O44437 (SMD3_DROME) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Small nuclear ribonucleoprotein Sm D3
Short name=Sm-D3

Alternative name(s):
snRNP core protein D3

Gene names

Name:	SmD3
Synonyms:	guf2
ORF Names:	CG8427

Organism

Drosophila melanogaster (Fruit fly) [Reference proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Sophophora › melanogaster group › melanogaster subgroup

Protein attributes

Sequence length	151 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for pre-mRNA splicing. Required for snRNP biogenesis By similarity.
Subcellular location	Nucleus By similarity.
Post-translational modification	Methylated on arginine residues by Art5 and Art7; methylation is not required for assembly and biogenesis of snRNPs. Ref.5
Sequence similarities	Belongs to the snRNP core protein family.

Ontologies

Keywords
Biological process	mRNA processing mRNA splicing
Cellular component	Nucleus
Domain	Repeat
Molecular function	Ribonucleoprotein
PTM	Methylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	central nervous system development Inferred from mutant phenotype PubMed 8224829PubMed 8878684. Source: FlyBase lymph gland development Inferred from mutant phenotype PubMed 8224829. Source: FlyBase mRNA splicing, via spliceosome Inferred from sequence or structural similarity PubMed 10908584. Source: FlyBase mitosis Inferred from mutant phenotype PubMed 18463163. Source: FlyBase mitotic spindle organization Inferred from mutant phenotype PubMed 17412918. Source: FlyBase muscle organ development Inferred from mutant phenotype PubMed 8878684. Source: FlyBase neuron differentiation Inferred from mutant phenotype PubMed 8878684. Source: FlyBase peripheral nervous system development Inferred from mutant phenotype PubMed 7789767PubMed 8878684. Source: FlyBase pole plasm oskar mRNA localization Inferred from mutant phenotype PubMed 20570937. Source: FlyBase
Cellular_component	catalytic step 2 spliceosome Inferred from direct assay PubMed 18981222. Source: FlyBase pole plasm Inferred from direct assay PubMed 20570937PubMed 20659974. Source: FlyBase precatalytic spliceosome Inferred from direct assay PubMed 18981222. Source: FlyBase small nuclear ribonucleoprotein complex Inferred from sequence or structural similarity PubMed 10908584. Source: FlyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 151	151	Small nuclear ribonucleoprotein Sm D3		PRO_0000122218
Regions
	Repeat	108 – 109	2	1
	Repeat	110 – 111	2	2
	Repeat	112 – 113	2	3
	Region	108 – 113	6	3 X 2 AA tandem repeats of R-G
	Compositional bias	84 – 120	37	Arg/Lys-rich (basic)

Sequences

	Sequence		Length	Mass (Da)
	O44437 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 65F0B899EACA1C77	FASTA	151	15,582
	10 20 30 40 50 60 MSIGVPIKVL HEAEGHIITC ETITGEVYRG KLIEAEDNMN CQMTQITVTY RDGRTANLEN 70 80 90 100 110 120 VYIRGSKIRF LILPDMLKNA PMFKKQTGKG LGGTAGRGKA AILRAQARGR GRGGPPGGGR 130 140 150 GTGGPPGAPG GSGGRGAWQG GPTGGRGRGG L

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand." Ivanov I.P., Simin K., Letsou A., Atkins J.F., Gesteland R.F. Mol. Cell. Biol. 18:1553-1561(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Embryo.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed] [Europe PMC] [Abstract] Cited for: GENOME REANNOTATION. Strain: Berkeley.
[4]	"A Drosophila full-length cDNA resource." Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E. Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: Berkeley. Tissue: Embryo.
[5]	"Sm protein methylation is dispensable for snRNP assembly in Drosophila melanogaster." Gonsalvez G.B., Praveen K., Hicks A.J., Tian L., Matera A.G. RNA 14:878-887(2008) [PubMed] [Europe PMC] [Abstract] Cited for: METHYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF038598 mRNA. Translation: AAB92621.1. AE013599 Genomic DNA. Translation: AAF58566.1. AY113465 mRNA. Translation: AAM29470.1.
RefSeq	NP_725106.1. NM_165866.2.
UniGene	Dm.19912.
3D structure databases
ProteinModelPortal	O44437.
SMR	O44437. Positions 2-75.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-19470N.
IntAct	O44437. 1 interaction.
MINT	MINT-306370.
STRING	7227.FBpp0087094.
Proteomic databases
PaxDb	O44437.
PRIDE	O44437.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	FBtr0087986; FBpp0087094; FBgn0023167.
GeneID	36306.
KEGG	dme:Dmel_CG8427.
Organism-specific databases
CTD	36306.
FlyBase	FBgn0023167. SmD3.
Phylogenomic databases
eggNOG	COG1958.
GeneTree	ENSGT00610000086153.
InParanoid	O44437.
KO	K11088.
OMA	RNAPMFR.
OrthoDB	EOG44TMRK.
PhylomeDB	O44437.
Gene expression databases
Bgee	O44437.
GermOnline	CG8427. Drosophila melanogaster.
Family and domain databases
InterPro	IPR027141. LSm4/Sm_D1/D3. IPR010920. LSM_dom. IPR001163. Ribonucl_LSM. IPR006649. Ribonucl_LSM_euk/arc. [Graphical view]
PANTHER	PTHR23338. PTHR23338. 1 hit.
Pfam	PF01423. LSM. 1 hit. [Graphical view]
SMART	SM00651. Sm. 1 hit. [Graphical view]
SUPFAM	SSF50182. Sm_like_riboprot. 1 hit.
ProtoNet	Search...
Other
GenomeRNAi	36306.
NextBio	797837.

Entry information

Entry name

SMD3_DROME

Accession

Primary (citable) accession number: O44437

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project