O43866 (CD5L_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
CD5 antigen-like

Alternative name(s):
CT-2
IgM-associated peptide
SP-alpha

Gene names

Name:	CD5L
Synonyms:	API6
ORF Names:	UNQ203/PRO229

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	347 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May play a role in the regulation of the immune system. Seems to play a role as an inhibitor of apoptosis.
Subunit structure	Associated with IgM.
Subcellular location	Secreted.
Tissue specificity	Expressed in spleen, lymph node, thymus, bone marrow, and fetal liver, but not in non-lymphoid tissues. Ref.1
Sequence similarities	Contains 3 SRCR domains.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Secreted
Coding sequence diversity	Polymorphism
Domain	Repeat Signal
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW cellular defense response Traceable author statement. Source: ProtInc
Cellular component	extracellular space Traceable author statement. Source: ProtInc membrane Inferred from electronic annotation. Source: InterPro
Molecular function	scavenger receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Ref.8 Ref.10
	Chain	20 – 347	328	CD5 antigen-like		PRO_0000033225
Regions
	Domain	24 – 125	102	SRCR 1
	Domain	138 – 239	102	SRCR 2
	Domain	244 – 346	103	SRCR 3
Amino acid modifications
	Disulfide bond	33 ↔ 67		By similarity
	Disulfide bond	49 ↔ 114		By similarity
	Disulfide bond	62 ↔ 124		By similarity
	Disulfide bond	96 ↔ 106		By similarity
	Disulfide bond	163 ↔ 228		By similarity
	Disulfide bond	176 ↔ 238		By similarity
	Disulfide bond	208 ↔ 218		By similarity
	Disulfide bond	253 ↔ 287		By similarity
	Disulfide bond	269 ↔ 335		By similarity
	Disulfide bond	282 ↔ 345		By similarity
	Disulfide bond	315 ↔ 325		By similarity
Natural variations
	Natural variant	117	1	D → E. Corresponds to variant rs11537583 [ dbSNP | Ensembl ].		VAR_033728
Experimental info
	Sequence conflict	347	1	G → V in AAQ88858. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O43866 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 40A8BE08F9495D83	FASTA	347	38,088
	10 20 30 40 50 60 MALLFSLILA ICTRPGFLAS PSGVRLVGGL HRCEGRVEVE QKGQWGTVCD DGWDIKDVAV 70 80 90 100 110 120 LCRELGCGAA SGTPSGILYE PPAEKEQKVL IQSVSCTGTE DTLAQCEQEE VYDCSHDEDA 130 140 150 160 170 180 GASCENPESS FSPVPEGVRL ADGPGHCKGR VEVKHQNQWY TVCQTGWSLR AAKVVCRQLG 190 200 210 220 230 240 CGRAVLTQKR CNKHAYGRKP IWLSQMSCSG REATLQDCPS GPWGKNTCNH DEDTWVECED 250 260 270 280 290 300 PFDLRLVGGD NLCSGRLEVL HKGVWGSVCD DNWGEKEDQV VCKQLGCGKS LSPSFRDRKC 310 320 330 340 YGPGVGRIWL DNVRCSGEEQ SLEQCQHRFW GFHDCTHQED VAVICSG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning, mapping to human chromosome 1 q21-q23, and cell binding characteristics of Spalpha, a new member of the scavenger receptor cysteine-rich (SRCR) family of proteins." Gebe J.A., Kiener P.A., Ring H.Z., Li X., Francke U., Aruffo A. J. Biol. Chem. 272:6151-6158(1997) [PubMed: 9045627] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF N-TERMINUS, TISSUE SPECIFICITY. Tissue: Spleen.
[2]	"Human CT-2 cDNA." Miyazaki T., Yusa S. Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment." Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E. , Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M. Genome Res. 13:2265-2270(2003) [PubMed: 12975309] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Spleen.
[5]	"The DNA sequence and biological annotation of human chromosome 1." Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K. , Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R. Nature 441:315-321(2006) [PubMed: 16710414] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Pancreas and Spleen.
[8]	"Two-dimensional polyacrylamide gel electrophoresis analysis of cryoglobulins and identification of an IgM-associated peptide." Tissot J.-D., Schifferli J.A., Hochstrasser D.F., Pasquali C., Spertini F., Clement F., Frutiger S., Paquet N., Hughes G.J., Schneider P. J. Immunol. Methods 173:63-75(1994) [PubMed: 8034987] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-31, BINDING TO IGM.
[9]	"IgM are associated to Spalpha (CD5 antigen-like)." Tissot J.D., Sanchez J.-C., Vuadens F., Scherl A., Schifferli J.A., Hochstrasser D.F., Schneider P., Duchosal M.A. Electrophoresis 23:1203-1206(2002) [PubMed: 11981870] [Abstract] Cited for: PARTIAL PROTEIN SEQUENCE, MASS SPECTROMETRY.
[10]	"Signal peptide prediction based on analysis of experimentally verified cleavage sites." Zhang Z., Henzel W.J. Protein Sci. 13:2819-2824(2004) [PubMed: 15340161] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-34.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U82812 mRNA. Translation: AAB91989.1. AF011429 mRNA. Translation: AAD01446.1. AY358494 mRNA. Translation: AAQ88858.1. AK292040 mRNA. Translation: BAF84729.1. AL139409 Genomic DNA. Translation: CAC19458.1. CH471121 Genomic DNA. Translation: EAW52859.1. BC033586 mRNA. Translation: AAH33586.1.
IPI	IPI00025204.
RefSeq	NP_005885.1. NM_005894.2.
UniGene	Hs.134035.
3D structure databases
ProteinModelPortal	O43866.
SMR	O43866. Positions 20-128, 136-346.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43866. 6 interactions.
MINT	MINT-6769012.
STRING	O43866.
Proteomic databases
PeptideAtlas	O43866.
PRIDE	O43866.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000368174; ENSP00000357156; ENSG00000073754.
GeneID	922.
KEGG	hsa:922.
UCSC	uc001frk.2. human.
Organism-specific databases
CTD	922.
GeneCards	GC01M157800.
H-InvDB	HIX0001186.
HGNC	HGNC:1690. CD5L.
MIM	602592. gene.
neXtProt	NX_O43866.
PharmGKB	PA26229.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG04032.
GeneTree	ENSGT00590000082847.
HOGENOM	HBG717356.
HOVERGEN	HBG031373.
InParanoid	O43866.
OMA	KEDQVVC.
OrthoDB	EOG4868CM.
PhylomeDB	O43866.
Gene expression databases
ArrayExpress	O43866.
Bgee	O43866.
CleanEx	HS_CD5L.
Genevestigator	O43866.
GermOnline	ENSG00000073754. Homo sapiens.
Family and domain databases
InterPro	IPR001190. Srcr_rcpt. IPR017448. Srcr_rcpt-rel. [Graphical view]
Pfam	PF00530. SRCR. 3 hits. [Graphical view]
PRINTS	PR00258. SPERACTRCPTR.
SMART	SM00202. SR. 3 hits. [Graphical view]
SUPFAM	SSF56487. Srcr_receptor. 3 hits.
PROSITE	PS00420. SRCR_1. 2 hits. PS50287. SRCR_2. 3 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	3814.
SOURCE	Search...

Entry information

Entry name

CD5L_HUMAN

Accession

Primary (citable) accession number: O43866
Secondary accession number(s): A8K7M5, Q6UX63

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 16, 2002
Last sequence update:	June 1, 1998
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.