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Protein

GLH-binding kinase 1

Gene

kgb-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Mitogen-activated protein kinase which is an essential component of the JNK pathway composed of mlk-1, mek-1 and kgb-1 (PubMed:15116070, PubMed:22554143, PubMed:23352664). Phosphorylates the transcription factor fos-1 which prevents fos-1 dimerization and promoter binding and results in activation of target genes including F53A9.2/kreg-1 and lys-3/kreg-2 (PubMed:23437011). Phosphorylates jun-1 and activates the AP-1 transcription factor which is a heterodimer of jun-1 and fos-1 (PubMed:23352664). Phosphorylates glh-1 in vitro which may play a role in controlling glh-1 protein levels in the germline by targeting it for degradation by the proteasome (PubMed:17699606). Required for oogenesis and probably also for spermatogenesis (PubMed:12435362). Involved in the response to environmental stress such as heavy metals, infection and protein folding stress in an age-dependent manner (PubMed:15116070, PubMed:22554143). In larvae, has a protective role which becomes detrimental in adults (PubMed:22554143). May control susceptibility to infection, heavy metal stress and premature lethality by regulating daf-16 cellular localization (PubMed:22554143). Involved in the transcriptional response to bacterial pore-forming toxins and to fasting (PubMed:15256590, PubMed:21408619, PubMed:23352664). Required for fasting-induced longevity (PubMed:23352664). Involved in axon regeneration after injury downstream of tyrosine receptor svh-2 (PubMed:21670305, PubMed:22388962).10 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.1 Publication

Cofactori

Mg2+By similarity

Enzyme regulationi

Activated by mek-1 mediated phosphorylation. No differences in basal activation between larvae and adults (PubMed:22554143). Inhibited by phosphatase vhp-1 (PubMed:15116070).2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei67ATPPROSITE-ProRule annotation1
Active sitei164Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 52ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DEAD/H-box RNA helicase binding Source: WormBase
  • JUN kinase activity Source: WormBase
  • protein C-terminus binding Source: WormBase
  • protein kinase activity Source: UniProtKB
  • protein serine/threonine kinase activity Source: WormBase
  • RNA polymerase II repressing transcription factor binding Source: WormBase

GO - Biological processi

  • activation of MAPK activity Source: UniProtKB
  • cellular response to arsenite ion Source: UniProtKB
  • cellular response to toxic substance Source: UniProtKB
  • defense response to Gram-negative bacterium Source: UniProtKB
  • determination of adult lifespan Source: UniProtKB
  • JUN phosphorylation Source: WormBase
  • male meiotic nuclear division Source: WormBase
  • negative regulation of defense response to bacterium Source: UniProtKB
  • negative regulation of gene expression Source: UniProtKB
  • negative regulation of protein homooligomerization Source: WormBase
  • negative regulation of protein localization to nucleus Source: UniProtKB
  • negative regulation of RNA polymerase II regulatory region sequence-specific DNA binding Source: WormBase
  • oocyte development Source: WormBase
  • peptidyl-threonine phosphorylation Source: WormBase
  • positive regulation of gene expression Source: UniProtKB
  • positive regulation of RNA splicing Source: UniProtKB
  • regulation of protein localization to nucleus Source: UniProtKB
  • reproduction Source: WormBase
  • response to cadmium ion Source: UniProtKB
  • response to copper ion Source: WormBase
  • response to endoplasmic reticulum stress Source: WormBase
  • response to nematicide Source: UniProtKB
  • response to starvation Source: UniProtKB
  • response to unfolded protein Source: UniProtKB
  • spermatogenesis Source: UniProtKB-KW
  • stress response to copper ion Source: WormBase

Keywordsi

Molecular functionKinase, Serine/threonine-protein kinase, Transferase
Biological processDifferentiation, Oogenesis, Spermatogenesis, Stress response
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

SignaLinkiO44408

Names & Taxonomyi

Protein namesi
Recommended name:
GLH-binding kinase 11 Publication (EC:2.7.11.241 Publication)
Gene namesi
Name:kgb-1Imported
ORF Names:T07A9.3Imported
OrganismiCaenorhabditis elegansImported
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiT07A9.3 ; CE29466 ; WBGene00002187 ; kgb-1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Temperature-sensitive sterility in both hermaphrodites and males which, in hermaphrodites, is associated with disorganized gonads and with impaired production of endomitotic oocytes, likely due to a defect in oocyte maturation which often results in vulva protrusion (PubMed:12435362). Both hermaphrodites and males produce sperm but it is defective (PubMed:12435362). In addition, the formation of P granules is disrupted in later stage of oogenesis and the protein levels of glh-1, a component of the P granules, are increased (PubMed:12435362, PubMed:17699606). Old adults are bloated and move more slowly (PubMed:17699606). Hypersensitivity and impaired up-regulation of several genes in response to heavy metals (Cu2+ and Cd2+) and to bacterial pore-forming toxins exposure (PubMed:15256590, PubMed:15116070, PubMed:21408619, PubMed:23437011). RNAi knockdown at different developmental stages shows age-dependent defects: larvae are hypersensitive to cadmium and protein folding stress and have a reduced survival capacity but these effects are not observed in adults (PubMed:22554143). Impaired fasting-induced longevity (PubMed:23352664). RNAi knockdown in adults results in increased daf-16 nuclear localization in intestinal cells but not in response to fasting (PubMed:22554143, PubMed:23352664). Mutants have impaired axon regeneration (PubMed:21670305).9 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi67K → N: Loss of activity. 1 Publication1
Mutagenesisi198S → A: Loss of activity. 1 Publication1
Mutagenesisi200Y → F: Loss of activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004333011 – 390GLH-binding kinase 1Add BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei198Phosphoserine2 Publications1
Modified residuei200Phosphotyrosine2 Publications1

