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O45947

- GLT10_CAEEL

UniProt

O45947 - GLT10_CAEEL

Protein

Putative polypeptide N-acetylgalactosaminyltransferase 10

Gene

gly-10

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 3 (08 Apr 2008)
      Previous versions | rss
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    Functioni

    May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei259 – 2591SubstrateBy similarity
    Binding sitei291 – 2911SubstrateBy similarity
    Metal bindingi314 – 3141ManganeseBy similarity
    Metal bindingi316 – 3161ManganeseBy similarity
    Binding sitei417 – 4171SubstrateBy similarity
    Metal bindingi445 – 4451ManganeseBy similarity
    Binding sitei448 – 4481SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiCBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)
    Short name:
    pp-GaNTase 10
    Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 10
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
    Gene namesi
    Name:gly-10
    ORF Names:Y45F10D.3
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiY45F10D.3a; CE16642; WBGene00001635; gly-10.
    Y45F10D.3b; CE39841; WBGene00001635; gly-10.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 684684Putative polypeptide N-acetylgalactosaminyltransferase 10PRO_0000059153Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi198 – 1981N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi209 ↔ 440PROSITE-ProRule annotation
    Glycosylationi232 – 2321N-linked (GlcNAc...)1 Publication
    Disulfide bondi431 ↔ 509PROSITE-ProRule annotation
    Disulfide bondi548 ↔ 565PROSITE-ProRule annotation
    Disulfide bondi594 ↔ 611PROSITE-ProRule annotation
    Disulfide bondi637 ↔ 653PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO45947.

    Interactioni

    Protein-protein interaction databases

    STRINGi6239.Y45F10D.3a.

    Structurei

    3D structure databases

    ProteinModelPortaliO45947.
    SMRiO45947. Positions 138-678.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 7474CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini96 – 684589LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei75 – 9521Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini581 – 684104Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni218 – 330113Catalytic subdomain AAdd
    BLAST
    Regioni386 – 44863Catalytic subdomain BAdd
    BLAST
    Regioni448 – 46114Flexible loopBy similarityAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239675.
    GeneTreeiENSGT00740000115054.
    HOGENOMiHOG000038227.
    InParanoidiO45947.
    KOiK00710.
    OMAiQIYHPIS.
    OrthoDBiEOG7J9VP2.
    PhylomeDBiO45947.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform a (identifier: O45947-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MVAICIKIGE RERKRVHIML LAYTWKTRVS SHQCNKSPIF GLFAIQFSNI    50
    CSNLLLLQQK LSMGLSRYLS RRHHWVIQYC GLLLFLYLIY SYVATSNDGP 100
    NLHEDIPVFQ GQGKDRANPN PPAALGDEAL DPFEKYRGHE KIKWEDEAAY 150
    EKEKRREGPG EWGKPVKLPE DKEVEKEALS LYKANGYNAY ISDMISLNRS 200
    IKDIRHKECK NMMYSAKLPT VSVIFPFHEE HNSTLLRSVY SVINRSPPEL 250
    LKEIILVDDF SEKPALRQPL EDFLKKNKID HIVKVLRTKK REGLIRGRQL 300
    GAQDATGEIL IFLDAHSEAN YNWLPPLLDP IAEDYRTVVC PFVDVIDCET 350
    YEVRPQDEGA RGSFDWAFNY KRLPLTKKDR ESPTKPFNSP VMAGGYFAIS 400
    AKWFWELGGY DEGLDIWGGE QYELSFKVWQ CHGRMVDAPC SRVAHIYRCK 450
    YAPFKNAGMG DFVSRNYKRV AEVWMDDYKE TLYKHRPGVG NADAGDLKLM 500
    KGIREKLQCK SFDWFMKEIA FDQDKYYPAV EPKASAEGEI RNVGTNFCID 550
    TQFKEQNQRF GLRKCTSDDK DGGGEQDLRL TRWHDIRPKG RKICFDCSTS 600
    VDKAPVILFD CHSMKGNQLF KYRVAQKQIY HPISGQCLTA DENGKGFLHM 650
    KKCDSSSDLQ KWAWQTVDNE LLETRQANEA KEQE 684

    Note: No experimental confirmation available.

    Length:684
    Mass (Da):78,971
    Last modified:April 8, 2008 - v3
    Checksum:i59B9B3F84C2B3445
    GO
    Isoform b (identifier: O45947-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-62: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:622
    Mass (Da):71,812
    Checksum:iF8A3A21159C7E452
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 6262Missing in isoform b. CuratedVSP_032724Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL021492 Genomic DNA. Translation: CAA16378.1.
    AL021492 Genomic DNA. Translation: CAJ76949.1.
    PIRiT26930.
    RefSeqiNP_001041036.1. NM_001047571.2. [O45947-1]
    NP_001041037.1. NM_001047572.2. [O45947-2]
    UniGeneiCel.6680.

    Genome annotation databases

    EnsemblMetazoaiY45F10D.3a; Y45F10D.3a; WBGene00001635. [O45947-1]
    GeneIDi178343.
    KEGGicel:CELE_Y45F10D.3.
    UCSCiY45F10D.3a. c. elegans. [O45947-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL021492 Genomic DNA. Translation: CAA16378.1 .
    AL021492 Genomic DNA. Translation: CAJ76949.1 .
    PIRi T26930.
    RefSeqi NP_001041036.1. NM_001047571.2. [O45947-1 ]
    NP_001041037.1. NM_001047572.2. [O45947-2 ]
    UniGenei Cel.6680.

    3D structure databases

    ProteinModelPortali O45947.
    SMRi O45947. Positions 138-678.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6239.Y45F10D.3a.

    Protein family/group databases

    CAZyi CBM13. Carbohydrate-Binding Module Family 13.
    GT27. Glycosyltransferase Family 27.

    Proteomic databases

    PaxDbi O45947.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai Y45F10D.3a ; Y45F10D.3a ; WBGene00001635 . [O45947-1 ]
    GeneIDi 178343.
    KEGGi cel:CELE_Y45F10D.3.
    UCSCi Y45F10D.3a. c. elegans. [O45947-1 ]

    Organism-specific databases

    CTDi 178343.
    WormBasei Y45F10D.3a ; CE16642 ; WBGene00001635 ; gly-10.
    Y45F10D.3b ; CE39841 ; WBGene00001635 ; gly-10.

    Phylogenomic databases

    eggNOGi NOG239675.
    GeneTreei ENSGT00740000115054.
    HOGENOMi HOG000038227.
    InParanoidi O45947.
    KOi K00710.
    OMAi QIYHPIS.
    OrthoDBi EOG7J9VP2.
    PhylomeDBi O45947.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 900740.
    PROi O45947.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
      Strain: Bristol N2.
    2. "The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation."
      Wang H., Spang A., Sullivan M.A., Hryhorenko J., Hagen F.K.
      Mol. Biol. Cell 16:4202-4213(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION.
    3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-232, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGLT10_CAEEL
    AccessioniPrimary (citable) accession number: O45947
    Secondary accession number(s): Q2HQM0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: April 8, 2008
    Last modified: October 1, 2014
    This is version 107 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3