O44220 (C12B1_DROAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome P450 12b1, mitochondrial
EC=1.14.-.-

Alternative name(s):
CYPXIIB1

Gene names

Name:

Cyp12b1

Organism

Drosophila acanthoptera (Fruit fly)

Taxonomic identifier

51166 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Hexapoda › Insecta › Dicondylia › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Eremoneura › Cyclorrhapha › Schizophora › Acalyptratae › Ephydroidea › Drosophilidae › Drosophilinae › Drosophilini › Drosophilina › Drosophiliti › Drosophila › Drosophila › nannoptera group

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Probably involved in steroid hormones biosynthesis.
Cofactor	Heme group By similarity.
Subcellular location	Mitochondrion Probable.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	Heme Iron Metal-binding
Molecular function	Monooxygenase Oxidoreductase
Gene Ontology (GO)
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion By similarity
	Chain	? – 532		Cytochrome P450 12b1, mitochondrial		PRO_0000003610
Sites
	Metal binding	480	1	Iron (heme axial ligand) Potential

Sequences

	Sequence		Length	Mass (Da)
	O44220 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 2E94D7FC66FDF569	FASTA	532	62,031
	10 20 30 40 50 60 MWKFAIHSQQ PFCWQQLCNR RHLYVGNVQQ QTHLELLDAA PTRSDDEWLQ AKPYEKVPGP 70 80 90 100 110 120 GTWQVLSYFL PGGKQYNTNL IQMNRRMREW YGDIYRFPGL MGKQDVIFTY NPNDFELTYR 130 140 150 160 170 180 NEGVWPIRIG LESFTYYRKV HRPEVFGSIG GLVSEQGKDW AHIRNKVNPV QMRVQNVRQN 190 200 210 220 230 240 LPQIDQISRE FVDKLDTLRD PVTHILNDNF HEQLKMWAFE SISFVALNTR MGLLSDRPDP 250 260 270 280 290 300 NAARLAEHMT DFFNYSFKYD VQPSIWPYYK TPGFKKFLQT YDKITEITTA YIDEAIKRFE 310 320 330 340 350 360 IEKDSGNECV LQQLLSLNKK VAVVMAMYML MAGIDTTSSA FVTILYHLAR NPHKQRQLHR 370 380 390 400 410 420 ERRRILPDSD EPLTPENTKN MPYLRACIKE CMRITSITPG NFRIATKDLV LSGYRVPRGE 430 440 450 460 470 480 GVLMGVLELS NSEKYFGQSG QFMPERWLKA DTDPDVKACP AARSRNPFVY LAFGFGPRTC 490 500 510 520 530 IGKRIAELEM ETLLTRLLRR YQVSWLAEMP LQYESNIILS PHGIYVQVRA AC

References

[1]	"Isolation and sequence analysis of cytochrome P450 12B1: the first mitochondrial insect P450 with homology to 1 alpha,25 dihydroxy-D3 24-hydroxylase." Danielson P.B., Fogleman J.C. Insect Biochem. Mol. Biol. 27:595-604(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: A584.2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U78485 mRNA. Translation: AAB88725.1.
3D structure databases
ProteinModelPortal	O44220.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0022933. Daca\Cyp12b1.
Family and domain databases
Gene3D	1.10.630.10. 1 hit.
InterPro	IPR001128. Cyt_P450. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
SUPFAM	SSF48264. Cytochrome_P450. 1 hit.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C12B1_DROAC

Accession

Primary (citable) accession number: O44220

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project