Post-translational modificationi

May be phosphorylated by mek-1 on Ser-198 and/or Tyr-200 (PubMed:15116070). Phosphorylation is induced upon Cu2+ and arsenite-mediated cell stimulation and by fasting (PubMed:15116070, PubMed:23352664).1 Publication1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO44408
PaxDbiO44408
PeptideAtlasiO44408

PTM databases

iPTMnetiO44408

Expressioni

Tissue specificityi

Expressed in somatic and germline tissues.1 Publication

Inductioni

By B.thuringiensis pore-forming toxin Cry5B.1 Publication

Gene expression databases

BgeeiWBGene00002187

Interactioni

Subunit structurei

Interacts with glh-1, glh-2 (via C-terminus), glh-3 (via C-terminus) and glh-4 (via C-terminus) (PubMed:12435362, PubMed:17699606). Interacts with csn-5; the interaction may prevent glh-1 degradation induced by kgb-1 (PubMed:17699606). Interacts with fos-1 (PubMed:23437011).3 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • DEAD/H-box RNA helicase binding Source: WormBase
  • protein C-terminus binding Source: WormBase
  • RNA polymerase II repressing transcription factor binding Source: WormBase

Protein-protein interaction databases

DIPiDIP-24645N
IntActiO44408, 6 interactors
STRINGi6239.T07A9.3

Structurei

3D structure databases

ProteinModelPortaliO44408
SMRiO44408
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini38 – 338Protein kinasePROSITE-ProRule annotationAdd BLAST301

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0665 Eukaryota
ENOG410XSHI LUCA
GeneTreeiENSGT00550000074271
HOGENOMiHOG000233024
InParanoidiO44408
KOiK04440
OMAiFVYQSLC
OrthoDBiEOG091G08OW
PhylomeDBiO44408

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR003527 MAP_kinase_CS
IPR008351 MAPK_JNK
IPR000719 Prot_kinase_dom
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01772 JNKMAPKINASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS01351 MAPK, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

O44408-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEVDLPVHNE YDASRFHQVT IRDPIAGADS TFTIPTRYVN LSFLNAGAQG
60 70 80 90 100
TVVMADDLVT TQRVAIKKMQ QPFVMTMSAK RAYREFILLT TIKHPNIIRL
110 120 130 140 150
LNAFTPDTSL STFREVYLVM ELMTHNLHEV IHRLRLDHKT LSFFVYQSLC
160 170 180 190 200
AIKHLHNSGV IHRDLKPSNI VVNDRCVLKV LDFGLARKKN VDTSMRMSDY
210 220 230 240 250
VVTRYYRAPE VILGLPYSEK VDIWSVGCIF AEMINHTVLF PGKDRIDQWT
260 270 280 290 300
KIYSVLGTPD DHFISQLGQS AAMYVRSLPR HQARAFSEIV PDTNFLPETE
310 320 330 340 350
NPRVHLTPHV ARDLLFNMLK INPEERYSVE DALNHPYVKL WFKDDEVNAP
360 370 380 390
ASENRYDQEI DFADKTLIEW KELIFNEVQR YQADHDIFTG
Length:390
Mass (Da):45,157
Last modified:December 1, 2001 - v2
Checksum:iB3F09B5229C31A53
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FO081716 Genomic DNA Translation: CCD73974.1
RefSeqiNP_499922.1, NM_067521.4
UniGeneiCel.7426

Genome annotation databases

EnsemblMetazoaiT07A9.3; T07A9.3; WBGene00002187
GeneIDi176866
KEGGicel:CELE_T07A9.3
UCSCiT07A9.3 c. elegans

Similar proteinsi

Entry informationi

Entry nameiKGB1_CAEEL
AccessioniPrimary (citable) accession number: O44408
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 24, 2015
Last sequence update: December 1, 2001
Last modified: March 28, 2018
This is version 155 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. SIMILARITY comments
    Index of protein domains and families
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