Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

H/ACA ribonucleoprotein complex subunit 4

Gene

Nop60B

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a central role in ribosomal RNA processing. Probable catalytic subunit of H/ACA small nucleolar ribonucleoprotein (H/ACA snoRNP) complex, which catalyzes pseudouridylation of rRNA. This involves the isomerization of uridine such that the ribose is subsequently attached to C5, instead of the normal N1. Pseudouridine ('psi') residues may serve to stabilize the conformation of rRNAs. Required for maintenance of the germline stem cell lineage during spermatogenesis.3 Publications

Catalytic activityi

RNA uridine = RNA pseudouridine.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei123NucleophileBy similarity1

GO - Molecular functioni

GO - Biological processi

  • box H/ACA snoRNA 3'-end processing Source: GO_Central
  • germ cell development Source: FlyBase
  • mRNA pseudouridine synthesis Source: GO_Central
  • pseudouridine synthesis Source: FlyBase
  • ribosome biogenesis Source: FlyBase
  • rRNA processing Source: FlyBase
  • rRNA pseudouridine synthesis Source: GO_Central
  • snRNA pseudouridine synthesis Source: GO_Central
  • wing disc development Source: FlyBase

Keywordsi

Molecular functionIsomerase, Ribonucleoprotein, RNA-binding
Biological processRibosome biogenesis, rRNA processing

Names & Taxonomyi

Protein namesi
Recommended name:
H/ACA ribonucleoprotein complex subunit 4 (EC:5.4.99.-)
Alternative name(s):
Nucleolar protein AT band 60B
Protein minifly
Gene namesi
Name:Nop60B
Synonyms:mfl
ORF Names:CG3333
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2R

Organism-specific databases

FlyBaseiFBgn0259937 Nop60B

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001219891 – 508H/ACA ribonucleoprotein complex subunit 4Add BLAST508

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei442Phosphoserine1 Publication1
Modified residuei443Phosphothreonine1 Publication1
Modified residuei444Phosphoserine1 Publication1
Modified residuei445Phosphoserine1 Publication1
Modified residuei449Phosphothreonine1 Publication1
Modified residuei455Phosphoserine1 Publication1
Modified residuei458Phosphothreonine1 Publication1

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO44081
PRIDEiO44081

PTM databases

iPTMnetiO44081

Expressioni

Developmental stagei

Isoform C is expressed both maternally and zygotically. Isoform A is expressed at the same level in adult females but at a much lower level in ovaries.1 Publication

Gene expression databases

BgeeiFBgn0259937
ExpressionAtlasiO44081 baseline and differential
GenevisibleiO44081 DM

Interactioni

Subunit structurei

Component of the small nucleolar ribonucleoprotein particle containing H/ACA-type snoRNAs (H/ACA snoRNPs).By similarity

Protein-protein interaction databases

BioGridi63449, 10 interactors
DIPiDIP-20128N
IntActiO44081, 3 interactors
STRINGi7227.FBpp0099583

Structurei

3D structure databases

ProteinModelPortaliO44081
SMRiO44081
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini294 – 369PUAPROSITE-ProRule annotationAdd BLAST76

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 16Poly-Lys9
Compositional biasi461 – 471Poly-LysAdd BLAST11
Compositional biasi489 – 499Poly-LysAdd BLAST11

Sequence similaritiesi

Belongs to the pseudouridine synthase TruB family.Curated

Phylogenomic databases

eggNOGiKOG2529 Eukaryota
COG0130 LUCA
GeneTreeiENSGT00510000047092
InParanoidiO44081
KOiK11131
OMAiIYQRPPL
OrthoDBiEOG091G079R
PhylomeDBiO44081

Family and domain databases

InterProiView protein in InterPro
IPR012960 Dyskerin-like
IPR020103 PsdUridine_synth_cat_dom_sf
IPR002501 PsdUridine_synth_N
IPR002478 PUA
IPR015947 PUA-like_sf
IPR004802 tRNA_PsdUridine_synth_B_fam
IPR032819 TruB_C
PANTHERiPTHR23127 PTHR23127, 1 hit
PfamiView protein in Pfam
PF08068 DKCLD, 1 hit
PF01472 PUA, 1 hit
PF16198 TruB_C_2, 1 hit
PF01509 TruB_N, 1 hit
SMARTiView protein in SMART
SM01136 DKCLD, 1 hit
SM00359 PUA, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
SSF88697 SSF88697, 1 hit
TIGRFAMsiTIGR00425 CBF5, 1 hit
PROSITEiView protein in PROSITE
PS50890 PUA, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: O44081-1) [UniParc]FASTAAdd to basket
Also known as: B

