O43854 (EDIL3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
EGF-like repeat and discoidin I-like domain-containing protein 3

Alternative name(s):
Developmentally-regulated endothelial cell locus 1 protein
Integrin-binding protein DEL1

Gene names

Name:	EDIL3
Synonyms:	DEL1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	480 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development.
Subcellular location	Secreted.
Sequence similarities	Contains 3 EGF-like domains. Contains 2 F5/8 type C domains.

Ontologies

Keywords
Biological process	Cell adhesion
Cellular component	Secreted
Coding sequence diversity	Alternative splicing
Domain	EGF-like domain Repeat Signal
Molecular function	Developmental protein
PTM	Disulfide bond
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell adhesion Inferred from electronic annotation. Source: UniProtKB-KW multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cell-substrate adhesion Inferred from electronic annotation. Source: Compara
Cellular_component	extracellular vesicular exosome Inferred from direct assay PubMed 21362503. Source: UniProtKB
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro integrin binding Traceable author statement Ref.1. Source: ProtInc
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43854-1)

Also known as: Long;

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43854-2)

Also known as: Short; Z20;

The sequence of this isoform differs from the canonical sequence as follows:
     66-66: A → G
     67-76: Missing.

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 16

16

Potential

Chain

17 – 480

464

EGF-like repeat and discoidin I-like domain-containing protein 3

PRO_0000007522

Regions

Domain

22 – 60

39

EGF-like 1

Domain

74 – 117

44

EGF-like 2

Domain

119 – 155

37

EGF-like 3

Domain

158 – 314

157

F5/8 type C 1

Domain

319 – 476

158

F5/8 type C 2

Motif

96 – 98

3

Cell attachment site Potential

Amino acid modifications

Disulfide bond

26 ↔ 37

By similarity

Disulfide bond

31 ↔ 48

By similarity

Disulfide bond

50 ↔ 59

By similarity

Disulfide bond

78 ↔ 89

By similarity

Disulfide bond

83 ↔ 105

By similarity

Disulfide bond

107 ↔ 116

By similarity

Disulfide bond

123 ↔ 134

By similarity

Disulfide bond

128 ↔ 143

By similarity

Disulfide bond

145 ↔ 154

By similarity

Disulfide bond

158 ↔ 314

By similarity

Disulfide bond

301 ↔ 305

By similarity

Disulfide bond

319 ↔ 476

By similarity

Natural variations

Alternative sequence

66

1

A → G in isoform 2.

VSP_050006

Alternative sequence

67 – 76

10

Missing in isoform 2.

VSP_050007

Secondary structure

1

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480

Helix Strand Turn

Details...

Helix

32 – 34

3

Beta strand

36 – 38

3

Beta strand

47 – 49

3

Turn

56 – 59

4

Turn

66 – 70

5

Beta strand

88 – 91

4

Beta strand

103 – 106

4

Beta strand

111 – 113

3

Turn

122 – 125

4

Beta strand

133 – 137

5

Beta strand

140 – 144

5

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 (Long) [UniParc]. Last modified June 1, 1998. Version 1. Checksum: F7171E23A309FD48	FASTA	480	53,765
	10 20 30 40 50 60 MKRSVAVWLL VGLSLGVPQF GKGDICDPNP CENGGICLPG LADGSFSCEC PDGFTDPNCS 70 80 90 100 110 120 SVVEVASDEE EPTSAGPCTP NPCHNGGTCE ISEAYRGDTF IGYVCKCPRG FNGIHCQHNI 130 140 150 160 170 180 NECEVEPCKN GGICTDLVAN YSCECPGEFM GRNCQYKCSG PLGIEGGIIS NQQITASSTH 190 200 210 220 230 240 RALFGLQKWY PYYARLNKKG LINAWTAAEN DRWPWIQINL QRKMRVTGVI TQGAKRIGSP 250 260 270 280 290 300 EYIKSYKIAY SNDGKTWAMY KVKGTNEDMV FRGNIDNNTP YANSFTPPIK AQYVRLYPQV 310 320 330 340 350 360 CRRHCTLRME LLGCELSGCS EPLGMKSGHI QDYQITASSI FRTLNMDMFT WEPRKARLDK 370 380 390 400 410 420 QGKVNAWTSG HNDQSQWLQV DLLVPTKVTG IITQGAKDFG HVQFVGSYKL AYSNDGEHWT 430 440 450 460 470 480 VYQDEKQRKD KVFQGNFDND THRKNVIDPP IYARHIRILP WSWYGRITLR SELLGCTEEE
	Isoform 2 (Short) (Z20) [UniParc]. Checksum: EFF139A13FE5337A	FASTA	470	52,748
	10 20 30 40 50 60 MKRSVAVWLL VGLSLGVPQF GKGDICDPNP CENGGICLPG LADGSFSCEC PDGFTDPNCS 70 80 90 100 110 120 SVVEVGPCTP NPCHNGGTCE ISEAYRGDTF IGYVCKCPRG FNGIHCQHNI NECEVEPCKN 130 140 150 160 170 180 GGICTDLVAN YSCECPGEFM GRNCQYKCSG PLGIEGGIIS NQQITASSTH RALFGLQKWY 190 200 210 220 230 240 PYYARLNKKG LINAWTAAEN DRWPWIQINL QRKMRVTGVI TQGAKRIGSP EYIKSYKIAY 250 260 270 280 290 300 SNDGKTWAMY KVKGTNEDMV FRGNIDNNTP YANSFTPPIK AQYVRLYPQV CRRHCTLRME 310 320 330 340 350 360 LLGCELSGCS EPLGMKSGHI QDYQITASSI FRTLNMDMFT WEPRKARLDK QGKVNAWTSG 370 380 390 400 410 420 HNDQSQWLQV DLLVPTKVTG IITQGAKDFG HVQFVGSYKL AYSNDGEHWT VYQDEKQRKD 430 440 450 460 470 KVFQGNFDND THRKNVIDPP IYARHIRILP WSWYGRITLR SELLGCTEEE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning and characterization of developmental endothelial locus-1: an embryonic endothelial cell protein that binds the alphavbeta3 integrin receptor." Hidai C., Zupancic T.J., Penta K., Mikhail A., Kawana M., Quertermous E.E., Aoka Y., Fukagawa M., Matsui Y., Platika D., Auerbach R., Hogan B.L.M., Snodgrass R., Quertermous T. Genes Dev. 12:21-33(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), CHARACTERIZATION. Tissue: Embryonic lung.
[2]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Trachea.
[3]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U70312 mRNA. Translation: AAC02648.1.
U70313 mRNA. Translation: AAC02649.1.
AK312858 mRNA. Translation: BAG35710.1.
CH471084 Genomic DNA. Translation: EAW95917.1.
CH471084 Genomic DNA. Translation: EAW95918.1.
BC053656 mRNA. Translation: AAH53656.1.

