O44111 (URIC_DROSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uricase
EC=1.7.3.3

Alternative name(s):
Urate oxidase

Gene names

Name:	Uro
Synonyms:	UO

Organism

Drosophila subobscura (Fruit fly)

Taxonomic identifier

7241 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	339 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the oxidation of uric acid to 5-hydroxyisourate, which is further processed to form (S)-allantoin.
Catalytic activity	Urate + O2 + H2O = 5-hydroxyisourate + H2O2.
Pathway	Purine metabolism; urate degradation; (S)-allantoin from urate: step 1/3.
Subcellular location	Peroxisome.
Developmental stage	Third instar larvae and adult.
Sequence similarities	Belongs to the uricase family.

Ontologies

Keywords
Biological process	Purine metabolism
Cellular component	Peroxisome
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	purine base metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	peroxisome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	urate oxidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 339	›339	Uricase		PRO_0000165993
Regions
	Region	78 – 79	2	Substrate binding By similarity
	Region	266 – 267	2	Substrate binding By similarity
	Motif	337 – 339	3	Microbody targeting signal Potential
Sites
	Active site	218	1	Charge relay system By similarity
	Active site	267	1	Charge relay system By similarity
	Binding site	218	1	Substrate By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	O44111 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 34439F6B6078DC6E	FASTA	339	38,437
	10 20 30 40 50 60 LRQPSSASNR TAASLDGQET PFQYEITDHG YGKDAVKVLH VSRKGPVHTI QEFEVGTHLK 70 80 90 100 110 120 LYSKKDYYQG NNSDIVATDS QKNTVYLLAK KYGIESPEKF ALLLGQHFLN KYSHVEEAHV 130 140 150 160 170 180 HVETYPWQRV CQEETKASTT VGQGSCNFSS IDNRSLHNHA FIFTPTALHY CDVVIXRQDP 190 200 210 220 230 240 KQTVITGIKG LRVLKTTQSS FVNFVNDEXR SLPDQYDRIF STVVDCSWEY SDTDTVNFSR 250 260 270 280 290 300 AWQQVKNIIL RNFAGDPQVG VSSPSVQHTL YLSEKQVLDV IPQVSVISMT MPNKHYFNFD 310 320 330 TKPFQQIVPG DNNEVFIPVD KPHGTIYAQL ARKNLNSHL

References

[1]	"The molecular clock revisited: the rate of synonymous vs. replacement change in Drosophila." Zeng L.-W., Comeron J.M., Chen B., Kreitman M. Genetica 102:369-382(1998) [PubMed: 9720289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF025816 mRNA. Translation: AAB87901.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
FlyBase	FBgn0021077. Dsub\Uro.
Family and domain databases
InterPro	IPR002042. Uricase. [Graphical view]
Gene3D	G3DSA:3.10.270.10. Uricase. 1 hit.
PANTHER	PTHR10395:SF1. Uricase. 1 hit.
Pfam	PF01014. Uricase. 2 hits. [Graphical view]
PIRSF	PIRSF000241. Urate_oxidase. 1 hit.
PRINTS	PR00093. URICASE.
TIGRFAMs	TIGR03383. Urate_oxi. 1 hit.
PROSITE	PS00366. URICASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

URIC_DROSU

Accession

Primary (citable) accession number: O44111

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	June 28, 2011
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Drosophila annotation project