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Protein

Small nuclear ribonucleoprotein Sm D3

Gene

SmD3

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for pre-mRNA splicing. Required for snRNP biogenesis (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • central nervous system development Source: FlyBase
  • lymph gland development Source: FlyBase
  • mitotic nuclear division Source: FlyBase
  • mitotic spindle organization Source: FlyBase
  • mRNA splicing, via spliceosome Source: FlyBase
  • muscle organ development Source: FlyBase
  • neuron differentiation Source: FlyBase
  • peripheral nervous system development Source: FlyBase
  • pole plasm oskar mRNA localization Source: FlyBase
  • regulation of glucose metabolic process Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72165. mRNA Splicing - Minor Pathway.

Names & Taxonomyi

Protein namesi
Recommended name:
Small nuclear ribonucleoprotein Sm D3
Short name:
Sm-D3
Alternative name(s):
snRNP core protein D3
Gene namesi
Name:SmD3
Synonyms:guf2
ORF Names:CG8427
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0023167. SmD3.

Subcellular locationi

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: FlyBase
  • nucleus Source: FlyBase
  • pole plasm Source: FlyBase
  • precatalytic spliceosome Source: FlyBase
  • small nuclear ribonucleoprotein complex Source: FlyBase
  • spliceosomal complex Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 151151Small nuclear ribonucleoprotein Sm D3PRO_0000122218Add
BLAST

Post-translational modificationi

Methylated on arginine residues by Art5 and Art7; methylation is not required for assembly and biogenesis of snRNPs.1 Publication

Keywords - PTMi

Methylation

Proteomic databases

PaxDbiO44437.
PRIDEiO44437.

Expressioni

Gene expression databases

BgeeiO44437.
GenevisibleiO44437. DM.

Interactioni

Subunit structurei

Interacts with the SMN complex.1 Publication

Protein-protein interaction databases

BioGridi62087. 5 interactions.
DIPiDIP-19470N.
IntActiO44437. 26 interactions.
MINTiMINT-306370.
STRINGi7227.FBpp0087094.

Structurei

3D structure databases

ProteinModelPortaliO44437.
SMRiO44437. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati108 – 10921
Repeati110 – 11122
Repeati112 – 11323

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni108 – 11363 X 2 AA tandem repeats of R-G

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi84 – 12037Arg/Lys-rich (basic)Add
BLAST

Sequence similaritiesi

Belongs to the snRNP core protein family.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG3172. Eukaryota.
COG1958. LUCA.
GeneTreeiENSGT00610000086153.
InParanoidiO44437.
KOiK11088.
OMAiEGHVVTC.
OrthoDBiEOG7VTDQF.
PhylomeDBiO44437.

Family and domain databases

InterProiIPR027141. LSm4/Sm_D1/D3.
IPR010920. LSM_dom.
IPR001163. LSM_dom_euk/arc.
[Graphical view]
PANTHERiPTHR23338. PTHR23338. 1 hit.
PfamiPF01423. LSM. 1 hit.
[Graphical view]
SMARTiSM00651. Sm. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.

Sequencei

Sequence statusi: Complete.

O44437-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIGVPIKVL HEAEGHIITC ETITGEVYRG KLIEAEDNMN CQMTQITVTY
60 70 80 90 100
RDGRTANLEN VYIRGSKIRF LILPDMLKNA PMFKKQTGKG LGGTAGRGKA
110 120 130 140 150
AILRAQARGR GRGGPPGGGR GTGGPPGAPG GSGGRGAWQG GPTGGRGRGG

L
Length:151
Mass (Da):15,582
Last modified:June 1, 1998 - v1
Checksum:i65F0B899EACA1C77
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038598 mRNA. Translation: AAB92621.1.
AE013599 Genomic DNA. Translation: AAF58566.1.
AY113465 mRNA. Translation: AAM29470.1.
RefSeqiNP_725106.1. NM_165866.3.
UniGeneiDm.19912.

Genome annotation databases

EnsemblMetazoaiFBtr0087986; FBpp0087094; FBgn0023167.
GeneIDi36306.
KEGGidme:Dmel_CG8427.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF038598 mRNA. Translation: AAB92621.1.
AE013599 Genomic DNA. Translation: AAF58566.1.
AY113465 mRNA. Translation: AAM29470.1.
RefSeqiNP_725106.1. NM_165866.3.
UniGeneiDm.19912.

3D structure databases

ProteinModelPortaliO44437.
SMRiO44437. Positions 1-84.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62087. 5 interactions.
DIPiDIP-19470N.
IntActiO44437. 26 interactions.
MINTiMINT-306370.
STRINGi7227.FBpp0087094.

Proteomic databases

PaxDbiO44437.
PRIDEiO44437.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087986; FBpp0087094; FBgn0023167.
GeneIDi36306.
KEGGidme:Dmel_CG8427.

Organism-specific databases

CTDi36306.
FlyBaseiFBgn0023167. SmD3.

Phylogenomic databases

eggNOGiKOG3172. Eukaryota.
COG1958. LUCA.
GeneTreeiENSGT00610000086153.
InParanoidiO44437.
KOiK11088.
OMAiEGHVVTC.
OrthoDBiEOG7VTDQF.
PhylomeDBiO44437.

Enzyme and pathway databases

ReactomeiR-DME-72163. mRNA Splicing - Major Pathway.
R-DME-72165. mRNA Splicing - Minor Pathway.

Miscellaneous databases

GenomeRNAii36306.
PROiO44437.

Gene expression databases

BgeeiO44437.
GenevisibleiO44437. DM.

Family and domain databases

InterProiIPR027141. LSm4/Sm_D1/D3.
IPR010920. LSM_dom.
IPR001163. LSM_dom_euk/arc.
[Graphical view]
PANTHERiPTHR23338. PTHR23338. 1 hit.
PfamiPF01423. LSM. 1 hit.
[Graphical view]
SMARTiSM00651. Sm. 1 hit.
[Graphical view]
SUPFAMiSSF50182. SSF50182. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The Drosophila gene for antizyme requires ribosomal frameshifting for expression and contains an intronic gene for snRNP Sm D3 on the opposite strand."
    Ivanov I.P., Simin K., Letsou A., Atkins J.F., Gesteland R.F.
    Mol. Cell. Biol. 18:1553-1561(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Embryo.
  2. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  3. Cited for: GENOME REANNOTATION.
    Strain: Berkeley.
  4. "A Drosophila full-length cDNA resource."
    Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A., Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M., Celniker S.E.
    Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  5. "Evolution of an RNP assembly system: a minimal SMN complex facilitates formation of UsnRNPs in Drosophila melanogaster."
    Kroiss M., Schultz J., Wiesner J., Chari A., Sickmann A., Fischer U.
    Proc. Natl. Acad. Sci. U.S.A. 105:10045-10050(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH THE SMN COMPLEX.
  6. "Sm protein methylation is dispensable for snRNP assembly in Drosophila melanogaster."
    Gonsalvez G.B., Praveen K., Hicks A.J., Tian L., Matera A.G.
    RNA 14:878-887(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: METHYLATION.

Entry informationi

Entry nameiSMD3_DROME
AccessioniPrimary (citable) accession number: O44437
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: June 1, 1998
Last modified: June 8, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.