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Protein

Carbohydrate sulfotransferase 1

Gene

CHST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.2 Publications

Catalytic activityi

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi69 – 757PAPSBy similarity
Nucleotide bindingi234 – 2429PAPSBy similarity

GO - Molecular functioni

  • keratan sulfotransferase activity Source: UniProtKB
  • sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • carbohydrate metabolic process Source: Reactome
  • galactose metabolic process Source: UniProtKB
  • glycosaminoglycan metabolic process Source: Reactome
  • inflammatory response Source: UniProtKB-KW
  • keratan sulfate biosynthetic process Source: Reactome
  • keratan sulfate metabolic process Source: UniProtKB
  • polysaccharide metabolic process Source: ProtInc
  • small molecule metabolic process Source: Reactome
  • sulfur compound metabolic process Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Carbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

BRENDAi2.8.2.21. 2681.
ReactomeiREACT_121120. Keratan sulfate biosynthesis.
SABIO-RKO43916.

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name:
KS6ST
Short name:
KSGal6ST
Short name:
KSST
Gene namesi
Name:CHST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 11

Organism-specific databases

HGNCiHGNC:1969. CHST1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 22CytoplasmicSequence Analysis
Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST
Topological domaini24 – 411388LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • Golgi membrane Source: Reactome
  • integral component of membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26501.

Polymorphism and mutation databases

BioMutaiCHST1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 411411Carbohydrate sulfotransferase 1PRO_0000085182Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO43916.
PRIDEiO43916.

PTM databases

PhosphoSiteiO43916.

Expressioni

Tissue specificityi

Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.2 Publications

Gene expression databases

BgeeiO43916.
CleanExiHS_CHST1.
GenevisibleiO43916. HS.

Interactioni

Protein-protein interaction databases

BioGridi114104. 2 interactions.
IntActiO43916. 2 interactions.
MINTiMINT-1440600.
STRINGi9606.ENSP00000309270.

Structurei

3D structure databases

ProteinModelPortaliO43916.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi337 – 3393Cell attachment siteSequence Analysis

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO43916.
KOiK01022.
OMAiCEEGPTF.
OrthoDBiEOG7RZ5S0.
PhylomeDBiO43916.
TreeFamiTF342871.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

O43916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLAEA GLAERLCEES
60 70 80 90 100
PTFAYNLSRK THILILATTR SGSSFVGQLF NQHLDVFYLF EPLYHVQNTL
110 120 130 140 150
IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR
160 170 180 190 200
IFRRGASRVL CSRPVCDPPG PADLVLEEGD CVRKCGLLNL TVAAEACRER
210 220 230 240 250
SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT
260 270 280 290 300
YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV
310 320 330 340 350
RYEDLARNPM KKTEEIYGFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV
360 370 380 390 400
RNSAATAEKW RFRLSYDIVA FAQNACQQVL AQLGYKIAAS EEELKNPSVS
410
LVEERDFRPF S
Length:411
Mass (Da):46,715
Last modified:June 1, 1998 - v1
Checksum:iF07D0A23B6338A09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003791 mRNA. Translation: BAA24840.1.
U65637 mRNA. Translation: AAC28776.1.
AF090137 mRNA. Translation: AAD19878.1.
AY339617 Genomic DNA. Translation: AAP88041.1.
CH471064 Genomic DNA. Translation: EAW68035.1.
CH471064 Genomic DNA. Translation: EAW68038.1.
BC022567 mRNA. Translation: AAH22567.1.
BC028235 mRNA. Translation: AAH28235.1.
CCDSiCCDS7913.1.
RefSeqiNP_003645.1. NM_003654.5.
UniGeneiHs.104576.

Genome annotation databases

EnsembliENST00000308064; ENSP00000309270; ENSG00000175264.
GeneIDi8534.
KEGGihsa:8534.
UCSCiuc001mys.2. human.

Cross-referencesi

Web resourcesi

GGDB

GlycoGene database

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003791 mRNA. Translation: BAA24840.1.
U65637 mRNA. Translation: AAC28776.1.
AF090137 mRNA. Translation: AAD19878.1.
AY339617 Genomic DNA. Translation: AAP88041.1.
CH471064 Genomic DNA. Translation: EAW68035.1.
CH471064 Genomic DNA. Translation: EAW68038.1.
BC022567 mRNA. Translation: AAH22567.1.
BC028235 mRNA. Translation: AAH28235.1.
CCDSiCCDS7913.1.
RefSeqiNP_003645.1. NM_003654.5.
UniGeneiHs.104576.

3D structure databases

ProteinModelPortaliO43916.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114104. 2 interactions.
IntActiO43916. 2 interactions.
MINTiMINT-1440600.
STRINGi9606.ENSP00000309270.

PTM databases

PhosphoSiteiO43916.

Polymorphism and mutation databases

BioMutaiCHST1.

Proteomic databases

PaxDbiO43916.
PRIDEiO43916.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000308064; ENSP00000309270; ENSG00000175264.
GeneIDi8534.
KEGGihsa:8534.
UCSCiuc001mys.2. human.

Organism-specific databases

CTDi8534.
GeneCardsiGC11M045670.
HGNCiHGNC:1969. CHST1.
MIMi603797. gene.
neXtProtiNX_O43916.
PharmGKBiPA26501.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG80862.
GeneTreeiENSGT00530000062902.
HOGENOMiHOG000261614.
HOVERGENiHBG106811.
InParanoidiO43916.
KOiK01022.
OMAiCEEGPTF.
OrthoDBiEOG7RZ5S0.
PhylomeDBiO43916.
TreeFamiTF342871.

Enzyme and pathway databases

BRENDAi2.8.2.21. 2681.
ReactomeiREACT_121120. Keratan sulfate biosynthesis.
SABIO-RKO43916.

Miscellaneous databases

GeneWikiiCHST1.
GenomeRNAii8534.
NextBioi31964.
PROiO43916.
SOURCEiSearch...

Gene expression databases

BgeeiO43916.
CleanExiHS_CHST1.
GenevisibleiO43916. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamiPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMiSSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase."
    Fukuta M., Inazawa J., Torii T., Tsuzuki K., Shimada E., Habuchi O.
    J. Biol. Chem. 272:32321-32328(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
    Tissue: Fetal brain.
  2. "Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization."
    Mazany K.D., Peng T., Watson C.E., Tabas I., Williams K.J.
    Biochim. Biophys. Acta 1407:92-97(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization."
    Li X., Tedder T.F.
    Genomics 55:345-347(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Umbilical vein endothelial cell.
  4. NIEHS SNPs program
    Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  7. "Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase."
    Torii T., Fukuta M., Habuchi O.
    Glycobiology 10:203-211(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBSTRATE SPECIFICITY, FUNCTION.
  8. "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin."
    Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S.
    J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. "CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin."
    Li X., Tu L., Murphy P.G., Kadono T., Steeber D.A., Tedder T.F.
    J. Leukoc. Biol. 69:565-574(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiCHST1_HUMAN
AccessioniPrimary (citable) accession number: O43916
Secondary accession number(s): D3DQP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: June 24, 2015
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Caution

Was originally (PubMed:9639683) thought to be the ortholog of chicken CHST3 and therefore named C6ST. However, it has no strong chondroitin 6-sulfotransferase activity.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 11
    Human chromosome 11: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.