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Protein

Carbohydrate sulfotransferase 1

Gene

CHST1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation.2 Publications

Caution

Was originally (PubMed:9639683) thought to be the ortholog of chicken CHST3 and therefore named C6ST. However, it has no strong chondroitin 6-sulfotransferase activity.1 Publication

Catalytic activityi

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi69 – 75PAPSBy similarity7
Nucleotide bindingi234 – 242PAPSBy similarity9

GO - Molecular functioni

  • keratan sulfotransferase activity Source: UniProtKB
  • N-acetylglucosamine 6-O-sulfotransferase activity Source: GO_Central
  • sulfotransferase activity Source: UniProtKB

GO - Biological processi

  • galactose metabolic process Source: UniProtKB
  • inflammatory response Source: UniProtKB-KW
  • keratan sulfate biosynthetic process Source: Reactome
  • keratan sulfate metabolic process Source: UniProtKB
  • polysaccharide metabolic process Source: ProtInc
  • sulfur compound metabolic process Source: UniProtKB

Keywordsi

Molecular functionTransferase
Biological processCarbohydrate metabolism, Inflammatory response

Enzyme and pathway databases

BioCyciMetaCyc:HS10902-MONOMER
BRENDAi2.8.2.21 2681
ReactomeiR-HSA-2022854 Keratan sulfate biosynthesis
SABIO-RKO43916

Names & Taxonomyi

Protein namesi
Recommended name:
Carbohydrate sulfotransferase 1 (EC:2.8.2.21)
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name:
GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name:
KS6ST
Short name:
KSGal6ST
Short name:
KSST
Gene namesi
Name:CHST1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 11

Organism-specific databases

EuPathDBiHostDB:ENSG00000175264.7
HGNCiHGNC:1969 CHST1
MIMi603797 gene
neXtProtiNX_O43916

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 2CytoplasmicSequence analysis2
Transmembranei3 – 23Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini24 – 411LumenalSequence analysisAdd BLAST388

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi8534
OpenTargetsiENSG00000175264
PharmGKBiPA26501

Polymorphism and mutation databases

BioMutaiCHST1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000851821 – 411Carbohydrate sulfotransferase 1Add BLAST411

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi56N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi145N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi189N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi334N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiO43916
PeptideAtlasiO43916
PRIDEiO43916

PTM databases

iPTMnetiO43916
PhosphoSitePlusiO43916

Expressioni

Tissue specificityi

Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes.2 Publications

Gene expression databases

BgeeiENSG00000175264
CleanExiHS_CHST1
GenevisibleiO43916 HS

Interactioni

Protein-protein interaction databases

BioGridi114104, 3 interactors
IntActiO43916, 2 interactors
STRINGi9606.ENSP00000309270

Structurei

3D structure databases

ProteinModelPortaliO43916
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi337 – 339Cell attachment siteSequence analysis3

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IHC0 Eukaryota
ENOG4110V0B LUCA
GeneTreeiENSGT00530000062902
HOGENOMiHOG000261614
HOVERGENiHBG106811
InParanoidiO43916
KOiK01022
OMAiCEEGPTF
OrthoDBiEOG091G0V3Y
PhylomeDBiO43916
TreeFamiTF342871

Family and domain databases

InterProiView protein in InterPro
IPR016469 Carbohydrate_sulfotransferase
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PfamiView protein in Pfam
PF00685 Sulfotransfer_1, 1 hit
PIRSFiPIRSF005883 Carbohydrate_sulfotransferase, 1 hit
SUPFAMiSSF52540 SSF52540, 2 hits

Sequencei

Sequence statusi: Complete.

O43916-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLAEA GLAERLCEES
60 70 80 90 100
PTFAYNLSRK THILILATTR SGSSFVGQLF NQHLDVFYLF EPLYHVQNTL
110 120 130 140 150
IPRFTQGKSP ADRRVMLGAS RDLLRSLYDC DLYFLENYIK PPPVNHTTDR
160 170 180 190 200
IFRRGASRVL CSRPVCDPPG PADLVLEEGD CVRKCGLLNL TVAAEACRER
210 220 230 240 250
SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL ASRSETFRDT
260 270 280 290 300
YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV
310 320 330 340 350
RYEDLARNPM KKTEEIYGFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV
360 370 380 390 400
RNSAATAEKW RFRLSYDIVA FAQNACQQVL AQLGYKIAAS EEELKNPSVS
410
LVEERDFRPF S
Length:411
Mass (Da):46,715
Last modified:June 1, 1998 - v1
Checksum:iF07D0A23B6338A09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB003791 mRNA Translation: BAA24840.1
U65637 mRNA Translation: AAC28776.1
AF090137 mRNA Translation: AAD19878.1
AY339617 Genomic DNA Translation: AAP88041.1
CH471064 Genomic DNA Translation: EAW68035.1
CH471064 Genomic DNA Translation: EAW68038.1
BC022567 mRNA Translation: AAH22567.1
BC028235 mRNA Translation: AAH28235.1
CCDSiCCDS7913.1
RefSeqiNP_003645.1, NM_003654.5
UniGeneiHs.104576

Genome annotation databases

EnsembliENST00000308064; ENSP00000309270; ENSG00000175264
GeneIDi8534
KEGGihsa:8534
UCSCiuc001mys.3 human

Similar proteinsi

Entry informationi

Entry nameiCHST1_HUMAN
AccessioniPrimary (citable) accession number: O43916
Secondary accession number(s): D3DQP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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