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O43913

- ORC5_HUMAN

UniProt

O43913 - ORC5_HUMAN

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Protein
Origin recognition complex subunit 5
Gene
ORC5, ORC5L
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi37 – 448ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. DNA replication origin binding Source: ProtInc
  3. nucleotide binding Source: ProtInc
  4. protein binding Source: IntAct

GO - Biological processi

  1. DNA replication Source: UniProtKB
  2. DNA replication initiation Source: ProtInc
  3. G1/S transition of mitotic cell cycle Source: Reactome
  4. mitotic cell cycle Source: Reactome
Complete GO annotation...

Keywords - Biological processi

DNA replication

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_1095. Activation of the pre-replicative complex.
REACT_1156. Orc1 removal from chromatin.
REACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_207. Removal of licensing factors from origins.
REACT_2243. Assembly of the pre-replicative complex.
REACT_567. Assembly of the ORC complex at the origin of replication.
REACT_6769. Activation of ATR in response to replication stress.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 5
Gene namesi
Name:ORC5
Synonyms:ORC5L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:8491. ORC5.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: HPA
  2. nuclear origin of replication recognition complex Source: UniProtKB
  3. nucleoplasm Source: Reactome
  4. nucleus Source: UniProtKB
  5. origin recognition complex Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA32812.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 435435Origin recognition complex subunit 5
PRO_0000127092Add
BLAST

Proteomic databases

MaxQBiO43913.
PaxDbiO43913.
PeptideAtlasiO43913.
PRIDEiO43913.

PTM databases

PhosphoSiteiO43913.

Expressioni

Tissue specificityi

Abundant in spleen, ovary, prostate, testis, and colon mucosa.1 Publication

Gene expression databases

ArrayExpressiO43913.
BgeeiO43913.
CleanExiHS_ORC5L.
GenevestigatoriO43913.

Organism-specific databases

HPAiHPA019730.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ORC1Q134155EBI-374928,EBI-374847
ORC2Q134167EBI-374928,EBI-374957
ORC3Q9UBD515EBI-374928,EBI-374916
ORC4O439292EBI-374928,EBI-374889

Protein-protein interaction databases

BioGridi111043. 34 interactions.
DIPiDIP-29691N.
IntActiO43913. 23 interactions.
MINTiMINT-1201309.

Structurei

3D structure databases

ProteinModelPortaliO43913.
SMRiO43913. Positions 32-59.

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC5 family.

Phylogenomic databases

eggNOGiNOG249220.
HOGENOMiHOG000252907.
HOVERGENiHBG007875.
InParanoidiO43913.
KOiK02607.
OMAiVPCRESQ.
OrthoDBiEOG7DZ8K4.
PhylomeDBiO43913.
TreeFamiTF101095.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR020796. ORC5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR12705. PTHR12705. 1 hit.
PfamiPF14630. ORC5_C. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O43913-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL    50
LKTLELPHVF VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN 100
DFVRLFKQVT TAENLKDQTV YIVLDKAEYL RDMEANLLPG FLRLQELADR 150
NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS 200
ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEASERDT 250
RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV 300
ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH 350
LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD 400
QLDGPKYKCT VSLDFIRAIA RTVNFDIIKY LYDFL 435
Length:435
Mass (Da):50,283
Last modified:June 1, 1998 - v1
Checksum:i0FEB0E829C124745
GO
Isoform 2 (identifier: O43913-2) [UniParc]FASTAAdd to Basket

Also known as: ORC5T

The sequence of this isoform differs from the canonical sequence as follows:
     294-435: LSAHTHVELP...DIIKYLYDFL → IRGSIETVTGDTSSANYQDTKVNTKEHSVRK

Note: Does not interact with ORC2.

Show »
Length:324
Mass (Da):37,414
Checksum:i005B2887929C62C8
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti37 – 371G → R.1 Publication
Corresponds to variant rs1056677 [ dbSNP | Ensembl ].
VAR_011800
Natural varianti52 – 521K → N.
Corresponds to variant rs2307413 [ dbSNP | Ensembl ].
VAR_014524
Natural varianti166 – 1661R → C.
Corresponds to variant rs2307402 [ dbSNP | Ensembl ].
VAR_014525

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei294 – 435142LSAHT…LYDFL → IRGSIETVTGDTSSANYQDT KVNTKEHSVRK in isoform 2.
VSP_042037Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF047599 mRNA. Translation: AAC80283.1.
U92538 mRNA. Translation: AAC51933.1.
AF049127 mRNA. Translation: AAC63972.1.
AF081459 mRNA. Translation: AAC64401.1.
AC002067 Genomic DNA. No translation available.
AC007393 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24409.1.
CH471070 Genomic DNA. Translation: EAW83341.1.
BC023652 mRNA. Translation: AAH23652.1.
CCDSiCCDS47681.1. [O43913-2]
CCDS5734.1. [O43913-1]
RefSeqiNP_002544.1. NM_002553.3. [O43913-1]
NP_859531.1. NM_181747.3. [O43913-2]
UniGeneiHs.432948.

