PYK

Functionⁱ

Catalytic activityⁱ

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactorⁱ

Protein has several cofactor binding sites:

Pathwayⁱ: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:

Glyceraldehyde-3-phosphate dehydrogenase (ETH_00008865)
no protein annotated in this organism
no protein annotated in this organism
Enolase (ENO)
Pyruvate kinase (PYK), Pyruvate kinase (ETH_00011145), Pyruvate kinase

This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature key	Position(s)	DescriptionActions	Length
Binding siteⁱ	86	SubstrateBy similarity	1
Metal bindingⁱ	88	PotassiumBy similarity	1
Metal bindingⁱ	90	PotassiumBy similarity	1
Metal bindingⁱ	121	PotassiumBy similarity	1
Metal bindingⁱ	122	Potassium; via carbonyl oxygenBy similarity	1
Siteⁱ	275	Transition state stabilizerBy similarity	1
Metal bindingⁱ	277	MagnesiumBy similarity	1
Binding siteⁱ	300	Substrate; via amide nitrogenBy similarity	1
Metal bindingⁱ	301	MagnesiumBy similarity	1
Binding siteⁱ	301	Substrate; via amide nitrogenBy similarity	1
Binding siteⁱ	333	SubstrateBy similarity	1

GO - Molecular functionⁱ

ATP binding Source: UniProtKB-KW
kinase activity Source: UniProtKB-KW
magnesium ion binding Source: InterPro
potassium ion binding Source: InterPro
pyruvate kinase activity Source: UniProtKB-EC

Complete GO annotation...

Keywordsⁱ

Molecular function	Kinase, Transferase
Biological process	Glycolysis
Ligand	ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

BRENDAⁱ	2.7.1.40. 2046.
UniPathwayⁱ	UPA00109; UER00188.

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Pyruvate kinase (EC:2.7.1.40) Short name: PK
Gene namesⁱ	Name:PYK
Organismⁱ	Eimeria tenella (Coccidian parasite)
Taxonomic identifierⁱ	5802 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Alveolata › Apicomplexa › Conoidasida › Coccidia › Eucoccidiorida › Eimeriorina › Eimeriidae › Eimeria

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000112100	1 – 531	Pyruvate kinaseAdd BLAST		531

Interactionⁱ

Subunit structureⁱ

Homotetramer.By similarity

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	O44006.
SMRⁱ	O44006.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Sequence similaritiesⁱ

Belongs to the pyruvate kinase family.Curated

Family and domain databases

Gene3Dⁱ	3.40.1380.20. 1 hit.
InterProⁱ	View protein in InterPro IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C.
PANTHERⁱ	PTHR11817. PTHR11817. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00224. PK. 1 hit. PF02887. PK_C. 1 hit.
PRINTSⁱ	PR01050. PYRUVTKNASE.
SUPFAMⁱ	SSF50800. SSF50800. 1 hit. SSF51621. SSF51621. 2 hits. SSF52935. SSF52935. 1 hit.
TIGRFAMsⁱ	TIGR01064. pyruv_kin. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00110. PYRUVATE_KINASE. 1 hit.

Sequenceⁱ

Sequence statusⁱ: Complete.

O44006-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MANPTHSQVV AAPDSSGRLL RLVSASSVMM QLLGKSTNIR MSQILEHHED 
        60         70         80         90        100
EDFSAHRTKI VCTMGPSCWD VDKMVQLIDA GMNVCRLNFS HGDHEAHGRV 
       110        120        130        140        150
VKNLQEALKQ RPGKRVALLL DTKGPEIRTG MLEGDKPIEL HAGDMLKIVT 
       160        170        180        190        200
DYSFVGNKSC IACSYEKLPS SVKPGNTILI ADGSLSVEVV ECGKDYVMTR 
       210        220        230        240        250
VMNPAIIGNK KNMNLPGVKV DLPVIGEKDK NDILNFGIPM GCNFIAASFV 
       260        270        280        290        300
QSADDVRYIR SILGTKGRNI KIIPKIENVE GLLNFDEILQ EADGIMIARG 
       310        320        330        340        350
DLGMEIPPEK VFLAQKMMIS KCNVAGKPVI TATQMLESMT KNPRPTRAEA 
       360        370        380        390        400
ADVANAVLDG TDCVMLSGET ANGSFPVQAV TVMSRVCFEA EGCIDYQQVF 
       410        420        430        440        450
RATCQATMTP IDTQEAVARA AVETAQSINA SLILALTETG RTARLIAKYR 
       460        470        480        490        500
PMQPILALSA SEETIKQLQV IRGVTTFLVP TFQGTDQLIR NALSAAKELQ 
       510        520        530 
LVSEGDSIVA VHGIKEEVAG WSNLLKVLVV E

