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Protein

Mitogen-activated protein kinase pmk-3

Gene

pmk-3

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Activated by phosphorylation on threonine and tyrosine.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei150 – 1501ATPPROSITE-ProRule annotation
Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
  3. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. MAPK cascade Source: WormBase
  2. MAPK cascade involved in axon regeneration Source: WormBase
  3. regulation of synapse organization Source: WormBase
  4. response to osmotic stress Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_283285. p38MAPK events.
REACT_283732. VEGFA-VEGFR2 Pathway.
REACT_284305. activated TAK1 mediates p38 MAPK activation.
REACT_299710. NOD1/2 Signaling Pathway.
REACT_315402. Platelet sensitization by LDL.
REACT_331670. ERK/MAPK targets.
REACT_335479. CDO in myogenesis.
REACT_336620. Oxidative Stress Induced Senescence.
REACT_350756. KSRP destabilizes mRNA.
REACT_354922. ADP signalling through P2Y purinoceptor 1.
SignaLinkiO44514.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitogen-activated protein kinase pmk-3 (EC:2.7.11.24)
Alternative name(s):
Stress-activated protein kinase pmk-3
p38 MAP kinase 3
Gene namesi
Name:pmk-3
ORF Names:F42G8.4
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiF42G8.4; CE29318; WBGene00004057; pmk-3.

Subcellular locationi

Nucleus 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: WormBase
  2. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 474474Mitogen-activated protein kinase pmk-3PRO_0000186305Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei285 – 2851PhosphothreonineBy similarity
Modified residuei287 – 2871PhosphotyrosineBy similarity

Post-translational modificationi

Dually phosphorylated on Thr-285 and Tyr-287, which activates the enzyme.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO44514.

Expressioni

Tissue specificityi

Expressed throughout the intestine.1 Publication

Gene expression databases

ExpressionAtlasiO44514. baseline.

Interactioni

Protein-protein interaction databases

BioGridi42723. 1 interaction.
DIPiDIP-59691N.
STRINGi6239.F42G8.4.2.

Structurei

3D structure databases

ProteinModelPortaliO44514.
SMRiO44514. Positions 67-459.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini114 – 419306Protein kinasePROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi285 – 2873TXY

Domaini

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
InParanoidiO44514.
KOiK08293.
OMAiRADHIFD.
OrthoDBiEOG7W6WQH.
PhylomeDBiO44514.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O44514-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASVPSSSSL PVSHVRRHED VSTPSAPPTK RSNNQSQPPE SYEPNTWLQQ
60 70 80 90 100
QREQEQQKKL AAENIKKQSI EATGNNEMVG EEEEDILSKP CGPHKRRFQF
110 120 130 140 150
VMIRNITFAI PEGYDVEPNS IEYLGGGSFG NVIKTSAVCR DGLRRYVAIK
160 170 180 190 200
KMREPFFDPH HARRIFRETK LLQLMRHDNI ICALDIYTPD EENDFRDVYV
210 220 230 240 250
VTEFAGRSLY QILKQQRDYG RRVLTDEHIK FIIYQIIRAL KYIHSANIIH
260 270 280 290 300
RDLKPGNLAL TDDSDLMILD FGLARSLEKK DTSLTQYVQT RWYRSPEVIY
310 320 330 340 350
WKIDSYTNLA DMWSLGCIAA ELLTGEPLFP GDEPNAQYQR ITQLCGSPDE
360 370 380 390 400
ELLTKIENDN SSAIKAVIQS YTTHKRRNFR DVFSAHNPSE DFIDLLEKLL
410 420 430 440 450
VLDPEKRITV EEAIQHPYLA EFSLPEDEPR ADHIFDLDDS QARTRFEWRD
460 470
AVWKEIMNYK RLSSSPLIPG EADR
Length:474
Mass (Da):54,894
Last modified:December 1, 2001 - v2
Checksum:iE405C45FDCC6ABA9
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080126 Genomic DNA. Translation: CCD61404.1.
PIRiT32642.
RefSeqiNP_501363.1. NM_068962.4.
UniGeneiCel.12533.

Genome annotation databases

EnsemblMetazoaiF42G8.4a.1; F42G8.4a.1; WBGene00004057.
F42G8.4a.2; F42G8.4a.2; WBGene00004057.
GeneIDi177610.
KEGGicel:CELE_F42G8.4.
UCSCiF42G8.4.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080126 Genomic DNA. Translation: CCD61404.1.
PIRiT32642.
RefSeqiNP_501363.1. NM_068962.4.
UniGeneiCel.12533.

3D structure databases

ProteinModelPortaliO44514.
SMRiO44514. Positions 67-459.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42723. 1 interaction.
DIPiDIP-59691N.
STRINGi6239.F42G8.4.2.

Proteomic databases

PaxDbiO44514.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiF42G8.4a.1; F42G8.4a.1; WBGene00004057.
F42G8.4a.2; F42G8.4a.2; WBGene00004057.
GeneIDi177610.
KEGGicel:CELE_F42G8.4.
UCSCiF42G8.4.1. c. elegans.

Organism-specific databases

CTDi177610.
WormBaseiF42G8.4; CE29318; WBGene00004057; pmk-3.

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00550000074271.
HOGENOMiHOG000233024.
InParanoidiO44514.
KOiK08293.
OMAiRADHIFD.
OrthoDBiEOG7W6WQH.
PhylomeDBiO44514.

Enzyme and pathway databases

ReactomeiREACT_283285. p38MAPK events.
REACT_283732. VEGFA-VEGFR2 Pathway.
REACT_284305. activated TAK1 mediates p38 MAPK activation.
REACT_299710. NOD1/2 Signaling Pathway.
REACT_315402. Platelet sensitization by LDL.
REACT_331670. ERK/MAPK targets.
REACT_335479. CDO in myogenesis.
REACT_336620. Oxidative Stress Induced Senescence.
REACT_350756. KSRP destabilizes mRNA.
REACT_354922. ADP signalling through P2Y purinoceptor 1.
SignaLinkiO44514.

Miscellaneous databases

NextBioi897580.

Gene expression databases

ExpressionAtlasiO44514. baseline.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
    Berman K., McKay J., Avery L., Cobb M.
    Mol. Cell Biol. Res. Commun. 4:337-344(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.

Entry informationi

Entry nameiPMK3_CAEEL
AccessioniPrimary (citable) accession number: O44514
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: April 1, 2015
This is version 122 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.