Reviewed, UniProtKB/Swiss-Prot O45870 (NSMA_CAEEL)

Names and origin

Protein names

Recommended name:
    Putative neutral sphingomyelinase
    EC=3.1.4.12

Gene names

ORF Names:

T27F6.6

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	434 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the neutral sphingomyelinase family.

Ontologies

Keywords
Cellular component	Membrane
Domain	Transmembrane
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 434	434	Putative neutral sphingomyelinase		PRO_0000075689
Regions
	Transmembrane	366 – 388	23	Potential
	Transmembrane	392 – 414	23	Potential
Sites
	Active site	318	1	Proton acceptor By similarity
	Metal binding	83	1	Magnesium By similarity
	Site	215	1	Important for substrate recognition By similarity

Sequences

	Sequence		Length	Mass (Da)
	O45870-1 [UniParc]. Last modified July 25, 2003. Version 2. Checksum: 5AE68CAE361977FB	FASTA	434	49,864
	10 20 30 40 50 60 MEPSSEQESQ ESQKVDMEPV FRRESFAALR RNNVAARQLR VVTLNAWCLP QPWPIGSTDR 70 80 90 100 110 120 VHRLNKIGQY MIDELYDIVG LQELWSYYDF VRLSEQVSSV YPYFHYFHSG FTGSGVCVFS 130 140 150 160 170 180 RHPIVSTLTN RYSLNGFAHH IHRGDWFGGK VVGLTEIEID GDLRVNFYTT HLHAEYDREN 190 200 210 220 230 240 DLYLPHRTAQ AFELAQFVRH TARGADVVIV TGDLNMEPCD LGFRLILSHA KLFDAWRMSH 250 260 270 280 290 300 EVENEDSEGE LLKFRGIAKG GTCDRPDNCY TKRALKNVDD SKRIDYMLFK SGRCNVKLEE 310 320 330 340 350 360 CEITLNQIPG EDLNYSDHVG LRARFTIDDR FRHEKSVNTW EPNRPLLIEA IGLVAGGERR 370 380 390 400 410 420 ARTDRIFFFI LAVICLILIL GSLFFEVFPM GFAVLRFALT VVGVFFVWQG LIGLTLERKA 430 LKAAKQAIQQ ILNN

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z82060 Genomic DNA. Translation: CAB04885.2.
PIR	T25386.
RefSeq	NP_493169.2.
UniGene	Cel.8884
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O45870.
Genome annotation databases
Ensembl	T27F6.6; T27F6.6; T27F6.6; Caenorhabditis elegans. [Genome view]
GeneID	173118.
KEGG	cel:T27F6.6.
UCSC	T27F6.6. c. elegans.
Organism-specific databases
CTD	173118.
WormBase	WBGene00012105. T27F6.6.
WormPep	T27F6.6. CE34214. [WorfDB]
Phylogenomic databases
OMA	HGILAGK.
Enzyme and pathway databases
BRENDA	3.1.4.12. 672.
Gene expression databases
ArrayExpress	O45870.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	878333.

Entry information

Entry name

NSMA_CAEEL

Accession

Primary (citable) accession number: O45870

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	July 25, 2003
Last modified:	November 3, 2009
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project