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Protein

Integrin alpha-PS3

Gene

scb

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Integrin alpha-PS3/beta-PS is a receptor for laminin. Also binds to wb. Important during embryogenesis for the development of the trachea, dorsal vessel and salivary gland, as well as for dorsal closure. Required for short-term memory processes. Minor involvement in the establishment of the oocyte anterior-posterior length. Plays a role in timely border cell migration during oogenesis, probably mediated by JNK signaling. Integrin alpha-PS3/Itgbn is required for effective phagocytosis of apoptotic cells during embryonic development and for the phagocytic elimination of S.aureus by mediating the binding of S.aureus peptidoglycan to larval hemocytes, which probably activates a signaling pathway involving Rac1 and Rac2. Integrin alpha-PS3/Itgbn also regulates Fak activity during neuromuscular junction (NMJ) growth and is required for its activation in presynapsis of NMJs. Seems to be dispensable for major morphogenetic processes.5 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: FlyBase
  • protein heterodimerization activity Source: FlyBase
  • receptor activity Source: FlyBase

GO - Biological processi

  • apoptotic cell clearance Source: FlyBase
  • axon guidance Source: FlyBase
  • behavioral response to ethanol Source: FlyBase
  • calcium-dependent cell-cell adhesion via plasma membrane cell adhesion molecules Source: FlyBase
  • calcium-dependent cell-matrix adhesion Source: FlyBase
  • cell adhesion Source: FlyBase
  • cell adhesion mediated by integrin Source: FlyBase
  • cell-matrix adhesion Source: FlyBase
  • cell migration Source: FlyBase
  • dorsal closure Source: FlyBase
  • dorsal trunk growth, open tracheal system Source: FlyBase
  • heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules Source: FlyBase
  • integrin-mediated signaling pathway Source: UniProtKB-KW
  • larval heart development Source: FlyBase
  • learning or memory Source: FlyBase
  • locomotion Source: FlyBase
  • memory Source: FlyBase
  • midgut development Source: FlyBase
  • negative regulation of cell migration Source: UniProtKB
  • negative regulation of synaptic growth at neuromuscular junction Source: FlyBase
  • olfactory learning Source: FlyBase
  • oocyte growth Source: UniProtKB
  • pericardium morphogenesis Source: FlyBase
  • phagocytosis Source: FlyBase
  • salivary gland development Source: FlyBase
  • short-term memory Source: FlyBase
  • wound healing Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Integrin, Receptor

Keywords - Biological processi

Behavior, Cell adhesion, Differentiation, Oogenesis, Phagocytosis

Enzyme and pathway databases

ReactomeiR-DME-216083. Integrin cell surface interactions.
R-DME-3000170. Syndecan interactions.
R-DME-445144. Signal transduction by L1.
R-DME-445355. Smooth Muscle Contraction.
R-DME-6798695. Neutrophil degranulation.

Names & Taxonomyi

Protein namesi
Recommended name:
Integrin alpha-PS3
Alternative name(s):
Position-specific antigen subunit alpha-3
Short name:
Protein scab
Protein volado
Cleaved into the following 2 chains:
Gene namesi
Name:scb
Synonyms:alphaPS3, Vol
ORF Names:CG8095
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0003328. scb.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 1054ExtracellularSequence analysisAdd BLAST1030
Transmembranei1055 – 1075HelicalSequence analysisAdd BLAST21
Topological domaini1076 – 1115CytoplasmicSequence analysisAdd BLAST40

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • basal plasma membrane Source: UniProtKB
  • cytoplasm Source: UniProtKB
  • focal adhesion Source: FlyBase
  • integral component of membrane Source: FlyBase
  • integrin complex Source: FlyBase
  • lateral plasma membrane Source: UniProtKB-SubCell
  • plasma membrane Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Disruption phenotypei

Flies display impaired olfactory memories within 3 min of training. Mutant embryos show reduced level of phagocytosis.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001632625 – 1115Integrin alpha-PS3Add BLAST1091
ChainiPRO_000001632725 – ?929Integrin alpha-PS3 heavy chainSequence analysisAdd BLAST905
ChainiPRO_0000016328?930 – 1115Integrin alpha-PS3 light chainSequence analysisAdd BLAST186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi82N-linked (GlcNAc...)2 Publications1
Glycosylationi166N-linked (GlcNAc...)Sequence analysis1
Glycosylationi438N-linked (GlcNAc...)Sequence analysis1
Glycosylationi696N-linked (GlcNAc...)Sequence analysis1
Glycosylationi845N-linked (GlcNAc...)Sequence analysis1
Glycosylationi868N-linked (GlcNAc...)Sequence analysis1
Glycosylationi964N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO44386.
PRIDEiO44386.

Expressioni

Tissue specificityi

Expressed in embryonic and larval hemocytes (at protein level). Expressed in tissues undergoing invagination, tissue movement and morphogenesis such as salivary gland, trachea, midgut endoderm, dorsal vessel, midline of the ventral nerve cord, amnioserosa and the amnioproctodeal invagination. Expressed in the mushroom body neuropil, brain areas that contain mushroom body processes in synaptic contact with other neurons. In egg chambers, expressed in border cells, in stretch cells and in dorsal appendage primordia.5 Publications

Developmental stagei

Expressed throughout development. Highest expression is observed in pupae (at protein level). During oogenesis, expressed from stage 10B onwards.2 Publications

Gene expression databases

BgeeiFBgn0003328.
GenevisibleiO44386. DM.

