Reviewed, UniProtKB/Swiss-Prot O44105 (G3P2_DROSU)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase 2
    EC=1.2.1.12
Alternative name(s):
    Glyceraldehyde-3-phosphate dehydrogenase II
      Short name=GAPDH II

Gene names

Name:

Gapdh2

Organism

Drosophila subobscura (Fruit fly)

Taxonomic identifier

7241 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	304 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Subunit structure	Homotetramer.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Glycolysis
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glycolysis Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – ›304	›304	Glyceraldehyde-3-phosphate dehydrogenase 2		PRO_0000145525
Regions
	Nucleotide binding	1 – 2	2	NAD By similarity
	Region	138 – 140	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	198 – 199	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	139	1	Nucleophile By similarity
	Binding site	22	1	NAD By similarity
	Binding site	67	1	NAD; via carbonyl oxygen By similarity
	Binding site	169	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	221	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	303	1	NAD By similarity
	Site	166	1	Activates thiol group during catalysis By similarity
Experimental info
	Non-terminal residue	1	1
	Non-terminal residue	304	1

Sequences

	Sequence		Length	Mass (Da)
	O44105-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 9BE9C70576089D1C	FASTA	304	32,176
	10 20 30 40 50 60 RIGRLVLRAA IDKGASVVAV NDPFIDVNYM VYLFKFDSTH GRFKGTVVAE GGFLVVNGQK 70 80 90 100 110 120 ITVFSERDPA NINWASAGAE YVVESTGVFT TIEKASTHLK GGAKKVVISA PSADAPMFVC 130 140 150 160 170 180 GVNLDAYKPD MKVVSNASCT TNCLAPLAKV INDNFEIVEG LMTTVHATTA TQKTVDGPSG 190 200 210 220 230 240 KLWRDGRGAA QNIIPAATGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV DLTVRLGKGA 250 260 270 280 290 300 SYDEIKAKVQ EAANGPLKGI LGYTDEEVVS TDFLSDTHSS VFDAKAGISL NDKFVKLISW YDNE

References

[1]	"The molecular clock revisited: the rate of synonymous vs. replacement change in Drosophila." Zeng L.-W., Comeron J.M., Chen B., Kreitman M. Genetica 102:369-382(1998) [PubMed: 9720289] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF025810 mRNA. Translation: AAB87895.1.
3D structure databases
HSSP	HSSP built from PDB template 1DSS based on UniProtKB P56649.
SMR	O44105. Positions 1-304.
ModBase	Search...
Organism-specific databases
FlyBase	FBgn0012937. Dsub\Gapdh2.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DHase. IPR006424. Glyceraldehyde-3-P_DHase_1. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000149. GAP_DH. 1 hit.
PRINTS	PR00078. G3PDHDRGNASE.
TIGRFAMs	TIGR01534. GAPDH-I. 1 hit.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3P2_DROSU

Accession

Primary (citable) accession number: O44105

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 1, 1998
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project