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Protein

Beta-1,3-galactosyltransferase 2

Gene

B3GALT2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Can also utilize substrates with a terminal galactose residue, albeit with lower efficiency. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins. Inactive towards substrates with terminal alpha-N-acetylglucosamine (alpha-GlcNAc) or alpha-N-acetylgalactosamine (alpha-GalNAc) residues.2 Publications

Catalytic activityi

UDP-alpha-D-galactose + N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide = UDP + beta-D-galactosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide.2 Publications

Cofactori

Mn2+By similarity

Kineticsi

  1. KM=90 µM for UDP-alpha-D-galactose1 Publication

    Pathwayi: protein glycosylation

    This protein is involved in the pathway protein glycosylation, which is part of Protein modification.
    View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

    GO - Molecular functioni

    GO - Biological processi

    • oligosaccharide biosynthetic process Source: Ensembl
    • protein glycosylation Source: ProtInc
    Complete GO annotation...

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Manganese

    Enzyme and pathway databases

    BioCyciZFISH:ENSG00000162630-MONOMER.
    BRENDAi2.4.1.62. 2681.
    UniPathwayiUPA00378.

    Protein family/group databases

    CAZyiGT31. Glycosyltransferase Family 31.

    Chemistry databases

    SwissLipidsiSLP:000000773.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Beta-1,3-galactosyltransferase 2 (EC:2.4.1.862 Publications)
    Short name:
    Beta-1,3-GalTase 2
    Short name:
    Beta3Gal-T2
    Short name:
    Beta3GalT2
    Alternative name(s):
    UDP-galactose:2-acetamido-2-deoxy-D-glucose 3beta-galactosyltransferase 2
    Gene namesi
    Name:B3GALT2
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    Proteomesi
    • UP000005640 Componenti: Chromosome 1

    Organism-specific databases

    HGNCiHGNC:917. B3GALT2.

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 24CytoplasmicSequence analysisAdd BLAST24
    Transmembranei25 – 45Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini46 – 422LumenalSequence analysisAdd BLAST377

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    Pathology & Biotechi

    Organism-specific databases

    DisGeNETi8707.
    OpenTargetsiENSG00000162630.
    PharmGKBiPA25210.

    Polymorphism and mutation databases

    BioMutaiB3GALT2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00002191501 – 422Beta-1,3-galactosyltransferase 2Add BLAST422

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi75N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi100N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi119N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
    Glycosylationi226N-linked (GlcNAc...)Sequence analysis1

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    PaxDbiO43825.
    PeptideAtlasiO43825.
    PRIDEiO43825.

    PTM databases

    iPTMnetiO43825.
    PhosphoSitePlusiO43825.

    Expressioni

    Tissue specificityi

    Detected in heart and brain.2 Publications

    Gene expression databases

    BgeeiENSG00000162630.
    CleanExiHS_B3GALT2.
    GenevisibleiO43825. HS.

    Organism-specific databases

    HPAiHPA055372.

    Interactioni

    Protein-protein interaction databases

    BioGridi114250. 10 interactors.
    IntActiO43825. 1 interactor.
    STRINGi9606.ENSP00000356404.

    Structurei

    3D structure databases

    ProteinModelPortaliO43825.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyltransferase 31 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG2287. Eukaryota.
    ENOG410ZZ1B. LUCA.
    GeneTreeiENSGT00760000118879.
    HOGENOMiHOG000059604.
    HOVERGENiHBG101354.
    InParanoidiO43825.
    KOiK07820.
    OMAiYTIRGFR.
    OrthoDBiEOG091G0AXM.
    PhylomeDBiO43825.
    TreeFamiTF318639.

