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Protein

Alpha-endosulfine

Gene

ENSA

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protein phosphatase inhibitor that specifically inhibits protein phosphatase 2A (PP2A) during mitosis. When phosphorylated at Ser-67 during mitosis, specifically interacts with PPP2R2D (PR55-delta) and inhibits its activity, leading to inactivation of PP2A, an essential condition to keep cyclin-B1-CDK1 activity high during M phase (By similarity). Also acts as a stimulator of insulin secretion by interacting with sulfonylurea receptor (ABCC8), thereby preventing sulfonylurea from binding to its receptor and reducing K(ATP) channel currents.By similarity1 Publication

GO - Molecular functioni

  • ion channel inhibitor activity Source: ProtInc
  • phosphatase inhibitor activity Source: UniProtKB
  • potassium channel inhibitor activity Source: MGI
  • protein phosphatase 2A binding Source: UniProtKB
  • protein phosphatase inhibitor activity Source: GO_Central
  • protein phosphatase type 2A regulator activity Source: UniProtKB
  • receptor binding Source: MGI

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • G2/M transition of mitotic cell cycle Source: UniProtKB
  • mitotic nuclear division Source: UniProtKB
  • negative regulation of protein dephosphorylation Source: GO_Central
  • regulation of insulin secretion Source: MGI
  • response to glucose Source: Ensembl
  • response to nutrient Source: ProtInc
  • transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Protein phosphatase inhibitor

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143420-MONOMER.
ReactomeiR-HSA-2465910. MASTL Facilitates Mitotic Progression.
SIGNORiO43768.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-endosulfine
Alternative name(s):
ARPP-19e
Gene namesi
Name:ENSA
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 1

Organism-specific databases

HGNCiHGNC:3360. ENSA.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi109S → E: Mimicks a phosphorylated state and impairs interaction with SNCA. 1 Publication1

Organism-specific databases

DisGeNETi2029.
OpenTargetsiENSG00000143420.
PharmGKBiPA27796.

Polymorphism and mutation databases

BioMutaiENSA.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001467582 – 121Alpha-endosulfineAdd BLAST120

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei2PhosphoserineCombined sources1
Modified residuei21PhosphothreonineCombined sources1
Modified residuei43PhosphoserineCombined sources1
Modified residuei67Phosphoserine; by GWL1 Publication1
Modified residuei109Phosphoserine; by PKACombined sources1 Publication1

Post-translational modificationi

Phosphorylation at Ser-67 by GWL during mitosis is essential for interaction with PPP2R2D (PR55-delta) and subsequent inactivation of PP2A (By similarity). Phosphorylated by PKA.By similarity2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiO43768.
MaxQBiO43768.
PeptideAtlasiO43768.
PRIDEiO43768.
TopDownProteomicsiO43768-1. [O43768-1]
O43768-2. [O43768-2]
O43768-3. [O43768-3]
O43768-5. [O43768-5]

PTM databases

iPTMnetiO43768.
PhosphoSitePlusiO43768.

Expressioni

Tissue specificityi

Widely expressed with high levels in skeletal muscle and brain and lower levels in the pancreas.2 Publications

Gene expression databases

BgeeiENSG00000143420.
CleanExiHS_ENSA.
ExpressionAtlasiO43768. baseline and differential.
GenevisibleiO43768. HS.

Organism-specific databases

HPAiHPA051294.

Interactioni

Subunit structurei

Interacts (when phosphorylated at Ser-67) with PPP2R2D (By similarity). Interacts with ABCC8. Interacts with SNCA; interaction is disrupted when phosphorylated at Ser-109.By similarity2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ACDQ96AP02EBI-714511,EBI-717666

GO - Molecular functioni

  • protein phosphatase 2A binding Source: UniProtKB
  • receptor binding Source: MGI

Protein-protein interaction databases

BioGridi108343. 28 interactors.
IntActiO43768. 7 interactors.
MINTiMINT-1431607.

Structurei

3D structure databases

ProteinModelPortaliO43768.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the endosulfine family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000010139.
HOVERGENiHBG000297.
InParanoidiO43768.
PhylomeDBiO43768.
TreeFamiTF314718.

