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Protein

Death-associated protein kinase dapk-1

Gene

dapk-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Negative regulator of epidermal barrier repair and innate immune responses to wounding (PubMed:19164535, PubMed:27661253). The role in epidermal tissue integrity and wound healing is established through the inhibition of epidermal microtubule stability, possibly via the negative regulation of the microtubule minus-end binding protein ptrn-1 (PubMed:27661253). Appears to act downstream of or in parallel to muscarinic signaling in the regulation of autophagy (PubMed:17785524).3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Mg2+By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei57ATPPROSITE-ProRule annotationBy similarity1
Active sitei155Proton acceptorPROSITE-ProRule annotationBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi34 – 42ATPPROSITE-ProRule annotationBy similarity9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein, Kinase, Serine/threonine-protein kinase, Transferase
Biological processImmunity, Innate immunity
LigandATP-binding, GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO44997

Names & Taxonomyi

Protein namesi
Recommended name:
Death-associated protein kinase dapk-1 (EC:2.7.11.1)
Gene namesi
Name:dapk-1Imported
Synonyms:mor-3, tag-119
ORF Names:K12C11.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome I

Organism-specific databases

WormBaseiK12C11.4 ; CE40262 ; WBGene00003400 ; dapk-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

Pathology & Biotechi

Disruption phenotypei

Beginning in the L3 stage, animals display striking and progressive defects in morphology of the epidermis and cuticle in several body regions, particularly in the nose, tail, vulva, and the dorsal midline in the region of the posterior pharyngeal bulb (PubMed:19164535, PubMed:27661253). The cuticle in these regions becomes up to 5-10 times thicker than the wild-type, at the expense of underlying epidermis (PubMed:19164535, PubMed:27661253). Up-regulates innate immune responses to damage (PubMed:19164535). Decreases starvation-induced autophagy (PubMed:17785524). Disorganization and hyper-stabilization of epidermal microtubules and reduced microtubule growth rates (PubMed:27661253). Treatment with a microtubule stabilizing drug, paclitaxel, results in enhanced epidermal morphology defects (PubMed:27661253). In a ptrn-1 mutant background, suppression of the epidermal morphology defects (PubMed:27661253).3 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi57K → A: Normal localization and movement along microtubules and no exhibition of epidermal morphology defects. 1 Publication1
Mutagenesisi179S → L in ju4; putative gain of function mutation. Leads to highly penetrant epidermal morphology defects. Progressive accumulation of the cuticle and degeneration of the underlying epidermis at the extreme anterior and posterior as well as the dorsal midline. Accelerated wound closure. Disorganization and hyper-stabilization of epidermal microtubules. Reduced microtubule growth rates. Elevated expression of antimicrobial peptides. Increased ptrn-1 filaments in the anterior lateral epidermis. In a ptrn-1 mutant background, suppression of the epidermal defects, the elevated antimicrobial peptide expression, the accelerated wound closure and the microtubule architecture defects. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004000891 – 1425Death-associated protein kinase dapk-1Add BLAST1425

Proteomic databases

EPDiO44997
PaxDbiO44997
PeptideAtlasiO44997
PRIDEiO44997

Expressioni

Tissue specificityi

Expressed in epidermis, muscles and neurons.1 Publication

Developmental stagei

Present from late embryogenesis onwards.1 Publication

Gene expression databases

BgeeiWBGene00003400
ExpressionAtlasiO44997 baseline and differential

Interactioni

Subunit structurei

Interacts with ptrn-1.1 Publication

Protein-protein interaction databases

STRINGi6239.K12C11.4

Structurei

3D structure databases

ProteinModelPortaliO44997
SMRiO44997
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini28 – 289Protein kinasePROSITE-ProRule annotationAdd BLAST262
Repeati392 – 421ANK 1Sequence analysisAdd BLAST30
Repeati425 – 454ANK 2Sequence analysisAdd BLAST30
Repeati458 – 487ANK 3Sequence analysisAdd BLAST30
Repeati491 – 520ANK 4Sequence analysisAdd BLAST30
Repeati524 – 553ANK 5Sequence analysisAdd BLAST30
Repeati557 – 586ANK 6Sequence analysisAdd BLAST30
Repeati590 – 619ANK 7Sequence analysisAdd BLAST30
Repeati623 – 652ANK 8Sequence analysisAdd BLAST30
Domaini695 – 950RocPROSITE-ProRule annotationAdd BLAST256
Repeati810 – 841ANK 9Sequence analysisAdd BLAST32
Repeati934 – 963ANK 10Sequence analysisAdd BLAST30
Domaini1308 – 1389DeathPROSITE-ProRule annotationAdd BLAST82

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat

Phylogenomic databases

eggNOGiKOG0032 Eukaryota
ENOG410XRMJ LUCA
GeneTreeiENSGT00760000118877
HOGENOMiHOG000082489
InParanoidiO44997
KOiK08803
OMAiCQMEVIK
OrthoDBiEOG091G0J2O
PhylomeDBiO44997

Family and domain databases

CDDicd00204 ANK, 3 hits
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR020676 DAPK1
IPR011029 DEATH-like_dom_sf
IPR000488 Death_domain
IPR011009 Kinase-like_dom_sf
IPR027417 P-loop_NTPase
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR020859 ROC_dom
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR44619 PTHR44619, 1 hit
PfamiView protein in Pfam
PF12796 Ank_2, 2 hits
PF00531 Death, 1 hit
PF00069 Pkinase, 1 hit
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 9 hits
SM00005 DEATH, 1 hit
SM00220 S_TKc, 1 hit
SUPFAMiSSF47986 SSF47986, 1 hit
SSF48403 SSF48403, 1 hit
SSF52540 SSF52540, 2 hits
SSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 7 hits
PS50017 DEATH_DOMAIN, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit
PS51424 ROC, 1 hit

Sequencei

Sequence statusi: Complete.

