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O44514

- PMK3_CAEEL

UniProt

O44514 - PMK3_CAEEL

Protein

Mitogen-activated protein kinase pmk-3

Gene

pmk-3

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 Dec 2001)
      Previous versions | rss
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    Functioni

    Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating downstream targets.1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.1 Publication

    Cofactori

    Magnesium.1 Publication

    Enzyme regulationi

    Activated by phosphorylation on threonine and tyrosine.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei150 – 1501ATPPROSITE-ProRule annotation
    Active sitei252 – 2521Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi124 – 1329ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. protein serine/threonine kinase activity Source: UniProtKB

    GO - Biological processi

    1. MAPK cascade Source: WormBase
    2. regulation of synapse organization Source: WormBase
    3. response to osmotic stress Source: UniProtKB

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    SignaLinkiO44514.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitogen-activated protein kinase pmk-3 (EC:2.7.11.24)
    Alternative name(s):
    Stress-activated protein kinase pmk-3
    p38 MAP kinase 3
    Gene namesi
    Name:pmk-3
    ORF Names:F42G8.4
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiF42G8.4; CE29318; WBGene00004057; pmk-3.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: WormBase
    2. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 474474Mitogen-activated protein kinase pmk-3PRO_0000186305Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei285 – 2851PhosphothreonineBy similarity
    Modified residuei287 – 2871PhosphotyrosineBy similarity

    Post-translational modificationi

    Dually phosphorylated on Thr-285 and Tyr-287, which activates the enzyme.By similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiO44514.

    Expressioni

    Tissue specificityi

    Expressed throughout the intestine.1 Publication

    Interactioni

    Protein-protein interaction databases

    BioGridi42723. 1 interaction.
    DIPiDIP-59691N.
    STRINGi6239.F42G8.4.2.

    Structurei

    3D structure databases

    ProteinModelPortaliO44514.
    SMRiO44514. Positions 67-459.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini114 – 419306Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi285 – 2873TXY

    Domaini

    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00550000074271.
    HOGENOMiHOG000233024.
    InParanoidiO44514.
    KOiK08293.
    OMAiEENDFRD.
    OrthoDBiEOG7W6WQH.
    PhylomeDBiO44514.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O44514-1 [UniParc]FASTAAdd to Basket

    « Hide

    MASVPSSSSL PVSHVRRHED VSTPSAPPTK RSNNQSQPPE SYEPNTWLQQ    50
    QREQEQQKKL AAENIKKQSI EATGNNEMVG EEEEDILSKP CGPHKRRFQF 100
    VMIRNITFAI PEGYDVEPNS IEYLGGGSFG NVIKTSAVCR DGLRRYVAIK 150
    KMREPFFDPH HARRIFRETK LLQLMRHDNI ICALDIYTPD EENDFRDVYV 200
    VTEFAGRSLY QILKQQRDYG RRVLTDEHIK FIIYQIIRAL KYIHSANIIH 250
    RDLKPGNLAL TDDSDLMILD FGLARSLEKK DTSLTQYVQT RWYRSPEVIY 300
    WKIDSYTNLA DMWSLGCIAA ELLTGEPLFP GDEPNAQYQR ITQLCGSPDE 350
    ELLTKIENDN SSAIKAVIQS YTTHKRRNFR DVFSAHNPSE DFIDLLEKLL 400
    VLDPEKRITV EEAIQHPYLA EFSLPEDEPR ADHIFDLDDS QARTRFEWRD 450
    AVWKEIMNYK RLSSSPLIPG EADR 474
    Length:474
    Mass (Da):54,894
    Last modified:December 1, 2001 - v2
    Checksum:iE405C45FDCC6ABA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080126 Genomic DNA. Translation: CCD61404.1.
    PIRiT32642.
    RefSeqiNP_501363.1. NM_068962.4.
    UniGeneiCel.12533.

    Genome annotation databases

    EnsemblMetazoaiF42G8.4.1; F42G8.4.1; WBGene00004057.
    F42G8.4.2; F42G8.4.2; WBGene00004057.
    GeneIDi177610.
    KEGGicel:CELE_F42G8.4.
    UCSCiF42G8.4.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    FO080126 Genomic DNA. Translation: CCD61404.1 .
    PIRi T32642.
    RefSeqi NP_501363.1. NM_068962.4.
    UniGenei Cel.12533.

    3D structure databases

    ProteinModelPortali O44514.
    SMRi O44514. Positions 67-459.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 42723. 1 interaction.
    DIPi DIP-59691N.
    STRINGi 6239.F42G8.4.2.

    Proteomic databases

    PaxDbi O44514.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai F42G8.4.1 ; F42G8.4.1 ; WBGene00004057 .
    F42G8.4.2 ; F42G8.4.2 ; WBGene00004057 .
    GeneIDi 177610.
    KEGGi cel:CELE_F42G8.4.
    UCSCi F42G8.4.1. c. elegans.

    Organism-specific databases

    CTDi 177610.
    WormBasei F42G8.4 ; CE29318 ; WBGene00004057 ; pmk-3.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00550000074271.
    HOGENOMi HOG000233024.
    InParanoidi O44514.
    KOi K08293.
    OMAi EENDFRD.
    OrthoDBi EOG7W6WQH.
    PhylomeDBi O44514.

    Enzyme and pathway databases

    SignaLinki O44514.

    Miscellaneous databases

    NextBioi 897580.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR003527. MAP_kinase_CS.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS01351. MAPK. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation and characterization of pmk-(1-3): three p38 homologs in Caenorhabditis elegans."
      Berman K., McKay J., Avery L., Cobb M.
      Mol. Cell Biol. Res. Commun. 4:337-344(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.

    Entry informationi

    Entry nameiPMK3_CAEEL
    AccessioniPrimary (citable) accession number: O44514
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: December 1, 2001
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3