    Family and domain databases

    InterProiIPR002659. Glyco_trans_31.
    [Graphical view]
    PANTHERiPTHR11214. PTHR11214. 1 hit.
    PfamiPF01762. Galactosyl_T. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43825-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MLQWRRRHCC FAKMTWNAKR SLFRTHLIGV LSLVFLFAMF LFFNHHDWLP
    60 70 80 90 100
    GRAGFKENPV TYTFRGFRST KSETNHSSLR NIWKETVPQT LRPQTATNSN
    110 120 130 140 150
    NTDLSPQGVT GLENTLSANG SIYNEKGTGH PNSYHFKYII NEPEKCQEKS
    160 170 180 190 200
    PFLILLIAAE PGQIEARRAI RQTWGNESLA PGIQITRIFL LGLSIKLNGY
    210 220 230 240 250
    LQRAILEESR QYHDIIQQEY LDTYYNLTIK TLMGMNWVAT YCPHIPYVMK
    260 270 280 290 300
    TDSDMFVNTE YLINKLLKPD LPPRHNYFTG YLMRGYAPNR NKDSKWYMPP
    310 320 330 340 350
    DLYPSERYPV FCSGTGYVFS GDLAEKIFKV SLGIRRLHLE DVYVGICLAK
    360 370 380 390 400
    LRIDPVPPPN EFVFNHWRVS YSSCKYSHLI TSHQFQPSEL IKYWNHLQQN
    410 420
    KHNACANAAK EKAGRYRHRK LH
    Length:422
    Mass (Da):49,213
    Last modified:June 1, 1998 - v1
    Checksum:iBA62942D6AFB4B0C
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti172Q → R in AAG60610 (PubMed:11318611).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y15014 mRNA. Translation: CAA75245.1.
    Y15060 mRNA. Translation: CAA75344.1.
    AF288390 mRNA. Translation: AAG60610.1.
    AK314116 mRNA. Translation: BAG36808.1.
    AL390863 Genomic DNA. Translation: CAH71590.1.
    CH471067 Genomic DNA. Translation: EAW91251.1.
    BC022507 mRNA. Translation: AAH22507.1.
    CCDSiCCDS1383.1.
    RefSeqiNP_003774.1. NM_003783.3.
    UniGeneiHs.518834.

    Genome annotation databases

    EnsembliENST00000367434; ENSP00000356404; ENSG00000162630.
    GeneIDi8707.
    KEGGihsa:8707.
    UCSCiuc001gtc.5. human.

    Cross-referencesi

    Web resourcesi

    Functional Glycomics Gateway - GTase

    Beta-1,3-galactosyltransferase 2

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    Y15014 mRNA. Translation: CAA75245.1.
    Y15060 mRNA. Translation: CAA75344.1.
    AF288390 mRNA. Translation: AAG60610.1.
    AK314116 mRNA. Translation: BAG36808.1.
    AL390863 Genomic DNA. Translation: CAH71590.1.
    CH471067 Genomic DNA. Translation: EAW91251.1.
    BC022507 mRNA. Translation: AAH22507.1.
    CCDSiCCDS1383.1.
    RefSeqiNP_003774.1. NM_003783.3.
    UniGeneiHs.518834.

    3D structure databases

    ProteinModelPortaliO43825.
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi114250. 10 interactors.
    IntActiO43825. 1 interactor.
    STRINGi9606.ENSP00000356404.

    Chemistry databases

    SwissLipidsiSLP:000000773.

    Protein family/group databases

    CAZyiGT31. Glycosyltransferase Family 31.

    PTM databases

    iPTMnetiO43825.
    PhosphoSitePlusiO43825.

    Polymorphism and mutation databases

    BioMutaiB3GALT2.

    Proteomic databases

    PaxDbiO43825.
    PeptideAtlasiO43825.
    PRIDEiO43825.

    Protocols and materials databases

    DNASUi8707.
    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENST00000367434; ENSP00000356404; ENSG00000162630.
    GeneIDi8707.
    KEGGihsa:8707.
    UCSCiuc001gtc.5. human.

    Organism-specific databases

    CTDi8707.
    DisGeNETi8707.
    GeneCardsiB3GALT2.
    HGNCiHGNC:917. B3GALT2.
    HPAiHPA055372.
    MIMi603018. gene.
    neXtProtiNX_O43825.
    OpenTargetsiENSG00000162630.
    PharmGKBiPA25210.
    GenAtlasiSearch...

    Phylogenomic databases

    eggNOGiKOG2287. Eukaryota.
    ENOG410ZZ1B. LUCA.
    GeneTreeiENSGT00760000118879.
    HOGENOMiHOG000059604.
    HOVERGENiHBG101354.
    InParanoidiO43825.
    KOiK07820.
    OMAiYTIRGFR.
    OrthoDBiEOG091G0AXM.
    PhylomeDBiO43825.
    TreeFamiTF318639.

    Enzyme and pathway databases

    UniPathwayiUPA00378.
    BioCyciZFISH:ENSG00000162630-MONOMER.
    BRENDAi2.4.1.62. 2681.

    Miscellaneous databases

    GenomeRNAii8707.
    PROiO43825.
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSG00000162630.
    CleanExiHS_B3GALT2.
    GenevisibleiO43825. HS.

    Family and domain databases

    InterProiIPR002659. Glyco_trans_31.
    [Graphical view]
    PANTHERiPTHR11214. PTHR11214. 1 hit.
    PfamiPF01762. Galactosyl_T. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiB3GT2_HUMAN
    AccessioniPrimary (citable) accession number: O43825
    Secondary accession number(s): B2RAB1, Q9BZQ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 15, 2005
    Last sequence update: June 1, 1998
    Last modified: November 30, 2016
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 1
      Human chromosome 1: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    4. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.