O45145 (ACASE_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alkaline ceramidase
Short name=AlkCDase
EC=3.5.1.23

Alternative name(s):
Alkaline N-acylsphingosine amidohydrolase
Alkaline acylsphingosine deacylase

Gene names

ORF Names:

W02F12.2

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Alkaline ceramidase		PRO_0000247751
Regions
	Transmembrane	34 – 54	21	Helical; Potential
	Transmembrane	61 – 81	21	Helical; Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Transmembrane	124 – 144	21	Helical; Potential
	Transmembrane	148 – 168	21	Helical; Potential
	Transmembrane	183 – 203	21	Helical; Potential
	Transmembrane	214 – 234	21	Helical; Potential
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O45145 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: B9E5357A054D10C4	FASTA	272	31,162
	10 20 30 40 50 60 MESSSINRWF EYESGHAWCE SAYKYQTLPY VAEFANTCTN LPIIVLPLVN IMLLRRYLHD 70 80 90 100 110 120 VNGGLIFPQL LLTFNGLAST YYHATLNLFG QLVDELSLVW IITVFLVVYI PVMKWFPEKF 130 140 150 160 170 180 SKRLTLVRWV VLIVTALVSG LCFLEPNLNA IALMLFSIPA AVVINYEGKQ SGIPDIESFP 190 200 210 220 230 240 SRILALWGVA FSFWFADRLL CDFWLYLGTP YLHALFHLLA GLAGYTIFIM FSMIDIESRT 250 260 270 KTHKYTAAVR YFPGKNGSIF SFPYISLKER SQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FO081630 Genomic DNA. Translation: CCD72931.1.
PIR	T32993.
RefSeq	NP_504697.2. NM_072296.4.
UniGene	Cel.2046.
3D structure databases
ProteinModelPortal	O45145.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	W02F12.2; W02F12.2; W02F12.2.
GeneID	189124.
KEGG	cel:W02F12.2.
NMPDR	fig|6239.3.peg.18220.
UCSC	W02F12.2. c. elegans.
Organism-specific databases
CTD	189124.
WormBase	W02F12.2; CE33333; WBGene00020947.
Phylogenomic databases
eggNOG	meNOG05933.
GeneTree	EMGT00050000016840.
HOGENOM	HBG386102.
InParanoid	O45145.
OMA	KRCDNVR.
PhylomeDB	O45145.
Gene expression databases
ArrayExpress	O45145.
Family and domain databases
InterPro	IPR008901. Ceramidase. [Graphical view]
Pfam	PF05875. Ceramidase. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	941292.

Entry information

Entry name

ACASE_CAEEL

Accession

Primary (citable) accession number: O45145

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2003
Last modified:	December 14, 2011
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project