Family and domain databases

InterProiIPR006760. Endosulphine.
[Graphical view]
PfamiPF04667. Endosulfine. 1 hit.
[Graphical view]

Sequences (9)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 9 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O43768-1) [UniParc]FASTAAdd to basket
Also known as: Alpha

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSQKQEEENP AEETGEEKQD TQEKEGILPE RAEEAKLKAK YPSLGQKPGG
60 70 80 90 100
SDFLMKRLQK GQKYFDSGDY NMAKAKMKNK QLPSAGPDKN LVTGDHIPTP
110 120
QDLPQRKSSL VTSKLAGGQV E
Length:121
Mass (Da):13,389
Last modified:June 1, 1998 - v1
Checksum:i7C76315AA17E7542
GO
Isoform 2 (identifier: O43768-2) [UniParc]FASTAAdd to basket
Also known as: Beta

The sequence of this isoform differs from the canonical sequence as follows:
     118-121: Missing.

Show »
Length:117
Mass (Da):12,976
Checksum:iD17E754219767472
GO
Isoform 3 (identifier: O43768-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: G → GDYKSLHWSVLLCADEM

Show »
Length:137
Mass (Da):15,281
Checksum:iE9CF169C48A1FC1B
GO
Isoform 4 (identifier: O43768-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: G → GVWGIASYPLSLGLKEVLRMKSVE
     118-121: Missing.

Show »
Length:140
Mass (Da):15,534
Checksum:i2AB6570AFD91E54D
GO
Isoform 5 (identifier: O43768-5) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.
     5-19: QEEENPAEETGEEKQ → MAGGLGCDVCYWFVE

Show »
Length:117
Mass (Da):12,818
Checksum:iEEE3B2739FD70FC2
GO
Isoform 6 (identifier: O43768-6) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.
     5-19: QEEENPAEETGEEKQ → MAGGLGCDVCYWFVE
     118-121: Missing.

Show »
Length:113
Mass (Da):12,404
Checksum:iCFD70FC2E5FE760C
GO
Isoform 7 (identifier: O43768-7) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-4: Missing.
     5-19: QEEENPAEETGEEKQ → MAGGLGCDVCYWFVE
     61-61: G → GDYKSLHWSVLLCADEM

Show »
Length:133
Mass (Da):14,710
Checksum:i852736F0B466D4AB
GO
Isoform 8 (identifier: O43768-8) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     62-121: QKYFDSGDYN...TSKLAGGQVE → VWGIVSYPLS...LNRSRGEFEI

Show »
Length:105
Mass (Da):11,990
Checksum:iE6306F60528ECCA4
GO
Isoform 9 (identifier: O43768-9) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     61-61: G → GDYKSLHWSVLLCADEM
     118-121: Missing.

Note: No experimental confirmation available.
Show »
Length:133
Mass (Da):14,868
Checksum:i28A1FC1BE09A3895
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5Q → R in CAG38815 (Ref. 8) Curated1
Sequence conflicti21T → M in BAF82753 (PubMed:14702039).Curated1
Sequence conflicti22Q → L in AAH68544 (PubMed:15489334).Curated1
Sequence conflicti114K → Q in CAG33411 (Ref. 8) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0370631 – 4Missing in isoform 5, isoform 6 and isoform 7. 1 Publication4
Alternative sequenceiVSP_0370645 – 19QEEEN…GEEKQ → MAGGLGCDVCYWFVE in isoform 5, isoform 6 and isoform 7. 1 PublicationAdd BLAST15
Alternative sequenceiVSP_03706561G → GDYKSLHWSVLLCADEM in isoform 3, isoform 7 and isoform 9. 1 Publication1
Alternative sequenceiVSP_03706661G → GVWGIASYPLSLGLKEVLRM KSVE in isoform 4. 1 Publication1
Alternative sequenceiVSP_03706762 – 121QKYFD…GGQVE → VWGIVSYPLSLELKEVLRMK SVEVLLDPFLEVLLLNRSRG EFEI in isoform 8. CuratedAdd BLAST60
Alternative sequenceiVSP_001443118 – 121Missing in isoform 2, isoform 4, isoform 6 and isoform 9. 5 Publications4