IPI

IPI00306046.
IPI00399105.

RefSeq

NP_005702.3. NM_005711.3.

UniGene

Hs.482730.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
4D90	X-ray	2.60	A/B	24-157	[»]

ProteinModelPortal

O43854.

ModBase

Search...

Protein-protein interaction databases

IntAct

O43854. 1 interaction.

STRING

9606.ENSP00000296591.

PTM databases

PhosphoSite

O43854.

Proteomic databases

PaxDb

O43854.

PRIDE

O43854.

Protocols and materials databases

DNASU

10085.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000296591; ENSP00000296591; ENSG00000164176.
ENST00000380138; ENSP00000369483; ENSG00000164176.

GeneID

10085.

KEGG

hsa:10085.

UCSC

uc003kio.1. human.
uc003kip.1. human.

Organism-specific databases

CTD

10085.

GeneCards

GC05M083272.

HGNC

HGNC:3173. EDIL3.

HPA

HPA020415.

MIM

606018. gene.

neXtProt

NX_O43854.

PharmGKB

PA27613.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG151278.

HOGENOM

HOG000236278.

HOVERGEN

HBG002385.

InParanoid

O43854.

OMA

EYMGRNC.

OrthoDB

EOG4SXNC7.

PhylomeDB

O43854.

Enzyme and pathway databases

Pathway_Interaction_DB

avb3_integrin_pathway. Integrins in angiogenesis.

Gene expression databases

Bgee

O43854.

CleanEx

HS_EDIL3.

Genevestigator

O43854.

GermOnline

ENSG00000164176. Homo sapiens.

Family and domain databases

InterPro

IPR000421. Coagulation_fac_5/8-C_type_dom.
IPR000742. EG-like_dom.
IPR001881. EGF-like_Ca-bd.
IPR013032. EGF-like_CS.
IPR000152. EGF-type_Asp/Asn_hydroxyl_site.
IPR018097. EGF_Ca-bd_CS.
IPR008979. Galactose-bd-like.
[Graphical view]

Pfam

PF00008. EGF. 2 hits.
PF00754. F5_F8_type_C. 2 hits.
[Graphical view]

SMART

SM00181. EGF. 2 hits.
SM00179. EGF_CA. 1 hit.
SM00231. FA58C. 2 hits.
[Graphical view]

SUPFAM

SSF49785. Gal_bind_like. 2 hits.

PROSITE

PS00010. ASX_HYDROXYL. 1 hit.
PS00022. EGF_1. 2 hits.
PS01186. EGF_2. 2 hits.
PS50026. EGF_3. 3 hits.
PS01187. EGF_CA. 1 hit.
PS01285. FA58C_1. 2 hits.
PS01286. FA58C_2. 2 hits.
PS50022. FA58C_3. 2 hits.
[Graphical view]

ProtoNet

Search...

Other

GenomeRNAi

10085.

NextBio

38137.

SOURCE

Search...

Entry information

Entry name

EDIL3_HUMAN

Accession

Primary (citable) accession number: O43854
Secondary accession number(s): B2R763, O43855, Q5D094

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 16, 2004
Last sequence update:	June 1, 1998
Last modified:	May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.