O44997-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSDDVNSSAT STSSSTVHFD DTPFEDVYEI ETELGSGQFA VVRRVRDRKT
60 70 80 90 100
GEKYAAKFIK KRRYATSRRG VTRQNIEREV RVLQKIRGNS NVVELHAVYE
110 120 130 140 150
TASDVIIVLE LVSGGELFDH VCAKECLDEV EAAAFIKQIL LAVRHLHSLH
160 170 180 190 200
IVHLDIKPEN VMLKQRGDSQ IKIIDFGLSR EIEPGAVVKD MVGTPEFVAP
210 220 230 240 250
EVVNYEALSP ATDMWAVGVV TYILLSGGSP FLGDNRDETF SNITRVRYHF
260 270 280 290 300
SDRYFKNTSK HAKDFIYRLF VRDVDQRATV EECLQHPWIR GPEGNAIDIR
310 320 330 340 350
KASCITISHI QSFKTRQRWK RCVELVMVLL KASKSSRRIG DGRFDEEDMV
360 370 380 390 400
ASCTLICAEE GNLRALHKLS ALHKLLPNAT RKSLKSSFSE PNGATAMHCA
410 420 430 440 450
AKYGHAEVFN YFHMKGGNIC ARDDNGDTPL HVACRFAQHT VAGYVANEKI
460 470 480 490 500
DVDSINKTGE TALHCAVESA DTRVVRLLLQ LRPRLDLPNA SGDTVLHLAA
510 520 530 540 550
DSINPRIVPL LVCLAPPLHL RNIREETPLH VAAARGHVDC VQALLDANSP
560 570 580 590 600
IDAVEQDGKT ALIIALENGN VDIASILITN GCDINHADHH GDTALHIASK
610 620 630 640 650
HGLLQAVQTL CHCAVTVDSV NANKKTALHL AAHYGHVDII RVLLLARADV
660 670 680 690 700
TLRGDDGLTA ELVAVAAERL EAHSLLKMVK SQEIREEYIS QLYPLDTSLR
710 720 730 740 750
RIKLKLLGHS QSGKTRLVQT LHSSRGISSF LESVTRRISD HYSPSSSMKD
760 770 780 790 800
DGIHSTNGSF VSESNNNSSF DLAAAAGSKY APPHSQYTRG IDVQTVNING
810 820 830 840 850
CGEFSVWEFG GYEPMHTCYD HFVGNADCIH LILYRTSDPT EVQYKQILYW
860 870 880 890 900
MNFLKGRVTP FEPIGHCGFS SRRSKVIIVG THATSSLFPQ MNQEGEYVSS
910 920 930 940 950
DIEAMLNTVR LRFETHFDMD HRLILLDATN PSCIGMKTLK MELAKCRTNI
960 970 980 990 1000
LAKLLKPLAI LDTVVNHLNL VRKKHANFPV ITWPDFIQLV RNEINPLTGD
1010 1020 1030 1040 1050
AHCRQIVQQL QLIGELVYLR NDLCDADYVV LNAEWFGTHI LGQLLSAEFL
1060 1070 1080 1090 1100
SKASPNGSYH TSSLAKIFPE IPEQSDLMTI LEVLQLCAPD ARTGAHEFPV
1110 1120 1130 1140 1150
FIQTEAPDSI WRPYSLKEKE RDTVYGGVRI LPMRGMERSL HSTFPRIQVA
1160 1170 1180 1190 1200
LRRSINDYQP AKDTQLHQWS ECSKLVSQDR EAVIRMVGDA VEIRARGPSE
1210 1220 1230 1240 1250
SATSMFYFME DLINLVEHAA AEVGPGISLE RHFISPKHLK EHREHPALFP
1260 1270 1280 1290 1300
PESMMEMQQR ESLSVKGTQD EEELFTDVVC FGSRDVARHL TLGIDVGVAD
1310 1320 1330 1340 1350
LQMASRCELA CLLDPPHAMG RDWSILAVKL QLTDQVPDVD STGQSLSRTD
1360 1370 1380 1390 1400
QLLNEWAIHH PEQASVGNLC RILVELGRCD ARDALYRTVP LYVFAPLEDQ
1410 1420
FLLETNDSGV VSSCHSSSEH NPINI
Length:1,425
Mass (Da):158,968
Last modified:July 11, 2006 - v2
Checksum:i1118403F098DAFC5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY733040 mRNA Translation: AAW57534.1
FO081631 Genomic DNA Translation: CCD72943.1
PIRiT32930
RefSeqiNP_490840.2, NM_058439.4
UniGeneiCel.18457

Genome annotation databases

EnsemblMetazoaiK12C11.4a; K12C11.4a; WBGene00003400
GeneIDi187322
KEGGicel:CELE_K12C11.4
UCSCiK12C11.4 c. elegans

Similar proteinsi

Entry informationi

Entry nameiDAPK_CAEEL
AccessioniPrimary (citable) accession number: O44997
Secondary accession number(s): Q3ZLV1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: July 11, 2006
Last modified: May 23, 2018
This is version 160 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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