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Protein

M-phase inducer phosphatase cdc-25.2

Gene

cdc-25.2

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Required for intestinal cell division following the 16E cell stage of embryogenesis. Regulates intestinal cell divisions and binucleations probably by modulating the activity of the cell cycle regulator wee-1.3 and by activating the cdk-1/cyb-1 complex.1 Publication

Catalytic activityi

Protein tyrosine phosphate + H2O = protein tyrosine + phosphate.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protein phosphatase
Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

ReactomeiR-CEL-156711. Polo-like kinase mediated events.
R-CEL-176187. Activation of ATR in response to replication stress.
R-CEL-5625740. RHO GTPases activate PKNs.
R-CEL-69273. Cyclin A/B1 associated events during G2/M transition.
R-CEL-69601. Ubiquitin Mediated Degradation of Phosphorylated Cdc25A.
R-CEL-69610. p53-Independent DNA Damage Response.
R-CEL-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
M-phase inducer phosphatase cdc-25.2 (EC:3.1.3.48)
Alternative name(s):
Cell division cycle-related protein 25.2
Gene namesi
Name:cdc-25.2Imported
ORF Names:F16B4.8Imported
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome V

Organism-specific databases

WormBaseiF16B4.8a; CE19796; WBGene00000387; cdc-25.2.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Disruption phenotypei

Viable, but 85% of animals are sterile at 20 degrees Celsius. Reduced number of intestinal cells in 1.5-fold stage embryos due to defective intestinal divisions and binuleations at the 16E cell stage of embryonic development. Knockout with wee-1.3 RNAi suppresses the defect in intestinal cell divisions in the cdc-25.2 single mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001986561 – 480M-phase inducer phosphatase cdc-25.2Add BLAST480

Proteomic databases

PaxDbiO44628.

Expressioni

Developmental stagei

Transiently expressed in the intestine during larval development. Expressed in the intestine in L1 stage larvae, not expressed in L2 to L3 stage larvae, but is then again expressed in the gonad of L4 stage larvae.1 Publication

Gene expression databases

BgeeiWBGene00000387.
ExpressionAtlasiO44628. baseline.

Interactioni

Protein-protein interaction databases

STRINGi6239.F16B4.8.

Structurei

3D structure databases

ProteinModelPortaliO44628.
SMRiO44628.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini243 – 349RhodanesePROSITE-ProRule annotationAdd BLAST107

Sequence similaritiesi

Belongs to the MPI phosphatase family.Curated

Phylogenomic databases

eggNOGiKOG3772. Eukaryota.
COG5105. LUCA.
GeneTreeiENSGT00390000018747.
InParanoidiO44628.
OMAiSGICELM.
OrthoDBiEOG091G0866.
PhylomeDBiO44628.

Family and domain databases

Gene3Di3.40.250.10. 1 hit.
InterProiView protein in InterPro
IPR000751. MPI_Phosphatase.
IPR001763. Rhodanese-like_dom.
PfamiView protein in Pfam
PF00581. Rhodanese. 1 hit.
PRINTSiPR00716. MPIPHPHTASE.
SMARTiView protein in SMART
SM00450. RHOD. 1 hit.
SUPFAMiSSF52821. SSF52821. 1 hit.
PROSITEiView protein in PROSITE
PS50206. RHODANESE_3. 1 hit.

Sequencei

Sequence statusi: Complete.

O44628-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRPSQISQD VAQPLSNQHE TAMMSSDEDS MSRDSGICEL MDENTPVCFS
60 70 80 90 100
SMSTESTTVQ VEECMEIDED ENLQPGPVSR RQKKVTFRRE KSSCQVRLFD
110 120 130 140 150
ESPTSHKMFM RPQAPLSVRS ENQQLLQQRK RKFEKSEQSS DHFHLEPMSV
160 170 180 190 200
EAMDDDEIVM MDQNTTKWEP GFRAPNHKKG RLTRSATAFP SLETFEEEEH
210 220 230 240 250
EEQHHLNVKY HLKTVAKESS KGFRRITAET LRDIFFRLSE KEFDDKYILI
260 270 280 290 300
DCRYPYEYNR GHIKNAINHF DRVTVSKIFY DENGRKRCNK IPIFYCEFSQ
310 320 330 340 350
ARGPKMAYAL RQVDRELNVN HYPKCDYEEM YVLDLGYRNF FFAANEANIT
360 370 380 390 400
NLCQPHAYCE MHDKEHTMEL KKYNFHNKGQ SVLRTVSMSR SFKSLPTGSA
410 420 430 440 450
FNFVASSASF TSAPSTSTEN IDTNDDCQKS RTPAVPRIAS RRNLFSDPSH
460 470 480
SPTNFAQFPL TCSRETPSPH KHPLTCPRFS
Length:480
Mass (Da):55,737
Last modified:October 1, 2001 - v2
Checksum:i19B11B138D582FA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081161 Genomic DNA. Translation: CCD69573.1.
PIRiB88953.
T32663.
T32665.
RefSeqiNP_503446.1. NM_071045.3.
UniGeneiCel.23180.

Genome annotation databases

EnsemblMetazoaiF16B4.8a; F16B4.8a; WBGene00000387.
GeneIDi178645.
KEGGicel:CELE_F16B4.8.
UCSCiF16B4.8. c. elegans.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiMPIP2_CAEEL
AccessioniPrimary (citable) accession number: O44628
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: October 1, 2001
Last modified: July 5, 2017
This is version 118 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families