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O43868

- S28A2_HUMAN

UniProt

O43868 - S28A2_HUMAN

Protein

Sodium/nucleoside cotransporter 2

Gene

SLC28A2

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 2 (17 Oct 2006)
      Previous versions | rss
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    Functioni

    Sodium-dependent and purine-selective transporter. Exhibits the transport characteristics of the nucleoside transport system cif or N1 subtype (N1/cif) (selective for purine nucleosides and uridine). Plays a critical role in specific uptake and salvage of purine nucleosides in kidney and other tissues.

    Enzyme regulationi

    Inhibited by formycin B.

    GO - Molecular functioni

    1. nucleoside:sodium symporter activity Source: InterPro
    2. nucleoside binding Source: InterPro
    3. purine nucleoside transmembrane transporter activity Source: ProtInc

    GO - Biological processi

    1. nucleobase-containing compound metabolic process Source: ProtInc
    2. purine nucleoside transmembrane transport Source: ProtInc
    3. transmembrane transport Source: Reactome

    Keywords - Biological processi

    Transport

    Enzyme and pathway databases

    ReactomeiREACT_1206. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

    Protein family/group databases

    TCDBi2.A.41.2.4. the concentrative nucleoside transporter (cnt) family.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sodium/nucleoside cotransporter 2
    Alternative name(s):
    Concentrative nucleoside transporter 2
    Short name:
    CNT 2
    Short name:
    hCNT2
    Na(+)/nucleoside cotransporter 2
    Sodium-coupled nucleoside transporter 2
    Sodium/purine nucleoside co-transporter
    Short name:
    SPNT
    Solute carrier family 28 member 2
    Gene namesi
    Name:SLC28A2
    Synonyms:CNT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 15

    Organism-specific databases

    HGNCiHGNC:11002. SLC28A2.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of plasma membrane Source: ProtInc
    2. membrane Source: ProtInc
    3. plasma membrane Source: Reactome

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA386.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 658658Sodium/nucleoside cotransporter 2PRO_0000070450Add
    BLAST

    Proteomic databases

    PaxDbiO43868.
    PRIDEiO43868.

    PTM databases

    PhosphoSiteiO43868.

    Expressioni

    Tissue specificityi

    Expressed in heart and skeletal muscle followed by liver, kidney, intestine, pancreas, placenta and brain. Weak expression in lung.

    Gene expression databases

    ArrayExpressiO43868.
    BgeeiO43868.
    CleanExiHS_SLC28A2.
    GenevestigatoriO43868.

    Organism-specific databases

    HPAiHPA046068.

    Interactioni

    Protein-protein interaction databases

    STRINGi9606.ENSP00000315006.

    Structurei

    3D structure databases

    ProteinModelPortaliO43868.
    SMRiO43868. Positions 179-585.
    ModBaseiSearch...
    MobiDBiSearch...

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei80 – 10021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei104 – 12320HelicalSequence AnalysisAdd
    BLAST
    Transmembranei148 – 16619HelicalSequence AnalysisAdd
    BLAST
    Transmembranei172 – 19221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei200 – 22021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei233 – 25321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei260 – 28021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei295 – 31420HelicalSequence AnalysisAdd
    BLAST
    Transmembranei336 – 35520HelicalSequence AnalysisAdd
    BLAST
    Transmembranei362 – 38120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei423 – 44321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei454 – 47421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei529 – 54921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei567 – 58721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG1972.
    HOGENOMiHOG000267658.
    HOVERGENiHBG054073.
    InParanoidiO43868.
    KOiK11536.
    OMAiSRLIMTY.
    OrthoDBiEOG7RV9FR.
    PhylomeDBiO43868.
    TreeFamiTF314131.

    Family and domain databases

    InterProiIPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view]
    PANTHERiPTHR10590. PTHR10590. 1 hit.
    PfamiPF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00804. nupC. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O43868-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEKASGRQSI ALSTVETGTV NPGLELMEKE VEPEGSKRTD AQGHSLGDGL    50
    GPSTYQRRSR WPFSKARSFC KTHASLFKKI LLGLLCLAYA AYLLAACILN 100
    FQRALALFVI TCLVIFVLVH SFLKKLLGKK LTRCLKPFEN SRLRLWTKWV 150
    FAGVSLVGLI LWLALDTAQR PEQLIPFAGI CMFILILFAC SKHHSAVSWR 200
    TVFSGLGLQF VFGILVIRTD LGYTVFQWLG EQVQIFLNYT VAGSSFVFGD 250
    TLVKDVFAFQ ALPIIIFFGC VVSILYYLGL VQWVVQKVAW FLQITMGTTA 300
    TETLAVAGNI FVGMTEAPLL IRPYLGDMTL SEIHAVMTGG FATISGTVLG 350
    AFIAFGVDAS SLISASVMAA PCALASSKLA YPEVEESKFK SEEGVKLPRG 400
    KERNVLEAAS NGAVDAIGLA TNVAANLIAF LAVLAFINAA LSWLGELVDI 450
    QGLTFQVICS YLLRPMVFMM GVEWTDCPMV AEMVGIKFFI NEFVAYQQLS 500
    QYKNKRLSGM EEWIEGEKQW ISVRAEIITT FSLCGFANLS SIGITLGGLT 550
    SIVPHRKSDL SKVVVRALFT GACVSLISAC MAGILYVPRG AEADCVSFPN 600
    TSFTNRTYET YMCCRGLFQS TSLNGTNPPS FSGPWEDKEF SAMALTNCCG 650
    FYNNTVCA 658
    Length:658
    Mass (Da):71,926
    Last modified:October 17, 2006 - v2
    Checksum:i273545EE75E0D663
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti12 – 121L → P.1 Publication
    VAR_036817
    Natural varianti22 – 221P → L.2 Publications
    Corresponds to variant rs11854484 [ dbSNP | Ensembl ].
    VAR_024639
    Natural varianti75 – 751S → R.3 Publications
    Corresponds to variant rs1060896 [ dbSNP | Ensembl ].
    VAR_024640
    Natural varianti142 – 1421R → H.1 Publication
    Corresponds to variant rs115740452 [ dbSNP | Ensembl ].
    VAR_036818
    Natural varianti163 – 1631L → W.2 Publications
    Corresponds to variant rs2271437 [ dbSNP | Ensembl ].
    VAR_022001
    Natural varianti172 – 1721E → D.1 Publication
    Corresponds to variant rs113624548 [ dbSNP | Ensembl ].
    VAR_036819
    Natural varianti245 – 2451S → T.1 Publication
    Corresponds to variant rs10519020 [ dbSNP | Ensembl ].
    VAR_024641
    Natural varianti355 – 3551F → S.1 Publication
    Corresponds to variant rs17215633 [ dbSNP | Ensembl ].
    VAR_024642
    Natural varianti385 – 3851E → K.1 Publication
    VAR_036820
    Natural varianti462 – 4621L → F.1 Publication
    Corresponds to variant rs17222057 [ dbSNP | Ensembl ].
    VAR_024643
    Natural varianti509 – 5091G → E.
    Corresponds to variant rs9635306 [ dbSNP | Ensembl ].
    VAR_028724
    Natural varianti612 – 6121M → T.1 Publication
    VAR_036821

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U84392 mRNA. Translation: AAC51930.1.
    AF036109 mRNA. Translation: AAB88539.1.
    AK291974 mRNA. Translation: BAF84663.1.
    BC093737 mRNA. Translation: AAH93737.1.
    CCDSiCCDS10121.1.
    RefSeqiNP_004203.2. NM_004212.3.
    UniGeneiHs.367833.

    Genome annotation databases

    EnsembliENST00000347644; ENSP00000315006; ENSG00000137860.
    GeneIDi9153.
    KEGGihsa:9153.
    UCSCiuc001zva.2. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U84392 mRNA. Translation: AAC51930.1 .
    AF036109 mRNA. Translation: AAB88539.1 .
    AK291974 mRNA. Translation: BAF84663.1 .
    BC093737 mRNA. Translation: AAH93737.1 .
    CCDSi CCDS10121.1.
    RefSeqi NP_004203.2. NM_004212.3.
    UniGenei Hs.367833.

    3D structure databases

    ProteinModelPortali O43868.
    SMRi O43868. Positions 179-585.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9606.ENSP00000315006.

    Chemistry

    BindingDBi O43868.
    ChEMBLi CHEMBL5780.

    Protein family/group databases

    TCDBi 2.A.41.2.4. the concentrative nucleoside transporter (cnt) family.

    PTM databases

    PhosphoSitei O43868.

    Proteomic databases

    PaxDbi O43868.
    PRIDEi O43868.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000347644 ; ENSP00000315006 ; ENSG00000137860 .
    GeneIDi 9153.
    KEGGi hsa:9153.
    UCSCi uc001zva.2. human.

    Organism-specific databases

    CTDi 9153.
    GeneCardsi GC15P045544.
    HGNCi HGNC:11002. SLC28A2.
    HPAi HPA046068.
    MIMi 606208. gene.
    neXtProti NX_O43868.
    PharmGKBi PA386.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1972.
    HOGENOMi HOG000267658.
    HOVERGENi HBG054073.
    InParanoidi O43868.
    KOi K11536.
    OMAi SRLIMTY.
    OrthoDBi EOG7RV9FR.
    PhylomeDBi O43868.
    TreeFami TF314131.

    Enzyme and pathway databases

    Reactomei REACT_1206. Transport of nucleosides and free purine and pyrimidine bases across the plasma membrane.

    Miscellaneous databases

    GeneWikii Concentrative_nucleoside_transporter_2.
    GenomeRNAii 9153.
    NextBioi 34339.
    PROi O43868.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43868.
    Bgeei O43868.
    CleanExi HS_SLC28A2.
    Genevestigatori O43868.

    Family and domain databases

    InterProi IPR008276. C_nuclsd_transpt.
    IPR018270. C_nuclsd_transpt_met_bac.
    IPR011657. CNT_C_dom.
    IPR002668. CNT_N_dom.
    IPR011642. Nucleoside_recog_Gate.
    [Graphical view ]
    PANTHERi PTHR10590. PTHR10590. 1 hit.
    Pfami PF07670. Gate. 1 hit.
    PF07662. Nucleos_tra2_C. 1 hit.
    PF01773. Nucleos_tra2_N. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00804. nupC. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Na+-dependent purine nucleoside transporter from human kidney: cloning and functional characterization."
      Wang J., Su S.-F., Dresser M.J., Schaner M.E., Washington C.B., Giacomini K.M.
      Am. J. Physiol. 273:F1058-F1065(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Kidney.
    2. "Molecular cloning, functional expression and chromosomal localization of a cDNA encoding a human Na+/nucleoside cotransporter (hCNT2) selective for purine nucleosides and uridine."
      Ritzel M.W.L., Yao S.Y.M., Ng A.M.L., Mackey J.R., Cass C.E., Young J.D.
      Mol. Membr. Biol. 15:203-211(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT ARG-75.
      Tissue: Small intestine.
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Small intestine.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Colon.
    5. "Identification of amino acid residues responsible for the pyrimidine and purine nucleoside specificities of human concentrative Na(+) nucleoside cotransporters hCNT1 and hCNT2."
      Loewen S.K., Ng A.M.L., Yao S.Y.M., Cass C.E., Baldwin S.A., Young J.D.
      J. Biol. Chem. 274:24475-24484(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS.
    6. "Genetic analysis and functional characterization of polymorphisms in the human concentrative nucleoside transporter, CNT2."
      Owen R.P., Gray J.H., Taylor T.R., Carlson E.J., Huang C.C., Kawamoto M., Johns S.J., Stryke D., Ferrin T.E., Giacomini K.M.
      Pharmacogenet. Genomics 15:83-90(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS LEU-22; ARG-75; TRP-163; THR-245; SER-355 AND PHE-462.
    7. "Identification and functional analysis of variants in the human concentrative nucleoside transporter 2, hCNT2 (SLC28A2) in Chinese, Malays and Indians."
      Li L., Tan C.M.F., Koo S.H., Chong K.T., Lee E.J.D.
      Pharmacogenet. Genomics 17:783-786(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: VARIANTS PRO-12; LEU-22; ARG-75; HIS-142; TRP-163; ASP-172; LYS-385 AND THR-612.

    Entry informationi

    Entry nameiS28A2_HUMAN
    AccessioniPrimary (citable) accession number: O43868
    Secondary accession number(s): A8K7F9, O43239, Q52LZ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 1, 2000
    Last sequence update: October 17, 2006
    Last modified: October 1, 2014
    This is version 129 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 15
      Human chromosome 15: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3