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O43823

- AKAP8_HUMAN

UniProt

O43823 - AKAP8_HUMAN

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Protein

A-kinase anchor protein 8

Gene

AKAP8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri390 – 41425C2H2 AKAP95-type 1Add
BLAST
Zinc fingeri479 – 50426C2H2 AKAP95-type 2Add
BLAST

GO - Molecular functioni

  1. double-stranded DNA binding Source: Ensembl
  2. poly(A) RNA binding Source: UniProtKB
  3. zinc ion binding Source: Ensembl

GO - Biological processi

  1. mitotic chromosome condensation Source: Ensembl
  2. mitotic nuclear division Source: ProtInc
  3. signal transduction Source: ProtInc
Complete GO annotation...

Keywords - Ligandi

DNA-binding, Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 8
Short name:
AKAP-8
Alternative name(s):
A-kinase anchor protein 95 kDa
Short name:
AKAP 95
Gene namesi
Name:AKAP8
Synonyms:AKAP95
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 19

Organism-specific databases

HGNCiHGNC:378. AKAP8.

Subcellular locationi

Nucleus matrix
Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.

GO - Cellular componenti

  1. condensed chromosome Source: Ensembl
  2. female pronucleus Source: Ensembl
  3. Golgi apparatus Source: Ensembl
  4. membrane Source: UniProtKB
  5. mitochondrion Source: Ensembl
  6. nuclear matrix Source: UniProtKB
  7. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24672.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 692692A-kinase anchor protein 8PRO_0000075381Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei112 – 1121Phosphoserine1 Publication
Modified residuei323 – 3231Phosphoserine5 Publications
Modified residuei328 – 3281Phosphoserine6 Publications
Modified residuei339 – 3391Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO43823.
PaxDbiO43823.
PeptideAtlasiO43823.
PRIDEiO43823.

PTM databases

PhosphoSiteiO43823.

Expressioni

Tissue specificityi

Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.

Gene expression databases

BgeeiO43823.
CleanExiHS_AKAP8.
ExpressionAtlasiO43823. baseline and differential.
GenevestigatoriO43823.

Organism-specific databases

HPAiHPA004776.

Interactioni

Subunit structurei

Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis. Interacts (via C-terminus) with FIGN By similarity.By similarity

Protein-protein interaction databases

BioGridi115561. 32 interactions.
DIPiDIP-38194N.
IntActiO43823. 14 interactions.
MINTiMINT-2796393.
STRINGi9606.ENSP00000269701.

Structurei

3D structure databases

ProteinModelPortaliO43823.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni572 – 58918RII-bindingBy similarityAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi368 – 37710Nuclear localization signalSequence Analysis

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi107 – 11812Poly-GlyAdd
BLAST

Sequence similaritiesi

Belongs to the AKAP95 family.Curated
Contains 2 C2H2 AKAP95-type zinc fingers.Curated

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri390 – 41425C2H2 AKAP95-type 1Add
BLAST
Zinc fingeri479 – 50426C2H2 AKAP95-type 2Add
BLAST

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG47695.
GeneTreeiENSGT00530000063777.
HOGENOMiHOG000033876.
HOVERGENiHBG053198.
InParanoidiO43823.
KOiK16525.
OMAiHSVDHNH.
OrthoDBiEOG741Z1X.
PhylomeDBiO43823.
TreeFamiTF105407.