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99906 mRNA. Translation: CAA68180.1.
AJ010966 Genomic DNA. Translation: CAB65125.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73827.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73828.1.
AY326402, AY326400, AY326401 Genomic DNA. Translation: AAQ73829.1.
AY326401, AY326400 Genomic DNA. Translation: AAQ73830.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73831.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73832.1.
AF067170 mRNA. Translation: AAD32454.1.
AF157509 mRNA. Translation: AAF80340.1.
AF157510 mRNA. Translation: AAF80341.1.
AK001981 mRNA. Translation: BAG50998.1.
AK290064 mRNA. Translation: BAF82753.1.
DA888224 mRNA. No translation available.
CR749580 mRNA. Translation: CAH18372.1.
CR457130 mRNA. Translation: CAG33411.1.
CR536578 mRNA. Translation: CAG38815.1.
AL356356 Genomic DNA. Translation: CAI15505.1.
AL356356 Genomic DNA. Translation: CAI15506.1.
AL356356 Genomic DNA. Translation: CAI15507.1.
AL356356 Genomic DNA. Translation: CAI15509.1.
AL356356 Genomic DNA. Translation: CAI15510.1.
AL356356 Genomic DNA. Translation: CAI15511.1.
AL356356 Genomic DNA. Translation: CAI15512.1.
CH471121 Genomic DNA. Translation: EAW53528.1.
CH471121 Genomic DNA. Translation: EAW53529.1.
CH471121 Genomic DNA. Translation: EAW53530.1.
CH471121 Genomic DNA. Translation: EAW53532.1.
CH471121 Genomic DNA. Translation: EAW53533.1.
BC000436 mRNA. Translation: AAH00436.1.
BC004461 mRNA. Translation: AAH04461.1.
BC068544 mRNA. Translation: AAH68544.1.
BC069208 mRNA. Translation: AAH69208.1.
CCDSiCCDS958.1. [O43768-1]
CCDS959.1. [O43768-3]
CCDS960.1. [O43768-7]
CCDS961.1. [O43768-5]
CCDS962.1. [O43768-9]
CCDS963.1. [O43768-2]
CCDS964.1. [O43768-6]
CCDS965.1. [O43768-8]
RefSeqiNP_004427.1. NM_004436.2. [O43768-1]
NP_996925.1. NM_207042.1. [O43768-3]
NP_996926.1. NM_207043.1. [O43768-9]
NP_996927.1. NM_207044.1. [O43768-2]
NP_996928.1. NM_207045.1. [O43768-7]
NP_996929.1. NM_207046.1. [O43768-5]
NP_996930.1. NM_207047.1. [O43768-6]
NP_997051.1. NM_207168.1. [O43768-8]
UniGeneiHs.632456.

Genome annotation databases

EnsembliENST00000271690; ENSP00000271690; ENSG00000143420. [O43768-2]
ENST00000339643; ENSP00000341743; ENSG00000143420. [O43768-3]
ENST00000361532; ENSP00000354835; ENSG00000143420. [O43768-5]
ENST00000361631; ENSP00000355239; ENSG00000143420. [O43768-7]
ENST00000362052; ENSP00000355220; ENSG00000143420. [O43768-8]
ENST00000369014; ENSP00000358010; ENSG00000143420. [O43768-1]
ENST00000503241; ENSP00000424242; ENSG00000143420. [O43768-9]
ENST00000503345; ENSP00000421458; ENSG00000143420. [O43768-8]
ENST00000509582; ENSP00000426110; ENSG00000143420. [O43768-8]
ENST00000513281; ENSP00000422343; ENSG00000143420. [O43768-6]
GeneIDi2029.
KEGGihsa:2029.
UCSCiuc001evb.4. human. [O43768-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X99906 mRNA. Translation: CAA68180.1.
AJ010966 Genomic DNA. Translation: CAB65125.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73827.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73828.1.
AY326402, AY326400, AY326401 Genomic DNA. Translation: AAQ73829.1.
AY326401, AY326400 Genomic DNA. Translation: AAQ73830.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73831.1.
AY326403
, AY326400, AY326401, AY326402 Genomic DNA. Translation: AAQ73832.1.
AF067170 mRNA. Translation: AAD32454.1.
AF157509 mRNA. Translation: AAF80340.1.
AF157510 mRNA. Translation: AAF80341.1.
AK001981 mRNA. Translation: BAG50998.1.
AK290064 mRNA. Translation: BAF82753.1.
DA888224 mRNA. No translation available.
CR749580 mRNA. Translation: CAH18372.1.
CR457130 mRNA. Translation: CAG33411.1.
CR536578 mRNA. Translation: CAG38815.1.
AL356356 Genomic DNA. Translation: CAI15505.1.
AL356356 Genomic DNA. Translation: CAI15506.1.
AL356356 Genomic DNA. Translation: CAI15507.1.
AL356356 Genomic DNA. Translation: CAI15509.1.
AL356356 Genomic DNA. Translation: CAI15510.1.
AL356356 Genomic DNA. Translation: CAI15511.1.
AL356356 Genomic DNA. Translation: CAI15512.1.
CH471121 Genomic DNA. Translation: EAW53528.1.
CH471121 Genomic DNA. Translation: EAW53529.1.
CH471121 Genomic DNA. Translation: EAW53530.1.
CH471121 Genomic DNA. Translation: EAW53532.1.
CH471121 Genomic DNA. Translation: EAW53533.1.
BC000436 mRNA. Translation: AAH00436.1.
BC004461 mRNA. Translation: AAH04461.1.
BC068544 mRNA. Translation: AAH68544.1.
BC069208 mRNA. Translation: AAH69208.1.
CCDSiCCDS958.1. [O43768-1]
CCDS959.1. [O43768-3]
CCDS960.1. [O43768-7]
CCDS961.1. [O43768-5]
CCDS962.1. [O43768-9]
CCDS963.1. [O43768-2]
CCDS964.1. [O43768-6]
CCDS965.1. [O43768-8]
RefSeqiNP_004427.1. NM_004436.2. [O43768-1]
NP_996925.1. NM_207042.1. [O43768-3]
NP_996926.1. NM_207043.1. [O43768-9]
NP_996927.1. NM_207044.1. [O43768-2]
NP_996928.1. NM_207045.1. [O43768-7]
NP_996929.1. NM_207046.1. [O43768-5]
NP_996930.1. NM_207047.1. [O43768-6]
NP_997051.1. NM_207168.1. [O43768-8]
UniGeneiHs.632456.

