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O43868 (S28A2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 124. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sodium/nucleoside cotransporter 2
Alternative name(s):
Concentrative nucleoside transporter 2
Short name=CNT 2
Short name=hCNT2
Na(+)/nucleoside cotransporter 2
Sodium-coupled nucleoside transporter 2
Sodium/purine nucleoside co-transporter
Short name=SPNT
Solute carrier family 28 member 2
Gene names
Name:SLC28A2
Synonyms:CNT2
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length658 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.

Enzyme regulation

Inhibited by formycin B.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.

Sequence similarities

Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 658658Sodium/nucleoside cotransporter 2
PRO_0000070450

Regions

Transmembrane80 – 10021Helical; Potential
Transmembrane104 – 12320Helical; Potential
Transmembrane148 – 16619Helical; Potential
Transmembrane172 – 19221Helical; Potential
Transmembrane200 – 22021Helical; Potential
Transmembrane233 – 25321Helical; Potential
Transmembrane260 – 28021Helical; Potential
Transmembrane295 – 31420Helical; Potential
Transmembrane336 – 35520Helical; Potential
Transmembrane362 – 38120Helical; Potential
Transmembrane423 – 44321Helical; Potential
Transmembrane454 – 47421Helical; Potential
Transmembrane529 – 54921Helical; Potential
Transmembrane567 – 58721Helical; Potential

Natural variations

Natural variant121L → P. Ref.7
VAR_036817
Natural variant221P → L. Ref.6 Ref.7
Corresponds to variant rs11854484 [ dbSNP | Ensembl ].
VAR_024639
Natural variant751S → R. Ref.2 Ref.6 Ref.7
Corresponds to variant rs1060896 [ dbSNP | Ensembl ].
VAR_024640
Natural variant1421R → H. Ref.7
Corresponds to variant rs115740452 [ dbSNP | Ensembl ].
VAR_036818
Natural variant1631L → W. Ref.6 Ref.7
Corresponds to variant rs2271437 [ dbSNP | Ensembl ].
VAR_022001
Natural variant1721E → D. Ref.7
Corresponds to variant rs113624548 [ dbSNP | Ensembl ].
VAR_036819
Natural variant2451S → T. Ref.6
Corresponds to variant rs10519020 [ dbSNP | Ensembl ].
VAR_024641
Natural variant3551F → S. Ref.6
Corresponds to variant rs17215633 [ dbSNP | Ensembl ].
VAR_024642
Natural variant3851E → K. Ref.7
VAR_036820
Natural variant4621L → F. Ref.6
Corresponds to variant rs17222057 [ dbSNP | Ensembl ].
VAR_024643
Natural variant5091G → E.
Corresponds to variant rs9635306 [ dbSNP | Ensembl ].
VAR_028724
Natural variant6121M → T. Ref.7
VAR_036821

Sequences

Sequence LengthMass (Da)Tools
O43868 [UniParc].

Last modified October 17, 2006. Version 2.
Checksum: 273545EE75E0D663

FASTA65871,926
        10         20         30         40         50         60 
MEKASGRQSI ALSTVETGTV NPGLELMEKE VEPEGSKRTD AQGHSLGDGL GPSTYQRRSR 

        70         80         90        100        110        120 
WPFSKARSFC KTHASLFKKI LLGLLCLAYA AYLLAACILN FQRALALFVI TCLVIFVLVH 

       130        140        150        160        170        180 
SFLKKLLGKK LTRCLKPFEN SRLRLWTKWV FAGVSLVGLI LWLALDTAQR PEQLIPFAGI 

       190        200        210        220        230        240 
CMFILILFAC SKHHSAVSWR TVFSGLGLQF VFGILVIRTD LGYTVFQWLG EQVQIFLNYT 

       250        260        270        280        290        300 
VAGSSFVFGD TLVKDVFAFQ ALPIIIFFGC VVSILYYLGL VQWVVQKVAW FLQITMGTTA 

       310        320        330        340        350        360 
TETLAVAGNI FVGMTEAPLL IRPYLGDMTL SEIHAVMTGG FATISGTVLG AFIAFGVDAS 

       370        380        390        400        410        420 
SLISASVMAA PCALASSKLA YPEVEESKFK SEEGVKLPRG KERNVLEAAS NGAVDAIGLA 

       430        440        450        460        470        480 
TNVAANLIAF LAVLAFINAA LSWLGELVDI QGLTFQVICS YLLRPMVFMM GVEWTDCPMV 

       490        500        510        520        530        540 
AEMVGIKFFI NEFVAYQQLS QYKNKRLSGM EEWIEGEKQW ISVRAEIITT FSLCGFANLS 

       550        560        570        580        590        600 
SIGITLGGLT SIVPHRKSDL SKVVVRALFT GACVSLISAC MAGILYVPRG AEADCVSFPN 

       610        620        630        640        650 
TSFTNRTYET YMCCRGLFQS TSLNGTNPPS FSGPWEDKEF SAMALTNCCG FYNNTVCA 

« Hide

References

« Hide 'large scale' references
[1]"Na+-dependent purine nucleoside transporter from human kidney: cloning and functional characterization."
Wang J., Su S.-F., Dresser M.J., Schaner M.E., Washington C.B., Giacomini K.M.
Am. J. Physiol. 273:F1058-F1065(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[2]"Molecular cloning, functional expression and chromosomal localization of a cDNA encoding a human Na+/nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridine."
Ritzel M.W.L., Yao S.Y.M., Ng A.M.L., Mackey J.R., Cass C.E., Young J.D.
Mol. Membr. Biol. 15:203-211(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-75.
Tissue: Small intestine.
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Small intestine.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Colon.
[5]"Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2."
Loewen S.K., Ng A.M.L., Yao S.Y.M., Cass C.E., Baldwin S.A., Young J.D.
J. Biol. Chem. 274:24475-24484(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS.
[6]"Genetic analysis and functional characterization of polymorphisms in the human concentrative nucleoside transporter, CNT2."
Owen R.P., Gray J.H., Taylor T.R., Carlson E.J., Huang C.C., Kawamoto M., Johns S.J., Stryke D., Ferrin T.E., Giacomini K.M.
Pharmacogenet. Genomics 15:83-90(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS LEU-22; ARG-75; TRP-163; THR-245; SER-355 AND PHE-462.
[7]"Identification and functional analysis of variants in the human concentrative nucleoside transporter 2, hCNT2 (SLC28A2) in Chinese, Malays and Indians."
Li L., Tan C.M.F., Koo S.H., Chong K.T., Lee E.J.D.
Pharmacogenet. Genomics 17:783-786(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: VARIANTS PRO-12; LEU-22; ARG-75; HIS-142; TRP-163; ASP-172; LYS-385 AND THR-612.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U84392 mRNA. Translation: AAC51930.1.
AF036109 mRNA. Translation: AAB88539.1.
AK291974 mRNA. Translation: BAF84663.1.
BC093737 mRNA. Translation: AAH93737.1.
RefSeqNP_004203.2. NM_004212.3.
UniGeneHs.367833.

3D structure databases

ProteinModelPortalO43868.
SMRO43868. Positions 179-585.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9606.ENSP00000315006.

Chemistry

BindingDBO43868.
ChEMBLCHEMBL5780.

Protein family/group databases

TCDB2.A.41.2.4. the concentrative nucleoside transporter (cnt) family.

PTM databases

PhosphoSiteO43868.

Proteomic databases

PaxDbO43868.
PRIDEO43868.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000347644; ENSP00000315006; ENSG00000137860.
GeneID9153.
KEGGhsa:9153.
UCSCuc001zva.2. human.

Organism-specific databases

CTD9153.
GeneCardsGC15P045544.
HGNCHGNC:11002. SLC28A2.
HPAHPA046068.
MIM606208. gene.
neXtProtNX_O43868.
PharmGKBPA386.
GenAtlasSearch...

Phylogenomic databases

eggNOGCOG1972.
HOGENOMHOG000267658.
HOVERGENHBG054073.
InParanoidO43868.
KOK11536.
OMAYNNTVCA.
OrthoDBEOG7RV9FR.
PhylomeDBO43868.
TreeFamTF314131.

Enzyme and pathway databases

ReactomeREACT_15518. Transmembrane transport of small molecules.

Gene expression databases

ArrayExpressO43868.
BgeeO43868.
CleanExHS_SLC28A2.
GenevestigatorO43868.

Family and domain databases

InterProIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR011657. Nucleos_tra2_C.
IPR011642. Nucleoside_recog_Gate.
IPR002668. Nuclsd_transpt2.
[Graphical view]
PANTHERPTHR10590. PTHR10590. 1 hit.
PfamPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsTIGR00804. nupC. 1 hit.
ProtoNetSearch...

Other

GeneWikiConcentrative_nucleoside_transporter_2.
GenomeRNAi9153.
NextBio34339.
PROO43868.
SOURCESearch...

Entry information

Entry nameS28A2_HUMAN
AccessionPrimary (citable) accession number: O43868
Secondary accession number(s): A8K7F9, O43239, Q52LZ0
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 17, 2006
Last modified: April 16, 2014
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 15

Human chromosome 15: entries, gene names and cross-references to MIM