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Protein

Origin recognition complex subunit 5

Gene

ORC5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi37 – 44ATPSequence analysis8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • DNA replication origin binding Source: ProtInc
  • nucleotide binding Source: ProtInc

GO - Biological processi

  • DNA replication Source: Reactome
  • DNA replication initiation Source: ProtInc
  • G1/S transition of mitotic cell cycle Source: Reactome

Keywordsi

Biological processDNA replication
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-113507. E2F-enabled inhibition of pre-replication complex formation.
R-HSA-176187. Activation of ATR in response to replication stress.
R-HSA-68616. Assembly of the ORC complex at the origin of replication.
R-HSA-68689. CDC6 association with the ORC:origin complex.
R-HSA-68827. CDT1 association with the CDC6:ORC:origin complex.
R-HSA-68867. Assembly of the pre-replicative complex.
R-HSA-68949. Orc1 removal from chromatin.
R-HSA-68962. Activation of the pre-replicative complex.
R-HSA-69298. Association of licensing factors with the pre-replicative complex.
R-HSA-69300. Removal of licensing factors from origins.

Names & Taxonomyi

Protein namesi
Recommended name:
Origin recognition complex subunit 5
Gene namesi
Name:ORC5
Synonyms:ORC5L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:8491. ORC5.

Subcellular locationi

GO - Cellular componenti

  • cytosol Source: HPA
  • nuclear chromosome, telomeric region Source: BHF-UCL
  • nuclear origin of replication recognition complex Source: UniProtKB
  • nucleoplasm Source: Reactome
  • nucleus Source: HPA
  • origin recognition complex Source: UniProtKB

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

DisGeNETi5001.
OpenTargetsiENSG00000164815.
PharmGKBiPA32812.

Polymorphism and mutation databases

BioMutaiORC5.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001270921 – 435Origin recognition complex subunit 5Add BLAST435

Proteomic databases

EPDiO43913.
MaxQBiO43913.
PaxDbiO43913.
PeptideAtlasiO43913.
PRIDEiO43913.

PTM databases

iPTMnetiO43913.
PhosphoSitePlusiO43913.

Expressioni

Tissue specificityi

Abundant in spleen, ovary, prostate, testis, and colon mucosa.1 Publication

Gene expression databases

BgeeiENSG00000164815.
CleanExiHS_ORC5L.
ExpressionAtlasiO43913. baseline and differential.
GenevisibleiO43913. HS.

Organism-specific databases

HPAiHPA019730.

Interactioni

Subunit structurei

Component of ORC, a complex composed of at least 6 subunits: ORC1, ORC2, ORC3, ORC4, ORC5 and ORC6. ORC is regulated in a cell-cycle dependent manner. It is sequentially assembled at the exit from anaphase of mitosis and disassembled as cells enter S phase.1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi111043. 50 interactors.
DIPiDIP-29691N.
IntActiO43913. 26 interactors.
MINTiMINT-1201309.
STRINGi9606.ENSP00000297431.

Structurei

Secondary structure

1435
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi12 – 22Combined sources11
Beta strandi31 – 36Combined sources6
Helixi39 – 41Combined sources3
Helixi43 – 53Combined sources11
Beta strandi58 – 62Combined sources5
Turni63 – 65Combined sources3
Helixi69 – 78Combined sources10
Helixi100 – 108Combined sources9
Turni113 – 115Combined sources3
Beta strandi116 – 118Combined sources3
Beta strandi120 – 125Combined sources6
Helixi127 – 132Combined sources6
Helixi137 – 142Combined sources6
Helixi144 – 148Combined sources5
Beta strandi152 – 160Combined sources9
Helixi162 – 164Combined sources3
Beta strandi175 – 178Combined sources4
Helixi184 – 191Combined sources8
Turni192 – 194Combined sources3
Helixi201 – 215Combined sources15
Turni216 – 218Combined sources3
Helixi222 – 231Combined sources10
Helixi233 – 241Combined sources9
Helixi254 – 265Combined sources12

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5UJ7X-ray3.39E/F1-284[»]
5UJMelectron microscopy18.00E1-435[»]
ProteinModelPortaliO43913.
SMRiO43913.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ORC5 family.Curated

Phylogenomic databases

eggNOGiKOG2543. Eukaryota.
ENOG410XSY0. LUCA.
GeneTreeiENSGT00390000009380.
HOGENOMiHOG000252907.
HOVERGENiHBG007875.
InParanoidiO43913.
KOiK02607.
OMAiGEQNIMD.
OrthoDBiEOG091G0D0I.
PhylomeDBiO43913.
TreeFamiTF101095.

