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O43913 (ORC5_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Origin recognition complex subunit 5
Gene names
Name:ORC5
Synonyms:ORC5L
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length435 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Subunit structure

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase. Ref.8

Subcellular location

Nucleus.

Tissue specificity

Abundant in spleen, ovary, prostate, testis, and colon mucosa. Ref.3

Sequence similarities

Belongs to the ORC5 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O43913-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O43913-2)

Also known as: ORC5T;

The sequence of this isoform differs from the canonical sequence as follows:
     294-435: LSAHTHVELP...DIIKYLYDFL → IRGSIETVTGDTSSANYQDTKVNTKEHSVRK
Note: Does not interact with ORC2.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 435435Origin recognition complex subunit 5
PRO_0000127092

Regions

Nucleotide binding37 – 448ATP Potential

Natural variations

Alternative sequence294 – 435142LSAHT…LYDFL → IRGSIETVTGDTSSANYQDT KVNTKEHSVRK in isoform 2.
VSP_042037
Natural variant371G → R. Ref.3
Corresponds to variant rs1056677 [ dbSNP | Ensembl ].
VAR_011800
Natural variant521K → N.
Corresponds to variant rs2307413 [ dbSNP | Ensembl ].
VAR_014524
Natural variant1661R → C.
Corresponds to variant rs2307402 [ dbSNP | Ensembl ].
VAR_014525

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified June 1, 1998. Version 1.
Checksum: 0FEB0E829C124745

FASTA43550,283
        10         20         30         40         50         60 
MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL LKTLELPHVF 

        70         80         90        100        110        120 
VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN DFVRLFKQVT TAENLKDQTV 

       130        140        150        160        170        180 
YIVLDKAEYL RDMEANLLPG FLRLQELADR NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP 

       190        200        210        220        230        240 
DYSIGNLQKI LSHDHPPEYS ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV 

       250        260        270        280        290        300 
VKGEASERDT RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV 

       310        320        330        340        350        360 
ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH LLGPKPFPLD 

       370        380        390        400        410        420 
RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD QLDGPKYKCT VSLDFIRAIA 

       430 
RTVNFDIIKY LYDFL 

« Hide

Isoform 2 (ORC5T) [UniParc].

Checksum: 005B2887929C62C8
Show »

FASTA32437,414

References

« Hide 'large scale' references
[1]"The Orc4p and Orc5p subunits of the Xenopus and human origin recognition complex are related to Orc1p and Cdc6p."
Tugal T., Zou-Yang X.H., Gavin K., Pappin D., Canas B., Kobayashi R., Hunt T., Stillman B.
J. Biol. Chem. 273:32421-32429(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[2]"Isolation of human and fission yeast homologues of the budding yeast origin recognition complex subunit ORC5: human homologue (ORC5L) maps to 7q22."
Ishiai M., Dean F.B., Okumura K., Abe M., Moon K.-Y., Amin A.A., Kagotani K., Taguchi H., Murakami Y., Hanaoka F., O'Donnell M., Hurwitz J., Eki T.
Genomics 46:294-298(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"ORC5L, a new member of the human origin recognition complex, is deleted in uterine leiomyomas and malignant myeloid diseases."
Quintana D.G., Thome K.C., Hou Z.-H., Ligon A.H., Morton C.C., Dutta A.
J. Biol. Chem. 273:27137-27145(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE SPLICING, TISSUE SPECIFICITY, VARIANT ARG-37.
[4]"The DNA sequence of human chromosome 7."
Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. expand/collapse author list , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"Human chromosome 7: DNA sequence and biology."
Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. expand/collapse author list , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Uterus.
[8]"The ORC1 cycle in human cells: II. Dynamic changes in the human ORC complex during the cell cycle."
Ohta S., Tatsumi Y., Fujita M., Tsurimoto T., Obuse C.
J. Biol. Chem. 278:41535-41540(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ORC COMPLEX, IDENTIFICATION BY MASS SPECTROMETRY, ASSEMBLY OF THE ORC COMPLEX.
[9]"ATP-dependent assembly of the human origin recognition complex."
Siddiqui K., Stillman B.
J. Biol. Chem. 282:32370-32383(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: RECONSTITUTION OF THE ORC COMPLEX, DISASSEMBLY OF THE ORC COMPLEX.
[10]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF047599 mRNA. Translation: AAC80283.1.
U92538 mRNA. Translation: AAC51933.1.
AF049127 mRNA. Translation: AAC63972.1.
AF081459 mRNA. Translation: AAC64401.1.
AC002067 Genomic DNA. No translation available.
AC007393 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24409.1.
CH471070 Genomic DNA. Translation: EAW83341.1.
BC023652 mRNA. Translation: AAH23652.1.
RefSeqNP_002544.1. NM_002553.3.
NP_859531.1. NM_181747.3.
UniGeneHs.432948.

3D structure databases

ProteinModelPortalO43913.
SMRO43913. Positions 29-61.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid111043. 34 interactions.
DIPDIP-29691N.
IntActO43913. 23 interactions.
MINTMINT-1201309.

PTM databases

PhosphoSiteO43913.

Proteomic databases

PaxDbO43913.
PeptideAtlasO43913.
PRIDEO43913.

Protocols and materials databases

DNASU5001.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000297431; ENSP00000297431; ENSG00000164815. [O43913-1]
ENST00000447452; ENSP00000395747; ENSG00000164815. [O43913-2]
GeneID5001.
KEGGhsa:5001.
UCSCuc003vcb.3. human. [O43913-1]
uc003vcc.3. human. [O43913-2]

Organism-specific databases

CTD5001.
GeneCardsGC07M103767.
HGNCHGNC:8491. ORC5.
HPAHPA019730.
MIM602331. gene.
neXtProtNX_O43913.
PharmGKBPA32812.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG249220.
HOGENOMHOG000252907.
HOVERGENHBG007875.
InParanoidO43913.
KOK02607.
OMAVPCRESQ.
OrthoDBEOG7DZ8K4.
PhylomeDBO43913.
TreeFamTF101095.

Enzyme and pathway databases

ReactomeREACT_115566. Cell Cycle.
REACT_21300. Mitotic M-M/G1 phases.
REACT_383. DNA Replication.

Gene expression databases

ArrayExpressO43913.
BgeeO43913.
CleanExHS_ORC5L.
GenevestigatorO43913.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR020796. ORC5.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR12705. PTHR12705. 1 hit.
PfamPF14630. ORC5_C. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

GeneWikiORC5.
ORC5L.
GenomeRNAi5001.
NextBio19256.
PROO43913.
SOURCESearch...

Entry information

Entry nameORC5_HUMAN
AccessionPrimary (citable) accession number: O43913
Secondary accession number(s): A4D0P8, O60590, O95268
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM