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Protein

NADH dehydrogenase [ubiquinone] iron-sulfur protein 5

Gene

NDUFS5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.

GO - Molecular functioni

  1. NADH dehydrogenase (ubiquinone) activity Source: UniProtKB

GO - Biological processi

  1. cellular metabolic process Source: Reactome
  2. mitochondrial electron transport, NADH to ubiquinone Source: UniProtKB
  3. mitochondrial respiratory chain complex I assembly Source: UniProtKB
  4. respiratory electron transport chain Source: Reactome
  5. small molecule metabolic process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Electron transport, Respiratory chain, Transport

Enzyme and pathway databases

ReactomeiREACT_22393. Respiratory electron transport.

Names & Taxonomyi

Protein namesi
Recommended name:
NADH dehydrogenase [ubiquinone] iron-sulfur protein 5
Alternative name(s):
Complex I-15 kDa
Short name:
CI-15 kDa
NADH-ubiquinone oxidoreductase 15 kDa subunit
Gene namesi
Name:NDUFS5
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 1

Organism-specific databases

HGNCiHGNC:7712. NDUFS5.

Subcellular locationi

GO - Cellular componenti

  1. mitochondrial inner membrane Source: Reactome
  2. mitochondrial intermembrane space Source: UniProtKB-SubCell
  3. mitochondrial respiratory chain complex I Source: UniProtKB
  4. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA31522.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 106105NADH dehydrogenase [ubiquinone] iron-sulfur protein 5PRO_0000118786Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 66Sequence Analysis
Disulfide bondi43 ↔ 56Sequence Analysis

Keywords - PTMi

Disulfide bond

Proteomic databases

MaxQBiO43920.
PaxDbiO43920.
PeptideAtlasiO43920.
PRIDEiO43920.

PTM databases

PhosphoSiteiO43920.

Expressioni

Gene expression databases

BgeeiO43920.
CleanExiHS_NDUFS5.
ExpressionAtlasiO43920. baseline and differential.
GenevestigatoriO43920.

Organism-specific databases

HPAiHPA042582.

Interactioni

Subunit structurei

Mammalian complex I is composed of 45 different subunits. This is a component of the iron-sulfur (IP) fragment of the enzyme.1 Publication

Protein-protein interaction databases

BioGridi110804. 2 interactions.
IntActiO43920. 4 interactions.
MINTiMINT-2796423.
STRINGi9606.ENSP00000362058.

Structurei

3D structure databases

ProteinModelPortaliO43920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi33 – 4311C-X9-C motif 1Add
BLAST
Motifi56 – 6611C-X9-C motif 2Add
BLAST

Domaini

Contains two C-X9-C motifs that are predicted to form a helix-coil-helix structure, permitting the formation of intramolecular disulfide bonds.1 Publication

Sequence similaritiesi

Belongs to the complex I NDUFS5 subunit family.Curated

Phylogenomic databases

eggNOGiNOG313534.
HOGENOMiHOG000264236.
HOVERGENiHBG001646.
InParanoidiO43920.
KOiK03938.
OMAiLRQKTMK.
OrthoDBiEOG7C8GKB.
PhylomeDBiO43920.
TreeFamiTF332111.

Family and domain databases

InterProiIPR019342. NADH_UbQ_OxRdtase_FeS-su5.
[Graphical view]
PfamiPF10200. Ndufs5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPFLDIQKRF GLNIDRWLTI QSGEQPYKMA GRCHAFEKEW IECAHGIGYT
60 70 80 90 100
RAEKECKIEY DDFVECLLRQ KTMRRAGTIR KQRDKLIKEG KYTPPPHHIG

KGEPRP
Length:106
Mass (Da):12,518
Last modified:January 23, 2007 - v3
Checksum:i3D55402837DD99EE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti96 – 961P → S Detected in a patient with mitochondrial complex I deficiency; uncertain pathological significance. 1 Publication
VAR_064569

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047434 mRNA. Translation: AAC39878.1.
AF020352 mRNA. Translation: AAB87866.1.
BC001884 mRNA. Translation: AAH01884.1.
CCDSiCCDS434.1.
RefSeqiNP_001171908.1. NM_001184979.1.
NP_004543.1. NM_004552.2.
UniGeneiHs.632385.

