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Protein

H/ACA ribonucleoprotein complex subunit 4

Gene

Nop60B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis.3 Publications

Catalytic activityi

RNA uridine = RNA pseudouridine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei123NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • box H/ACA snoRNA 3'-end processing Source: GO_Central
  • cellular response to starvation Source: FlyBase
  • germ cell development Source: FlyBase
  • mRNA pseudouridine synthesis Source: GO_Central
  • neurogenesis Source: FlyBase
  • pseudouridine synthesis Source: FlyBase
  • ribosome biogenesis Source: FlyBase
  • rRNA processing Source: FlyBase
  • rRNA pseudouridine synthesis Source: GO_Central
  • snRNA pseudouridine synthesis Source: GO_Central
  • wing disc development Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Ribonucleoprotein

Keywords - Biological processi

Ribosome biogenesis, rRNA processing

Keywords - Ligandi

RNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 4 (EC:5.4.99.-)
Alternative name(s):
Nucleolar protein AT band 60B
Protein minifly
Gene namesi
Name:Nop60B
Synonyms:mfl
ORF Names:CG3333
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0259937. Nop60B.

Subcellular locationi

GO - Cellular componenti

  • box H/ACA snoRNP complex Source: GO_Central
  • nucleolus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219891 – 508H/ACA ribonucleoprotein complex subunit 4Add BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei442Phosphoserine1 Publication1
Modified residuei443Phosphothreonine1 Publication1
Modified residuei444Phosphoserine1 Publication1
Modified residuei445Phosphoserine1 Publication1
Modified residuei449Phosphothreonine1 Publication1
Modified residuei455Phosphoserine1 Publication1
Modified residuei458Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO44081.
PRIDEiO44081.

PTM databases

iPTMnetiO44081.

Expressioni

Developmental stagei

Isoform C is expressed both maternally and zygotically. Isoform A is expressed at the same level in adult females but at a much lower level in ovaries.1 Publication

Gene expression databases

BgeeiFBgn0259937.
ExpressionAtlasiO44081. baseline.
GenevisibleiO44081. DM.

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particle containing H/ACA-type snoRNAs (H/ACA snoRNPs).By similarity

Protein-protein interaction databases

BioGridi63449. 4 interactors.
DIPiDIP-20128N.
IntActiO44081. 3 interactors.
MINTiMINT-1030866.
STRINGi7227.FBpp0099583.

Structurei

3D structure databases

ProteinModelPortaliO44081.
SMRiO44081.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 369PUAPROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 16Poly-Lys9
Compositional biasi461 – 471Poly-LysAdd BLAST11
Compositional biasi489 – 499Poly-LysAdd BLAST11

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated
Contains 1 PUA domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2529. Eukaryota.
COG0130. LUCA.
GeneTreeiENSGT00510000047092.
InParanoidiO44081.
KOiK11131.
OMAiYVDYNAK.
OrthoDBiEOG091G079R.
PhylomeDBiO44081.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O44081-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW
60 70 80 90 100
PLLLKNFDKL NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS
110 120 130 140 150
HEVVAWIKKI LKVEKTGHSG TLDPKVTGCL IVCIDRATRL VKSQQSAGKE
160 170 180 190 200
YVAIFKLHGA VESVAKVRQG LEKLRGALFQ RPPLISAVKR QLRVRTVYDS
210 220 230 240 250
KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ MLELRRVRSG
260 270 280 290 300
IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK
310 320 330 340 350
DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT
360 370 380 390 400
ATMASCDHGV VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG
410 420 430 440 450
RPNENTPKEW LTGYVDYNAK KPAAQEVSPT NGSSEPSKRK LSTSSVEETA
460 470 480 490 500
AAAVSEETPS KDKKKKKKKH KGDEEAPEAA EEEAEPVEKE KKKKKKKDKD

RDRDEAQE
Length:508
Mass (Da):56,831
Last modified:June 1, 1998 - v1
Checksum:i3CAE3F91C84E0A94
GO
Isoform C (identifier: O44081-2) [UniParc]FASTAAdd to basket
Also known as: alpha

The sequence of this isoform differs from the canonical sequence as follows:
     212-253: GVFWVSCEAG...LRRVRSGIQS → DLLIRSCNIW...LKSSHDTLLR
     254-508: Missing.

