Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O45293

- GALT8_CAEEL

UniProt

O45293 - GALT8_CAEEL

Protein

Probable N-acetylgalactosaminyltransferase 8

Gene

gly-8

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Potential glycopeptide transferase involved in O-linked oligosaccharide biosynthesis By similarity. In contrast to other members of the family, it does not act as a peptide transferase that transfers GalNAc onto serine or threonine residue on peptides that have been tested. Some peptide transferase activity is however not excluded, considering that its appropriate peptide substrate may remain unidentified.By similarity

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei147 – 1471SubstrateBy similarity
    Binding sitei180 – 1801SubstrateBy similarity
    Metal bindingi203 – 2031ManganeseBy similarity
    Binding sitei204 – 2041SubstrateBy similarity
    Metal bindingi205 – 2051ManganeseBy similarity
    Binding sitei308 – 3081SubstrateBy similarity
    Metal bindingi336 – 3361ManganeseBy similarity
    Binding sitei339 – 3391SubstrateBy similarity
    Binding sitei344 – 3441SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. transferase activity, transferring glycosyl groups Source: UniProtKB-KW

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT27. Glycosyltransferase Family 27.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable N-acetylgalactosaminyltransferase 8 (EC:2.4.1.-)
    Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 8
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 8
    Short name:
    pp-GaNTase 8
    Gene namesi
    Name:gly-8
    ORF Names:Y66A7A.6
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome III

    Organism-specific databases

    WormBaseiY66A7A.6; CE15691; WBGene00001633; gly-8.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 421421Probable N-acetylgalactosaminyltransferase 8PRO_0000059151Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi52 – 521N-linked (GlcNAc...)1 Publication
    Glycosylationi58 – 581N-linked (GlcNAc...)1 Publication
    Disulfide bondi98 ↔ 331By similarity
    Disulfide bondi322 ↔ 399By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    PaxDbiO45293.

    Interactioni

    Protein-protein interaction databases

    BioGridi41776. 2 interactions.
    DIPiDIP-24790N.
    MINTiMINT-1070872.
    STRINGi6239.Y66A7A.6.1.

    Structurei

    3D structure databases

    ProteinModelPortaliO45293.
    SMRiO45293. Positions 76-421.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 33CytoplasmicSequence Analysis
    Topological domaini25 – 421397LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei4 – 2421Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni106 – 219114Catalytic subdomain AAdd
    BLAST
    Regioni277 – 33963Catalytic subdomain BAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi418 – 4214Prevents secretion from ERPROSITE-ProRule annotation

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG295988.
    GeneTreeiENSGT00670000097647.
    HOGENOMiHOG000038227.
    InParanoidiO45293.
    OMAiPENNVKP.
    PhylomeDBiO45293.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF53448. SSF53448. 1 hit.
    PROSITEiPS00014. ER_TARGET. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O45293-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRRHVVLSIF VFAGIVFAAE EAEKLPKCEH VDPYENLEGW LDLKPLTERK    50
    CNHTLKENLT EAESKKSEWG IKSFAFDALS SEKLGPNRNV GKQAHKLCEE 100
    EKYDASYSTS VVVIHHNEAL STILRMINGI IEFTPKSLLK EIVLYEDASE 150
    EDHVLTKHLE KFAKIKGLED KLIIKRSEYR QGLIRAKVHA SRLATGEVIV 200
    FMDSHCEVAE RWLEPLLQPI KEDPKSIVLP VVDLINPVSF DYSPSMVAKS 250
    GFDWGFTFKW IYLPWEYFET PENNVKPFNS PAMPGGLLAM RKEYFVELGE 300
    YDMGMEIWGS ENIELSLKAW LCGGRVVVAP CSRVGHVFRM RRPYTSKPGM 350
    DTALYNAVRV AKTWLGEYES KFFAVKPRGA KMVFGDLTEP MQVKDRLKCK 400
    DMKWFIENVY PELEPKVHDE L 421
    Length:421
    Mass (Da):48,358
    Last modified:June 1, 1998 - v1
    Checksum:i3FA38E18702D087D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031842 mRNA. Translation: AAC13678.1.
    AL590342 Genomic DNA. Translation: CAC35860.1.
    PIRiT42252.
    RefSeqiNP_499504.1. NM_067103.4.
    UniGeneiCel.19663.

    Genome annotation databases

    EnsemblMetazoaiY66A7A.6.1; Y66A7A.6.1; WBGene00001633.
    Y66A7A.6.2; Y66A7A.6.2; WBGene00001633.
    GeneIDi176595.
    KEGGicel:CELE_Y66A7A.6.
    UCSCiY66A7A.6.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031842 mRNA. Translation: AAC13678.1 .
    AL590342 Genomic DNA. Translation: CAC35860.1 .
    PIRi T42252.
    RefSeqi NP_499504.1. NM_067103.4.
    UniGenei Cel.19663.

    3D structure databases

    ProteinModelPortali O45293.
    SMRi O45293. Positions 76-421.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 41776. 2 interactions.
    DIPi DIP-24790N.
    MINTi MINT-1070872.
    STRINGi 6239.Y66A7A.6.1.

    Protein family/group databases

    CAZyi GT27. Glycosyltransferase Family 27.

    Proteomic databases

    PaxDbi O45293.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai Y66A7A.6.1 ; Y66A7A.6.1 ; WBGene00001633 .
    Y66A7A.6.2 ; Y66A7A.6.2 ; WBGene00001633 .
    GeneIDi 176595.
    KEGGi cel:CELE_Y66A7A.6.
    UCSCi Y66A7A.6.1. c. elegans.

    Organism-specific databases

    CTDi 176595.
    WormBasei Y66A7A.6 ; CE15691 ; WBGene00001633 ; gly-8.

    Phylogenomic databases

    eggNOGi NOG295988.
    GeneTreei ENSGT00670000097647.
    HOGENOMi HOG000038227.
    InParanoidi O45293.
    OMAi PENNVKP.
    PhylomeDBi O45293.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Miscellaneous databases

    NextBioi 893222.
    PROi O45293.

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53448. SSF53448. 1 hit.
    PROSITEi PS00014. ER_TARGET. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "cDNA cloning and expression of a family of UDP-N-acetyl-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase sequence homologs from Caenorhabditis elegans."
      Hagen F.K., Nehrke K.
      J. Biol. Chem. 273:8268-8277(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Proteomics reveals N-linked glycoprotein diversity in Caenorhabditis elegans and suggests an atypical translocation mechanism for integral membrane proteins."
      Kaji H., Kamiie J., Kawakami H., Kido K., Yamauchi Y., Shinkawa T., Taoka M., Takahashi N., Isobe T.
      Mol. Cell. Proteomics 6:2100-2109(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-52 AND ASN-58, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: Bristol N2.

    Entry informationi

    Entry nameiGALT8_CAEEL
    AccessioniPrimary (citable) accession number: O45293
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: August 16, 2004
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Caution

    In contrast to other members of the family, it lacks the C-terminal ricin B-type lectin domain, which usually contributes to the glycopeptide specificity. It instead contains a motif that may retain its secretion form the endoplasmic reticulum.Curated

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3