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Protein

Neprilysin-2

Gene

nep-2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for olfactory plasticity, which is the change from positive chemotaxis to dispersal after prolonged exposure to an odorant. Thought to antagonise snet-1 by degrading excess snet-1 peptides and thus enabling olfactory plasticity.1 Publication1 Publication

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi571Zinc; catalyticBy similarity1
Active sitei572By similarity1
Metal bindingi575Zinc; catalyticBy similarity1
Metal bindingi633Zinc; catalyticBy similarity1
Active sitei637Proton donorBy similarity1

GO - Molecular functioni

  • metalloendopeptidase activity Source: InterPro
  • metallopeptidase activity Source: UniProtKB
  • zinc ion binding Source: UniProtKB

GO - Biological processi

  • olfactory learning Source: UniProtKB
  • regulation of chemotaxis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Metalloprotease, Protease

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-CEL-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiM13.015.

Names & Taxonomyi

Protein namesi
Recommended name:
Neprilysin-2Imported (EC:3.4.24.-Curated)
Gene namesi
Name:nep-2Imported
ORF Names:T05A8.4Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome II

Organism-specific databases

WormBaseiT05A8.4; CE30162; WBGene00020230; nep-2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 19CytoplasmicAdd BLAST19
Transmembranei20 – 40Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini41 – 736ExtracellularAdd BLAST696

GO - Cellular componenti

  • cell surface Source: UniProtKB
  • integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Severe defect in olfactory plasticity in response to the odorants benzaldehyde, isoamylalcohol, diacetyl and pyrazine.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi571H → F: Fails to rescue the nep-2 olfactory plasticity defect, suggesting that peptidase activity is required for olfactory plasticity; when associated with F-575. 1 Publication1
Mutagenesisi575H → F: Fails to rescue the nep-2 olfactory plasticity defect, suggesting that peptidase activity is required for olfactory plasticity; when associated with F-571. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004314191 – 736Neprilysin-2Add BLAST736

Proteomic databases

EPDiO44857.
PaxDbiO44857.
PeptideAtlasiO44857.
PRIDEiO44857.

Expressioni

Tissue specificityi

Expressed in muscle cells, GLR cells, SMB motor neurons and AIM interneurons.1 Publication

Gene expression databases

BgeeiWBGene00020230.

Interactioni

Protein-protein interaction databases

DIPiDIP-24357N.
MINTiMINT-1120207.
STRINGi6239.T05A8.4.

Structurei

3D structure databases

ProteinModelPortaliO44857.
SMRiO44857.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili103 – 123Sequence analysisAdd BLAST21

Sequence similaritiesi

Belongs to the peptidase M13 family.Sequence analysis

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
InParanoidiO44857.
KOiK08635.
OMAiINMQING.
OrthoDBiEOG091G025Y.
PhylomeDBiO44857.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR031122. Nep-2.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF137. PTHR11733:SF137. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O44857-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPDEEDGTT KSPGSRWTRI WAIIALILLI LFLLVLGAAI YFYINYKDSS
60 70 80 90 100
DVCLSPGCIK TASVILSSMN SSVDPCDDFY EFACGQWIKG HPIPDDAPSV
110 120 130 140 150
SNFENLGQDL EFALKELLDE NDEPYDYETS AVGKAKYFYN LCLNESEILD
160 170 180 190 200
NWRTTFDEVV KSFGGWPSLG HQMKPDASIE MLYADMVAKF KADSLFKATV
210 220 230 240 250
QPDDKNSQRH VLLIDQPQLN LFARDFYVAA ENEERMAYLQ LIRDVLILLD
260 270 280 290 300
ADRTRATLDA KEIIDFETAL ANITMADEHR HDIAELYTKI TLGEMRRSLP
310 320 330 340 350
HFNWPLFFNR MFKDLHEKNG KRITFDDNTE VVVYGYEFLR RLDVLIPQYD
360 370 380 390 400
NRLIVNYLEW CWFFKTMLRD LPDPFALTIF KFYKTLNIMN VQKVRWHGCV
410 420 430 440 450
TRINSLMPMA TSAIYVKNHF DHEAKQQVEE MISLIMESFV DLLLSEDWLT
460 470 480 490 500
KETKQTAKQK VNEMKRKIGY PDYLNDPAAV NNEYKTFKVY PGHYYQTKFS
510 520 530 540 550
FYEQYQRDVL ERITEAVDRE RWVAGAALVN AFYSPNTNEI IFPAGILQPV
560 570 580 590 600
FYSKDFPSSM NFGGIGVVIG HEITHGFDDR GRLYDNLGNI RQWWDNATIS
610 620 630 640 650
KFEHKAQCIE KQYSSYVLDQ INMQINGKST KGENIADNGG LKQAYRAYKK
660 670 680 690 700
YEKRHSRPPR LPGVNLTHDQ LFFLNYAQIW CGTMNDKEAI RKLRTSEHSP
710 720 730
GPIRVKGPLS NSYDFAKAYN CEPGSQMNPR EKCRVW
Length:736
Mass (Da):85,422
Last modified:March 1, 2002 - v2
Checksum:i5B55DD364EF2F2B9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081704 Genomic DNA. Translation: CCD73586.1.
RefSeqiNP_494343.2. NM_061942.2.
UniGeneiCel.20256.

Genome annotation databases

EnsemblMetazoaiT05A8.4; T05A8.4; WBGene00020230.
GeneIDi188090.
KEGGicel:CELE_T05A8.4.
UCSCiT05A8.4. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081704 Genomic DNA. Translation: CCD73586.1.
RefSeqiNP_494343.2. NM_061942.2.
UniGeneiCel.20256.

3D structure databases

ProteinModelPortaliO44857.
SMRiO44857.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-24357N.
MINTiMINT-1120207.
STRINGi6239.T05A8.4.

Protein family/group databases

MEROPSiM13.015.

Proteomic databases

EPDiO44857.
PaxDbiO44857.
PeptideAtlasiO44857.
PRIDEiO44857.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiT05A8.4; T05A8.4; WBGene00020230.
GeneIDi188090.
KEGGicel:CELE_T05A8.4.
UCSCiT05A8.4. c. elegans.

Organism-specific databases

CTDi188090.
WormBaseiT05A8.4; CE30162; WBGene00020230; nep-2.

Phylogenomic databases

eggNOGiKOG3624. Eukaryota.
COG3590. LUCA.
GeneTreeiENSGT00760000119162.
HOGENOMiHOG000245574.
InParanoidiO44857.
KOiK08635.
OMAiINMQING.
OrthoDBiEOG091G025Y.
PhylomeDBiO44857.

Enzyme and pathway databases

ReactomeiR-CEL-6798695. Neutrophil degranulation.

Miscellaneous databases

PROiO44857.

Gene expression databases

BgeeiWBGene00020230.

Family and domain databases

CDDicd08662. M13. 1 hit.
Gene3Di3.40.390.10. 2 hits.
InterProiIPR024079. MetalloPept_cat_dom.
IPR031122. Nep-2.
IPR000718. Peptidase_M13.
IPR018497. Peptidase_M13_C.
IPR008753. Peptidase_M13_N.
[Graphical view]
PANTHERiPTHR11733. PTHR11733. 1 hit.
PTHR11733:SF137. PTHR11733:SF137. 1 hit.
PfamiPF01431. Peptidase_M13. 1 hit.
PF05649. Peptidase_M13_N. 1 hit.
[Graphical view]
PRINTSiPR00786. NEPRILYSIN.
PROSITEiPS00142. ZINC_PROTEASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNEPL2_CAEEL
AccessioniPrimary (citable) accession number: O44857
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 7, 2015
Last sequence update: March 1, 2002
Last modified: November 30, 2016
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.