 2 hits. PF09358. UBA_e1_C. 1 hit. PF10585. UBA_e1_thiolCys. 1 hit. PF02134. UBACT. 2 hits. [Graphical view]
PRINTS	PR01849. UBIQUITINACT.
TIGRFAMs	TIGR01408. Ube1. 1 hit.
PROSITE	PS00536. UBIQUITIN_ACTIVAT_1. 1 hit. PS00865. UBIQUITIN_ACTIVAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

O46111_DROME

Accession

Primary (citable) accession number: O46111

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)