Family and domain databases

InterProiIPR007071. AKAP95.
IPR015880. Znf_C2H2-like.
[Graphical view]
PANTHERiPTHR12190. PTHR12190. 1 hit.
PfamiPF04988. AKAP95. 1 hit.
[Graphical view]
SMARTiSM00355. ZnF_C2H2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O43823-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDQGYGGYGA WSAGPANTQG AYGTGVASWQ GYENYNYYGA QNTSVTTGAT
60 70 80 90 100
YSYGPASWEA AKANDGGLAA GAPAMHMASY GPEPCTDNSD SLIAKINQRL
110 120 130 140 150
DMMSKEGGRG GSGGGGEGIQ DRESSFRFQP FESYDSRPCL PEHNPYRPSY
160 170 180 190 200
SYDYEFDLGS DRNGSFGGQY SECRDPARER GSLDGFMRGR GQGRFQDRSN
210 220 230 240 250
PGTFMRSDPF VPPAASSEPL STPWNELNYV GGRGLGGPSP SRPPPSLFSQ
260 270 280 290 300
SMAPDYGVMG MQGAGGYDST MPYGCGRSQP RMRDRDRPKR RGFDRFGPDG
310 320 330 340 350
TGRKRKQFQL YEEPDTKLAR VDSEGDFSEN DDAAGDFRSG DEEFKGEDEL
360 370 380 390 400
CDSGRQRGEK EDEDEDVKKR REKQRRRDRT RDRAADRIQF ACSVCKFRSF
410 420 430 440 450
DDEEIQKHLQ SKFHKETLRF ISTKLPDKTV EFLQEYIVNR NKKIEKRRQE
460 470 480 490 500
LMEKETAKPK PDPFKGIGQE HFFKKIEAAH CLACDMLIPA QPQLLQRHLH
510 520 530 540 550
SVDHNHNRRL AAEQFKKTSL HVAKSVLNNR HIVKMLEKYL KGEDPFTSET
560 570 580 590 600
VDPEMEGDDN LGGEDKKETP EEVAADVLAE VITAAVRAVD GEGAPAPESS
610 620 630 640 650
GEPAEDEGPT DTAEAGSDPQ AEQLLEEQVP CGTAHEKGVP KARSEAAEAG
660 670 680 690
NGAETMAAEA ESAQTRVAPA PAAADAEVEQ TDAESKDAVP TE
Length:692
Mass (Da):76,108
Last modified:June 1, 1998 - v1
Checksum:iCBCD5F014FD94B66
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti664 – 6641Q → H in a breast cancer sample; somatic mutation. 1 Publication
VAR_036534

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11997 mRNA. Translation: CAA72722.1.
AC005785 Genomic DNA. Translation: AAC62838.1.
CCDSiCCDS12329.1.
PIRiT13161.
RefSeqiNP_005849.1. NM_005858.3.
UniGeneiHs.594496.

Genome annotation databases

EnsembliENST00000269701; ENSP00000269701; ENSG00000105127.
GeneIDi10270.
KEGGihsa:10270.
UCSCiuc002nav.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y11997 mRNA. Translation: CAA72722.1 .
AC005785 Genomic DNA. Translation: AAC62838.1 .
CCDSi CCDS12329.1.
PIRi T13161.
RefSeqi NP_005849.1. NM_005858.3.
UniGenei Hs.594496.

3D structure databases

ProteinModelPortali O43823.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 115561. 32 interactions.
DIPi DIP-38194N.
IntActi O43823. 14 interactions.
MINTi MINT-2796393.
STRINGi 9606.ENSP00000269701.

PTM databases

PhosphoSitei O43823.

Proteomic databases

MaxQBi O43823.
PaxDbi O43823.
PeptideAtlasi O43823.
PRIDEi O43823.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000269701 ; ENSP00000269701 ; ENSG00000105127 .
GeneIDi 10270.
KEGGi hsa:10270.
UCSCi uc002nav.3. human.

Organism-specific databases

CTDi 10270.
GeneCardsi GC19M015464.
HGNCi HGNC:378. AKAP8.
HPAi HPA004776.
MIMi 604692. gene.
neXtProti NX_O43823.
PharmGKBi PA24672.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG47695.
GeneTreei ENSGT00530000063777.
HOGENOMi HOG000033876.
HOVERGENi HBG053198.
InParanoidi O43823.
KOi K16525.
OMAi HSVDHNH.
OrthoDBi EOG741Z1X.
PhylomeDBi O43823.
TreeFami TF105407.

Miscellaneous databases

GeneWikii AKAP8.
GenomeRNAii 10270.
NextBioi 38910.
PROi O43823.
SOURCEi Search...

Gene expression databases

Bgeei O43823.
CleanExi HS_AKAP8.
ExpressionAtlasi O43823. baseline and differential.
Genevestigatori O43823.

Family and domain databases

InterProi IPR007071. AKAP95.
IPR015880. Znf_C2H2-like.
[Graphical view ]
PANTHERi PTHR12190. PTHR12190. 1 hit.
Pfami PF04988. AKAP95. 1 hit.
[Graphical view ]
SMARTi SM00355. ZnF_C2H2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95."
    Eide T., Coghlan V., Oerstavik S., Holsve C., Solberg R., Skaelhegg B.S., Lamb N.J.C., Langeberg L., Fernandez A., Scott J.D., Jahnsen T., Tasken K.
    Exp. Cell Res. 238:305-316(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Cerebellum and Testis.
  2. "The DNA sequence and biology of human chromosome 19."
    Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
    , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
    Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112; SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-664.

Entry informationi

Entry nameiAKAP8_HUMAN
AccessioniPrimary (citable) accession number: O43823
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: June 1, 1998
Last modified: October 29, 2014
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3