Interactioni

Subunit structurei

Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of a heavy and a light chain linked by a disulfide bond. Interacts with mys/beta-PS and Itgbn.3 Publications

GO - Molecular functioni

  • cell adhesion molecule binding Source: FlyBase
  • protein heterodimerization activity Source: FlyBase

Protein-protein interaction databases

BioGridi62429. 4 interactors.
IntActiO44386. 1 interactor.
MINTiMINT-1552140.
STRINGi7227.FBpp0086501.

Structurei

3D structure databases

ProteinModelPortaliO44386.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati39 – 99FG-GAP 1PROSITE-ProRule annotationAdd BLAST61
Repeati113 – 174FG-GAP 2PROSITE-ProRule annotationAdd BLAST62
Repeati193 – 246FG-GAP 3PROSITE-ProRule annotationAdd BLAST54
Repeati278 – 335FG-GAP 4PROSITE-ProRule annotationAdd BLAST58
Repeati336 – 397FG-GAP 5PROSITE-ProRule annotationAdd BLAST62
Repeati398 – 453FG-GAP 6PROSITE-ProRule annotationAdd BLAST56
Repeati460 – 522FG-GAP 7PROSITE-ProRule annotationAdd BLAST63

Sequence similaritiesi

Belongs to the integrin alpha chain family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IPB1. Eukaryota.
ENOG4111GN2. LUCA.
GeneTreeiENSGT00760000118782.
InParanoidiO44386.
OMAiQHVTRIS.
OrthoDBiEOG091G0B2Y.
PhylomeDBiO44386.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 7 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform B (identifier: O44386-1) [UniParc]FASTAAdd to basket
Also known as: Vol-L

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNAESTMFPH IFLALLALIS HIEAFNFMPR PSRVINSPKH LKFHINQTRS
60 70 80 90 100
SYFGYTLVIR QTSIIVGAPR AQSTLESQRT INETGAIYRC SLTNGVCSPY
110 120 130 140 150
VLDSRGNVDA PYSEYTFDSE RKDFQWLGGS MDGGTKDTDK LLVCAPRFYA
160 170 180 190 200
PSSRDNHLHG VCYWVNNTVA STPQHVTRIS PLRLKSEQVK EEDNGNKASF
210 220 230 240 250
FYIMGELGLS AHVADDNTKF LIGAPGINTW RGSVILYRQV DPVDNPTASR
260 270 280 290 300
RDTSKALRRT YRDVDSNDYT PEHYAPEIPT PGLWGQEEDS YFGYAVSSGF
310 320 330 340 350
FDSSNPTKLL YVATAPQANK QSGEAYIFDV RGKSIHKYHV FRGEQFGEYF
360 370 380 390 400
GYSVLAEDLN GDGKTDVIVS APQHALEDSH DNGAIYVFIN KGFFNFERQI
410 420 430 440 450
LRSPVETMAR FGTALSRLGD INHDGYNDVA VGAPFAGNGT VFIYLGSENG
460 470 480 490 500
LRDQPSQRLD APSQQPSKYG SHMFGHGLSR GSDIDGNGFN DFAIGAPNAE
510 520 530 540 550
AVYLYRAYPV VKVHATVKSE SREIKPEQEK VKITACYRLS TTSTDKLVQE
560 570 580 590 600
QELAIRIAMD KQLKRVKFTQ TQTNEISFKV NANFGEQCRD FETQVRYSEK
610 620 630 640 650
DIFTPIDLEM HYELTKKVPD SEEFCETCAI VDPTEPKVST QNIIFSTGCA
660 670 680 690 700
TDVCTADLQL RSKDVSPTYI LGSADTLRLN YEITNIGETA YLPQFNVTST
710 720 730 740 750
SRLAFAQVPG NCKVVDAVMV CDLNRGRPLA KGDTDSVTIS FDVSQLSGQS
760 770 780 790 800
LIIHAEVFST GYEQNPTDNR QTNVIGLKEF TEIDASGGQT NSQIDLEHYS
810 820 830 840 850
NSAEIVNNYE IKSNGPSVIE QLTVSFYIPI AYKVAGSTAI IPIINVTSLK
860 870 880 890 900
MQASYDSQLL SIDLYDQNNT MLVVDPVEVT TTLSGGLERT VITQNRQSYD
910 920 930 940 950
IHTSGHVHQT MEVLDTSMVA TASMSRKRRD LKALTANREQ YARISNVKAH
960 970 980 990 1000
DLLSDDFKGK LPVNRTIVFN CRDPEMTICV RAEMRVHFRP EKSINLNMRY
1010 1020 1030 1040 1050
SVDLNEVNAI LVDPWEYFVI LTDLKLQKKG DPTSTSFSIN RRIEPNIISK
1060 1070 1080 1090 1100
HQETGLPIWI IIVSVIGGLL LLSAISYLLY KFGFFNRTKK DELDRLVQQN
1110
PVEPEAENLN SGGNN
Length:1,115
Mass (Da):124,545
Last modified:April 27, 2001 - v2
Checksum:iB3DFB52212E84185
GO
Isoform A (identifier: O44386-2) [UniParc]FASTAAdd to basket
Also known as: Vol-S

The sequence of this isoform differs from the canonical sequence as follows:
     1-63: MNAESTMFPH...GYTLVIRQTS → MVGQDRDFWA...GFTMSLRPNG

Show »
Length:1,115
Mass (Da):124,336
Checksum:i76E6B66B380E808D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti630I → V in AAC38853 (PubMed:9461212).Curated1
Sequence conflicti630I → V in AAC38854 (PubMed:9461212).Curated1
Sequence conflicti664D → N in AAC38853 (PubMed:9461212).Curated1
Sequence conflicti664D → N in AAC38854 (PubMed:9461212).Curated1
Sequence conflicti753I → S in AAC38853 (PubMed:9461212).Curated1
Sequence conflicti753I → S in AAC38854 (PubMed:9461212).Curated1
Sequence conflicti792S → R in AAC38853 (PubMed:9461212).Curated1
Sequence conflicti792S → R in AAC38854 (PubMed:9461212).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0027401 – 63MNAES…IRQTS → MVGQDRDFWALLVLGLWCLS SHCNAFNLSPLPNRQILDPQ FATNVPKVRASYFGFTMSLR PNG in isoform A. 2 PublicationsAdd BLAST63

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76605 mRNA. Translation: AAC04505.1.
AF034199 mRNA. Translation: AAC38853.1.
AF034200 mRNA. Translation: AAC38854.1.
AE013599 Genomic DNA. Translation: AAF58155.1.
AE013599 Genomic DNA. Translation: AAF58156.1.
BT015245 mRNA. Translation: AAT94474.1.
BT021944 mRNA. Translation: AAX51649.1.
PIRiT09403.
T09433.
RefSeqiNP_523750.2. NM_079026.3. [O44386-1]
NP_725445.1. NM_166083.2. [O44386-2]
UniGeneiDm.636.

Genome annotation databases

EnsemblMetazoaiFBtr0087369; FBpp0086501; FBgn0003328. [O44386-1]
GeneIDi36692.
KEGGidme:Dmel_CG8095.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U76605 mRNA. Translation: AAC04505.1.
AF034199 mRNA. Translation: AAC38853.1.
AF034200 mRNA. Translation: AAC38854.1.
AE013599 Genomic DNA. Translation: AAF58155.1.
AE013599 Genomic DNA. Translation: AAF58156.1.
BT015245 mRNA. Translation: AAT94474.1.
BT021944 mRNA. Translation: AAX51649.1.
PIRiT09403.
T09433.
RefSeqiNP_523750.2. NM_079026.3. [O44386-1]
NP_725445.1. NM_166083.2. [O44386-2]
UniGeneiDm.636.

3D structure databases

ProteinModelPortaliO44386.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi62429. 4 interactors.
IntActiO44386. 1 interactor.
MINTiMINT-1552140.
STRINGi7227.FBpp0086501.

Proteomic databases

PaxDbiO44386.
PRIDEiO44386.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0087369; FBpp0086501; FBgn0003328. [O44386-1]
GeneIDi36692.
KEGGidme:Dmel_CG8095.

Organism-specific databases

CTDi20242.
FlyBaseiFBgn0003328. scb.

Phylogenomic databases

eggNOGiENOG410IPB1. Eukaryota.
ENOG4111GN2. LUCA.
GeneTreeiENSGT00760000118782.
InParanoidiO44386.
OMAiQHVTRIS.
OrthoDBiEOG091G0B2Y.
PhylomeDBiO44386.

Enzyme and pathway databases

ReactomeiR-DME-216083. Integrin cell surface interactions.
R-DME-3000170. Syndecan interactions.
R-DME-445144. Signal transduction by L1.
R-DME-445355. Smooth Muscle Contraction.
R-DME-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiscrib. fly.
GenomeRNAii36692.
PROiO44386.

Gene expression databases

BgeeiFBgn0003328.
GenevisibleiO44386. DM.

Family and domain databases

InterProiIPR013519. Int_alpha_beta-p.
IPR000413. Integrin_alpha.
IPR013649. Integrin_alpha-2.
IPR018184. Integrin_alpha_C_CS.
IPR032695. Integrin_dom.
[Graphical view]
PfamiPF08441. Integrin_alpha2. 1 hit.
[Graphical view]
PRINTSiPR01185. INTEGRINA.
SMARTiSM00191. Int_alpha. 7 hits.
[Graphical view]
SUPFAMiSSF69179. SSF69179. 2 hits.
PROSITEiPS51470. FG_GAP. 7 hits.
PS00242. INTEGRIN_ALPHA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiITA3_DROME
AccessioniPrimary (citable) accession number: O44386
Secondary accession number(s): O44387
, O46186, Q58CK3, Q6AWK3, Q9V7A3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 30, 2016
This is version 160 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.