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Protein

C-terminal-binding protein

Gene

CtBP

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei204NADBy similarity1
Active sitei266By similarity1
Binding sitei290NADBy similarity1
Active sitei295By similarity1
Active sitei315Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 185NADBy similarity6
Nucleotide bindingi237 – 243NADBy similarity7
Nucleotide bindingi264 – 266NADBy similarity3
Nucleotide bindingi315 – 318NADBy similarity4

GO - Molecular functioni

  • NAD binding Source: InterPro
  • oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
  • protein homodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • transcription coactivator activity Source: FlyBase
  • transcription corepressor activity Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: FlyBase
  • chaeta development Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • positive regulation of JAK-STAT cascade Source: FlyBase
  • regulation of transcription, DNA-templated Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • regulation of Wnt signaling pathway Source: FlyBase
  • sensory organ precursor cell fate determination Source: FlyBase
  • sensory perception of pain Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • wing disc development Source: FlyBase
  • Wnt signaling pathway Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase, Repressor

Keywords - Biological processi

Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-3769402. Deactivation of the beta-catenin transactivating complex.
R-DME-4641265. Repression of WNT target genes.
SignaLinkiO46036.

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein
Short name:
CtBP protein
Alternative name(s):
dCtBP
Gene namesi
Name:CtBP
ORF Names:CG7583
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0020496. CtBP.

Subcellular locationi

GO - Cellular componenti

  • ISWI-type complex Source: FlyBase
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760481 – 476C-terminal-binding proteinAdd BLAST476

Proteomic databases

PaxDbiO46036.
PRIDEiO46036.

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Zygotic expression is highest in pupae.1 Publication

Gene expression databases

BgeeiFBgn0020496.
ExpressionAtlasiO46036. baseline.
GenevisibleiO46036. DM.

Interactioni

Subunit structurei

Homodimer. Interacts with hairy (h), knirps (kni), snail (sna), and Enhancer of split m-delta (HLHm-delta). Complex may be involved in transcriptional repression. Interacts also with adenovirus E1A protein.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself3EBI-159330,EBI-159330
hP140034EBI-159330,EBI-123011
kniP107342EBI-159330,EBI-170297
snaP080444EBI-159330,EBI-152305

GO - Molecular functioni

  • protein homodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi66690. 63 interactors.
DIPiDIP-17268N.
IntActiO46036. 27 interactors.
MINTiMINT-303186.
STRINGi7227.FBpp0099514.

Structurei

3D structure databases

ProteinModelPortaliO46036.
SMRiO46036.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi362 – 365Poly-Ala4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
InParanoidiO46036.
KOiK04496.
OrthoDBiEOG091G08GS.
PhylomeDBiO46036.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform E (identifier: O46036-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT
60 70 80 90 100
VAFCDAQSTS EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS
110 120 130 140 150
GTDNIDVKAA GELGIAVCNV PGYGVEEVAD TTMCLILNLY RRTYWLANMV
160 170 180 190 200
REGKKFTGPE QVREAAHGCA RIRGDTLGLV GLGRIGSAVA LRAKAFGFNV
210 220 230 240 250
IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN EHNHHLINEF
260 270 280 290 300
TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF
310 320 330 340 350
QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC
360 370 380 390 400
VNKEYFMRTP PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST
410 420 430 440 450
VGGGPTTVAQ AAAAAVAAAA AAALLPSPVP SHLSPQVGGL PLGIVSSQSP
460 470
LSAPDPNNHL SSSIKTEVKA ESTEAP
Note: No experimental confirmation available.
Length:476
Mass (Da):50,737
Last modified:November 9, 2004 - v3
Checksum:iDD5F85535BEB56C1
GO
Isoform A (identifier: O46036-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     377-386: ALHHRAHSTT → KLQMISNQEK
     387-476: Missing.

