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Protein

Pyruvate dehydrogenase E1 component subunit beta, mitochondrial

Gene

pdhb-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO2. It contains multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3) (By similarity).By similarity

Catalytic activityi

Pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S-acetyldihydrolipoyllysine + CO2.By similarity

Cofactori

thiamine diphosphateBy similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811Thiamine pyrophosphateBy similarity

GO - Molecular functioni

  1. pyruvate dehydrogenase (acetyl-transferring) activity Source: UniProtKB-EC

GO - Biological processi

  1. acetyl-CoA biosynthetic process from pyruvate Source: InterPro
  2. glycolytic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

Pyruvate, Thiamine pyrophosphate

Enzyme and pathway databases

ReactomeiREACT_257024. Pyruvate metabolism.
REACT_263254. Regulation of pyruvate dehydrogenase (PDH) complex.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate dehydrogenase E1 component subunit beta, mitochondrial (EC:1.2.4.1)
Short name:
PDHE1-B
Gene namesi
Name:pdhb-1
ORF Names:C04C3.3
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiC04C3.3; CE27647; WBGene00015413; pdhb-1.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
  2. mitochondrion Source: WormBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2121MitochondrionSequence AnalysisAdd
BLAST
Chaini22 – 352331Pyruvate dehydrogenase E1 component subunit beta, mitochondrialPRO_0000042638Add
BLAST

Proteomic databases

PaxDbiO44451.
PRIDEiO44451.

Interactioni

Subunit structurei

Tetramer of 2 alpha and 2 beta subunits.By similarity

Protein-protein interaction databases

BioGridi42248. 6 interactions.
DIPiDIP-24348N.
IntActiO44451. 5 interactions.
MINTiMINT-1052850.
STRINGi6239.C04C3.3.2.

Structurei

3D structure databases

ProteinModelPortaliO44451.
SMRiO44451. Positions 25-351.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0022.
GeneTreeiENSGT00530000063423.
HOGENOMiHOG000281450.
InParanoidiO44451.
KOiK00162.
OMAiDIPTPYN.
PhylomeDBiO44451.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view]
PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O44451-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MALRKCGNLF VARLAGTSTR AASTMTVRDA LNQAMDEEIK RDDRVFLMGE
60 70 80 90 100
EVAQYDGAYK ISKGLWKKHG DKRVVDTPIT EMGFAGIAVG AAFAGLRPIC
110 120 130 140 150
EFMTFNFSMQ AIDQIINSAA KTYYMSAGRV PVPIVFRGPN GAAAGVAAQH
160 170 180 190 200
SQDFSAWYAH CPGLKVVCPY SAEDAKGLLK AAIRDDNPVV FLENEILYGQ
210 220 230 240 250
SFPVGDEVLS DDFVVPIGKA KIERAGDHVT IVSYSRGVEF SLEAAKQLEA
260 270 280 290 300
IGVSAEVINL RSLRPFDFES IRQSVHKTHH LVSVETGWPF AGIGSEIAAQ
310 320 330 340 350
VMESDVFDQL DAPLLRVTGV DVPMPYTQTL EAAALPTAEH VVKAVKKSLN

IA
Length:352
Mass (Da):38,141
Last modified:October 1, 2001 - v2
Checksum:i37F4A4A28C8ADECA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080230 Genomic DNA. Translation: CCD62196.1.
PIRiT32598.
RefSeqiNP_500340.1. NM_067939.5.
UniGeneiCel.7275.

Genome annotation databases

EnsemblMetazoaiC04C3.3; C04C3.3; WBGene00015413.
GeneIDi177108.
KEGGicel:CELE_C04C3.3.
UCSCiC04C3.3.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO080230 Genomic DNA. Translation: CCD62196.1.
PIRiT32598.
RefSeqiNP_500340.1. NM_067939.5.
UniGeneiCel.7275.

3D structure databases

ProteinModelPortaliO44451.
SMRiO44451. Positions 25-351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42248. 6 interactions.
DIPiDIP-24348N.
IntActiO44451. 5 interactions.
MINTiMINT-1052850.
STRINGi6239.C04C3.3.2.

Proteomic databases

PaxDbiO44451.
PRIDEiO44451.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC04C3.3; C04C3.3; WBGene00015413.
GeneIDi177108.
KEGGicel:CELE_C04C3.3.
UCSCiC04C3.3.1. c. elegans.

Organism-specific databases

CTDi177108.
WormBaseiC04C3.3; CE27647; WBGene00015413; pdhb-1.

Phylogenomic databases

eggNOGiCOG0022.
GeneTreeiENSGT00530000063423.
HOGENOMiHOG000281450.
InParanoidiO44451.
KOiK00162.
OMAiDIPTPYN.
PhylomeDBiO44451.

Enzyme and pathway databases

ReactomeiREACT_257024. Pyruvate metabolism.
REACT_263254. Regulation of pyruvate dehydrogenase (PDH) complex.

Miscellaneous databases

NextBioi895372.
PROiO44451.

Family and domain databases

Gene3Di3.40.50.920. 1 hit.
3.40.50.970. 1 hit.
InterProiIPR027110. PDHB.
IPR029061. THDP-binding.
IPR009014. Transketo_C/Pyr-ferredox_oxred.
IPR005475. Transketolase-like_Pyr-bd.
IPR005476. Transketolase_C.
[Graphical view]
PANTHERiPTHR11624:SF56. PTHR11624:SF56. 1 hit.
PfamiPF02779. Transket_pyr. 1 hit.
PF02780. Transketolase_C. 1 hit.
[Graphical view]
SMARTiSM00861. Transket_pyr. 1 hit.
[Graphical view]
SUPFAMiSSF52518. SSF52518. 1 hit.
SSF52922. SSF52922. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  2. Bienvenut W.V.
    Submitted (SEP-2005) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 130-137; 237-246 AND 262-272, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiODPB_CAEEL
AccessioniPrimary (citable) accession number: O44451
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 25, 2005
Last sequence update: October 1, 2001
Last modified: January 7, 2015
This is version 106 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.