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Protein

Glyceraldehyde-3-phosphate dehydrogenase 2

Gene

Gapdh2

Organism
Drosophila pseudoobscura pseudoobscura (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH.PROSITE-ProRule annotation

Pathwayi: glycolysis

This protein is involved in step 1 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. Glyceraldehyde-3-phosphate dehydrogenase 2 (Gapdh2), Glyceraldehyde-3-phosphate dehydrogenase (Dpse\GA21475)
  2. no protein annotated in this organism
  3. no protein annotated in this organism
  4. no protein annotated in this organism
  5. Pyruvate kinase (Dpse\GA26534), Pyruvate kinase (Dpse\GA20296), Pyruvate kinase (Dpse\GA15544), Pyruvate kinase (Dpse\GA26534), Pyruvate kinase (Dpse\GA26534)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei32 – 321NADBy similarity
Binding sitei77 – 771NAD; via carbonyl oxygenBy similarity
Active sitei149 – 1491NucleophilePROSITE-ProRule annotation
Sitei176 – 1761Activates thiol group during catalysisBy similarity
Binding sitei179 – 1791Glyceraldehyde 3-phosphateBy similarity
Binding sitei231 – 2311Glyceraldehyde 3-phosphateBy similarity
Binding sitei313 – 3131NADBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 122NADBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Names & Taxonomyi

Protein namesi
Recommended name:
Glyceraldehyde-3-phosphate dehydrogenase 2 (EC:1.2.1.12)
Alternative name(s):
Glyceraldehyde-3-phosphate dehydrogenase II
Short name:
GAPDH II
Gene namesi
Name:Gapdh2
ORF Names:GA21397
OrganismiDrosophila pseudoobscura pseudoobscura (Fruit fly)
Taxonomic identifieri46245 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000001819 Componenti: Partially assembled WGS sequence

Organism-specific databases

FlyBaseiFBgn0012698. Dpse\Gapdh2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 332332Glyceraldehyde-3-phosphate dehydrogenase 2PRO_0000145524Add
BLAST

Proteomic databases

PRIDEiO44104.

Interactioni

Subunit structurei

Homotetramer.

Structurei

3D structure databases

ProteinModelPortaliO44104.
SMRiO44104. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1503Glyceraldehyde 3-phosphate bindingBy similarity
Regioni208 – 2092Glyceraldehyde 3-phosphate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

InParanoidiO44104.
KOiK00134.
OMAiKWGEVGA.
PhylomeDBiO44104.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O44104-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKIGINGFG RIGRLVLRAA IDKGASVVAV NDPFIDVNYM VYLFKFDSTH
60 70 80 90 100
GRFKGTVAAE GGFLVVNGQK ITVFSERDPA NINWASAGAE YVVESTGVFT
110 120 130 140 150
TIEKASTHLK GGAKKVVISA PSADAPMFVC GVNLDAYKPD MKVVSNASCT
160 170 180 190 200
TNCLAPLAKV INDNFEIVEG LMTTVHATTA TQKTVDGPSG KLWRDGRGAA
210 220 230 240 250
QNIIPAATGA AKAVGKVIPA LNGKLTGMAF RVPTPNVSVV DLTVRLGKGA
260 270 280 290 300
SYDEIKAKVQ EAANGPLKGI LGYTDEEVVS TDFLSDTHSS VFDAKAGISL
310 320 330
NDKFVKLISW YDNEFGYSNR VIDLIKYMQS KD
Length:332
Mass (Da):35,312
Last modified:May 30, 2006 - v2
Checksum:i9E58BEB163B59983
GO

Sequence cautioni

The sequence EAL29271 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH475410 Genomic DNA. Translation: EAL29271.1. Sequence problems.
AF025809 mRNA. Translation: AAB87894.1.
RefSeqiXP_001352353.1. XM_001352317.3.

Genome annotation databases

EnsemblMetazoaiFBtr0286854; FBpp0285292; FBgn0012698.
GeneIDi4811785.
KEGGidpo:Dpse_GA21397.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CH475410 Genomic DNA. Translation: EAL29271.1. Sequence problems.
AF025809 mRNA. Translation: AAB87894.1.
RefSeqiXP_001352353.1. XM_001352317.3.

3D structure databases

ProteinModelPortaliO44104.
SMRiO44104. Positions 2-332.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiO44104.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0286854; FBpp0285292; FBgn0012698.
GeneIDi4811785.
KEGGidpo:Dpse_GA21397.

Organism-specific databases

FlyBaseiFBgn0012698. Dpse\Gapdh2.

Phylogenomic databases

InParanoidiO44104.
KOiK00134.
OMAiKWGEVGA.
PhylomeDBiO44104.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00184.

Miscellaneous databases

ChiTaRSiGapdh2. fly.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR020831. GlycerAld/Erythrose_P_DH.
IPR020830. GlycerAld_3-P_DH_AS.
IPR020829. GlycerAld_3-P_DH_cat.
IPR020828. GlycerAld_3-P_DH_NAD(P)-bd.
IPR006424. Glyceraldehyde-3-P_DH_1.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10836. PTHR10836. 1 hit.
PfamiPF02800. Gp_dh_C. 1 hit.
PF00044. Gp_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000149. GAP_DH. 1 hit.
PRINTSiPR00078. G3PDHDRGNASE.
SMARTiSM00846. Gp_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
TIGRFAMsiTIGR01534. GAPDH-I. 1 hit.
PROSITEiPS00071. GAPDH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiG3P2_DROPS
AccessioniPrimary (citable) accession number: O44104
Secondary accession number(s): Q29CT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 30, 2006
Last modified: September 7, 2016
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.