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O43823

- AKAP8_HUMAN

UniProt

O43823 - AKAP8_HUMAN

Protein

A-kinase anchor protein 8

Gene

AKAP8

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 129 (01 Oct 2014)
      Sequence version 1 (01 Jun 1998)
      Previous versions | rss
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    Functioni

    Anchoring protein that mediates the subcellular compartmentation of cAMP-dependent protein kinase (PKA type II).

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri390 – 41425C2H2 AKAP95-type 1Add
    BLAST
    Zinc fingeri479 – 50426C2H2 AKAP95-type 2Add
    BLAST

    GO - Molecular functioni

    1. double-stranded DNA binding Source: Ensembl
    2. poly(A) RNA binding Source: UniProtKB
    3. zinc ion binding Source: Ensembl

    GO - Biological processi

    1. mitotic chromosome condensation Source: Ensembl
    2. mitotic nuclear division Source: ProtInc
    3. signal transduction Source: ProtInc

    Keywords - Ligandi

    DNA-binding, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    A-kinase anchor protein 8
    Short name:
    AKAP-8
    Alternative name(s):
    A-kinase anchor protein 95 kDa
    Short name:
    AKAP 95
    Gene namesi
    Name:AKAP8
    Synonyms:AKAP95
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 19

    Organism-specific databases

    HGNCiHGNC:378. AKAP8.

    Subcellular locationi

    Nucleus matrix
    Note: Associated with the nuclear matrix. Redistributed and detached from condensed chromatin during mitosis.

    GO - Cellular componenti

    1. condensed chromosome Source: Ensembl
    2. female pronucleus Source: Ensembl
    3. Golgi apparatus Source: Ensembl
    4. membrane Source: UniProtKB
    5. mitochondrion Source: Ensembl
    6. nuclear matrix Source: UniProtKB
    7. nucleus Source: HPA

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA24672.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 692692A-kinase anchor protein 8PRO_0000075381Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei112 – 1121Phosphoserine1 Publication
    Modified residuei323 – 3231Phosphoserine5 Publications
    Modified residuei328 – 3281Phosphoserine6 Publications
    Modified residuei339 – 3391Phosphoserine3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO43823.
    PaxDbiO43823.
    PeptideAtlasiO43823.
    PRIDEiO43823.

    PTM databases

    PhosphoSiteiO43823.

    Expressioni

    Tissue specificityi

    Highly expressed in heart, liver, skeletal muscle, kidney and pancreas.

    Gene expression databases

    ArrayExpressiO43823.
    BgeeiO43823.
    CleanExiHS_AKAP8.
    GenevestigatoriO43823.

    Organism-specific databases

    HPAiHPA004776.

    Interactioni

    Subunit structurei

    Binds to dimeric RII-alpha regulatory subunit of PKA during mitosis. Interacts (via C-terminus) with FIGN By similarity.By similarity

    Protein-protein interaction databases

    BioGridi115561. 31 interactions.
    DIPiDIP-38194N.
    IntActiO43823. 14 interactions.
    MINTiMINT-2796393.
    STRINGi9606.ENSP00000269701.

    Structurei

    3D structure databases

    ProteinModelPortaliO43823.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni572 – 58918RII-bindingBy similarityAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi368 – 37710Nuclear localization signalSequence Analysis

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi107 – 11812Poly-GlyAdd
    BLAST

    Sequence similaritiesi

    Belongs to the AKAP95 family.Curated
    Contains 2 C2H2 AKAP95-type zinc fingers.Curated

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri390 – 41425C2H2 AKAP95-type 1Add
    BLAST
    Zinc fingeri479 – 50426C2H2 AKAP95-type 2Add
    BLAST

    Keywords - Domaini

    Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG47695.
    HOGENOMiHOG000033876.
    HOVERGENiHBG053198.
    InParanoidiO43823.
    KOiK16525.
    OMAiHSVDHNH.
    OrthoDBiEOG741Z1X.
    PhylomeDBiO43823.
    TreeFamiTF105407.

