Reviewed, UniProtKB/Swiss-Prot O43929 (ORC4_HUMAN)

Names and origin

Protein names

Recommended name:
    Origin recognition complex subunit 4

Gene names

Name:	ORC4L
Synonyms:	ORC4

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Component of the origin recognition complex (ORC) that binds origins of replication. Binds to the ARS consensus sequence (ACS) of origins of replication in an ATP-dependent manner.
Subunit structure	ORC is composed of six subunits. Interacts with DBF4 By similarity.
Subcellular location	Nucleus.
Sequence similarities	Belongs to the ORC4 family.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Coding sequence diversity	Polymorphism
Ligand	ATP-binding DNA-binding Nucleotide-binding
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication initiation Ref.1 Inferred from mutant phenotype. Source: UniProtKB
Cellular component	nucleoplasm Inferred from Experiment. Source: Reactome origin recognition complex Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA replication origin binding Ref.1 Inferred from mutant phenotype. Source: UniProtKB nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
CCND1	P24385	1	EBI-374889,EBI-375001
CDKN2A	P42771	1	EBI-374889,EBI-375053
MCM10	Q7L590	1	EBI-374889,EBI-374912
MCM3	P25205	1	EBI-374889,EBI-355153
MCM7	P33993	1	EBI-374889,EBI-355924
ORC2L	Q13416	1	EBI-374889,EBI-374957
ORC3L	Q9UBD5	1	EBI-374889,EBI-374916
ORC5L	O43913	1	EBI-374889,EBI-374928
ORC6L	Q9Y5N6	1	EBI-374889,EBI-374840

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Origin recognition complex subunit 4		PRO_0000127087
Regions
	Nucleotide binding	67 – 74	8	ATP Potential
Natural variations
	Natural variant	56	1	L → V: dbSNP rs2307397. Ref.4		VAR_014523
	Natural variant	78	1	N → S: dbSNP rs2307394. Ref.1 Ref.2 Ref.3		VAR_019235

Sequences

	Sequence		Length	Mass (Da)
	O43929-1 [UniParc]. Last modified June 7, 2005. Version 2. Checksum: A7020B6690E30B4E	FASTA	436	50,377
	10 20 30 40 50 60 MSSRKSKSNS LIHTECLSQV QRILRERFCR QSPHSNLFGV QVQYKHLSEL LKRTALHGES 70 80 90 100 110 120 NSVLIIGPRG SGKTMLINHA LKELMEIEEV SENVLQVHLN GLLQINDKIA LKEITRQLNL 130 140 150 160 170 180 ENVVGDKVFG SFAENLSFLL EALKKGDRTS SCPVIFILDE FDLFAHHKNQ TLLYNLFDIS 190 200 210 220 230 240 QSAQTPIAVI GLTCRLDILE LLEKRVKSRF SHRQIHLMNS FGFPQYVKIF KEQLSLPAEF 250 260 270 280 290 300 PDKVFAEKWN ENVQYLSEDR SVQEVLQKHF NISKNLRSLH MLLMLALNRV TASHPFMTAV 310 320 330 340 350 360 DLMEASQLCS MDSKANIVHG LSVLEICLII AMKHLNDIYE EEPFNFQMVY NEFQKFVQRK 370 380 390 400 410 420 AHSVYNFEKP VVMKAFEHLQ QLELIKPMER TSGNSQREYQ LMKLLLDNTQ IMNALQKYPN 430 CPTDVRQWAT SSLSWL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of HsORC4, a member of the human origin of replication recognition complex." Quintana D.G., Hou Z.H., Thome K.C., Hendricks M., Saha P., Dutta A. J. Biol. Chem. 272:28247-28251(1997) [PubMed: 9353276] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-78.
[2]	"The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p." Komrskova T., Yang H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B. Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-78.
[3]	"cDNA cloning of a homolog for S. cerevisiae ORC4 from H. sapiens." Dean F.B., O'Donnell M. Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT SER-78.
[4]	NIEHS SNPs program Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], VARIANT VAL-56.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Placenta.
[6]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

NIEHS-SNPs

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022108 mRNA. Translation: AAC01957.1. AF047598 mRNA. Translation: AAC80282.1. AF132596 mRNA. Translation: AAD22110.1. AY600302 Genomic DNA. Translation: AAS94326.1. BC014847 mRNA. Translation: AAH14847.1.
IPI	IPI00015164.
RefSeq	NP_002543.2. NP_859525.1. NP_859526.1.
UniGene	Hs.558364
3D structure databases
SMR	O43929. Positions 35-419.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-29690N.
IntAct	O43929. 9 interactions.
STRING	O43929.
Proteomic databases
PeptideAtlas	O43929.
PRIDE	O43929.
Genome annotation databases
Ensembl	ENST00000264169; ENSP00000264169; ENSG00000115947; Homo sapiens. [Genome view] ENST00000392857; ENSP00000376597; ENSG00000115947; Homo sapiens. [Genome view] ENST00000392858; ENSP00000376598; ENSG00000115947; Homo sapiens. [Genome view]
GeneID	5000.
KEGG	hsa:5000.
UCSC	uc002twi.1. human.
Organism-specific databases
CTD	5000.
GeneCards	GC02M148408.
H-InvDB	HIX0002492.
HGNC	HGNC:8490. ORC4L.
HPA	CAB015124.
MIM	603056. gene.
PharmGKB	PA32811.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04148.
HOGENOM	HBG717246.
HOVERGEN	O43929.
InParanoid	O43929.
OMA	HALKELM.
OrthoDB	EOG96HJX5.
PhylomeDB	O43929.
Enzyme and pathway databases
Reactome	REACT_152. Cell Cycle, Mitotic. REACT_1538. Cell Cycle Checkpoints. REACT_383. DNA Replication.
Gene expression databases
ArrayExpress	O43929.
Bgee	O43929.
CleanEx	HS_ORC4L.
Genevestigator	O43929.
GermOnline	ENSG00000115947. Homo sapiens.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR016527. ORC4. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
PIRSF	PIRSF007858. ORC4. 1 hit.
SMART	SM00382. AAA. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	19248.
SOURCE	Search...

Entry information

Entry name

ORC4_HUMAN

Accession

Primary (citable) accession number: O43929
Secondary accession number(s): Q96C42

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	June 7, 2005
Last modified:	February 9, 2010
This is version 86 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.