Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O43747

- AP1G1_HUMAN

UniProt

O43747 - AP1G1_HUMAN

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

AP-1 complex subunit gamma-1

Gene

AP1G1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

  1. GTP-dependent protein binding Source: BHF-UCL
  2. kinesin binding Source: UniProtKB
  3. protein transporter activity Source: InterPro
  4. Rab GTPase binding Source: BHF-UCL
  5. transporter activity Source: ProtInc

GO - Biological processi

  1. antigen processing and presentation of exogenous peptide antigen via MHC class II Source: Reactome
  2. endosome to melanosome transport Source: BHF-UCL
  3. Golgi to lysosome transport Source: UniProt
  4. intracellular protein transport Source: ProtInc
  5. melanosome organization Source: BHF-UCL
  6. membrane organization Source: Reactome
  7. positive regulation of natural killer cell degranulation Source: UniProt
  8. positive regulation of natural killer cell mediated cytotoxicity Source: UniProt
  9. post-Golgi vesicle-mediated transport Source: Reactome
  10. regulation of defense response to virus by virus Source: Reactome
  11. viral process Source: Reactome
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiREACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
REACT_121399. MHC class II antigen presentation.
REACT_19287. Lysosome Vesicle Biogenesis.
REACT_19400. Golgi Associated Vesicle Biogenesis.

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit gamma-1
Alternative name(s):
Adaptor protein complex AP-1 subunit gamma-1
Adaptor-related protein complex 1 subunit gamma-1
Clathrin assembly protein complex 1 gamma-1 large chain
Gamma1-adaptin
Golgi adaptor HA1/AP1 adaptin subunit gamma-1
Gene namesi
Name:AP1G1
Synonyms:ADTG, CLAPG1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 16

Organism-specific databases

HGNCiHGNC:555. AP1G1.

Subcellular locationi

Golgi apparatus 1 Publication. Cytoplasmic vesicleclathrin-coated vesicle membrane 1 Publication; Peripheral membrane protein 1 Publication; Cytoplasmic side 1 Publication
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

GO - Cellular componenti

  1. AP-type membrane coat adaptor complex Source: ProtInc
  2. clathrin adaptor complex Source: InterPro
  3. clathrin-coated vesicle Source: MGI
  4. clathrin-coated vesicle membrane Source: BHF-UCL
  5. cytoplasm Source: HPA
  6. cytoplasmic vesicle membrane Source: Reactome
  7. cytosol Source: Reactome
  8. Golgi apparatus Source: UniProtKB
  9. Golgi membrane Source: Reactome
  10. intracellular membrane-bounded organelle Source: HPA
  11. lysosomal membrane Source: Reactome
  12. membrane Source: UniProtKB
  13. perinuclear region of cytoplasm Source: Ensembl
  14. recycling endosome Source: UniProtKB
  15. trans-Golgi network membrane Source: Reactome
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA24845.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 822821AP-1 complex subunit gamma-1PRO_0000193758Add
BLAST

Proteomic databases

MaxQBiO43747.
PaxDbiO43747.
PRIDEiO43747.

PTM databases

PhosphoSiteiO43747.

Miscellaneous databases

PMAP-CutDBO43747.

Expressioni

Tissue specificityi

Widely expressed.

Gene expression databases

BgeeiO43747.
CleanExiHS_AP1G1.
ExpressionAtlasiO43747. baseline and differential.
GenevestigatoriO43747.

Organism-specific databases

HPAiCAB009049.
HPA041224.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain).3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RABEP1Q152762EBI-447609,EBI-447043

Protein-protein interaction databases

BioGridi106673. 31 interactions.
IntActiO43747. 20 interactions.
MINTiMINT-3369703.
STRINGi9606.ENSP00000377148.

Structurei

Secondary structure

1
822
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi707 – 7126
Beta strandi715 – 7239
Beta strandi730 – 73910
Beta strandi741 – 7433
Beta strandi745 – 7539
Beta strandi758 – 7625
Helixi772 – 7743
Beta strandi778 – 7858
Beta strandi795 – 8028
Beta strandi805 – 8128
Helixi818 – 8203

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1IU1X-ray1.80A/B677-822[»]
ProteinModelPortaliO43747.
SMRiO43747. Positions 1-589, 700-822.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43747.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini702 – 817116GAEPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 GAE domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG303101.
GeneTreeiENSGT00390000012618.
HOGENOMiHOG000210271.
HOVERGENiHBG067473.
InParanoidiO43747.
KOiK12391.
OrthoDBiEOG7S7SD7.
PhylomeDBiO43747.
TreeFamiTF300367.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.
InterProiIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFiPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEiPS50180. GAE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O43747) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV
60 70 80 90 100
AKLLYMHMLG YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH
110 120 130 140 150
LLMTNCIKND LNHSTQFVQG LALCTLGCMG SSEMCRDLAG EVEKLLKTSN
160 170 180 190 200
SYLRKKAALC AVHVIRKVPE LMEMFLPATK NLLNEKNHGV LHTSVVLLTE
210 220 230 240 250
MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI SDPFLQVRIL
260 270 280 290 300
RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK
310 320 330 340 350
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI
360 370 380 390 400
VDCLKDLDVS IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC
410 420 430 440 450
ASGIFLAAEK YAPSKRWHID TIMRVLTTAG SYVRDDAVPN LIQLITNSVE
460 470 480 490 500
MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI GEYGDLLVSG QCEEEEPIQV
510 520 530 540 550
TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT VNRIKKVVSI
560 570 580 590 600
YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPTEIVQ
610 620 630 640 650
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPS
660 670 680 690 700
SAGGELLDLL GDINLTGAPA AAPAPASVPQ ISQPPFLLDG LSSQPLFNDI
710 720 730 740 750
AAGIPSITAY SKNGLKIEFT FERSNTNPSV TVITIQASNS TELDMTDFVF
760 770 780 790 800
QAAVPKTFQL QLLSPSSSIV PAFNTGTITQ VIKVLNPQKQ QLRMRIKLTY
810 820
NHKGSAMQDL AEVNNFPPQS WQ
Length:822
Mass (Da):91,351
Last modified:May 1, 2007 - v5
Checksum:i42EF40223DFBA5E9
GO
Isoform 2 (identifier: O43747-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     213-213: R → RKNE

Show »
Length:825
Mass (Da):91,723
Checksum:i1F5EA713F104978C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611Y → N in CAA11832. (PubMed:9653655)Curated
Sequence conflicti141 – 1411E → K in CAA11832. (PubMed:9653655)Curated
Sequence conflicti154 – 1541R → K in CAA11832. (PubMed:9653655)Curated
Sequence conflicti174 – 1752MF → NV in CAA11832. (PubMed:9653655)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti195 – 1951V → G.
Corresponds to variant rs36037071 [ dbSNP | Ensembl ].
VAR_048194
Natural varianti685 – 6851P → H.1 Publication
Corresponds to variant rs904763 [ dbSNP | Ensembl ].
VAR_013572

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei213 – 2131R → RKNE in isoform 2. 1 PublicationVSP_040133

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12226 mRNA. Translation: CAA72902.1.
AJ224112, AJ224113, AJ224114 Genomic DNA. Translation: CAA11832.1.
AB015317 mRNA. Translation: BAA33389.1.
AC009097 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
AL050025 mRNA. No translation available.
AL110198 mRNA. Translation: CAB53673.1.
CCDSiCCDS32480.1. [O43747-1]
CCDS45522.1. [O43747-2]
RefSeqiNP_001025178.1. NM_001030007.1. [O43747-2]
NP_001119.3. NM_001128.5. [O43747-1]
UniGeneiHs.461253.

Genome annotation databases

EnsembliENST00000299980; ENSP00000299980; ENSG00000166747. [O43747-1]
ENST00000393512; ENSP00000377148; ENSG00000166747. [O43747-2]
ENST00000569748; ENSP00000454523; ENSG00000166747. [O43747-1]
GeneIDi164.
KEGGihsa:164.
UCSCiuc010cgg.3. human. [O43747-1]
uc021tky.1. human. [O43747-2]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y12226 mRNA. Translation: CAA72902.1 .
AJ224112 , AJ224113 , AJ224114 Genomic DNA. Translation: CAA11832.1 .
AB015317 mRNA. Translation: BAA33389.1 .
AC009097 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
AL050025 mRNA. No translation available.
AL110198 mRNA. Translation: CAB53673.1 .
CCDSi CCDS32480.1. [O43747-1 ]
CCDS45522.1. [O43747-2 ]
RefSeqi NP_001025178.1. NM_001030007.1. [O43747-2 ]
NP_001119.3. NM_001128.5. [O43747-1 ]
UniGenei Hs.461253.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1IU1 X-ray 1.80 A/B 677-822 [» ]
ProteinModelPortali O43747.
SMRi O43747. Positions 1-589, 700-822.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 106673. 31 interactions.
IntActi O43747. 20 interactions.
MINTi MINT-3369703.
STRINGi 9606.ENSP00000377148.

