ID   G3P2_DROPS              Reviewed;         332 AA.
AC   O44104; Q29CT3;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2006, sequence version 2.
DT   16-JUN-2009, entry version 58.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2;
DE            EC=1.2.1.12;
DE   AltName: Full=Glyceraldehyde-3-phosphate dehydrogenase II;
DE            Short=GAPDH II;
GN   Name=Gapdh2; ORFNames=GA21397;
OS   Drosophila pseudoobscura pseudoobscura (Fruit fly).
OC   Eukaryota; Metazoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha;
OC   Ephydroidea; Drosophilidae; Drosophila; Sophophora.
OX   NCBI_TaxID=46245;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=MV2-25;
RX   PubMed=15632085; DOI=10.1101/gr.3059305;
RA   Richards S., Liu Y., Bettencourt B.R., Hradecky P., Letovsky S.,
RA   Nielsen R., Thornton K., Hubisz M.J., Chen R., Meisel R.P.,
RA   Couronne O., Hua S., Smith M.A., Zhang P., Liu J., Bussemaker H.J.,
RA   van Batenburg M.F., Howells S.L., Scherer S.E., Sodergren E.,
RA   Matthews B.B., Crosby M.A., Schroeder A.J., Ortiz-Barrientos D.,
RA   Rives C.M., Metzker M.L., Muzny D.M., Scott G., Steffen D.,
RA   Wheeler D.A., Worley K.C., Havlak P., Durbin K.J., Egan A., Gill R.,
RA   Hume J., Morgan M.B., Miner G., Hamilton C., Huang Y., Waldron L.,
RA   Verduzco D., Clerc-Blankenburg K.P., Dubchak I., Noor M.A.F.,
RA   Anderson W., White K.P., Clark A.G., Schaeffer S.W., Gelbart W.M.,
RA   Weinstock G.M., Gibbs R.A.;
RT   "Comparative genome sequencing of Drosophila pseudoobscura:
RT   chromosomal, gene, and cis-element evolution.";
RL   Genome Res. 15:1-18(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 11-314.
RX   MEDLINE=98386758; PubMed=9720289; DOI=10.1023/A:1017035109224;
RA   Zeng L.-W., Comeron J.M., Chen B., Kreitman M.;
RT   "The molecular clock revisited: the rate of synonymous vs. replacement
RT   change in Drosophila.";
RL   Genetica 102:369-382(1998).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EAL29271.1; Type=Erroneous gene model prediction;
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; CH475410; EAL29271.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; AF025809; AAB87894.1; -; mRNA.
DR   RefSeq; XP_001352353.1; -.
DR   HSSP; P56649; 1DSS.
DR   SMR; O44104; 2-332.
DR   GeneID; 4811785; -.
DR   KEGG; dpo:Dpse_GA21397; -.
DR   FlyBase; FBgn0012698; Dpse\Gapdh2.
DR   OMA; O44104; SRDDIEV.
DR   BRENDA; 1.2.1.12; 190795.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:EC.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DH_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    332       Glyceraldehyde-3-phosphate dehydrogenase
FT                                2.
FT                                /FTId=PRO_0000145524.
FT   NP_BIND      11     12       NAD (By similarity).
FT   REGION      148    150       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      208    209       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    149    149       Nucleophile (By similarity).
FT   BINDING      32     32       NAD (By similarity).
FT   BINDING      77     77       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     179    179       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     231    231       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     313    313       NAD (By similarity).
FT   SITE        176    176       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   332 AA;  35312 MW;  9E58BEB163B59983 CRC64;
     MSKIGINGFG RIGRLVLRAA IDKGASVVAV NDPFIDVNYM VYLFKFDSTH GRFKGTVAAE
     GGFLVVNGQK ITVFSERDPA NINWASAGAE YVVESTGVFT TIEKASTHLK GGAKKVVISA
     PSADAPMFVC GVNLDAYKPD MKVVSNASCT TNCLAPLAKV INDNFEIVEG LMTTVHATTA
     TQKTVDGPSG KLWRDGRGAA QNIIPAATGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV
     DLTVRLGKGA SYDEIKAKVQ EAANGPLKGI LGYTDEEVVS TDFLSDTHSS VFDAKAGISL
     NDKFVKLISW YDNEFGYSNR VIDLIKYMQS KD
//