O46036 (CTBP_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
C-terminal-binding protein
Short name=CtBP protein

Alternative name(s):
dCtBP

Gene names

Name:	CtBP
ORF Names:	CG7583

Organism

Drosophila melanogaster (Fruit fly)

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Corepressor targeting diverse transcription regulators. Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression. Ref.1 Ref.2
Subunit structure	Homodimer. Interacts with hairy (h), knirps (kni), snail (sna), and Enhancer of split m-delta (HLHm-delta). Complex may be involved in transcriptional repression. Interacts also with adenovirus E1A protein. Ref.1
Subcellular location	Nucleus.
Developmental stage	Expressed both maternally and zygotically. Zygotic expression is highest in pupae. Ref.1
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Coding sequence diversity	Alternative splicing
Molecular function	Oxidoreductase Repressor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Wnt receptor signaling pathway Inferred from genetic interaction. Source: FlyBase chaeta development Inferred from mutant phenotype. Source: FlyBase embryonic development via the syncytial blastoderm Inferred from mutant phenotype. Source: FlyBase negative regulation of transcription from RNA polymerase II promoter Traceable author statement. Source: FlyBase regulation of Wnt receptor signaling pathway Inferred from mutant phenotype. Source: FlyBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW wing disc development Inferred from mutant phenotype. Source: FlyBase
Cellular component	nucleus Inferred from direct assay. Source: FlyBase
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Inferred from electronic annotation. Source: InterPro protein homodimerization activity Inferred from direct assay. Source: FlyBase transcription coactivator activity Inferred from direct assay. Source: FlyBase transcription corepressor activity Inferred from mutant phenotype. Source: FlyBase transcription factor binding Inferred from physical interaction. Source: FlyBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
h	P14003	2	EBI-159330,EBI-123011
kni	P10734	2	EBI-159330,EBI-170297

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Note: A number of isoforms are produced.

Isoform E (identifier: O46036-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform A (identifier: O46036-2)

Also known as: B;

The sequence of this isoform differs from the canonical sequence as follows:
     377-386: ALHHRAHSTT → KLQMISNQEK
     387-476: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 476	476	C-terminal-binding protein		PRO_0000076048
Regions
	Nucleotide binding	180 – 185	6	NAD By similarity
	Nucleotide binding	237 – 243	7	NAD By similarity
	Nucleotide binding	264 – 266	3	NAD By similarity
	Nucleotide binding	315 – 318	4	NAD By similarity
	Compositional bias	362 – 365	4	Poly-Ala
Sites
	Active site	266	1	By similarity
	Active site	295	1	By similarity
	Active site	315	1	Proton donor By similarity
	Binding site	100	1	NAD By similarity
	Binding site	204	1	NAD By similarity
	Binding site	290	1	NAD By similarity
Natural variations
	Alternative sequence	377 – 386	10	ALHHRAHSTT → KLQMISNQEK in isoform A.		VSP_011813
	Alternative sequence	387 – 476	90	Missing in isoform A.		VSP_011814
Experimental info
	Sequence conflict	299 – 301	3	Missing in BAA25287. Ref.2
	Sequence conflict	371	1	A → S in CAA12074. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform E [UniParc]. Last modified November 9, 2004. Version 3. Checksum: DD5F85535BEB56C1	FASTA	476	50,737
	10 20 30 40 50 60 MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT VAFCDAQSTS 70 80 90 100 110 120 EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS GTDNIDVKAA GELGIAVCNV 130 140 150 160 170 180 PGYGVEEVAD TTMCLILNLY RRTYWLANMV REGKKFTGPE QVREAAHGCA RIRGDTLGLV 190 200 210 220 230 240 GLGRIGSAVA LRAKAFGFNV IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN 250 260 270 280 290 300 EHNHHLINEF TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF 310 320 330 340 350 360 QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC VNKEYFMRTP 370 380 390 400 410 420 PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST VGGGPTTVAQ AAAAAVAAAA 430 440 450 460 470 AAALLPSPVP SHLSPQVGGL PLGIVSSQSP LSAPDPNNHL SSSIKTEVKA ESTEAP
	Isoform A (B) [UniParc]. Checksum: 89B9527BFF5224EC	FASTA	386	42,252
	10 20 30 40 50 60 MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT VAFCDAQSTS 70 80 90 100 110 120 EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS GTDNIDVKAA GELGIAVCNV 130 140 150 160 170 180 PGYGVEEVAD TTMCLILNLY RRTYWLANMV REGKKFTGPE QVREAAHGCA RIRGDTLGLV 190 200 210 220 230 240 GLGRIGSAVA LRAKAFGFNV IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN 250 260 270 280 290 300 EHNHHLINEF TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF 310 320 330 340 350 360 QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC VNKEYFMRTP 370 380 PAAAAGGVAA AVYPEGKLQM ISNQEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Drosophila CtBP: a Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression." Poortinga G., Watanabe M., Parkhurst S.M. EMBO J. 17:2067-2078(1998) [PubMed: 9524128] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, INTERACTION WITH H AND HLHM-DELTA, DEVELOPMENTAL STAGE, ALTERNATIVE SPLICING. Tissue: Embryo.
[2]	"Interaction of short-range repressors with Drosophila CtBP in the embryo." Nibu Y., Zhang H., Levine M. Science 280:101-104(1998) [PubMed: 9525852] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION.
[3]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[4]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[5]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed: 12537569] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A), NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 319-476 (ISOFORM E). Strain: Berkeley. Tissue: Head.
[6]	Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A. , Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E. Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ224690 mRNA. Translation: CAA12074.1. AB011840 mRNA. Translation: BAA25287.1. AE014297 Genomic DNA. Translation: AAF54891.1. AE014297 Genomic DNA. Translation: AAX52947.1. AY060646 mRNA. Translation: AAL28194.2. AY069170 mRNA. Translation: AAL39315.1.
RefSeq	NP_001014617.1. NM_001014617.1. NP_524336.2. NM_079612.2. NP_731762.1. NM_169490.1. NP_731763.1. NM_169491.1. NP_731764.1. NM_169492.1.
UniGene	Dm.2246.
3D structure databases
ProteinModelPortal	O46036.
SMR	O46036. Positions 28-352.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-17268N.
IntAct	O46036. 21 interactions.
MINT	MINT-303186.
STRING	O46036.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0100161; FBpp0099514; FBgn0020496.
GeneID	41602.
KEGG	dme:Dmel_CG7583.
Organism-specific databases
CTD	41602.
FlyBase	FBgn0020496. CtBP.
Phylogenomic databases
eggNOG	inNOG04466.
GeneTree	EMGT00050000017176.
InParanoid	O46036.
OMA	YPEGALH.
OrthoDB	EOG41893S.
PhylomeDB	O46036.
Gene expression databases
Bgee	O46036.
GermOnline	CG7583. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006139. D-isomer_2_OHA_DH_cat_dom. IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 2 hits.
KO	K04496.
Pfam	PF00389. 2-Hacid_dh. 1 hit. PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	824592.

Entry information

Entry name

CTBP_DROME

Accession

Primary (citable) accession number: O46036
Secondary accession number(s): O61283 , Q59DX5, Q95SQ8, Q9VG02

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 1, 2000
Last sequence update:	November 9, 2004
Last modified:	January 25, 2012
This is version 111 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project