O44757 (LIN59_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable histone-lysine N-methyltransferase lin-59
EC=2.1.1.43

Alternative name(s):
Abnormal cell lineage protein 59

Gene names

Name:	lin-59
ORF Names:	T12F5.4

Organism

Caenorhabditis elegans [Reference proteome]

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1312 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probable histone methyltransferase By similarity. Essential protein required to maintain expression of homeotic genes egl-5 and mab-5. May play an analogous role to the trithorax Group (trxG) proteins. TrxG proteins form multiprotein complexes that are required to maintain the transcriptionally active state of homeotic genes throughout development. May act via a modification of chromatin. Ref.1
Catalytic activity	S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone].
Subcellular location	Nucleus Probable.
Tissue specificity	Widely expressed throughout embryonic development and into adulthood. Ref.1
Developmental stage	Expressed from mid-blastula.
Sequence similarities	Belongs to the histone-lysine methyltransferase family. SET2 subfamily. Contains 1 AWS domain. Contains 1 BAH domain. Contains 1 PHD-type zinc finger. Contains 1 SET domain.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Domain	Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Activator Chromatin regulator Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ectodermal cell fate specification Inferred from mutant phenotype PubMed 10511553. Source: WormBase embryo development ending in birth or egg hatching Inferred from mutant phenotype PubMed 10511553. Source: WormBase embryonic digestive tract development Inferred from mutant phenotype PubMed 10511553. Source: WormBase histone lysine methylation Inferred from electronic annotation. Source: GOC locomotion Inferred from mutant phenotype PubMed 10511553. Source: WormBase nematode larval development Inferred from mutant phenotype PubMed 10511553. Source: WormBase oviposition Inferred from mutant phenotype PubMed 10511553. Source: WormBase positive regulation of transcription from RNA polymerase II promoter Inferred from mutant phenotype PubMed 10511553. Source: WormBase regulation of multicellular organism growth Inferred from mutant phenotype PubMed 10511553. Source: WormBase tail tip morphogenesis Inferred from mutant phenotype PubMed 10511553. Source: WormBase transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	DNA binding Inferred from electronic annotation. Source: InterPro histone-lysine N-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1312	1312	Probable histone-lysine N-methyltransferase lin-59		PRO_0000084431
Regions
	Domain	590 – 635	46	AWS
	Domain	637 – 756	120	SET
	Domain	1100 – 1223	124	BAH
	Zinc finger	967 – 1027	61	PHD-type
	Compositional bias	195 – 230	36	Asp-rich

Sequences

	Sequence		Length	Mass (Da)
	O44757 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9DC16CEF07E65163	FASTA	1,312	147,583
	10 20 30 40 50 60 MHGAGEQQQR YRYNARSDEQ HHHPSTSSHQ YQQQGARQMH QMHPIQATLM CTPTTTSAAA 70 80 90 100 110 120 STSSSGGSNS SGGSGGHRQQ GNILRIGNSI KIGDNILEPA GTLVFSQADG TPWTGQRIVV 130 140 150 160 170 180 NGSTHAVVKA NLFPIGTTPP VLNMQSNQNW YMNQPSGTVP MSSNAPATTS SATPDSGIQS 190 200 210 220 230 240 VPTSPPSPSY AMMNDVDIGD HDDEEDDDGP ADFTDMPLLK PVDEDDDCYD VPCTSEGPPP 250 260 270 280 290 300 NNSNPAITTI PSSCSTPAPP KESLPTGMNV EEWGSFLIAS NMDREEIVRR LMAHDPEMAK 310 320 330 340 350 360 AIAMRIRELS AEESKKKKDM EAEVSSTTPT TPRARGTRTR NKCATRSTNS PDVTTSNLPE 370 380 390 400 410 420 EPSTSTMGLV KENEDVEKVE GKRRGRKPKK RRGFHKESFE DLESDAKKSK AEQHEDHLPE 430 440 450 460 470 480 ASCSSRPESV IPPPVDPIQF RLKVREMMER KLEQLTQKMS EDMTELRLSH LTSSKMVNGE 490 500 510 520 530 540 RGKRRESFLR QLNEQSKKLR KGGMLGRKRL RMFMTESDIL EENKDNIKKE VKEESTPPPT 550 560 570 580 590 600 KLRGRLPSRR TREPSEIVNP EPVEKKFNGE YFEITKSVPS SDDIIPLWMA PSLTCGCTKG 610 620 630 640 650 660 ACTSDMDCLN RALRVQCSSD CSVPYCSNRR FWKEDCGNKL CVSNGPRSKR VLKTKIARRA 670 680 690 700 710 720 GEFLCEYAGE VITREQAQEK FAQDRDPRII AIAAHLFVDA TKRSNIARFI KHSCKPNSRL 730 740 750 760 770 780 EVWSVNGFYR AGVFALSDLN PNAEITVDKS DLLPFDMACN CGATECKRVI RGVRWRCADP 790 800 810 820 830 840 NEKIVTRRFV IRNRRKTIER SSHSGLPAIL QTPMDENSSI RLKMKQVLAA FAFRVRKIDG 850 860 870 880 890 900 SMSRTMLPHY TLIIKFLKTK GNNPNPVEFV SLFRKWLEAI DDDDLERAFV AIESHYMSSS 910 920 930 940 950 960 ILSSSLQSKK AKDNAPRARA LSTSCPSPVP SKRGDADLSY LESLYPIGSY DPDDAWESYS 970 980 990 1000 1010 1020 TNKKGNAVRC ICGALDEEGT MVQCDTCHFW LHVDCCQYVV RSNEKAQKSK NPPSDDGEYI 1030 1040 1050 1060 1070 1080 CDFCTNKQNG LRPSADVKLT EQPDVRFENC DYYRSLINRR GIQVVLNETV YVNRVLPEDH 1090 1100 1110 1120 1130 1140 KAMLRNLREE KKGSKQKDTN KYRFPKAATS PLPIEKVDRK NARIFRVERL FVCPGNNRFV 1150 1160 1170 1180 1190 1200 FGSFYAWPHE TYADAGRVFS KKEVFATPYY ETLPLDEVIG RCLVLDTATW CKGRPKVPKF 1210 1220 1230 1240 1250 1260 KEDDVFLCEM QIGKTQRVFE KVPPKNRYPI NTNSYVFTEF THPKKVVRDF RPYDPSNPSP 1270 1280 1290 1300 1310 KPPKTSSIPS TSSIDPPQSS SDGLPEVDTK KLSKRHIQRV LKRLVKNGSR RS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The bromodomain protein LIN-49 and trithorax-related protein LIN-59 affect development and gene expression in Caenorhabditis elegans." Chamberlin H.M., Thomas J.H. Development 127:713-723(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF163019 mRNA. Translation: AAD49324.1. FO081191 Genomic DNA. Translation: CCD69785.1.
PIR	T32758.
RefSeq	NP_491206.1. NM_058805.5.
UniGene	Cel.17896.
3D structure databases
ProteinModelPortal	O44757.
SMR	O44757. Positions 583-772, 966-1026, 1127-1248.
ModBase	Search...
Protein-protein interaction databases
MINT	MINT-3385943.
STRING	6239.T12F5.4.
Proteomic databases
PaxDb	O44757.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	T12F5.4; T12F5.4; T12F5.4.
GeneID	266825.
KEGG	cel:CELE_T12F5.4.
UCSC	T12F5.4. c. elegans.
Organism-specific databases
CTD	266825.
WormBase	T12F5.4; CE13601; WBGene00003040; lin-59.
Phylogenomic databases
eggNOG	COG2940.
GeneTree	ENSGT00700000104009.
InParanoid	O44757.
KO	K06101.
OMA	MEAEVSS.
Family and domain databases
Gene3D	3.30.40.10. 1 hit.
InterPro	IPR006560. AWS. IPR001025. BAH_dom. IPR001214. SET_dom. IPR019786. Zinc_finger_PHD-type_CS. IPR011011. Znf_FYVE_PHD. IPR001965. Znf_PHD. IPR019787. Znf_PHD-finger. IPR013083. Znf_RING/FYVE/PHD. [Graphical view]
Pfam	PF01426. BAH. 1 hit. PF00628. PHD. 1 hit. PF00856. SET. 1 hit. [Graphical view]
SMART	SM00570. AWS. 1 hit. SM00439. BAH. 1 hit. SM00249. PHD. 1 hit. SM00317. SET. 1 hit. [Graphical view]
SUPFAM	SSF57903. FYVE_PHD_ZnF. 1 hit.
PROSITE	PS51215. AWS. 1 hit. PS51038. BAH. 1 hit. PS50280. SET. 1 hit. PS01359. ZF_PHD_1. 1 hit. PS50016. ZF_PHD_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	953172.

Entry information

Entry name

LIN59_CAEEL

Accession

Primary (citable) accession number: O44757

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 98 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project