PTM databases

PhosphoSitei O43747.

Proteomic databases

MaxQBi O43747.
PaxDbi O43747.
PRIDEi O43747.

Protocols and materials databases

DNASUi 164.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000299980 ; ENSP00000299980 ; ENSG00000166747 . [O43747-1 ]
ENST00000393512 ; ENSP00000377148 ; ENSG00000166747 . [O43747-2 ]
ENST00000569748 ; ENSP00000454523 ; ENSG00000166747 . [O43747-1 ]
GeneIDi 164.
KEGGi hsa:164.
UCSCi uc010cgg.3. human. [O43747-1 ]
uc021tky.1. human. [O43747-2 ]

Organism-specific databases

CTDi 164.
GeneCardsi GC16M071762.
HGNCi HGNC:555. AP1G1.
HPAi CAB009049.
HPA041224.
MIMi 603533. gene.
neXtProti NX_O43747.
PharmGKBi PA24845.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG303101.
GeneTreei ENSGT00390000012618.
HOGENOMi HOG000210271.
HOVERGENi HBG067473.
InParanoidi O43747.
KOi K12391.
OrthoDBi EOG7S7SD7.
PhylomeDBi O43747.
TreeFami TF300367.

Enzyme and pathway databases

Reactomei REACT_11103. Nef mediated downregulation of MHC class I complex cell surface expression.
REACT_121399. MHC class II antigen presentation.
REACT_19287. Lysosome Vesicle Biogenesis.
REACT_19400. Golgi Associated Vesicle Biogenesis.

Miscellaneous databases

ChiTaRSi AP1G1. human.
EvolutionaryTracei O43747.
GeneWikii AP1G1.
GenomeRNAii 164.
NextBioi 659.
PMAP-CutDB O43747.
PROi O43747.
SOURCEi Search...

Gene expression databases

Bgeei O43747.
CleanExi HS_AP1G1.
ExpressionAtlasi O43747. baseline and differential.
Genevestigatori O43747.

Family and domain databases

Gene3Di 1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.
InterProi IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view ]
Pfami PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view ]
PIRSFi PIRSF037094. AP1_complex_gamma. 1 hit.
SMARTi SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEi PS50180. GAE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG)."
    Peyrard M., Parveneh S., Lagerkrantz S., Ekman M., Fransson I., Sahlen S., Dumanski J.P.
    Genomics 50:275-280(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
  2. "Identification and characterization of novel clathrin adaptor-related proteins."
    Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K.
    J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT HIS-685.
  3. "The sequence and analysis of duplication-rich human chromosome 16."
    Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J.
    , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
    Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-822.
    Tissue: Fetal brain and Uterus.
  5. "Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes."
    Deneka M., Neeft M., Popa I., van Oort M., Sprong H., Oorschot V., Klumperman J., Schu P., van der Sluijs P.
    EMBO J. 22:2645-2657(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RABEP1, SUBCELLULAR LOCATION.
  6. "Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking."
    Neubrand V.E., Will R.D., Moebius W., Poustka A., Wiemann S., Schu P., Dotti C.G., Pepperkok R., Simpson J.C.
    EMBO J. 24:1122-1133(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH AP1AR.
  7. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain."
    Nogi T., Shiba Y., Kawasaki M., Shiba T., Matsugaki N., Igarashi N., Suzuki M., Kato R., Takatsu H., Nakayama K., Wakatsuki S.
    Nat. Struct. Biol. 9:527-531(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 703-822, INTERACTION WITH RABPT5 AND SYNRG.

Entry informationi

Entry nameiAP1G1_HUMAN
AccessioniPrimary (citable) accession number: O43747
Secondary accession number(s): O75709
, O75842, Q9UG09, Q9Y3U4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: October 29, 2014
This is version 143 of the entry and version 5 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3