Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

O43916 (CHST1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Carbohydrate sulfotransferase 1

EC=2.8.2.21
Alternative name(s):
Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 1
Short name=GST-1
Keratan sulfate Gal-6 sulfotransferase
Short name=KS6ST
Short name=KSGal6ST
Short name=KSST
Gene names
Name:CHST1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length411 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of galactose (Gal) residues of keratan. Has a preference for sulfating keratan sulfate, but it also transfers sulfate to the unsulfated polymer. The sulfotransferase activity on sialyl LacNAc structures is much higher than the corresponding desialylated substrate, and only internal Gal residues are sulfated. May function in the sulfation of sialyl N-acetyllactosamine oligosaccharide chains attached to glycoproteins. Participates in biosynthesis of selectin ligands. Selectin ligands are present in high endothelial cells (HEVs) and play a central role in lymphocyte homing at sites of inflammation. Ref.7 Ref.8

Catalytic activity

3'-phosphoadenylyl sulfate + keratan = adenosine 3',5'-bisphosphate + keratan 6'-sulfate. Ref.1

Subcellular location

Golgi apparatus membrane; Single-pass type II membrane protein By similarity.

Tissue specificity

Widely expressed at low level. Expressed in brain and skeletal muscle. Expressed by high endothelial cells (HEVs) and leukocytes. Ref.1 Ref.9

Sequence similarities

Belongs to the sulfotransferase 1 family. Gal/GlcNAc/GalNAc subfamily.

Caution

Was originally (Ref.2) thought to be the ortholog of chicken CHST3 and therefore named C6ST. However, it has no strong chondroitin 6-sulfotransferase activity.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 411411Carbohydrate sulfotransferase 1
PRO_0000085182

Regions

Topological domain1 – 22Cytoplasmic Potential
Transmembrane3 – 2321Helical; Signal-anchor for type II membrane protein; Potential
Topological domain24 – 411388Lumenal Potential
Nucleotide binding69 – 757PAPS By similarity
Nucleotide binding234 – 2429PAPS By similarity
Motif337 – 3393Cell attachment site Potential

Amino acid modifications

Glycosylation561N-linked (GlcNAc...) Potential
Glycosylation1451N-linked (GlcNAc...) Potential
Glycosylation1891N-linked (GlcNAc...) Potential
Glycosylation3341N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
O43916 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: F07D0A23B6338A09

FASTA41146,715
        10         20         30         40         50         60 
MQCSWKAVLL LALASIAIQY TAIRTFTAKS FHTCPGLAEA GLAERLCEES PTFAYNLSRK 

        70         80         90        100        110        120 
THILILATTR SGSSFVGQLF NQHLDVFYLF EPLYHVQNTL IPRFTQGKSP ADRRVMLGAS 

       130        140        150        160        170        180 
RDLLRSLYDC DLYFLENYIK PPPVNHTTDR IFRRGASRVL CSRPVCDPPG PADLVLEEGD 

       190        200        210        220        230        240 
CVRKCGLLNL TVAAEACRER SHVAIKTVRV PEVNDLRALV EDPRLNLKVI QLVRDPRGIL 

       250        260        270        280        290        300 
ASRSETFRDT YRLWRLWYGT GRKPYNLDVT QLTTVCEDFS NSVSTGLMRP PWLKGKYMLV 

       310        320        330        340        350        360 
RYEDLARNPM KKTEEIYGFL GIPLDSHVAR WIQNNTRGDP TLGKHKYGTV RNSAATAEKW 