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADVEVRKEK KKKKIKEEPL DGDDIGTLQK QGNFQIKPSS KIAELDTSQW
60 70 80 90 100
PLLLKNFDKL NIRSNHYTPL AHGSSPLNRD IKEYMKTGFI NLDKPSNPSS
110 120 130 140 150
HEVVAWIKKI LKVEKTGHSG TLDPKVTGCL IVCIDRATRL VKSQQSAGKE
160 170 180 190 200
YVAIFKLHGA VESVAKVRQG LEKLRGALFQ RPPLISAVKR QLRVRTVYDS
210 220 230 240 250
KLLDYDETRN MGVFWVSCEA GSYIRTMCVH LGLVLGVGGQ MLELRRVRSG
260 270 280 290 300
IQSERDGMVT MHDVLDAMWL YENHKDESML RRVIKPLEGL LVNHKRIIMK
310 320 330 340 350
DSSVNAVCYG AKITLPGVLR YEDGIEIDQE IVICTTKGEA ICLAIALMTT
360 370 380 390 400
ATMASCDHGV VAKIKRVIME RDTYPRKWGL GPKASAKKAL IAAGKLDKFG
410 420 430 440 450
RPNENTPKEW LTGYVDYNAK KPAAQEVSPT NGSSEPSKRK LSTSSVEETA
460 470 480 490 500
AAAVSEETPS KDKKKKKKKH KGDEEAPEAA EEEAEPVEKE KKKKKKKDKD

RDRDEAQE
Length:508
Mass (Da):56,831
Last modified:June 1, 1998 - v1
Checksum:i3CAE3F91C84E0A94
GO
Isoform C (identifier: O44081-2) [UniParc]FASTAAdd to basket
Also known as: alpha

The sequence of this isoform differs from the canonical sequence as follows:
     212-253: GVFWVSCEAG...LRRVRSGIQS → DLLIRSCNIW...LKSSHDTLLR
     254-508: Missing.

Show »
Length:253
Mass (Da):28,825
Checksum:i1CA160C68C4D6185
GO

Sequence cautioni

The sequence ABV82369 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69P → R in AAD19897 (PubMed:10087258).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_036583212 – 253GVFWV…SGIQS → DLLIRSCNIWTITLIKEISS KIFLKIIECHRNLKSSHDTL LR in isoform C. 1 PublicationAdd BLAST42
Alternative sequenceiVSP_036584254 – 508Missing in isoform C. 1 PublicationAdd BLAST255

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF017230 mRNA Translation: AAC97117.1
AF089837 mRNA Translation: AAD16092.1
AF097634 Genomic DNA Translation: AAD19897.1
DQ841550 mRNA Translation: ABH08965.1
DQ857345 mRNA Translation: ABI17547.1
DQ857346 mRNA Translation: ABI17548.1
AE013599 Genomic DNA Translation: AAF47178.1
AE013599 Genomic DNA Translation: AAX52682.1
AY089408 mRNA Translation: AAL90146.1
BT030987 mRNA Translation: ABV82369.1 Different initiation.
RefSeqiNP_001014547.1, NM_001014547.4 [O44081-1]
NP_001163286.1, NM_001169815.3 [O44081-1]
NP_001163289.1, NM_001169818.3 [O44081-1]
NP_525120.1, NM_080381.5 [O44081-1]
UniGeneiDm.1944

Genome annotation databases

EnsemblMetazoaiFBtr0072258; FBpp0072166; FBgn0259937 [O44081-1]
FBtr0100213; FBpp0099583; FBgn0259937 [O44081-1]
FBtr0300227; FBpp0289464; FBgn0259937 [O44081-1]
FBtr0300861; FBpp0290083; FBgn0259937 [O44081-1]
GeneIDi37873
KEGGidme:Dmel_CG3333
UCSCiCG3333-RB d. melanogaster

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiDKC1_DROME
AccessioniPrimary (citable) accession number: O44081
Secondary accession number(s): A8E745
, Q0GLB4, Q0MVP1, Q8T408, Q9UAN8, Q9V3Z5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: June 1, 1998
Last modified: May 23, 2018
This is version 149 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health