3D structure databases

ProteinModelPortaliO43768.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi108343. 28 interactors.
IntActiO43768. 7 interactors.
MINTiMINT-1431607.

PTM databases

iPTMnetiO43768.
PhosphoSitePlusiO43768.

Polymorphism and mutation databases

BioMutaiENSA.

Proteomic databases

EPDiO43768.
MaxQBiO43768.
PeptideAtlasiO43768.
PRIDEiO43768.
TopDownProteomicsiO43768-1. [O43768-1]
O43768-2. [O43768-2]
O43768-3. [O43768-3]
O43768-5. [O43768-5]

Protocols and materials databases

DNASUi2029.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000271690; ENSP00000271690; ENSG00000143420. [O43768-2]
ENST00000339643; ENSP00000341743; ENSG00000143420. [O43768-3]
ENST00000361532; ENSP00000354835; ENSG00000143420. [O43768-5]
ENST00000361631; ENSP00000355239; ENSG00000143420. [O43768-7]
ENST00000362052; ENSP00000355220; ENSG00000143420. [O43768-8]
ENST00000369014; ENSP00000358010; ENSG00000143420. [O43768-1]
ENST00000503241; ENSP00000424242; ENSG00000143420. [O43768-9]
ENST00000503345; ENSP00000421458; ENSG00000143420. [O43768-8]
ENST00000509582; ENSP00000426110; ENSG00000143420. [O43768-8]
ENST00000513281; ENSP00000422343; ENSG00000143420. [O43768-6]
GeneIDi2029.
KEGGihsa:2029.
UCSCiuc001evb.4. human. [O43768-1]

Organism-specific databases

CTDi2029.
DisGeNETi2029.
GeneCardsiENSA.
HGNCiHGNC:3360. ENSA.
HPAiHPA051294.
MIMi603061. gene.
neXtProtiNX_O43768.
OpenTargetsiENSG00000143420.
PharmGKBiPA27796.
GenAtlasiSearch...

Phylogenomic databases

GeneTreeiENSGT00390000010139.
HOVERGENiHBG000297.
InParanoidiO43768.
PhylomeDBiO43768.
TreeFamiTF314718.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000143420-MONOMER.
ReactomeiR-HSA-2465910. MASTL Facilitates Mitotic Progression.
SIGNORiO43768.

Miscellaneous databases

ChiTaRSiENSA. human.
GeneWikiiENSA_(gene).
GenomeRNAii2029.
PROiO43768.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000143420.
CleanExiHS_ENSA.
ExpressionAtlasiO43768. baseline and differential.
GenevisibleiO43768. HS.

Family and domain databases

InterProiIPR006760. Endosulphine.
[Graphical view]
PfamiPF04667. Endosulfine. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiENSA_HUMAN
AccessioniPrimary (citable) accession number: O43768
Secondary accession number(s): A8K1Z9
, E9PB69, Q5T5H2, Q68D48, Q6FHW0, Q6IAM4, Q6NUL2, Q6VUC6, Q6VUC7, Q6VUC8, Q6VUC9, Q6VUD0, Q6VUD1, Q9NRZ0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: June 1, 1998
Last modified: November 30, 2016
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 1
    Human chromosome 1: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.