Length:531

Mass (Da):57,591

Last modified:June 1, 1998 - v1

Checksum:ⁱC02B2399B863EE08

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF043910 mRNA. Translation: AAC02529.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_013232798.1. XM_013377344.1.

Genome annotation databases

GeneIDⁱ	25251418.

GeneIDⁱ

25251418.

Similar proteinsⁱ

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:

100%	UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%	UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%	UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF043910 mRNA. Translation: AAC02529.1.
NCBI Reference Sequences More...RefSeqⁱ	XP_013232798.1. XM_013377344.1.

3D structure databases

ProteinModelPortalⁱ	O44006.
SMRⁱ	O44006.
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	25251418.

GeneIDⁱ

25251418.

Enzyme and pathway databases

UniPathwayⁱ	UPA00109; UER00188.
BRENDAⁱ	2.7.1.40. 2046.

Family and domain databases

Gene3Dⁱ	3.40.1380.20. 1 hit.
InterProⁱ	View protein in InterPro IPR001697. Pyr_Knase. IPR015813. Pyrv/PenolPyrv_Kinase-like_dom. IPR011037. Pyrv_Knase-like_insert_dom. IPR018209. Pyrv_Knase_AS. IPR015793. Pyrv_Knase_brl. IPR015795. Pyrv_Knase_C.
PANTHERⁱ	PTHR11817. PTHR11817. 1 hit.
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00224. PK. 1 hit. PF02887. PK_C. 1 hit.
PRINTSⁱ	PR01050. PYRUVTKNASE.
SUPFAMⁱ	SSF50800. SSF50800. 1 hit. SSF51621. SSF51621. 2 hits. SSF52935. SSF52935. 1 hit.
TIGRFAMsⁱ	TIGR01064. pyruv_kin. 1 hit.
PROSITEⁱ	View protein in PROSITE PS00110. PYRUVATE_KINASE. 1 hit.
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	KPYK_EIMTE
Accessionⁱ	O44006Primary (citable) accession number: O44006
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
	Last sequence update:	June 1, 1998
	Last modified:	May 10, 2017
	This is version 80 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)

Miscellaneousⁱ

Documents

PATHWAY comments
Index of metabolic and biosynthesis pathways
SIMILARITY comments
Index of protein domains and families

UniProtKB - O44006 (KPYK_EIMTE)

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Pyruvate kinase

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Functionⁱ

Catalytic activityⁱ

Cofactorⁱ

Pathwayⁱ: glycolysis

Sites

GO - Molecular functionⁱ

Keywordsⁱ

Enzyme and pathway databases

Names & Taxonomyⁱ

PTM / Processingⁱ

Molecule processing

Interactionⁱ

Subunit structureⁱ

Structureⁱ

3D structure databases

Family & Domainsⁱ

Sequence similaritiesⁱ

Family and domain databases

Sequenceⁱ

Sequence databases

Genome annotation databases

Similar proteinsⁱ

Cross-referencesⁱ

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

Entry informationⁱ

Miscellaneousⁱ

Documents

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - O44006 (KPYK_EIMTE)

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Pyruvate kinase

PYK

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">‘Function’</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: glycolysis

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Family and domain databases

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including length and molecular weight.<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Documents

Functionⁱ

Pathwayⁱ: glycolysis

GO - Molecular functionⁱ

Names & Taxonomyⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Sequenceⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