    Family and domain databases

    InterProiIPR007071. AKAP95.
    IPR015880. Znf_C2H2-like.
    [Graphical view]
    PANTHERiPTHR12190. PTHR12190. 1 hit.
    PfamiPF04988. AKAP95. 1 hit.
    [Graphical view]
    SMARTiSM00355. ZnF_C2H2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O43823-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQGYGGYGA WSAGPANTQG AYGTGVASWQ GYENYNYYGA QNTSVTTGAT    50
    YSYGPASWEA AKANDGGLAA GAPAMHMASY GPEPCTDNSD SLIAKINQRL 100
    DMMSKEGGRG GSGGGGEGIQ DRESSFRFQP FESYDSRPCL PEHNPYRPSY 150
    SYDYEFDLGS DRNGSFGGQY SECRDPARER GSLDGFMRGR GQGRFQDRSN 200
    PGTFMRSDPF VPPAASSEPL STPWNELNYV GGRGLGGPSP SRPPPSLFSQ 250
    SMAPDYGVMG MQGAGGYDST MPYGCGRSQP RMRDRDRPKR RGFDRFGPDG 300
    TGRKRKQFQL YEEPDTKLAR VDSEGDFSEN DDAAGDFRSG DEEFKGEDEL 350
    CDSGRQRGEK EDEDEDVKKR REKQRRRDRT RDRAADRIQF ACSVCKFRSF 400
    DDEEIQKHLQ SKFHKETLRF ISTKLPDKTV EFLQEYIVNR NKKIEKRRQE 450
    LMEKETAKPK PDPFKGIGQE HFFKKIEAAH CLACDMLIPA QPQLLQRHLH 500
    SVDHNHNRRL AAEQFKKTSL HVAKSVLNNR HIVKMLEKYL KGEDPFTSET 550
    VDPEMEGDDN LGGEDKKETP EEVAADVLAE VITAAVRAVD GEGAPAPESS 600
    GEPAEDEGPT DTAEAGSDPQ AEQLLEEQVP CGTAHEKGVP KARSEAAEAG 650
    NGAETMAAEA ESAQTRVAPA PAAADAEVEQ TDAESKDAVP TE 692
    Length:692
    Mass (Da):76,108
    Last modified:June 1, 1998 - v1
    Checksum:iCBCD5F014FD94B66
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti664 – 6641Q → H in a breast cancer sample; somatic mutation. 1 Publication
    VAR_036534

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11997 mRNA. Translation: CAA72722.1.
    AC005785 Genomic DNA. Translation: AAC62838.1.
    CCDSiCCDS12329.1.
    PIRiT13161.
    RefSeqiNP_005849.1. NM_005858.3.
    UniGeneiHs.594496.

    Genome annotation databases

    EnsembliENST00000269701; ENSP00000269701; ENSG00000105127.
    GeneIDi10270.
    KEGGihsa:10270.
    UCSCiuc002nav.3. human.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y11997 mRNA. Translation: CAA72722.1 .
    AC005785 Genomic DNA. Translation: AAC62838.1 .
    CCDSi CCDS12329.1.
    PIRi T13161.
    RefSeqi NP_005849.1. NM_005858.3.
    UniGenei Hs.594496.

    3D structure databases

    ProteinModelPortali O43823.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 115561. 31 interactions.
    DIPi DIP-38194N.
    IntActi O43823. 14 interactions.
    MINTi MINT-2796393.
    STRINGi 9606.ENSP00000269701.

    PTM databases

    PhosphoSitei O43823.

    Proteomic databases

    MaxQBi O43823.
    PaxDbi O43823.
    PeptideAtlasi O43823.
    PRIDEi O43823.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000269701 ; ENSP00000269701 ; ENSG00000105127 .
    GeneIDi 10270.
    KEGGi hsa:10270.
    UCSCi uc002nav.3. human.

    Organism-specific databases

    CTDi 10270.
    GeneCardsi GC19M015464.
    HGNCi HGNC:378. AKAP8.
    HPAi HPA004776.
    MIMi 604692. gene.
    neXtProti NX_O43823.
    PharmGKBi PA24672.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG47695.
    HOGENOMi HOG000033876.
    HOVERGENi HBG053198.
    InParanoidi O43823.
    KOi K16525.
    OMAi HSVDHNH.
    OrthoDBi EOG741Z1X.
    PhylomeDBi O43823.
    TreeFami TF105407.

    Miscellaneous databases

    GeneWikii AKAP8.
    GenomeRNAii 10270.
    NextBioi 38910.
    PROi O43823.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O43823.
    Bgeei O43823.
    CleanExi HS_AKAP8.
    Genevestigatori O43823.

    Family and domain databases

    InterProi IPR007071. AKAP95.
    IPR015880. Znf_C2H2-like.
    [Graphical view ]
    PANTHERi PTHR12190. PTHR12190. 1 hit.
    Pfami PF04988. AKAP95. 1 hit.
    [Graphical view ]
    SMARTi SM00355. ZnF_C2H2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning, chromosomal localization, and cell cycle-dependent subcellular distribution of the A-kinase anchoring protein, AKAP95."
      Eide T., Coghlan V., Oerstavik S., Holsve C., Solberg R., Skaelhegg B.S., Lamb N.J.C., Langeberg L., Fernandez A., Scott J.D., Jahnsen T., Tasken K.
      Exp. Cell Res. 238:305-316(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Cerebellum and Testis.
    2. "The DNA sequence and biology of human chromosome 19."
      Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V.
      , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.
      Nature 428:529-535(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    3. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
      Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
      Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    4. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
      Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
      Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
      Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
      Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    8. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
      Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
      Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-323 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Cervix carcinoma.
    9. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
      Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
      Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-112; SER-323; SER-328 AND SER-339, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. Cited for: VARIANT [LARGE SCALE ANALYSIS] HIS-664.

    Entry informationi

    Entry nameiAKAP8_HUMAN
    AccessioniPrimary (citable) accession number: O43823
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: June 1, 1998
    Last modified: October 1, 2014
    This is version 129 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 19
      Human chromosome 19: entries, gene names and cross-references to MIM
    2. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    3. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    4. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3