Unreviewed, UniProtKB/TrEMBL O44049 (O44049_TRYRA)

Names and origin

Protein names	Submitted name:     Sialidase EMBL AAC95493.1     EC=3.2.1.18
Organism	Trypanosoma rangeli EMBL AAC95493.1
Taxonomic identifier	5698 [NCBI]
Taxonomic lineage	Eukaryota › Euglenozoa › Kinetoplastida › Trypanosomatidae › Trypanosoma

Protein attributes

Sequence length	660 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

Ontologies

Keywords
Domain	Signal
Molecular function	Glycosidase Hydrolase
Gene Ontology (GO)
Biological process	metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pathogenesis Inferred from electronic annotation. Source: InterPro
Molecular function	exo-alpha-sialidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential EMBL AAC95493.1
	Chain	23 – 660	638	sialidase EMBL AAC95493.1		PRO_5000145206

Sequences

	Sequence		Length	Mass (Da)
	O44049-1 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: B5D55765DC4423D0	FASTA	660	71,965
	10 20 30 40 50 60 MSWLAVFVPL FLMACASEPA SALAPGSSRV ELFKRKNSTV PFEESNGTIR ERVVHSFRIP 70 80 90 100 110 120 TIVNVDGVMV ATADARYETS FDNSFIETAV KYSVDDGATW NTQIAIKNSR ASSVSRVMDA 130 140 150 160 170 180 TVIVKGNKLY ILVGSFNKTR NSWTQHRDGS DWEPLLVVGE VTKSAANGKT TATISWGKPV 190 200 210 220 230 240 SLKPLFPAEF DGILTKEFIG GVGAAIVGSN GNLVYPVQIA DMGGRVFTKI MYSEDDGNTW 250 260 270 280 290 300 KFAEGRSKFG CSEPAVLEWE GKLIINNRVD GNRRLVYESS DMGKTWVEAL GTLSHVWTNS 310 320 330 340 350 360 PTSNQQDCQS SFVAVTIEGK RVMLFTHPLN LKGRWMRDRL HLWMTDNQRI FDVGQISIGD 370 380 390 400 410 420 ENSGYSSVLY KDDKLYSLHE INTNDVYSLV FVRFIGELQL MKSVVRTWKE EDNHLASICT 430 440 450 460 470 480 PVVPATPPSK GGCGAAVPTA GLVGFLSHSA NGSVWEDVYR CVDANVANAE RVPNGLKFNG 490 500 510 520 530 540 VGGGAVWPVA RQGQTRRYQF ANYRFTLVAT VTIDELPKGT SPLLGAGLEG PGDAKLLGLS 550 560 570 580 590 600 YDKNRQWRPL YGAAPASPTG SWELHKKYHV VLTMADRQGS VYVDGQPLAG SGNTVVRGAT 610 620 630 640 650 660 LPDISHFYIG GPRSKGAPTD SRVTVTNIVL YNRRLNSSEI RTLFLSQDMI GTDGGAGTAA

References

[1]	"Trypanosoma rangeli sialidase: cloning, expression and similarity to T. cruzi trans-sialidase." Buschiazzo A., Campetella O., Frasch A.C. Glycobiology 7:1167-1173(1997) [PubMed: 9455917] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: LDG EMBL AAC95493.1.
[2]	"Structural basis of sialyltransferase activity in trypanosomal sialidases." Buschiazzo A., Tavares G.A., Campetella O., Spinelli S., Cremona M.L., Paris G., Amaya M.F., Frasch A.C., Alzari P.M. EMBO J. 19:16-24(2000) [PubMed: 10619840] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.2 ANGSTROMS) OF 23-660.
[3]	"The high resolution structures of free and inhibitor-bound Trypanosoma rangeli sialidase and its comparison with T. cruzi trans-sialidase." Amaya M.F., Buschiazzo A., Nguyen T., Alzari P.M. J. Mol. Biol. 325:773-784(2003) [PubMed: 12507479] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.6 ANGSTROMS) OF 23-660.
[4]	"A sialidase mutant displaying trans-sialidase activity." Paris G., Ratier L., Amaya M.F., Nguyen T., Alzari P.M., Frasch A.C. J. Mol. Biol. 345:923-934(2005) [PubMed: 15588836] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.8 ANGSTROMS) OF 23-660.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U83180 Genomic DNA. Translation: AAC95493.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1MZ5	X-ray	2.20	A	23-660	[»]
1MZ6	X-ray	2.90	A	23-660	[»]
1N1S	X-ray	1.64	A	23-660	[»]
1N1T	X-ray	1.60	A	23-660	[»]
1N1V	X-ray	2.10	A	23-660	[»]
1N1Y	X-ray	2.80	A	23-660	[»]
1WCS	X-ray	2.80	A	23-660	[»]
2A75	X-ray	1.95	A	23-660	[»]
2AGS	X-ray	1.70	A	23-660	[»]
2FHR	X-ray	2.20	A	23-660	[»]

ModBase

Search...

Protein family/group databases

CAZy

GH33. Glycoside Hydrolase Family 33.

Family and domain databases

InterPro

IPR013320. ConA-like_subgrp.
IPR008377. Sialidase_trypan.
[Graphical view]

Gene3D

G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.

PRINTS

PR01803. TCSIALIDASE.

ProtoNet

Search...

Entry information

Entry name

O44049_TRYRA

Accession

Primary (citable) accession number: O44049

Entry history

Integrated into UniProtKB/TrEMBL:	June 1, 1998
Last sequence update:	June 1, 1998
Last modified:	June 16, 2009
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)