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Protein

Serine/threonine-protein kinase dkf-2

Gene

dkf-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Acts in the intestine to regulate both innate immunity by promoting activation of PMK-1 and also stress response and life span by acting as an upstream, negative regulator of the daf-16 transcription factor.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Enzyme regulationi

Activated by DAG and phorbol esters. Phorbol-ester/DAG-type domain 1 binds phorbol ester with low affinity. Phorbol-ester/DAG-type domain 2 binds phorbol ester with high affinity and targets the kinase to the cell periphery, enabling phosphorylation and activation by colocalized tpa-1. Both domains 1 and 2 appear to bind DAG with equal affinity so may contribute equally to translocation and activation.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei799ATPPROSITE-ProRule annotationBy similarity1
Active sitei893Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri321 – 371Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri473 – 523Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51
Nucleotide bindingi776 – 784ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • metal ion binding Source: UniProtKB-KW
  • protein homodimerization activity Source: WormBase
  • protein kinase activity Source: WormBase
  • protein kinase C activity Source: UniProtKB-EC
  • protein serine/threonine kinase activity Source: WormBase

GO - Biological processi

  • defense response to Gram-negative bacterium Source: WormBase
  • defense response to Gram-positive bacterium Source: WormBase
  • determination of adult lifespan Source: WormBase
  • innate immune response Source: WormBase
  • intracellular signal transduction Source: WormBase
  • negative regulation of protein import into nucleus Source: WormBase
  • peptidyl-serine phosphorylation Source: WormBase
  • phospholipase C-activating G-protein coupled receptor signaling pathway Source: WormBase
  • protein kinase D signaling Source: GO_Central
  • protein phosphorylation Source: WormBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processImmunity, Innate immunity
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-1660661 Sphingolipid de novo biosynthesis
SignaLinkiO45818

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase dkf-21 Publication (EC:2.7.11.13)
Alternative name(s):
D kinase family-21 Publication
Gene namesi
Name:dkf-2
ORF Names:T25E12.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiT25E12.4a ; CE42484 ; WBGene00012019 ; dkf-2
T25E12.4b ; CE52401 ; WBGene00012019 ; dkf-2
T25E12.4c ; CE42507 ; WBGene00012019 ; dkf-2
T25E12.4d ; CE46242 ; WBGene00012019 ; dkf-2
T25E12.4e ; CE47100 ; WBGene00012019 ; dkf-2
T25E12.4f ; CE47405 ; WBGene00012019 ; dkf-2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Increase in adult life span. Increased levels of daf-16 translocate into the nucleus in response to heat stress. Hypersensitive to killing by bacteria.2 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi332P → G: Loss of sensitivity to phorbol ester or DAG stimulation of kinase activity; when associated with G-484. No effect on sensitivity to phorbol ester, loss of DAG stimulation of kinase activity. 1 Publication1
Mutagenesisi484P → G: Loss of sensitivity to phorbol ester or DAG stimulation of kinase activity. Loss of sensitivity to phorbol ester or DAG stimulation of kinase activity; when associated with G-332. 1 Publication1
Mutagenesisi643K → A: Small increase in basal kinase activity, no effect on sensitivity to phorbol ester stimulation of kinase activity. 1 Publication1
Mutagenesisi925S → A: Loss of sensitivity to phorbol ester stimulation of kinase activity. 2 Publications1
Mutagenesisi925S → D: High basal kinase activity, loss of phorbol ester-stimulated kinase activity and ability to phosphorylate pmk-1; when associated with D-929. 2 Publications1
Mutagenesisi929S → A: Moderate loss of sensitivity to phorbol ester stimulation of kinase activity. 2 Publications1
Mutagenesisi929S → D: High basal kinase activity, loss of phorbol ester-stimulated kinase activity and ability to phosphorylate pmk-1; when associated with D-925. 2 Publications1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003853541 – 1070Serine/threonine-protein kinase dkf-2Add BLAST1070

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei925Phosphoserine1 Publication1
Modified residuei929Phosphoserine1 Publication1

Post-translational modificationi

Phosphorylation on Ser-925 by tpa-1 is the dominant regulator of catalysis, phosphorylation on Ser-929 by tpa-1 has a lesser effect. Prolonged phosphorylation results in ubiquitination and degradation.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiO45818
PeptideAtlasiO45818

PTM databases

iPTMnetiO45818

Expressioni

Tissue specificityi

Expressed in the late embryo, all larval stages, and adult in the intestine and in cells positioned near the posterior bulb of the pharynx.2 Publications

Gene expression databases

BgeeiWBGene00012019

Interactioni

GO - Molecular functioni

  • protein homodimerization activity Source: WormBase

Protein-protein interaction databases

STRINGi6239.T25E12.4a

Structurei

3D structure databases

ProteinModelPortaliO45818
SMRiO45818
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini624 – 726PHPROSITE-ProRule annotationAdd BLAST103
Domaini770 – 1026Protein kinasePROSITE-ProRule annotationAdd BLAST257

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi217 – 220Poly-SerSequence analysis4

Sequence similaritiesi

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri321 – 371Phorbol-ester/DAG-type 1PROSITE-ProRule annotationAdd BLAST51
Zinc fingeri473 – 523Phorbol-ester/DAG-type 2PROSITE-ProRule annotationAdd BLAST51

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG4236 Eukaryota
ENOG410XQZ3 LUCA
GeneTreeiENSGT00910000144054
HOGENOMiHOG000015135
InParanoidiO45818
KOiK06070
OMAiGEMQDPD
OrthoDBiEOG091G02RG
PhylomeDBiO45818

Family and domain databases

CDDicd00029 C1, 2 hits
Gene3Di2.30.29.30, 1 hit
InterProiView protein in InterPro
IPR020454 DAG/PE-bd
IPR011009 Kinase-like_dom_sf
IPR002219 PE/DAG-bd
IPR011993 PH-like_dom_sf
IPR001849 PH_domain
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR015727 Protein_Kinase_C_mu-related
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR22968 PTHR22968, 1 hit
PfamiView protein in Pfam
PF00130 C1_1, 2 hits
PF00169 PH, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR00008 DAGPEDOMAIN
SMARTiView protein in SMART
SM00109 C1, 2 hits
SM00233 PH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50003 PH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS00479 ZF_DAG_PE_1, 2 hits
PS50081 ZF_DAG_PE_2, 2 hits

Sequences (6)i

Sequence statusi: Complete.

This entry describes 6 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform a1 Publication (identifier: O45818-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDANDYPRLY YTSMPSSSTS MVTTTRFSTS FSPSIPCHRQ ENFRRHSTSA
60 70 80 90 100
LAKRNGSESE KSAEIRENPD EIEVSRVSGR DSSLAFYTTA HETSSMLSRD
110 120 130 140 150
SRDETLTPNE HIHQASSRRV SANDSVFEDG YVDFVDEPRE HGSRRKSFEK
160 170 180 190 200
FTDRNGEEKE GRVFELSTPQ PTREAAPGAH QFQLPTLLVT STPTTVFDHS
210 220 230 240 250
DDEVWTPYRP PPNREYSSSS EPMMGDLTFR LQSGIHKKSI AVEGTEIALR
260 270 280 290 300
DLRNEALQFI KEIYPEKGCS SLEDYILLYK HDLRSINILQ LITTSSDVTD
310 320 330 340 350
GTLVEVVIGS CPQNERIVVH PHTLFVHSYK VPTFCDFCGE LLFGLVKQGL
360 370 380 390 400
KCFGCGLNYH KRCASKIPNN CNGSKQRRPS AIPLSPSNSN ILNLNERRHS
410 420 430 440 450
RRESCLEALD AARPSSTLGG AATPNIFITS DDCGDAVGGN YLQMPRKDRS
460 470 480 490 500
CSWSGRPLWM EIAEATRVKL QVPHTFQVHS YKLPTVCQHC KKLLKGLIRQ
510 520 530 540 550
GMQCRDCKYN CHKKCSEHVA KDCSGNTKAS QFFLGSQADD GASEDRDDDL
560 570 580 590 600
SLRSGSGAHK KAQNTPSAPL QGSEGSGSPG PVVSFAANAL SNMPDDDVIS
610 620 630 640 650
SESANIPLMR VVMSKKQTKR KNNKLLKEGW IVHYTDQQNM RKKHYWRLDT
660 670 680 690 700
KGITMYQDEN TTRYYKEIPL NEILNVSMSP PDKTADYLFE IRTGVCVYFI
710 720 730 740 750
SGSPSDEKGS SLDAQSWTTA IQSALMPVTP QSSVVGGKRI DKLKVPTEGE
760 770 780 790 800
TGHLGAKIQT EHEFSQLYQI FAEEVLGSGQ FGTVYGGIHR RNGQHVAVKL
810 820 830 840 850
IDKLKFPPNK EDLLRAEVQI LEKVDHPGVV HFMQMLETTD RIFVVMEKLK
860 870 880 890 900
GDMLEMILSS EKGRLSERTT QFLVAQILEA LRYLHHLNIV HCDLKPENIL
910 920 930 940 950
LNSNSDFPQV KLCDFGFARI IGEKSFRRSV VGTPAYLAPE VLRNKGFNRS
960 970 980 990 1000
LDMWSVGVIV YVSLSGTFPF NEDEDINDQI QNAEFMYPPT PWKEISENAI
1010 1020 1030 1040 1050
EFINGLLQVK MSKRYTVTKA QSQIWMQNYT LWSDLRVLEK AVGQRFVTHE
1060 1070
SDDVRWQAYE KEHNVTPVYV
Note: No experimental confirmation available.Curated
Length:1,070
Mass (Da):120,583
Last modified:May 20, 2008 - v4
Checksum:i6A4891629C021973
GO
Isoform b1 Publication (identifier: O45818-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-533: Missing.
     534-701: LGSQADDGAS...RTGVCVYFIS → MILNILRLPH...TKRNGWSGDA
     750-755: ETGHLG → KTRPPY
     756-1070: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:220
Mass (Da):23,291
Checksum:iB28D89D665CFEFC8
GO
Isoform c1 Publication (identifier: O45818-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-197: Missing.
     198-310: DHSDDEVWTP...GTLVEVVIGS → MTDRHPESKT...GRRTYIVIGA

Note: No experimental confirmation available.Curated
Show »
Length:872
Mass (Da):98,139
Checksum:i12B2D7AFD5611AEF
GO
Isoform d (identifier: O45818-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-222: Missing.

Note: No experimental confirmation available.
Show »
Length:848
Mass (Da):95,420
Checksum:i98934EE5E84CA210
GO
Isoform e (identifier: O45818-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     470-471: Missing.

Note: No experimental confirmation available.
Show »
Length:1,068
Mass (Da):120,342
Checksum:i337D5CDC5CE2532E
GO
Isoform f (identifier: O45818-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     541-541: G → GTSLTGSMDNLRMCSVPFGSDQSTSG

Note: No experimental confirmation available.
Show »
Length:1,095
Mass (Da):123,144
Checksum:i8760C7BF376214BB
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0531501 – 533Missing in isoform b. 1 PublicationAdd BLAST533
Alternative sequenceiVSP_0532471 – 222Missing in isoform d. CuratedAdd BLAST222
Alternative sequenceiVSP_0531511 – 197Missing in isoform c. 1 PublicationAdd BLAST197
Alternative sequenceiVSP_053152198 – 310DHSDD…VVIGS → MTDRHPESKTSTSSNSPSIS SSTSSSLKMMKKQQRAKSCA TPNSGRKSPRLEVKAMTISS SPTAQRFVFQQQASFQVLED FENLKVYEEKEKQRKKEKAP DVGRRTYIVIGA in isoform c. 1 PublicationAdd BLAST113
Alternative sequenceiVSP_053248470 – 471Missing in isoform e. Curated2
Alternative sequenceiVSP_053153534 – 701LGSQA…VYFIS → MILNILRLPHADEPTTSQSV QDYPLVHFSPRRHQKFRTII SVGDSDVIKDSSLTDEELYN IIHASPIARKSSTVSSTDSG YLGSSGASSSCVRSREGSTV SSTITVERTRRGGSTASSEP DSVADSEGAGSYSSFSSIAS TASRMLGRAADCLVLMTKRN GWSGDA in isoform b. 1 PublicationAdd BLAST168
Alternative sequenceiVSP_053249541G → GTSLTGSMDNLRMCSVPFGS DQSTSG in isoform f. Curated1
Alternative sequenceiVSP_053154750 – 755ETGHLG → KTRPPY in isoform b. 1 Publication6
Alternative sequenceiVSP_053155756 – 1070Missing in isoform b. 1 PublicationAdd BLAST315

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z82052
, AL021507, AL021572, Z92967 Genomic DNA Translation: CAB04830.2
Z92967 Genomic DNA Translation: CAB07477.1
Z92967, AL021507, AL021572 Genomic DNA Translation: CAQ35035.1
Z92967, AL021507, AL021572 Genomic DNA Translation: CCD31057.1
Z82052
, AL021507, AL021572, Z92967 Genomic DNA Translation: CCG28246.1
Z82052
, AL021507, AL021572, Z92967 Genomic DNA Translation: CCG28247.1
RefSeqiNP_001123022.1, NM_001129550.1 [O45818-3]
NP_001256724.1, NM_001269795.1 [O45818-5]
NP_001256725.1, NM_001269796.1 [O45818-6]
NP_001256726.1, NM_001269797.1 [O45818-4]
NP_507239.2, NM_074838.5 [O45818-1]
NP_507240.1, NM_074839.3
UniGeneiCel.17825

Genome annotation databases

EnsemblMetazoaiT25E12.4a; T25E12.4a; WBGene00012019 [O45818-1]
GeneIDi180121
KEGGicel:CELE_T25E12.4
UCSCiT25E12.4a c. elegans

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDKF2_CAEEL
AccessioniPrimary (citable) accession number: O45818
Secondary accession number(s): B1V8I6
, G5EEQ6, H9G324, H9G325, O62166, O62167
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 20, 2008
Last modified: March 28, 2018
This is version 162 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health