Show »
Length:253
Mass (Da):28,825
Checksum:i1CA160C68C4D6185
GO

Sequence cautioni

The sequence ABV82369 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69P → R in AAD19897 (PubMed:10087258).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036583212 – 253GVFWV…SGIQS → DLLIRSCNIWTITLIKEISS KIFLKIIECHRNLKSSHDTL LR in isoform C. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_036584254 – 508Missing in isoform C. 1 PublicationAdd BLAST255

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017230 mRNA. Translation: AAC97117.1.
AF089837 mRNA. Translation: AAD16092.1.
AF097634 Genomic DNA. Translation: AAD19897.1.
DQ841550 mRNA. Translation: ABH08965.1.
DQ857345 mRNA. Translation: ABI17547.1.
DQ857346 mRNA. Translation: ABI17548.1.
AE013599 Genomic DNA. Translation: AAF47178.1.
AE013599 Genomic DNA. Translation: AAX52682.1.
AY089408 mRNA. Translation: AAL90146.1.
BT030987 mRNA. Translation: ABV82369.1. Different initiation.
RefSeqiNP_001014547.1. NM_001014547.4. [O44081-1]
NP_001163286.1. NM_001169815.3. [O44081-1]
NP_001163289.1. NM_001169818.3. [O44081-1]
NP_525120.1. NM_080381.5. [O44081-1]
UniGeneiDm.1944.

Genome annotation databases

EnsemblMetazoaiFBtr0072258; FBpp0072166; FBgn0259937. [O44081-1]
FBtr0100213; FBpp0099583; FBgn0259937. [O44081-1]
FBtr0300227; FBpp0289464; FBgn0259937. [O44081-1]
FBtr0300861; FBpp0290083; FBgn0259937. [O44081-1]
GeneIDi37873.
KEGGidme:Dmel_CG3333.
UCSCiCG3333-RB. d. melanogaster.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017230 mRNA. Translation: AAC97117.1.
AF089837 mRNA. Translation: AAD16092.1.
AF097634 Genomic DNA. Translation: AAD19897.1.
DQ841550 mRNA. Translation: ABH08965.1.
DQ857345 mRNA. Translation: ABI17547.1.
DQ857346 mRNA. Translation: ABI17548.1.
AE013599 Genomic DNA. Translation: AAF47178.1.
AE013599 Genomic DNA. Translation: AAX52682.1.
AY089408 mRNA. Translation: AAL90146.1.
BT030987 mRNA. Translation: ABV82369.1. Different initiation.
RefSeqiNP_001014547.1. NM_001014547.4. [O44081-1]
NP_001163286.1. NM_001169815.3. [O44081-1]
NP_001163289.1. NM_001169818.3. [O44081-1]
NP_525120.1. NM_080381.5. [O44081-1]
UniGeneiDm.1944.

3D structure databases

ProteinModelPortaliO44081.
SMRiO44081.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi63449. 4 interactors.
DIPiDIP-20128N.
IntActiO44081. 3 interactors.
MINTiMINT-1030866.
STRINGi7227.FBpp0099583.

PTM databases

iPTMnetiO44081.

Proteomic databases

PaxDbiO44081.
PRIDEiO44081.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0072258; FBpp0072166; FBgn0259937. [O44081-1]
FBtr0100213; FBpp0099583; FBgn0259937. [O44081-1]
FBtr0300227; FBpp0289464; FBgn0259937. [O44081-1]
FBtr0300861; FBpp0290083; FBgn0259937. [O44081-1]
GeneIDi37873.
KEGGidme:Dmel_CG3333.
UCSCiCG3333-RB. d. melanogaster.

Organism-specific databases

CTDi37873.
FlyBaseiFBgn0259937. Nop60B.

Phylogenomic databases

eggNOGiKOG2529. Eukaryota.
COG0130. LUCA.
GeneTreeiENSGT00510000047092.
InParanoidiO44081.
KOiK11131.
OMAiYVDYNAK.
OrthoDBiEOG091G079R.
PhylomeDBiO44081.

Miscellaneous databases

ChiTaRSiNop60B. fly.
GenomeRNAii37873.
PROiO44081.

Gene expression databases

BgeeiFBgn0259937.
ExpressionAtlasiO44081. baseline.
GenevisibleiO44081. DM.

Family and domain databases

Gene3Di2.30.130.10. 1 hit.
InterProiIPR012960. Dyskerin-like.
IPR020103. PsdUridine_synth_cat_dom.
IPR002501. PsdUridine_synth_N.
IPR002478. PUA.
IPR015947. PUA-like_domain.
IPR004802. tRNA_PsdUridine_synth_B_fam.
IPR032819. TruB_C.
[Graphical view]
PANTHERiPTHR23127. PTHR23127. 1 hit.
PfamiPF08068. DKCLD. 1 hit.
PF01472. PUA. 1 hit.
PF16198. TruB_C_2. 1 hit.
PF01509. TruB_N. 1 hit.
[Graphical view]
SMARTiSM01136. DKCLD. 1 hit.
SM00359. PUA. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00425. CBF5. 1 hit.
PROSITEiPS50890. PUA. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDKC1_DROME
AccessioniPrimary (citable) accession number: O44081
Secondary accession number(s): A8E745
, Q0GLB4, Q0MVP1, Q8T408, Q9UAN8, Q9V3Z5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.