Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

C-terminal-binding protein

Gene

CtBP

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Corepressor targeting diverse transcription regulators. Hairy-interacting protein required for embryonic segmentation and hairy-mediated transcriptional repression.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei100NADBy similarity1
Binding sitei204NADBy similarity1
Active sitei266By similarity1
Binding sitei290NADBy similarity1
Active sitei295By similarity1
Active sitei315Proton donorBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi180 – 185NADBy similarity6
Nucleotide bindingi237 – 243NADBy similarity7
Nucleotide bindingi264 – 266NADBy similarity3
Nucleotide bindingi315 – 318NADBy similarity4

GO - Molecular functioni

  • glyoxylate reductase (NADP) activity Source: GO_Central
  • hydroxypyruvate reductase activity Source: GO_Central
  • identical protein binding Source: IntAct
  • NAD binding Source: InterPro
  • protein homodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • transcription coactivator activity Source: FlyBase
  • transcription corepressor activity Source: FlyBase
  • transcription factor binding Source: FlyBase

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: FlyBase
  • chaeta development Source: FlyBase
  • embryonic development via the syncytial blastoderm Source: FlyBase
  • negative regulation of canonical Wnt signaling pathway Source: FlyBase
  • negative regulation of transcription, DNA-templated Source: FlyBase
  • negative regulation of transcription by RNA polymerase II Source: FlyBase
  • positive regulation of canonical Wnt signaling pathway Source: FlyBase
  • positive regulation of transcription by RNA polymerase II Source: FlyBase
  • regulation of transcription by RNA polymerase II Source: FlyBase
  • sensory organ precursor cell fate determination Source: FlyBase
  • transcription, DNA-templated Source: UniProtKB-KW
  • wing disc development Source: FlyBase

Keywordsi

Molecular functionOxidoreductase, Repressor
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-3769402 Deactivation of the beta-catenin transactivating complex
R-DME-4641265 Repression of WNT target genes
SignaLinkiO46036

Names & Taxonomyi

Protein namesi
Recommended name:
C-terminal-binding protein
Short name:
CtBP protein
Alternative name(s):
dCtBP
Gene namesi
Name:CtBP
ORF Names:CG7583
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 3R

Organism-specific databases

FlyBaseiFBgn0020496 CtBP

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000760481 – 476C-terminal-binding proteinAdd BLAST476

Proteomic databases

PaxDbiO46036
PRIDEiO46036

Expressioni

Developmental stagei

Expressed both maternally and zygotically. Zygotic expression is highest in pupae.1 Publication

Gene expression databases

BgeeiFBgn0020496
ExpressionAtlasiO46036 differential
GenevisibleiO46036 DM

Interactioni

Subunit structurei

Homodimer. Interacts with hairy (h), knirps (kni), snail (sna), and Enhancer of split m-delta (HLHm-delta). Complex may be involved in transcriptional repression. Interacts also with adenovirus E1A protein.1 Publication

Binary interactionsi

Show more details

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: FlyBase
  • repressing transcription factor binding Source: FlyBase
  • transcription factor binding Source: FlyBase

Protein-protein interaction databases

BioGridi66690, 78 interactors
DIPiDIP-17268N
IntActiO46036, 52 interactors
MINTiO46036
STRINGi7227.FBpp0099514

Structurei

3D structure databases

ProteinModelPortaliO46036
SMRiO46036
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi362 – 365Poly-Ala4

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0067 Eukaryota
COG0111 LUCA
GeneTreeiENSGT00530000063021
InParanoidiO46036
KOiK04496
OrthoDBiEOG091G08GS
PhylomeDBiO46036

Family and domain databases

InterProiView protein in InterPro
IPR006139 D-isomer_2_OHA_DH_cat_dom
IPR029753 D-isomer_DH_CS
IPR006140 D-isomer_DH_NAD-bd
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF00389 2-Hacid_dh, 1 hit
PF02826 2-Hacid_dh_C, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
PROSITEiView protein in PROSITE
PS00671 D_2_HYDROXYACID_DH_3, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.
Isoform E (identifier: O46036-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDKNLMMPKR SRIDVKGNFA NGPLQARPLV ALLDGRDCSI EMPILKDVAT
60 70 80 90 100
VAFCDAQSTS EIHEKVLNEA VGALMWHTII LTKEDLEKFK ALRIIVRIGS
110 120 130 140 150
GTDNIDVKAA GELGIAVCNV PGYGVEEVAD TTMCLILNLY RRTYWLANMV
160 170 180 190 200
REGKKFTGPE QVREAAHGCA RIRGDTLGLV GLGRIGSAVA LRAKAFGFNV
210 220 230 240 250
IFYDPYLPDG IDKSLGLTRV YTLQDLLFQS DCVSLHCTLN EHNHHLINEF
260 270 280 290 300
TIKQMRPGAF LVNTARGGLV DDETLALALK QGRIRAAALD VHENEPYNVF
310 320 330 340 350
QGALKDAPNL ICTPHAAFFS DASATELREM AATEIRRAIV GNIPDVLRNC
360 370 380 390 400
VNKEYFMRTP PAAAAGGVAA AVYPEGALHH RAHSTTPHDG PHSTTNLGST
410 420 430 440 450
VGGGPTTVAQ AAAAAVAAAA AAALLPSPVP SHLSPQVGGL PLGIVSSQSP
460 470
LSAPDPNNHL SSSIKTEVKA ESTEAP
Note: No experimental confirmation available.
Length:476
Mass (Da):50,737
Last modified:November 9, 2004 - v3
Checksum:iDD5F85535BEB56C1
GO
Isoform A (identifier: O46036-2) [UniParc]FASTAAdd to basket
Also known as: B

The sequence of this isoform differs from the canonical sequence as follows:
     377-386: ALHHRAHSTT → KLQMISNQEK
     387-476: Missing.

Show »
Length:386
Mass (Da):42,252
Checksum:i89B9527BFF5224EC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti299 – 301Missing in BAA25287 (PubMed:9525852).Curated3
Sequence conflicti371A → S in CAA12074 (PubMed:9524128).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_011813377 – 386ALHHRAHSTT → KLQMISNQEK in isoform A. 3 Publications10
Alternative sequenceiVSP_011814387 – 476Missing in isoform A. 3 PublicationsAdd BLAST90

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ224690 mRNA Translation: CAA12074.1
AB011840 mRNA Translation: BAA25287.1
AE014297 Genomic DNA Translation: AAF54891.1
AE014297 Genomic DNA Translation: AAX52947.1
AY060646 mRNA Translation: AAL28194.2
AY069170 mRNA Translation: AAL39315.1
RefSeqiNP_001014617.1, NM_001014617.2 [O46036-1]
NP_001262520.1, NM_001275591.1
NP_524336.2, NM_079612.3 [O46036-2]
NP_731762.1, NM_169490.2 [O46036-2]
NP_731763.1, NM_169491.2 [O46036-2]
NP_731764.1, NM_169492.2 [O46036-2]
UniGeneiDm.2246

Genome annotation databases

EnsemblMetazoaiFBtr0082699; FBpp0082167; FBgn0020496 [O46036-2]
FBtr0082700; FBpp0082168; FBgn0020496 [O46036-2]
FBtr0082701; FBpp0082169; FBgn0020496 [O46036-2]
FBtr0082702; FBpp0082170; FBgn0020496 [O46036-2]
FBtr0100161; FBpp0099514; FBgn0020496 [O46036-1]
GeneIDi41602
KEGGidme:Dmel_CG7583

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiCTBP_DROME
AccessioniPrimary (citable) accession number: O46036
Secondary accession number(s): O61283
, Q59DX5, Q95SQ8, Q9VG02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: November 9, 2004
Last modified: March 28, 2018
This is version 166 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health