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O43916

- CHST1_HUMAN

UniProt

O43916 - CHST1_HUMAN

Protein

Carbohydrate sulfotransferase 1

Gene

CHST1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 111 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.2 Publications

    Catalytic activityi

    3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi69 – 757PAPSBy similarity
    Nucleotide bindingi234 – 2429PAPSBy similarity

    GO - Molecular functioni

    1. keratan sulfotransferase activity Source: UniProtKB
    2. sulfotransferase activity Source: UniProtKB

    GO - Biological processi

    1. carbohydrate metabolic process Source: Reactome
    2. galactose metabolic process Source: UniProtKB
    3. glycosaminoglycan metabolic process Source: Reactome
    4. inflammatory response Source: UniProtKB-KW
    5. keratan sulfate biosynthetic process Source: Reactome
    6. keratan sulfate metabolic process Source: UniProtKB
    7. polysaccharide metabolic process Source: ProtInc
    8. small molecule metabolic process Source: Reactome
    9. sulfur compound metabolic process Source: UniProtKB

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Inflammatory response

    Enzyme and pathway databases

    BRENDAi2.8.2.21. 2681.
    ReactomeiREACT_121120. Keratan sulfate biosynthesis.
    SABIO-RKO43916.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
    Alternative name(s):
    Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
    Short name:
    GST-1
    Keratan sulfate Gal-6 sulfotransferase
    Short name:
    KS6ST
    Short name:
    KSGal6ST
    Short name:
    KSST
    Gene namesi
    Name:CHST1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1969. CHST1.

    Subcellular locationi

    GO - Cellular componenti

    1. Golgi membrane Source: Reactome
    2. integral component of membrane Source: UniProtKB

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26501.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 411411Carbohydrate sulfotransferase 1PRO_0000085182Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi56 – 561N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi145 – 1451N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi189 – 1891N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi334 – 3341N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO43916.
    PRIDEiO43916.

    PTM databases

    PhosphoSiteiO43916.

    Expressioni

    Tissue specificityi

    Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.2 Publications

    Gene expression databases

    BgeeiO43916.
    CleanExiHS_CHST1.
    GenevestigatoriO43916.

    Interactioni

    Protein-protein interaction databases

    BioGridi114104. 2 interactions.
    IntActiO43916. 2 interactions.
    MINTiMINT-1440600.
    STRINGi9606.ENSP00000309270.

    Structurei

    3D structure databases

    ProteinModelPortaliO43916.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 22CytoplasmicSequence Analysis
    Topological domaini24 – 411388LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei3 – 2321Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi337 – 3393Cell attachment siteSequence Analysis

    Sequence similaritiesi

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG80862.
    HOGENOMiHOG000261614.
    HOVERGENiHBG106811.
    InParanoidiO43916.
    KOiK01022.
    OMAiCEEGPTF.
    OrthoDBiEOG7RZ5S0.
    PhylomeDBiO43916.
    TreeFamiTF342871.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view]
    PfamiPF00685. Sulfotransfer_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMiSSF52540. SSF52540. 2 hits.

    Sequencei

    Sequence statusi: Complete.

    O43916-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLAEA GLAERLCEES    50
    PTFAYNLSRK THILILATTR SGSSFVGQLF NQHLDVFYLF EPLYHVQNTL 100
    IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR 150
    IFRRGASRVL CSRPVCDPPG PADLVLEEGD CVRKCGLLNL TVAAEACRER 200
    SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT 250
    YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV 300
    RYEDLARNPM KKTEEIYGFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV 350
    RNSAATAEKW RFRLSYDIVA FAQNACQQVL AQLGYKIAAS EEELKNPSVS 400
    LVEERDFRPF S 411
    Length:411
    Mass (Da):46,715
    Last modified:June 1, 1998 - v1
    Checksum:iF07D0A23B6338A09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003791 mRNA. Translation: BAA24840.1.
    U65637 mRNA. Translation: AAC28776.1.
    AF090137 mRNA. Translation: AAD19878.1.
    AY339617 Genomic DNA. Translation: AAP88041.1.
    CH471064 Genomic DNA. Translation: EAW68035.1.
    CH471064 Genomic DNA. Translation: EAW68038.1.
    BC022567 mRNA. Translation: AAH22567.1.
    BC028235 mRNA. Translation: AAH28235.1.
    CCDSiCCDS7913.1.
    RefSeqiNP_003645.1. NM_003654.5.
    UniGeneiHs.104576.

    Genome annotation databases

    EnsembliENST00000308064; ENSP00000309270; ENSG00000175264.
    GeneIDi8534.
    KEGGihsa:8534.
    UCSCiuc001mys.2. human.

    Cross-referencesi

    Web resourcesi

    GGDB

    GlycoGene database

    NIEHS-SNPs

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AB003791 mRNA. Translation: BAA24840.1 .
    U65637 mRNA. Translation: AAC28776.1 .
    AF090137 mRNA. Translation: AAD19878.1 .
    AY339617 Genomic DNA. Translation: AAP88041.1 .
    CH471064 Genomic DNA. Translation: EAW68035.1 .
    CH471064 Genomic DNA. Translation: EAW68038.1 .
    BC022567 mRNA. Translation: AAH22567.1 .
    BC028235 mRNA. Translation: AAH28235.1 .
    CCDSi CCDS7913.1.
    RefSeqi NP_003645.1. NM_003654.5.
    UniGenei Hs.104576.

    3D structure databases

    ProteinModelPortali O43916.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 114104. 2 interactions.
    IntActi O43916. 2 interactions.
    MINTi MINT-1440600.
    STRINGi 9606.ENSP00000309270.

    PTM databases

    PhosphoSitei O43916.

    Proteomic databases

    PaxDbi O43916.
    PRIDEi O43916.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000308064 ; ENSP00000309270 ; ENSG00000175264 .
    GeneIDi 8534.
    KEGGi hsa:8534.
    UCSCi uc001mys.2. human.

    Organism-specific databases

    CTDi 8534.
    GeneCardsi GC11M045670.
    HGNCi HGNC:1969. CHST1.
    MIMi 603797. gene.
    neXtProti NX_O43916.
    PharmGKBi PA26501.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG80862.
    HOGENOMi HOG000261614.
    HOVERGENi HBG106811.
    InParanoidi O43916.
    KOi K01022.
    OMAi CEEGPTF.
    OrthoDBi EOG7RZ5S0.
    PhylomeDBi O43916.
    TreeFami TF342871.

    Enzyme and pathway databases

    BRENDAi 2.8.2.21. 2681.
    Reactomei REACT_121120. Keratan sulfate biosynthesis.
    SABIO-RK O43916.

    Miscellaneous databases

    GeneWikii CHST1.
    GenomeRNAii 8534.
    NextBioi 31964.
    PROi O43916.
    SOURCEi Search...

    Gene expression databases

    Bgeei O43916.
    CleanExi HS_CHST1.
    Genevestigatori O43916.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR016469. Carbohydrate_sulfotransferase.
    IPR027417. P-loop_NTPase.
    IPR000863. Sulfotransferase_dom.
    [Graphical view ]
    Pfami PF00685. Sulfotransfer_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
    SUPFAMi SSF52540. SSF52540. 2 hits.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase."
      Fukuta M., Inazawa J., Torii T., Tsuzuki K., Shimada E., Habuchi O.
      J. Biol. Chem. 272:32321-32328(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
      Tissue: Fetal brain.
    2. "Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization."
      Mazany K.D., Peng T., Watson C.E., Tabas I., Williams K.J.
      Biochim. Biophys. Acta 1407:92-97(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization."
      Li X., Tedder T.F.
      Genomics 55:345-347(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Umbilical vein endothelial cell.
    4. NIEHS SNPs program
      Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    7. "Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase."
      Torii T., Fukuta M., Habuchi O.
      Glycobiology 10:203-211(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBSTRATE SPECIFICITY, FUNCTION.
    8. "Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin."
      Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S.
      J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. "CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin."
      Li X., Tu L., Murphy P.G., Kadono T., Steeber D.A., Tedder T.F.
      J. Leukoc. Biol. 69:565-574(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiCHST1_HUMAN
    AccessioniPrimary (citable) accession number: O43916
    Secondary accession number(s): D3DQP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 111 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Caution

    Was originally (PubMed:9639683) thought to be the ortholog of chicken CHST3 and therefore named C6ST. However, it has no strong chondroitin 6-sulfotransferase activity.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3