Genome annotation databases

EnsembliENST00000372967; ENSP00000362058; ENSG00000168653.
ENST00000372969; ENSP00000362060; ENSG00000168653.
GeneIDi4725.
KEGGihsa:4725.
UCSCiuc001ccx.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047434 mRNA. Translation: AAC39878.1.
AF020352 mRNA. Translation: AAB87866.1.
BC001884 mRNA. Translation: AAH01884.1.
CCDSiCCDS434.1.
RefSeqiNP_001171908.1. NM_001184979.1.
NP_004543.1. NM_004552.2.
UniGeneiHs.632385.

3D structure databases

ProteinModelPortaliO43920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110804. 2 interactions.
IntActiO43920. 4 interactions.
MINTiMINT-2796423.
STRINGi9606.ENSP00000362058.

Chemistry

ChEMBLiCHEMBL2363065.

PTM databases

PhosphoSiteiO43920.

Proteomic databases

MaxQBiO43920.
PaxDbiO43920.
PeptideAtlasiO43920.
PRIDEiO43920.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000372967; ENSP00000362058; ENSG00000168653.
ENST00000372969; ENSP00000362060; ENSG00000168653.
GeneIDi4725.
KEGGihsa:4725.
UCSCiuc001ccx.3. human.

Organism-specific databases

CTDi4725.
GeneCardsiGC01P039491.
HGNCiHGNC:7712. NDUFS5.
HPAiHPA042582.
MIMi603847. gene.
neXtProtiNX_O43920.
PharmGKBiPA31522.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG313534.
HOGENOMiHOG000264236.
HOVERGENiHBG001646.
InParanoidiO43920.
KOiK03938.
OMAiLRQKTMK.
OrthoDBiEOG7C8GKB.
PhylomeDBiO43920.
TreeFamiTF332111.

Enzyme and pathway databases

ReactomeiREACT_22393. Respiratory electron transport.

Miscellaneous databases

ChiTaRSiNDUFS5. human.
GeneWikiiNDUFS5.
GenomeRNAii4725.
NextBioi18222.
PROiO43920.
SOURCEiSearch...

Gene expression databases

BgeeiO43920.
CleanExiHS_NDUFS5.
ExpressionAtlasiO43920. baseline and differential.
GenevestigatoriO43920.

Family and domain databases

InterProiIPR019342. NADH_UbQ_OxRdtase_FeS-su5.
[Graphical view]
PfamiPF10200. Ndufs5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of genes expressed in human CD34(+) hematopoietic stem/progenitor cells by expressed sequence tags and efficient full-length cDNA cloning."
    Mao M., Fu G., Wu J.-S., Zhang Q.-H., Zhou J., Kan L.-X., Huang Q.-H., He K.-L., Gu B.-W., Han Z.-G., Shen Y., Gu J., Yu Y.-P., Xu S.-H., Wang Y.-X., Chen S.-J., Chen Z.
    Proc. Natl. Acad. Sci. U.S.A. 95:8175-8180(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Umbilical cord blood.
  2. "The human NADH:ubiquinone oxidoreductase NDUFS5 (15 kDa) subunit: cDNA cloning, chromosomal localization, tissue distribution and the absence of mutations in isolated complex I-deficient patients."
    Loeffen J., Smeets R., Smeitink J., Triepels R., Sengers R., Trijbels F., van den Heuvel L.
    J. Inherit. Metab. Dis. 22:19-28(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.
  4. "The subunit composition of the human NADH dehydrogenase obtained by rapid one-step immunopurification."
    Murray J., Zhang B., Taylor S.W., Oglesbee D., Fahy E., Marusich M.F., Ghosh S.S., Capaldi R.A.
    J. Biol. Chem. 278:13619-13622(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE NADH-UBIQUINONE OXIDOREDUCTASE COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  6. "NDUFB7 and NDUFA8 are located at the intermembrane surface of complex I."
    Szklarczyk R., Wanschers B.F., Nabuurs S.B., Nouws J., Nijtmans L.G., Huynen M.A.
    FEBS Lett. 585:737-743(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN, MOTIF.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  9. Cited for: VARIANT SER-96.

Entry informationi

Entry nameiNDUS5_HUMAN
AccessioniPrimary (citable) accession number: O43920
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 121 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.