       370        380        390        400        410 
RFRLSYDIVA FAQNACQQVL AQLGYKIAAS EEELKNPSVS LVEERDFRPF S 

« Hide

References

« Hide 'large scale' references
[1]"Molecular cloning and characterization of human keratan sulfate Gal-6-sulfotransferase."
Fukuta M., Inazawa J., Torii T., Tsuzuki K., Shimada E., Habuchi O.
J. Biol. Chem. 272:32321-32328(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, TISSUE SPECIFICITY.
Tissue: Fetal brain.
[2]"Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization."
Mazany K.D., Peng T., Watson C.E., Tabas I., Williams K.J.
Biochim. Biophys. Acta 1407:92-97(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"CHST1 and CHST2 sulfotransferases expressed by human vascular endothelial cells: cDNA cloning, expression, and chromosomal localization."
Li X., Tedder T.F.
Genomics 55:345-347(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Umbilical vein endothelial cell.
[4]NIEHS SNPs program
Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[5]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Brain.
[7]"Sulfation of sialyl N-acetyllactosamine oligosaccharides and fetuin oligosaccharides by keratan sulfate Gal-6-sulfotransferase."
Torii T., Fukuta M., Habuchi O.
Glycobiology 10:203-211(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBSTRATE SPECIFICITY, FUNCTION.
[8]"Sulfotransferases of two specificities function in the reconstitution of high endothelial cell ligands for L-selectin."
Bistrup A., Bhakta S., Lee J.K., Belov Y.Y., Gunn M.D., Zuo F.-R., Huang C.-C., Kannagi R., Rosen S.D., Hemmerich S.
J. Cell Biol. 145:899-910(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"CHST1 and CHST2 sulfotransferase expression by vascular endothelial cells regulates shear-resistant leukocyte rolling via L-selectin."
Li X., Tu L., Murphy P.G., Kadono T., Steeber D.A., Tedder T.F.
J. Leukoc. Biol. 69:565-574(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Web resources

GGDB

GlycoGene database

NIEHS-SNPs

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AB003791 mRNA. Translation: BAA24840.1.
U65637 mRNA. Translation: AAC28776.1.
AF090137 mRNA. Translation: AAD19878.1.
AY339617 Genomic DNA. Translation: AAP88041.1.
CH471064 Genomic DNA. Translation: EAW68035.1.
CH471064 Genomic DNA. Translation: EAW68038.1.
BC022567 mRNA. Translation: AAH22567.1.
BC028235 mRNA. Translation: AAH28235.1.
CCDSCCDS7913.1.
RefSeqNP_003645.1. NM_003654.5.
UniGeneHs.104576.

3D structure databases

ProteinModelPortalO43916.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid114104. 2 interactions.
IntActO43916. 2 interactions.
MINTMINT-1440600.
STRING9606.ENSP00000309270.

PTM databases

PhosphoSiteO43916.

Proteomic databases

PaxDbO43916.
PRIDEO43916.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000308064; ENSP00000309270; ENSG00000175264.
GeneID8534.
KEGGhsa:8534.
UCSCuc001mys.2. human.

Organism-specific databases

CTD8534.
GeneCardsGC11M045670.
HGNCHGNC:1969. CHST1.
MIM603797. gene.
neXtProtNX_O43916.
PharmGKBPA26501.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG80862.
HOGENOMHOG000261614.
HOVERGENHBG106811.
InParanoidO43916.
KOK01022.
OMACEEGPTF.
OrthoDBEOG7RZ5S0.
PhylomeDBO43916.
TreeFamTF342871.

Enzyme and pathway databases

BRENDA2.8.2.21. 2681.
ReactomeREACT_111217. Metabolism.
REACT_116125. Disease.
SABIO-RKO43916.

Gene expression databases

BgeeO43916.
CleanExHS_CHST1.
GenevestigatorO43916.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR016469. Carbohydrate_sulfotransferase.
IPR027417. P-loop_NTPase.
IPR000863. Sulfotransferase_dom.
[Graphical view]
PfamPF00685. Sulfotransfer_1. 1 hit.
[Graphical view]
PIRSFPIRSF005883. Carbohydrate_sulfotransferase. 1 hit.
SUPFAMSSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

GeneWikiCHST1.
GenomeRNAi8534.
NextBio31964.
PROO43916.
SOURCESearch...

Entry information

Entry nameCHST1_HUMAN
AccessionPrimary (citable) accession number: O43916
Secondary accession number(s): D3DQP2
Entry history
Integrated into UniProtKB/Swiss-Prot: March 15, 2005
Last sequence update: June 1, 1998
Last modified: July 9, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM