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Protein

Sodium/nucleoside cotransporter 2

Gene

SLC28A2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.

Enzyme regulationi

Inhibited by formycin B.

GO - Molecular functioni

GO - Biological processi

  • nucleobase-containing compound metabolic process Source: ProtInc
  • purine nucleoside transmembrane transport Source: ProtInc
  • transmembrane transport Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

ReactomeiREACT_1206. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Protein family/group databases

TCDBi2.A.41.2.4. the concentrative nucleoside transporter (cnt) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Sodium/nucleoside cotransporter 2
Alternative name(s):
Concentrative nucleoside transporter 2
Short name:
CNT 2
Short name:
hCNT2
Na(+)/nucleoside cotransporter 2
Sodium-coupled nucleoside transporter 2
Sodium/purine nucleoside co-transporter
Short name:
SPNT
Solute carrier family 28 member 2
Gene namesi
Name:SLC28A2
Synonyms:CNT2
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 15

Organism-specific databases

HGNCiHGNC:11002. SLC28A2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei80 – 10021HelicalSequence AnalysisAdd
BLAST
Transmembranei104 – 12320HelicalSequence AnalysisAdd
BLAST
Transmembranei148 – 16619HelicalSequence AnalysisAdd
BLAST
Transmembranei172 – 19221HelicalSequence AnalysisAdd
BLAST
Transmembranei200 – 22021HelicalSequence AnalysisAdd
BLAST
Transmembranei233 – 25321HelicalSequence AnalysisAdd
BLAST
Transmembranei260 – 28021HelicalSequence AnalysisAdd
BLAST
Transmembranei295 – 31420HelicalSequence AnalysisAdd
BLAST
Transmembranei336 – 35520HelicalSequence AnalysisAdd
BLAST
Transmembranei362 – 38120HelicalSequence AnalysisAdd
BLAST
Transmembranei423 – 44321HelicalSequence AnalysisAdd
BLAST
Transmembranei454 – 47421HelicalSequence AnalysisAdd
BLAST
Transmembranei529 – 54921HelicalSequence AnalysisAdd
BLAST
Transmembranei567 – 58721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  • integral component of plasma membrane Source: ProtInc
  • membrane Source: ProtInc
  • plasma membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA386.

Chemistry

DrugBankiDB01033. Mercaptopurine.

Polymorphism and mutation databases

BioMutaiSLC28A2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 658658Sodium/nucleoside cotransporter 2PRO_0000070450Add
BLAST

Proteomic databases

PaxDbiO43868.
PRIDEiO43868.

PTM databases

PhosphoSiteiO43868.

Expressioni

Tissue specificityi

Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.

Gene expression databases

BgeeiO43868.
CleanExiHS_SLC28A2.
ExpressionAtlasiO43868. baseline and differential.
GenevisibleiO43868. HS.

Organism-specific databases

HPAiHPA046068.
HPA055623.

Interactioni

Protein-protein interaction databases

STRINGi9606.ENSP00000315006.

Structurei

3D structure databases

ProteinModelPortaliO43868.
SMRiO43868. Positions 179-585.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1972.
GeneTreeiENSGT00390000016025.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiO43868.
KOiK11536.
OMAiYNNTVCA.
OrthoDBiEOG7RV9FR.
PhylomeDBiO43868.
TreeFamiTF314131.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030212. CNT1/CNT2.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF11. PTHR10590:SF11. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.

Sequencei

Sequence statusi: Complete.

O43868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKASGRQSI ALSTVETGTV NPGLELMEKE VEPEGSKRTD AQGHSLGDGL
60 70 80 90 100
GPSTYQRRSR WPFSKARSFC KTHASLFKKI LLGLLCLAYA AYLLAACILN
110 120 130 140 150
FQRALALFVI TCLVIFVLVH SFLKKLLGKK LTRCLKPFEN SRLRLWTKWV
160 170 180 190 200
FAGVSLVGLI LWLALDTAQR PEQLIPFAGI CMFILILFAC SKHHSAVSWR
210 220 230 240 250
TVFSGLGLQF VFGILVIRTD LGYTVFQWLG EQVQIFLNYT VAGSSFVFGD
260 270 280 290 300
TLVKDVFAFQ ALPIIIFFGC VVSILYYLGL VQWVVQKVAW FLQITMGTTA
310 320 330 340 350
TETLAVAGNI FVGMTEAPLL IRPYLGDMTL SEIHAVMTGG FATISGTVLG
360 370 380 390 400
AFIAFGVDAS SLISASVMAA PCALASSKLA YPEVEESKFK SEEGVKLPRG
410 420 430 440 450
KERNVLEAAS NGAVDAIGLA TNVAANLIAF LAVLAFINAA LSWLGELVDI
460 470 480 490 500
QGLTFQVICS YLLRPMVFMM GVEWTDCPMV AEMVGIKFFI NEFVAYQQLS
510 520 530 540 550
QYKNKRLSGM EEWIEGEKQW ISVRAEIITT FSLCGFANLS SIGITLGGLT
560 570 580 590 600
SIVPHRKSDL SKVVVRALFT GACVSLISAC MAGILYVPRG AEADCVSFPN
610 620 630 640 650
TSFTNRTYET YMCCRGLFQS TSLNGTNPPS FSGPWEDKEF SAMALTNCCG

FYNNTVCA
Length:658
Mass (Da):71,926
Last modified:October 17, 2006 - v2
Checksum:i273545EE75E0D663
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti12 – 121L → P.1 Publication
VAR_036817
Natural varianti22 – 221P → L.2 Publications
Corresponds to variant rs11854484 [ dbSNP | Ensembl ].
VAR_024639
Natural varianti75 – 751S → R.3 Publications
Corresponds to variant rs1060896 [ dbSNP | Ensembl ].
VAR_024640
Natural varianti142 – 1421R → H.1 Publication
Corresponds to variant rs115740452 [ dbSNP | Ensembl ].
VAR_036818
Natural varianti163 – 1631L → W.2 Publications
Corresponds to variant rs2271437 [ dbSNP | Ensembl ].
VAR_022001
Natural varianti172 – 1721E → D.1 Publication
Corresponds to variant rs113624548 [ dbSNP | Ensembl ].
VAR_036819
Natural varianti245 – 2451S → T.1 Publication
Corresponds to variant rs10519020 [ dbSNP | Ensembl ].
VAR_024641
Natural varianti355 – 3551F → S.1 Publication
Corresponds to variant rs17215633 [ dbSNP | Ensembl ].
VAR_024642
Natural varianti385 – 3851E → K.1 Publication
VAR_036820
Natural varianti462 – 4621L → F.1 Publication
Corresponds to variant rs17222057 [ dbSNP | Ensembl ].
VAR_024643
Natural varianti509 – 5091G → E.
Corresponds to variant rs9635306 [ dbSNP | Ensembl ].
VAR_028724
Natural varianti612 – 6121M → T.1 Publication
VAR_036821

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84392 mRNA. Translation: AAC51930.1.
AF036109 mRNA. Translation: AAB88539.1.
AK291974 mRNA. Translation: BAF84663.1.
BC093737 mRNA. Translation: AAH93737.1.
CCDSiCCDS10121.1.
RefSeqiNP_004203.2. NM_004212.3.
UniGeneiHs.367833.

Genome annotation databases

EnsembliENST00000347644; ENSP00000315006; ENSG00000137860.
GeneIDi9153.
KEGGihsa:9153.
UCSCiuc001zva.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U84392 mRNA. Translation: AAC51930.1.
AF036109 mRNA. Translation: AAB88539.1.
AK291974 mRNA. Translation: BAF84663.1.
BC093737 mRNA. Translation: AAH93737.1.
CCDSiCCDS10121.1.
RefSeqiNP_004203.2. NM_004212.3.
UniGeneiHs.367833.

3D structure databases

ProteinModelPortaliO43868.
SMRiO43868. Positions 179-585.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000315006.

Chemistry

BindingDBiO43868.
ChEMBLiCHEMBL5780.
DrugBankiDB01033. Mercaptopurine.

Protein family/group databases

TCDBi2.A.41.2.4. the concentrative nucleoside transporter (cnt) family.

PTM databases

PhosphoSiteiO43868.

Polymorphism and mutation databases

BioMutaiSLC28A2.

Proteomic databases

PaxDbiO43868.
PRIDEiO43868.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000347644; ENSP00000315006; ENSG00000137860.
GeneIDi9153.
KEGGihsa:9153.
UCSCiuc001zva.2. human.

Organism-specific databases

CTDi9153.
GeneCardsiGC15P045544.
HGNCiHGNC:11002. SLC28A2.
HPAiHPA046068.
HPA055623.
MIMi606208. gene.
neXtProtiNX_O43868.
PharmGKBiPA386.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiCOG1972.
GeneTreeiENSGT00390000016025.
HOGENOMiHOG000267658.
HOVERGENiHBG054073.
InParanoidiO43868.
KOiK11536.
OMAiYNNTVCA.
OrthoDBiEOG7RV9FR.
PhylomeDBiO43868.
TreeFamiTF314131.

Enzyme and pathway databases

ReactomeiREACT_1206. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

Miscellaneous databases

GeneWikiiConcentrative_nucleoside_transporter_2.
GenomeRNAii9153.
NextBioi34339.
PROiO43868.
SOURCEiSearch...

Gene expression databases

BgeeiO43868.
CleanExiHS_SLC28A2.
ExpressionAtlasiO43868. baseline and differential.
GenevisibleiO43868. HS.

Family and domain databases

InterProiIPR008276. C_nuclsd_transpt.
IPR018270. C_nuclsd_transpt_met_bac.
IPR030212. CNT1/CNT2.
IPR011657. CNT_C_dom.
IPR002668. CNT_N_dom.
IPR011642. Nucleoside_recog_Gate.
[Graphical view]
PANTHERiPTHR10590. PTHR10590. 1 hit.
PTHR10590:SF11. PTHR10590:SF11. 1 hit.
PfamiPF07670. Gate. 1 hit.
PF07662. Nucleos_tra2_C. 1 hit.
PF01773. Nucleos_tra2_N. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00804. nupC. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Na+-dependent purine nucleoside transporter from human kidney: cloning and functional characterization."
    Wang J., Su S.-F., Dresser M.J., Schaner M.E., Washington C.B., Giacomini K.M.
    Am. J. Physiol. 273:F1058-F1065(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Molecular cloning, functional expression and chromosomal localization of a cDNA encoding a human Na+/nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridine."
    Ritzel M.W.L., Yao S.Y.M., Ng A.M.L., Mackey J.R., Cass C.E., Young J.D.
    Mol. Membr. Biol. 15:203-211(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-75.
    Tissue: Small intestine.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Small intestine.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Colon.
  5. "Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2."
    Loewen S.K., Ng A.M.L., Yao S.Y.M., Cass C.E., Baldwin S.A., Young J.D.
    J. Biol. Chem. 274:24475-24484(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS.
  6. "Genetic analysis and functional characterization of polymorphisms in the human concentrative nucleoside transporter, CNT2."
    Owen R.P., Gray J.H., Taylor T.R., Carlson E.J., Huang C.C., Kawamoto M., Johns S.J., Stryke D., Ferrin T.E., Giacomini K.M.
    Pharmacogenet. Genomics 15:83-90(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS LEU-22; ARG-75; TRP-163; THR-245; SER-355 AND PHE-462.
  7. "Identification and functional analysis of variants in the human concentrative nucleoside transporter 2, hCNT2 (SLC28A2) in Chinese, Malays and Indians."
    Li L., Tan C.M.F., Koo S.H., Chong K.T., Lee E.J.D.
    Pharmacogenet. Genomics 17:783-786(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS PRO-12; LEU-22; ARG-75; HIS-142; TRP-163; ASP-172; LYS-385 AND THR-612.

Entry informationi

Entry nameiS28A2_HUMAN
AccessioniPrimary (citable) accession number: O43868
Secondary accession number(s): A8K7F9, O43239, Q52LZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: October 17, 2006
Last modified: June 24, 2015
This is version 135 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 15
    Human chromosome 15: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.