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O44514 (PMK3_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 115. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitogen-activated protein kinase pmk-3

EC=2.7.11.24
Alternative name(s):
Stress-activated protein kinase pmk-3
p38 MAP kinase 3
Gene names
Name:pmk-3
ORF Names:F42G8.4
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets. Ref.1

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. Ref.1

Cofactor

Magnesium. Ref.1

Enzyme regulation

Activated by phosphorylation on threonine and tyrosine By similarity. UniProtKB Q16539

Subcellular location

Nucleus Ref.1.

Tissue specificity

Expressed throughout the intestine. Ref.1

Domain

The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

Post-translational modification

Dually phosphorylated on Thr-285 and Tyr-287, which activates the enzyme By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Mitogen-activated protein kinase pmk-3
PRO_0000186305

Regions

Domain114 – 419306Protein kinase
Nucleotide binding124 – 1329ATP By similarity UniProtKB Q16539
Motif285 – 2873TXY

Sites

Active site2521Proton acceptor By similarity UniProtKB Q16539
Binding site1501ATP By similarity UniProtKB Q16539

Amino acid modifications

Modified residue2851Phosphothreonine By similarity UniProtKB Q16539
Modified residue2871Phosphotyrosine By similarity UniProtKB Q16539

Sequences

Sequence LengthMass (Da)Tools
O44514 [UniParc].

Last modified December 1, 2001. Version 2.
Checksum: E405C45FDCC6ABA9

FASTA47454,894
        10         20         30         40         50         60 
MASVPSSSSL PVSHVRRHED VSTPSAPPTK RSNNQSQPPE SYEPNTWLQQ QREQEQQKKL 

        70         80         90        100        110        120 
AAENIKKQSI EATGNNEMVG EEEEDILSKP CGPHKRRFQF VMIRNITFAI PEGYDVEPNS 

       130        140        150        160        170        180 
IEYLGGGSFG NVIKTSAVCR DGLRRYVAIK KMREPFFDPH HARRIFRETK LLQLMRHDNI 

       190        200        210        220        230        240 
ICALDIYTPD EENDFRDVYV VTEFAGRSLY QILKQQRDYG RRVLTDEHIK FIIYQIIRAL 

       250        260        270        280        290        300 
KYIHSANIIH RDLKPGNLAL TDDSDLMILD FGLARSLEKK DTSLTQYVQT RWYRSPEVIY 

       310        320        330        340        350        360 
WKIDSYTNLA DMWSLGCIAA ELLTGEPLFP GDEPNAQYQR ITQLCGSPDE ELLTKIENDN 

       370        380        390        400        410        420 
SSAIKAVIQS YTTHKRRNFR DVFSAHNPSE DFIDLLEKLL VLDPEKRITV EEAIQHPYLA 

       430        440        450        460        470 
EFSLPEDEPR ADHIFDLDDS QARTRFEWRD AVWKEIMNYK RLSSSPLIPG EADR 

« Hide

References

« Hide 'large scale' references
[1]"Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
Berman K., McKay J., Avery L., Cobb M.
Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
FO080126 Genomic DNA. Translation: CCD61404.1.
PIRT32642.
RefSeqNP_501363.1. NM_068962.4.
UniGeneCel.12533.

3D structure databases

ProteinModelPortalO44514.
SMRO44514. Positions 67-459.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid42723. 1 interaction.
DIPDIP-59691N.
STRING6239.F42G8.4.2.

Proteomic databases

PaxDbO44514.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaF42G8.4.1; F42G8.4.1; WBGene00004057.
F42G8.4.2; F42G8.4.2; WBGene00004057.
GeneID177610.
KEGGcel:CELE_F42G8.4.
UCSCF42G8.4.1. c. elegans.

Organism-specific databases

CTD177610.
WormBaseF42G8.4; CE29318; WBGene00004057; pmk-3.

Phylogenomic databases

eggNOGCOG0515.
GeneTreeENSGT00550000074271.
HOGENOMHOG000233024.
InParanoidO44514.
KOK08293.
OMAEENDFRD.
OrthoDBEOG7W6WQH.
PhylomeDBO44514.

Enzyme and pathway databases

SignaLinkO44514.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR003527. MAP_kinase_CS.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
[Graphical view]
PfamPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS01351. MAPK. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio897580.

Entry information

Entry namePMK3_CAEEL
AccessionPrimary (citable) accession number: O44514
Entry history
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: December 1, 2001
Last modified: June 11, 2014
This is version 115 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase