O44006 (KPYK_EIMTE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pyruvate kinase
Short name=PK
EC=2.7.1.40

Gene names

Name:

PYK

Organism

Eimeria tenella (Coccidian parasite)

Taxonomic identifier

5802 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Alveolata › Apicomplexa › Conoidasida › Coccidia › Eucoccidiorida › Eimeriorina › Eimeriidae › Eimeria

Protein attributes

Sequence length	531 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + pyruvate = ADP + phosphoenolpyruvate.
Cofactor	Magnesium. Potassium.
Pathway	Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the pyruvate kinase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium Pyruvate
Molecular function	Kinase Transferase
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro potassium ion binding Inferred from electronic annotation. Source: InterPro pyruvate kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 531	531	Pyruvate kinase		PRO_0000112100
Sites
	Metal binding	88	1	Potassium By similarity
	Metal binding	90	1	Potassium By similarity
	Metal binding	121	1	Potassium By similarity
	Metal binding	122	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	277	1	Magnesium By similarity
	Metal binding	301	1	Magnesium By similarity
	Binding site	86	1	Substrate By similarity
	Binding site	300	1	Substrate; via amide nitrogen By similarity
	Binding site	301	1	Substrate; via amide nitrogen By similarity
	Binding site	333	1	Substrate By similarity
	Site	275	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	O44006 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: C02B2399B863EE08	FASTA	531	57,591
	10 20 30 40 50 60 MANPTHSQVV AAPDSSGRLL RLVSASSVMM QLLGKSTNIR MSQILEHHED EDFSAHRTKI 70 80 90 100 110 120 VCTMGPSCWD VDKMVQLIDA GMNVCRLNFS HGDHEAHGRV VKNLQEALKQ RPGKRVALLL 130 140 150 160 170 180 DTKGPEIRTG MLEGDKPIEL HAGDMLKIVT DYSFVGNKSC IACSYEKLPS SVKPGNTILI 190 200 210 220 230 240 ADGSLSVEVV ECGKDYVMTR VMNPAIIGNK KNMNLPGVKV DLPVIGEKDK NDILNFGIPM 250 260 270 280 290 300 GCNFIAASFV QSADDVRYIR SILGTKGRNI KIIPKIENVE GLLNFDEILQ EADGIMIARG 310 320 330 340 350 360 DLGMEIPPEK VFLAQKMMIS KCNVAGKPVI TATQMLESMT KNPRPTRAEA ADVANAVLDG 370 380 390 400 410 420 TDCVMLSGET ANGSFPVQAV TVMSRVCFEA EGCIDYQQVF RATCQATMTP IDTQEAVARA 430 440 450 460 470 480 AVETAQSINA SLILALTETG RTARLIAKYR PMQPILALSA SEETIKQLQV IRGVTTFLVP 490 500 510 520 530 TFQGTDQLIR NALSAAKELQ LVSEGDSIVA VHGIKEEVAG WSNLLKVLVV E

References

[1]	"Molecular cloning of the gene for pyruvate kinase of Eimeria tenella." Labbe M., Ouarzane M., Bourdieu C., Pery P. Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: PAPt38.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF043910 mRNA. Translation: AAC02529.1.
3D structure databases
ProteinModelPortal	O44006.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	2.7.1.40. 2046.
UniPathway	UPA00109; UER00188.
Family and domain databases
Gene3D	2.40.33.10. 1 hit. 3.20.20.60. 2 hits. 3.40.1380.20. 1 hit.
InterPro	IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase. IPR011037. Pyrv_Knase-like_insert_dom. IPR015794. Pyrv_Knase_a/b. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C. IPR015806. Pyrv_Knase_insert_dom. [Graphical view]
PANTHER	PTHR11817. PTHR11817. 1 hit.
Pfam	PF00224. PK. 1 hit. PF02887. PK_C. 1 hit. [Graphical view]
PRINTS	PR01050. PYRUVTKNASE.
SUPFAM	SSF50800. PK_B_barrel_like. 1 hit. SSF52935. Pyruvate_kinase. 1 hit. SSF51621. Pyrv/PenolPyrv_Kinase_cat. 1 hit.
TIGRFAMs	TIGR01064. pyruv_kin. 1 hit.
PROSITE	PS00110. PYRUVATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPYK_EIMTE

Accession

Primary (citable) accession number: O44006

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	June 1, 1998
Last modified:	March 6, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)