Show »
Length:386
Mass (Da):42,252
Checksum:i89B9527BFF5224EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299 – 301Missing in BAA25287 (PubMed:9525852).Curated3
Sequence conflicti371A → S in CAA12074 (PubMed:9524128).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011813377 – 386ALHHRAHSTT → KLQMISNQEK in isoform A. 3 Publications10
Alternative sequenceiVSP_011814387 – 476Missing in isoform A. 3 PublicationsAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224690 mRNA. Translation: CAA12074.1.
AB011840 mRNA. Translation: BAA25287.1.
AE014297 Genomic DNA. Translation: AAF54891.1.
AE014297 Genomic DNA. Translation: AAX52947.1.
AY060646 mRNA. Translation: AAL28194.2.
AY069170 mRNA. Translation: AAL39315.1.
RefSeqiNP_001014617.1. NM_001014617.2. [O46036-1]
NP_001262520.1. NM_001275591.1.
NP_524336.2. NM_079612.3. [O46036-2]
NP_731762.1. NM_169490.2. [O46036-2]
NP_731763.1. NM_169491.2. [O46036-2]
NP_731764.1. NM_169492.2. [O46036-2]
UniGeneiDm.2246.

Genome annotation databases

EnsemblMetazoaiFBtr0100161; FBpp0099514; FBgn0020496. [O46036-1]
GeneIDi41602.
KEGGidme:Dmel_CG7583.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224690 mRNA. Translation: CAA12074.1.
AB011840 mRNA. Translation: BAA25287.1.
AE014297 Genomic DNA. Translation: AAF54891.1.
AE014297 Genomic DNA. Translation: AAX52947.1.
AY060646 mRNA. Translation: AAL28194.2.
AY069170 mRNA. Translation: AAL39315.1.
RefSeqiNP_001014617.1. NM_001014617.2. [O46036-1]
NP_001262520.1. NM_001275591.1.
NP_524336.2. NM_079612.3. [O46036-2]
NP_731762.1. NM_169490.2. [O46036-2]
NP_731763.1. NM_169491.2. [O46036-2]
NP_731764.1. NM_169492.2. [O46036-2]
UniGeneiDm.2246.

3D structure databases

ProteinModelPortaliO46036.
SMRiO46036.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi66690. 63 interactors.
DIPiDIP-17268N.
IntActiO46036. 27 interactors.
MINTiMINT-303186.
STRINGi7227.FBpp0099514.

Proteomic databases

PaxDbiO46036.
PRIDEiO46036.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0100161; FBpp0099514; FBgn0020496. [O46036-1]
GeneIDi41602.
KEGGidme:Dmel_CG7583.

Organism-specific databases

CTDi41602.
FlyBaseiFBgn0020496. CtBP.

Phylogenomic databases

eggNOGiKOG0067. Eukaryota.
COG0111. LUCA.
GeneTreeiENSGT00530000063021.
InParanoidiO46036.
KOiK04496.
OrthoDBiEOG091G08GS.
PhylomeDBiO46036.

Enzyme and pathway databases

ReactomeiR-DME-3769402. Deactivation of the beta-catenin transactivating complex.
R-DME-4641265. Repression of WNT target genes.
SignaLinkiO46036.

Miscellaneous databases

ChiTaRSiCtBP. fly.
GenomeRNAii41602.
PROiO46036.

Gene expression databases

BgeeiFBgn0020496.
ExpressionAtlasiO46036. baseline.
GenevisibleiO46036. DM.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR006139. D-isomer_2_OHA_DH_cat_dom.
IPR029753. D-isomer_DH_CS.
IPR006140. D-isomer_DH_NAD-bd.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PfamiPF00389. 2-Hacid_dh. 1 hit.
PF02826. 2-Hacid_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00671. D_2_HYDROXYACID_DH_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCTBP_DROME
AccessioniPrimary (citable) accession number: O46036
Secondary accession number(s): O61283
, Q59DX5, Q95SQ8, Q9VG02
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 9, 2004
Last modified: November 30, 2016
This is version 155 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.