Reviewed, UniProtKB/Swiss-Prot O45145 (ACASE_CAEEL)

Names and origin

Protein names

Recommended name:
    Alkaline ceramidase
      Short name=AlkCDase
    EC=3.5.1.23
Alternative name(s):
    Alkaline N-acylsphingosine amidohydrolase
    Alkaline acylsphingosine deacylase

Gene names

ORF Names:

W02F12.2

Organism

Caenorhabditis elegans [Complete proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	272 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid By similarity.
Catalytic activity	N-acylsphingosine + H2O = a carboxylate + sphingosine.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the alkaline ceramidase family.

Ontologies

Keywords
Biological process	Lipid metabolism
Cellular component	Membrane
Domain	Transmembrane
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ceramide metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	Golgi membrane Inferred from electronic annotation. Source: InterPro endoplasmic reticulum membrane Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ceramidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 272	272	Alkaline ceramidase		PRO_0000247751
Regions
	Transmembrane	34 – 54	21	Potential
	Transmembrane	61 – 81	21	Potential
	Transmembrane	96 – 116	21	Potential
	Transmembrane	124 – 144	21	Potential
	Transmembrane	148 – 168	21	Potential
	Transmembrane	183 – 203	21	Potential
	Transmembrane	214 – 234	21	Potential
Amino acid modifications
	Glycosylation	256	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O45145-1 [UniParc]. Last modified June 1, 2003. Version 2. Checksum: B9E5357A054D10C4	FASTA	272	31,162
	10 20 30 40 50 60 MESSSINRWF EYESGHAWCE SAYKYQTLPY VAEFANTCTN LPIIVLPLVN IMLLRRYLHD 70 80 90 100 110 120 VNGGLIFPQL LLTFNGLAST YYHATLNLFG QLVDELSLVW IITVFLVVYI PVMKWFPEKF 130 140 150 160 170 180 SKRLTLVRWV VLIVTALVSG LCFLEPNLNA IALMLFSIPA AVVINYEGKQ SGIPDIESFP 190 200 210 220 230 240 SRILALWGVA FSFWFADRLL CDFWLYLGTP YLHALFHLLA GLAGYTIFIM FSMIDIESRT 250 260 270 KTHKYTAAVR YFPGKNGSIF SFPYISLKER SQ

References

[1]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047653 Genomic DNA. Translation: AAC04458.2.
PIR	T32993.
RefSeq	NP_504697.2.
UniGene	Cel.2046
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	W02F12.2; W02F12.2; W02F12.2; Caenorhabditis elegans. [Genome view]
GeneID	189124.
KEGG	cel:W02F12.2.
NMPDR	fig|6239.3.peg.18220.
UCSC	W02F12.2. c. elegans.
Organism-specific databases
CTD	189124.
WormBase	WBGene00020947. W02F12.2.
WormPep	W02F12.2. CE33333. [WorfDB]
Phylogenomic databases
OMA	FQYSELV.
Enzyme and pathway databases
BRENDA	3.5.1.23. 672.
Gene expression databases
ArrayExpress	O45145.
Family and domain databases
InterPro	IPR008901. APHC. [Graphical view]
Pfam	PF05875. aPHC. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	941292.

Entry information

Entry name

ACASE_CAEEL

Accession

Primary (citable) accession number: O45145

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 25, 2006
Last sequence update:	June 1, 2003
Last modified:	November 3, 2009
This is version 51 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project