Genome annotation databases

EnsembliENST00000297431; ENSP00000297431; ENSG00000164815. [O43913-1]
ENST00000447452; ENSP00000395747; ENSG00000164815. [O43913-2]
GeneIDi5001.
KEGGihsa:5001.
UCSCiuc003vcb.3. human. [O43913-1]
uc003vcc.3. human. [O43913-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF047599 mRNA. Translation: AAC80283.1 .
U92538 mRNA. Translation: AAC51933.1 .
AF049127 mRNA. Translation: AAC63972.1 .
AF081459 mRNA. Translation: AAC64401.1 .
AC002067 Genomic DNA. No translation available.
AC007393 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24409.1 .
CH471070 Genomic DNA. Translation: EAW83341.1 .
BC023652 mRNA. Translation: AAH23652.1 .
CCDSi CCDS47681.1. [O43913-2 ]
CCDS5734.1. [O43913-1 ]
RefSeqi NP_002544.1. NM_002553.3. [O43913-1 ]
NP_859531.1. NM_181747.3. [O43913-2 ]
UniGenei Hs.432948.

3D structure databases

ProteinModelPortali O43913.
SMRi O43913. Positions 32-59.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111043. 34 interactions.
DIPi DIP-29691N.
IntActi O43913. 23 interactions.
MINTi MINT-1201309.

PTM databases

PhosphoSitei O43913.

Proteomic databases

MaxQBi O43913.
PaxDbi O43913.
PeptideAtlasi O43913.
PRIDEi O43913.

Protocols and materials databases

DNASUi 5001.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000297431 ; ENSP00000297431 ; ENSG00000164815 . [O43913-1 ]
ENST00000447452 ; ENSP00000395747 ; ENSG00000164815 . [O43913-2 ]
GeneIDi 5001.
KEGGi hsa:5001.
UCSCi uc003vcb.3. human. [O43913-1 ]
uc003vcc.3. human. [O43913-2 ]

Organism-specific databases

CTDi 5001.
GeneCardsi GC07M103767.
HGNCi HGNC:8491. ORC5.
HPAi HPA019730.
MIMi 602331. gene.
neXtProti NX_O43913.
PharmGKBi PA32812.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG249220.
HOGENOMi HOG000252907.
HOVERGENi HBG007875.
InParanoidi O43913.
KOi K02607.
OMAi VPCRESQ.
OrthoDBi EOG7DZ8K4.
PhylomeDBi O43913.
TreeFami TF101095.

Enzyme and pathway databases

Reactomei REACT_1095. Activation of the pre-replicative complex.
REACT_1156. Orc1 removal from chromatin.
REACT_1181. Association of licensing factors with the pre-replicative complex.
REACT_1321. E2F-enabled inhibition of pre-replication complex formation.
REACT_1707. CDC6 association with the ORC:origin complex.
REACT_1949. CDT1 association with the CDC6:ORC:origin complex.
REACT_207. Removal of licensing factors from origins.
REACT_2243. Assembly of the pre-replicative complex.
REACT_567. Assembly of the ORC complex at the origin of replication.
REACT_6769. Activation of ATR in response to replication stress.

Miscellaneous databases

GeneWikii ORC5.
ORC5L.
GenomeRNAii 5001.
NextBioi 19256.
PROi O43913.
SOURCEi Search...

Gene expression databases

ArrayExpressi O43913.
Bgeei O43913.
CleanExi HS_ORC5L.
Genevestigatori O43913.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR020796. ORC5.
IPR027417. P-loop_NTPase.
[Graphical view ]
PANTHERi PTHR12705. PTHR12705. 1 hit.
Pfami PF14630. ORC5_C. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p."
    Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B.
    J. Biol. Chem. 273:32421-32429(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  2. "Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22."
    Ishiai M., Dean F.B., Okumura K., Abe M., Moon K.-Y., Amin A.A., Kagotani K., Taguchi H., Murakami Y., Hanaoka F., O'Donnell M., Hurwitz J., Eki T.
    Genomics 46:294-298(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases."
    Quintana D.G., Thome K.C., Hou Z.-H., Ligon A.H., Morton C.C., Dutta A.
    J. Biol. Chem. 273:27137-27145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANT ARG-37.
  4. "The DNA sequence of human chromosome 7."
    Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
    , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
    Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Uterus.
  8. "The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle."
    Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C.
    J. Biol. Chem. 278:41535-41540(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ORC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX.
  9. "ATP-dependent assembly of the human origin recognition complex."
    Siddiqui K., Stillman B.
    J. Biol. Chem. 282:32370-32383(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX.
  10. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiORC5_HUMAN
AccessioniPrimary (citable) accession number: O43913
Secondary accession number(s): A4D0P8, O60590, O95268
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: September 3, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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