O44119 (TPM_HOMAM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Tropomyosin

Alternative name(s):
Allergen=Hom a 1

Gene names

Name:

TM1

Organism

Homarus americanus (American lobster)

Taxonomic identifier

6706 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Panarthropoda › Arthropoda › Mandibulata › Pancrustacea › Crustacea › Malacostraca › Eumalacostraca › Eucarida › Decapoda › Pleocyemata › Astacidea › Nephropoidea › Nephropidae › Homarus

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tropomyosin, in association with the troponin complex, plays a central role in the calcium dependent regulation of muscle contraction.
Subunit structure	Homodimer By similarity.
Domain	The molecule is in a coiled coil structure that is formed by 2 polypeptide chains. The sequence exhibits a prominent seven-residues periodicity.
Allergenic properties	Causes an allergic reaction in human.
Sequence similarities	Belongs to the tropomyosin family.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Disease	Allergen
Domain	Coiled coil Repeat
Gene Ontology (GO)
None. [Check GOA]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Slow muscle 1 (identifier: O44119-1)

Also known as: STM1;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Fast muscle type (identifier: O44119-2)

Also known as: FTM;

The sequence of this isoform differs from the canonical sequence as follows:
     39-79: TEEEIRITHK...KLEEKEKALQ → SEEEVHNLQK...QLEEKDKALS

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Tropomyosin		PRO_0000205674
Regions
	Coiled coil	1 – 284	284
Natural variations
	Alternative sequence	39 – 79	41	TEEEI…EKALQ → SEEEVHNLQKRMQQLENDLD QVQESLLKANTQLEEKDKAL S in isoform Fast muscle type.		VSP_006615

Sequences

	Sequence		Length	Mass (Da)
	Isoform Slow muscle 1 (STM1) [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C238DDB4E693243A	FASTA	284	32,907
	10 20 30 40 50 60 MDAIKKKMQA MKLEKDNAMD RADTLEQQNK EANIRAEKTE EEIRITHKKM QQVENELDQV 70 80 90 100 110 120 QEQLSLANTK LEEKEKALQN AEGEVAALNR RIQLLEEDLE RSEERLNTAT TKLAEASQAA 130 140 150 160 170 180 DESERMRKVL ENRSLSDEER MDALENQLKE ARFLAEEADR KYDEVARKLA MVEADLERAE 190 200 210 220 230 240 ERAETGESKI VELEEELRVV GNNLKSLEVS EEKANQREEA YKEQIKTLAN KLKAAEARAE 250 260 270 280 FAERSVQKLQ KEVDRLEDEL VNEKEKYKSI TDELDQTFSE LSGY
	Isoform Fast muscle type (FTM) [UniParc]. Checksum: 3786F60C224BC359	FASTA	284	32,836
	10 20 30 40 50 60 MDAIKKKMQA MKLEKDNAMD RADTLEQQNK EANIRAEKSE EEVHNLQKRM QQLENDLDQV 70 80 90 100 110 120 QESLLKANTQ LEEKDKALSN AEGEVAALNR RIQLLEEDLE RSEERLNTAT TKLAEASQAA 130 140 150 160 170 180 DESERMRKVL ENRSLSDEER MDALENQLKE ARFLAEEADR KYDEVARKLA MVEADLERAE 190 200 210 220 230 240 ERAETGESKI VELEEELRVV GNNLKSLEVS EEKANQREEA YKEQIKTLAN KLKAAEARAE 250 260 270 280 FAERSVQKLQ KEVDRLEDEL VNEKEKYKSI TDELDQTFSE LSGY

References

[1]	"Cloning of tropomyosins from lobster (Homarus americanus) striated muscles: fast and slow isoforms may be generated from the same transcript." Mykles D.L., Cotton J.L.S., Taniguchi H., Sano K., Maeda Y. J. Muscle Res. Cell Motil. 19:105-115(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS FAST MUSCLE TYPE AND SLOW MUSCLE 1). Tissue: Skeletal muscle.
[2]	"Production and crystallization of lobster muscle tropomyosin expressed in Sf9 cells." Miegel A., Sano K., Yamamoto K., Maeda K., Maeda Y., Taniguchi H., Yao M., Wakatsuki S. FEBS Lett. 394:201-205(1996) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (3.0 ANGSTROMS).

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF034953 mRNA. Translation: AAC48287.1. AF034954 mRNA. Translation: AAC48288.1.
PIR	S74168.
3D structure databases
ProteinModelPortal	O44119.
SMR	O44119. Positions 1-283.
ModBase	Search...
Protein family/group databases
Allergome	3320. Hom a 1.0101. 3321. Hom a 1.0102. 410. Hom a 1.
Proteomic databases
PRIDE	O44119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR000533. Tropomyosin. [Graphical view]
Pfam	PF00261. Tropomyosin. 1 hit. [Graphical view]
PRINTS	PR00194. TROPOMYOSIN.
PROSITE	PS00326. TROPOMYOSIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TPM_HOMAM

Accession

Primary (citable) accession number: O44119
Secondary accession number(s): O44120

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	June 1, 1998
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)