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O45947

- GLT10_CAEEL

UniProt

O45947 - GLT10_CAEEL

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Protein
Putative polypeptide N-acetylgalactosaminyltransferase 10
Gene
gly-10, Y45F10D.3
Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor By similarity.

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Manganese By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei259 – 2591Substrate By similarity
Binding sitei291 – 2911Substrate By similarity
Metal bindingi314 – 3141Manganese By similarity
Metal bindingi316 – 3161Manganese By similarity
Binding sitei417 – 4171Substrate By similarity
Metal bindingi445 – 4451Manganese By similarity
Binding sitei448 – 4481Substrate By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
  2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Protein family/group databases

CAZyiCBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)
Short name:
pp-GaNTase 10
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 10
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
Gene namesi
Name:gly-10
ORF Names:Y45F10D.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiY45F10D.3a; CE16642; WBGene00001635; gly-10.
Y45F10D.3b; CE39841; WBGene00001635; gly-10.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 7474Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei75 – 9521Helical; Signal-anchor for type II membrane protein; Reviewed prediction
Add
BLAST
Topological domaini96 – 684589Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. Golgi membrane Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 684684Putative polypeptide N-acetylgalactosaminyltransferase 10
PRO_0000059153Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi198 – 1981N-linked (GlcNAc...) Reviewed prediction
Disulfide bondi209 ↔ 440 By similarity
Glycosylationi232 – 2321N-linked (GlcNAc...)1 Publication
Disulfide bondi431 ↔ 509 By similarity
Disulfide bondi548 ↔ 565 By similarity
Disulfide bondi594 ↔ 611 By similarity
Disulfide bondi637 ↔ 653 By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO45947.

Interactioni

Protein-protein interaction databases

STRINGi6239.Y45F10D.3a.

Structurei

3D structure databases

ProteinModelPortaliO45947.
SMRiO45947. Positions 138-678.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini581 – 684104Ricin B-type lectin
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni218 – 330113Catalytic subdomain A
Add
BLAST
Regioni386 – 44863Catalytic subdomain B
Add
BLAST
Regioni448 – 46114Flexible loop By similarity
Add
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding By similarity.
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity By similarity.

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
GeneTreeiENSGT00740000115054.
HOGENOMiHOG000038227.
InParanoidiO45947.
KOiK00710.
OMAiQIYHPIS.
OrthoDBiEOG7J9VP2.
PhylomeDBiO45947.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform a (identifier: O45947-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MVAICIKIGE RERKRVHIML LAYTWKTRVS SHQCNKSPIF GLFAIQFSNI    50
CSNLLLLQQK LSMGLSRYLS RRHHWVIQYC GLLLFLYLIY SYVATSNDGP 100
NLHEDIPVFQ GQGKDRANPN PPAALGDEAL DPFEKYRGHE KIKWEDEAAY 150
EKEKRREGPG EWGKPVKLPE DKEVEKEALS LYKANGYNAY ISDMISLNRS 200
IKDIRHKECK NMMYSAKLPT VSVIFPFHEE HNSTLLRSVY SVINRSPPEL 250
LKEIILVDDF SEKPALRQPL EDFLKKNKID HIVKVLRTKK REGLIRGRQL 300
GAQDATGEIL IFLDAHSEAN YNWLPPLLDP IAEDYRTVVC PFVDVIDCET 350
YEVRPQDEGA RGSFDWAFNY KRLPLTKKDR ESPTKPFNSP VMAGGYFAIS 400
AKWFWELGGY DEGLDIWGGE QYELSFKVWQ CHGRMVDAPC SRVAHIYRCK 450
YAPFKNAGMG DFVSRNYKRV AEVWMDDYKE TLYKHRPGVG NADAGDLKLM 500
KGIREKLQCK SFDWFMKEIA FDQDKYYPAV EPKASAEGEI RNVGTNFCID 550
TQFKEQNQRF GLRKCTSDDK DGGGEQDLRL TRWHDIRPKG RKICFDCSTS 600
VDKAPVILFD CHSMKGNQLF KYRVAQKQIY HPISGQCLTA DENGKGFLHM 650
KKCDSSSDLQ KWAWQTVDNE LLETRQANEA KEQE 684

Note: No experimental confirmation available.

Length:684
Mass (Da):78,971
Last modified:April 8, 2008 - v3
Checksum:i59B9B3F84C2B3445
GO
Isoform b (identifier: O45947-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-62: Missing.

Note: No experimental confirmation available.

Show »
Length:622
Mass (Da):71,812
Checksum:iF8A3A21159C7E452
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 6262Missing in isoform b.
VSP_032724Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021492 Genomic DNA. Translation: CAA16378.1.
AL021492 Genomic DNA. Translation: CAJ76949.1.
PIRiT26930.
RefSeqiNP_001041036.1. NM_001047571.2. [O45947-1]
NP_001041037.1. NM_001047572.2. [O45947-2]
UniGeneiCel.6680.

Genome annotation databases

EnsemblMetazoaiY45F10D.3a; Y45F10D.3a; WBGene00001635. [O45947-1]
GeneIDi178343.
KEGGicel:CELE_Y45F10D.3.
UCSCiY45F10D.3a. c. elegans. [O45947-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AL021492 Genomic DNA. Translation: CAA16378.1 .
AL021492 Genomic DNA. Translation: CAJ76949.1 .
PIRi T26930.
RefSeqi NP_001041036.1. NM_001047571.2. [O45947-1 ]
NP_001041037.1. NM_001047572.2. [O45947-2 ]
UniGenei Cel.6680.

3D structure databases

ProteinModelPortali O45947.
SMRi O45947. Positions 138-678.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6239.Y45F10D.3a.

Protein family/group databases

CAZyi CBM13. Carbohydrate-Binding Module Family 13.
GT27. Glycosyltransferase Family 27.

Proteomic databases

PaxDbi O45947.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai Y45F10D.3a ; Y45F10D.3a ; WBGene00001635 . [O45947-1 ]
GeneIDi 178343.
KEGGi cel:CELE_Y45F10D.3.
UCSCi Y45F10D.3a. c. elegans. [O45947-1 ]

Organism-specific databases

CTDi 178343.
WormBasei Y45F10D.3a ; CE16642 ; WBGene00001635 ; gly-10.
Y45F10D.3b ; CE39841 ; WBGene00001635 ; gly-10.

Phylogenomic databases

eggNOGi NOG239675.
GeneTreei ENSGT00740000115054.
HOGENOMi HOG000038227.
InParanoidi O45947.
KOi K00710.
OMAi QIYHPIS.
OrthoDBi EOG7J9VP2.
PhylomeDBi O45947.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Miscellaneous databases

NextBioi 900740.
PROi O45947.

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], ALTERNATIVE SPLICING.
    Strain: Bristol N2.
  2. "The terminal phase of cytokinesis in the Caenorhabditis elegans early embryo requires protein glycosylation."
    Wang H., Spang A., Sullivan M.A., Hryhorenko J., Hagen F.K.
    Mol. Biol. Cell 16:4202-4213(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
    Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
    Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-232, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Bristol N2.

Entry informationi

Entry nameiGLT10_CAEEL
AccessioniPrimary (citable) accession number: O45947
Secondary accession number(s): Q2HQM0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 16, 2004
Last sequence update: April 8, 2008
Last modified: July 9, 2014
This is version 106 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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