Family and domain databases

InterProiView protein in InterPro
IPR020796. ORC5.
IPR027417. P-loop_NTPase.
PANTHERiPTHR12705. PTHR12705. 1 hit.
PfamiView protein in Pfam
PF14630. ORC5_C. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43913-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPHLENVVLC RESQVSILQS LFGERHHFSF PSIFIYGHTA SGKTYVTQTL
60 70 80 90 100
LKTLELPHVF VNCVECFTLR LLLEQILNKL NHLSSSEDGC STEITCETFN
110 120 130 140 150
DFVRLFKQVT TAENLKDQTV YIVLDKAEYL RDMEANLLPG FLRLQELADR
160 170 180 190 200
NVTVLFLSEI VWEKFRPNTG CFEPFVLYFP DYSIGNLQKI LSHDHPPEYS
210 220 230 240 250
ADFYAAYINI LLGVFYTVCR DLKELRHLAV LNFPKYCEPV VKGEASERDT
260 270 280 290 300
RKLWRNIEPH LKKAMQTVYL REISSSQWEK LQKDDTDPGQ LKGLSAHTHV
310 320 330 340 350
ELPYYSKFIL IAAYLASYNP ARTDKRFFLK HHGKIKKTNF LKKHEKTSNH
360 370 380 390 400
LLGPKPFPLD RLLAILYSIV DSRVAPTANI FSQITSLVTL QLLTLVGHDD
410 420 430
QLDGPKYKCT VSLDFIRAIA RTVNFDIIKY LYDFL
Length:435
Mass (Da):50,283
Last modified:June 1, 1998 - v1
Checksum:i0FEB0E829C124745
GO
Isoform 2 (identifier: O43913-2) [UniParc]FASTAAdd to basket
Also known as: ORC5T

The sequence of this isoform differs from the canonical sequence as follows:
     294-435: LSAHTHVELP...DIIKYLYDFL → IRGSIETVTGDTSSANYQDTKVNTKEHSVRK

Note: Does not interact with ORC2.
Show »
Length:324
Mass (Da):37,414
Checksum:i005B2887929C62C8
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_01180037G → R1 PublicationCorresponds to variant dbSNP:rs1056677Ensembl.1
Natural variantiVAR_01452452K → N. Corresponds to variant dbSNP:rs2307413Ensembl.1
Natural variantiVAR_014525166R → C. Corresponds to variant dbSNP:rs2307402Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_042037294 – 435LSAHT…LYDFL → IRGSIETVTGDTSSANYQDT KVNTKEHSVRK in isoform 2. 1 PublicationAdd BLAST142

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047599 mRNA. Translation: AAC80283.1.
U92538 mRNA. Translation: AAC51933.1.
AF049127 mRNA. Translation: AAC63972.1.
AF081459 mRNA. Translation: AAC64401.1.
AC002067 Genomic DNA. No translation available.
AC007393 Genomic DNA. No translation available.
CH236947 Genomic DNA. Translation: EAL24409.1.
CH471070 Genomic DNA. Translation: EAW83341.1.
BC023652 mRNA. Translation: AAH23652.1.
CCDSiCCDS47681.1. [O43913-2]
CCDS5734.1. [O43913-1]
RefSeqiNP_002544.1. NM_002553.3. [O43913-1]
NP_859531.1. NM_181747.3. [O43913-2]
UniGeneiHs.432948.

Genome annotation databases

EnsembliENST00000297431; ENSP00000297431; ENSG00000164815. [O43913-1]
ENST00000447452; ENSP00000395747; ENSG00000164815. [O43913-2]
GeneIDi5001.
KEGGihsa:5001.
UCSCiuc003vcb.3. human. [O43913-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiORC5_HUMAN
AccessioniPrimary (citable) accession number: O43913
Secondary accession number(s): A4D0P8, O60590, O95268
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: June 7, 2017
This is version 143 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families