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Protein

Tyrosine protein-kinase src-2

Gene

src-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Non-receptor tyrosine-protein kinase which may play a role in larval and pharynx development. Unlike src-1, does not play a role in embryonic development.1 Publication

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.1 Publication

Cofactori

Mg2+By similarity, Mn2+By similarity

Enzyme regulationi

May be inhibited by csk-1-mediated phosphorylation at Tyr-500.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei268 – 2681ATPPROSITE-ProRule annotation
Active sitei358 – 3581Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2549ATPPROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

  • pharynx development Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO45539.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine protein-kinase src-2Curated (EC:2.7.10.21 Publication)
Alternative name(s):
SRC oncogene related protein 2Imported
Gene namesi
Name:src-2Imported
Synonyms:kin-22Imported
ORF Names:F49B2.5Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiF49B2.5; CE24991; WBGene00005078; src-2.

Pathology & Biotechi

Disruption phenotypei

RNAi-mediated knockdown causes no obvious effect on embryonic development (PubMed:12527374). Animals have a slight decrease in unc-5 tyrosine phosphorylation (PubMed:16024786).2 Publications

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi268 – 2681K → M: Probable loss of kinase activity. In a wild type background, 30 percent die at the larval stage and 4 percent of surviving animals have abnormalities in the pharynx structure. 1 Publication
Mutagenesisi500 – 5001Y → F: Abolishes phosphorylation which results in constitutive activation. In a wild type background, 76 percent die at the larval stage and 34 percent of surviving animals have abnormalities in the pharynx structure. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 507506Tyrosine protein-kinase src-2CuratedPRO_0000434508Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Modified residuei500 – 5001Phosphotyrosine1 Publication

Post-translational modificationi

May be phosphorylated on Tyr-500 by csk-1.1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO45539.

Expressioni

Tissue specificityi

Expressed in vulva, cells around anus and pharyngeal muscles.1 Publication

Gene expression databases

ExpressionAtlasiO45539. baseline and differential.

Interactioni

Protein-protein interaction databases

DIPiDIP-26743N.
IntActiO45539. 43 interactions.
MINTiMINT-117088.
STRINGi6239.F49B2.5.

Structurei

3D structure databases

ProteinModelPortaliO45539.
SMRiO45539. Positions 62-501.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini57 – 11862SH3PROSITE-ProRule annotationAdd
BLAST
Domaini124 – 21693SH2PROSITE-ProRule annotationAdd
BLAST
Domaini240 – 494255Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation
Contains 1 SH3 domain.PROSITE-ProRule annotation

Keywords - Domaini

SH2 domain, SH3 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
InParanoidiO45539.
KOiK08892.
OMAiFTMDPSE.
OrthoDBiEOG7GTT2V.
PhylomeDBiO45539.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O45539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSCIGKEDP PPGATSPVHT SSTLGRESLP SHPRIPSIGP IAASSSGNTI
60 70 80 90 100
DKNQNISQSA NFVALFQYDA RTDDDLSFKK DDILEILNDT QGDWWFARHK
110 120 130 140 150
ATGRTGYIPS NYVAREKSIE SQPWYFGKMR RIDAEKCLLH TLNEHGAFLV
160 170 180 190 200
RDSESRQHDL SLSVRENDSV KHYRIRQLDH GGYFIARRRP FATLHDLIAH
210 220 230 240 250
YQREADGLCV NLGAPCAKSE APQTTTFTYD DQWEVDRRSV RLIRQIGAGQ
260 270 280 290 300
FGEVWEGRWN VNVPVAVKKL KAGTADPTDF LAEAQIMKKL RHPKLLSLYA
310 320 330 340 350
VCTRDEPILI VTELMQENLL TFLQRRGRQC QMPQLVEISA QVAAGMAYLE
360 370 380 390 400
EMNFIHRDLA ARNILINNSL SVKIADFGLA RILMKENEYE ARTGARFPIK
410 420 430 440 450
WTAPEAANYN RFTTKSDVWS FGILLTEIVT FGRLPYPGMT NAEVLQQVDA
460 470 480 490 500
GYRMPCPAGC PVTLYDIMQQ CWRSDPDKRP TFETLQWKLE DLFNLDSSEY

KEASINF
Length:507
Mass (Da):57,530
Last modified:May 1, 2000 - v2
Checksum:iCD3DC68FC91ED86D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CAB04427.2.
PIRiT22405.
RefSeqiNP_493502.1. NM_061101.4.
UniGeneiCel.18455.

Genome annotation databases

EnsemblMetazoaiF49B2.5; F49B2.5; WBGene00005078.
GeneIDi173296.
KEGGicel:CELE_F49B2.5.
UCSCiF49B2.5. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BX284601 Genomic DNA. Translation: CAB04427.2.
PIRiT22405.
RefSeqiNP_493502.1. NM_061101.4.
UniGeneiCel.18455.

3D structure databases

ProteinModelPortaliO45539.
SMRiO45539. Positions 62-501.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-26743N.
IntActiO45539. 43 interactions.
MINTiMINT-117088.
STRINGi6239.F49B2.5.

Proteomic databases

PaxDbiO45539.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF49B2.5; F49B2.5; WBGene00005078.
GeneIDi173296.
KEGGicel:CELE_F49B2.5.
UCSCiF49B2.5. c. elegans.

Organism-specific databases

CTDi173296.
WormBaseiF49B2.5; CE24991; WBGene00005078; src-2.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
GeneTreeiENSGT00760000118938.
HOGENOMiHOG000233858.
InParanoidiO45539.
KOiK08892.
OMAiFTMDPSE.
OrthoDBiEOG7GTT2V.
PhylomeDBiO45539.

Enzyme and pathway databases

SignaLinkiO45539.

Miscellaneous databases

NextBioi879039.

Gene expression databases

ExpressionAtlasiO45539. baseline and differential.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR001452. SH3_domain.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 1 hit.
PF00017. SH2. 1 hit.
PF00018. SH3_1. 1 hit.
[Graphical view]
PRINTSiPR00401. SH2DOMAIN.
PR00452. SH3DOMAIN.
PR00109. TYRKINASE.
SMARTiSM00252. SH2. 1 hit.
SM00326. SH3. 1 hit.
SM00219. TyrKc. 1 hit.
[Graphical view]
SUPFAMiSSF50044. SSF50044. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
PS50002. SH3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Distinct roles of the Src family kinases, SRC-1 and KIN-22, that are negatively regulated by CSK-1 in C. elegans."
    Hirose T., Koga M., Ohshima Y., Okada M.
    FEBS Lett. 534:133-138(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY, ENZYME REGULATION, TISSUE SPECIFICITY, PHOSPHORYLATION, DISRUPTION PHENOTYPE, MUTAGENESIS OF LYS-268 AND TYR-500.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2Imported.
  3. "SRC-1 mediates UNC-5 signaling in Caenorhabditis elegans."
    Lee J., Li W., Guan K.L.
    Mol. Cell. Biol. 25:6485-6495(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiSRC2_CAEEL
AccessioniPrimary (citable) accession number: O45539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 14, 2015
Last sequence update: May 1, 2000
Last modified: May 11, 2016
This is version 137 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.