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Reviewed, UniProtKB/Swiss-Prot O43837 (IDH3B_HUMAN)

Last modified November 25, 2008. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
    EC=1.1.1.41
Alternative name(s):
    Isocitric dehydrogenase
    NAD(+)-specific ICDH
Gene names
Name: IDH3B
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level.

General annotation (Comments)

Catalytic activity

Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH.

Cofactor

Binds 1 magnesium or manganese ion per subunit By similarity.

Subunit structure

Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 By similarity.

Subcellular location

Mitochondrion.

Sequence similarities

Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform B (identifier: O43837-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform A (identifier: O43837-2)

The sequence of this isoform differs from the canonical sequence as follows:
     361-385: RDMGGYSTTTDFIKSVIGHLQTKGS → SDMGGYATCHDFTEAVIAALPHP

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3434Mitochondrion By similarity
Chain35 – 385351Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
PRO_0000014444

Sites

Metal binding2511Magnesium or manganese By similarity
Binding site1331Substrate By similarity
Binding site1641Substrate By similarity
Binding site2511Substrate By similarity
Site1711Critical for catalysis By similarity
Site2181Critical for catalysis By similarity

Natural variations

Alternative sequence361 – 38525RDMGG…QTKGS → SDMGGYATCHDFTEAVIAAL PHP in isoform A.
VSP_002462
Natural variant31A → V: dbSNP rs3178817.
VAR_022660

Experimental info

Sequence conflict127 – 1304SYDM → RTLV Ref.5
Sequence conflict3851S → SNL in AAD09340. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform B [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 7324E6CC30A68EE2

FASTA38542,184
        10         20         30         40         50         60 
MAALSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 

       130        140        150        160        170        180 
YKGELASYDM RLRRKLDLFA NVVHVKSLPG YMTRHNNLDL VIIREQTEGE YSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RAKSQRIAKF AFDYATKKGR GKVTAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL QTKGS 

« Hide

Isoform A [UniParc].

Checksum: 6B5A3D3F82CCDB34
Show »

38341,887

References

« Hide 'large scale' references
[1]"Mitochondrial NAD+-specific isocitrate dehydrogenase."
Ko H.-J., Kim Y.-O., Park H., Oh I., Yeo S., Hong S., Chae B., Kim S., Son B., Lee Y., Yeo H., Huh T.-L.
Submitted (FEB-1996) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), VARIANT VAL-3.
Tissue: Heart.
[2]Ko H.-J., Kim Y.-O., Huh T.-L.
Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A AND B), VARIANT VAL-3.
[3]"The DNA sequence and comparative analysis of human chromosome 20."
Deloukas P., Matthews L.H., Ashurst J.L., Burton J., Gilbert J.G.R., Jones M., Stavrides G., Almeida J.P., Babbage A.K., Bagguley C.L., Bailey J., Barlow K.F., Bates K.N., Beard L.M., Beare D.M., Beasley O.P., Bird C.P., Blakey S.E. expand/collapse author list , Bridgeman A.M., Brown A.J., Buck D., Burrill W.D., Butler A.P., Carder C., Carter N.P., Chapman J.C., Clamp M., Clark G., Clark L.N., Clark S.Y., Clee C.M., Clegg S., Cobley V.E., Collier R.E., Connor R.E., Corby N.R., Coulson A., Coville G.J., Deadman R., Dhami P.D., Dunn M., Ellington A.G., Frankland J.A., Fraser A., French L., Garner P., Grafham D.V., Griffiths C., Griffiths M.N.D., Gwilliam R., Hall R.E., Hammond S., Harley J.L., Heath P.D., Ho S., Holden J.L., Howden P.J., Huckle E., Hunt A.R., Hunt S.E., Jekosch K., Johnson C.M., Johnson D., Kay M.P., Kimberley A.M., King A., Knights A., Laird G.K., Lawlor S., Lehvaeslaiho M.H., Leversha M.A., Lloyd C., Lloyd D.M., Lovell J.D., Marsh V.L., Martin S.L., McConnachie L.J., McLay K., McMurray A.A., Milne S.A., Mistry D., Moore M.J.F., Mullikin J.C., Nickerson T., Oliver K., Parker A., Patel R., Pearce T.A.V., Peck A.I., Phillimore B.J.C.T., Prathalingam S.R., Plumb R.W., Ramsay H., Rice C.M., Ross M.T., Scott C.E., Sehra H.K., Shownkeen R., Sims S., Skuce C.D., Smith M.L., Soderlund C., Steward C.A., Sulston J.E., Swann R.M., Sycamore N., Taylor R., Tee L., Thomas D.W., Thorpe A., Tracey A., Tromans A.C., Vaudin M., Wall M., Wallis J.M., Whitehead S.L., Whittaker P., Willey D.L., Williams L., Williams S.A., Wilming L., Wray P.W., Hubbard T., Durbin R.M., Bentley D.R., Beck S., Rogers J.
Nature 414:865-871(2001) [PubMed: 11780052] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
Tissue: Lung.
[5]The German cDNA consortium
Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 127-385 (ISOFORM B).
Tissue: Uterus.
+Additional computationally mapped references.

Cross-references

Sequence databases

U49283 mRNA. Translation: AAB94295.1.
AF023265 mRNA. Translation: AAD09339.1.
AF023266 mRNA. Translation: AAD09340.1.
AL049712 Genomic DNA. Translation: CAC01442.2.
AL049712 Genomic DNA. Translation: CAC01443.1.
BC001960 mRNA. Translation: AAH01960.1.
AL050094 mRNA. Translation: CAB43266.1.
PIRT08743.
T13147.
RefSeqNP_008830.2.
NP_777280.1.
UniGeneHs.436405

3D structure databases

HSSPHSSP built from PDB template 1HQS based on UniProtKB P39126.
ModBaseSearch...

Protein-protein interaction databases

IntActO43837.

PTM databases

PhosphoSiteO43837.

Genome annotation databases

EnsemblENSG00000101365. Homo sapiens. [Contig view]
GeneID3420.
KEGGhsa:3420.
NMPDRfig|9606.3.peg.19736.

Organism-specific databases

H-InvDBHIX0015578.
HGNCHGNC:5385. IDH3B.
MIM604526. gene.
PharmGKBPA29633.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOVERGENO43837.

Enzyme and pathway databases

ReactomeREACT_1046. Pyruvate metabolism and TCA cycle.

Gene expression databases

CleanExHS_IDH3B.
GermOnlineENSG00000101365. Homo sapiens.

Family and domain databases

InterProIPR004434. IsoCit_DHase_NAD_mit.
IPR001804. IsoCit_IM_DHase.
[Graphical view]
Gene3DG3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHERPTHR11835. IDH_IMDH_dimeric. 1 hit.
PfamPF00180. Iso_dh. 1 hit.
[Graphical view]
TIGRFAMsTIGR00175. mito_nad_idh. 1 hit.
PROSITEPS00470. IDH_IMDH. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

DrugBankDB00157. NADH.
NextBio13482.
SOURCESearch...

Entry information

Entry nameIDH3B_HUMAN
AccessionPrimary (citable) accession number: O43837
Secondary accession number(s): O95106 expand/collapse secondary AC list , Q5JXS8, Q9NQ06, Q9NQ07, Q9UEX0, Q9UG99
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: May 1, 2007
Last modified: November 25, 2008
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

Relevant documents

Human chromosome 20

Human chromosome 20: entries, gene names and cross-references to MIM

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents