Reviewed, UniProtKB/Swiss-Prot O43747 (AP1G1_HUMAN)

Names and origin

Protein names

Recommended name:
    AP-1 complex subunit gamma-1
Alternative name(s):
    Adapter-related protein complex 1 subunit gamma-1
    Adaptor protein complex AP-1 subunit gamma-1
    Golgi adaptor HA1/AP1 adaptin subunit gamma-1
    Gamma1-adaptin
    Clathrin assembly protein complex 1 gamma-1 large chain

Gene names

Name:	AP1G1
Synonyms:	ADTG, CLAPG1

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and AP1GBP1.
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note= Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the adaptor complexes large subunit family. Contains 1 GAE domain.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Polymorphism
Technical term	3D-structure
Gene Ontology (GO)
Biological process	endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Ref.2 Traceable author statement. Source: ProtInc
Cellular component	Golgi apparatus Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin-coated vesicle Inferred from direct assay. Source: MGI cytosol Inferred from Experiment. Source: Reactome
Molecular function	protein binding Inferred from electronic annotation. Source: InterPro protein transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 822

821

AP-1 complex subunit gamma-1

PRO_0000193758

Regions

Domain

702 – 817

116

GAE

Natural variations

Natural variant

685

1

P → H: dbSNP rs904763.

VAR_013572

Experimental info

Sequence conflict

61

1

Y → N in CAA11832. Ref.1

Sequence conflict

141

1

E → K in CAA11832. Ref.1

Sequence conflict

154

1

R → K in CAA11832. Ref.1

Sequence conflict

174 – 175

2

MF → NV in CAA11832. Ref.1

Sequence conflict

213

1

R → RENE in AL050025. Ref.4

Sequence conflict

214

1

K → KNEK in CAA72902. Ref.1

Sequence conflict

234

1

E → G in AL050025. Ref.4

Secondary structure

1

.

.

.

.

.

.

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.

.

.

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.

.

.

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822

Helix Strand Turn

Details...

Beta strand

707 – 712

6

Beta strand

715 – 723

9

Beta strand

730 – 739

10

Beta strand

741 – 743

3

Beta strand

745 – 753

9

Beta strand

758 – 762

5

Helix

772 – 774

3

Beta strand

778 – 785

8

Beta strand

795 – 802

8

Beta strand

805 – 812

8

Helix

818 – 820

3

Sequences

	Sequence		Length	Mass (Da)
	O43747-1 [UniParc]. Last modified May 1, 2007. Version 5. Checksum: 42EF40223DFBA5E9	FASTA	822	91,351
	10 20 30 40 50 60 MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG 70 80 90 100 110 120 YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG 130 140 150 160 170 180 LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK 190 200 210 220 230 240 NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI 250 260 270 280 290 300 SDPFLQVRIL RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK 310 320 330 340 350 360 SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI VDCLKDLDVS 370 380 390 400 410 420 IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC ASGIFLAAEK YAPSKRWHID 430 440 450 460 470 480 TIMRVLTTAG SYVRDDAVPN LIQLITNSVE MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI 490 500 510 520 530 540 GEYGDLLVSG QCEEEEPIQV TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT 550 560 570 580 590 600 VNRIKKVVSI YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPTEIVQ 610 620 630 640 650 660 TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPS SAGGELLDLL 670 680 690 700 710 720 GDINLTGAPA AAPAPASVPQ ISQPPFLLDG LSSQPLFNDI AAGIPSITAY SKNGLKIEFT 730 740 750 760 770 780 FERSNTNPSV TVITIQASNS TELDMTDFVF QAAVPKTFQL QLLSPSSSIV PAFNTGTITQ 790 800 810 820 VIKVLNPQKQ QLRMRIKLTY NHKGSAMQDL AEVNNFPPQS WQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG)." Peyrard M., Parveneh S., Lagerkrantz S., Ekman M., Fransson I., Sahlen S., Dumanski J.P. Genomics 50:275-280(1998) [PubMed: 9653655] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[2]	"Identification and characterization of novel clathrin adaptor-related proteins." Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K. J. Biol. Chem. 273:24693-24700(1998) [PubMed: 9733768] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], VARIANT HIS-685.
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed: 15616553] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	The German cDNA consortium Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 174-822. Tissue: Fetal brain and Uterus.
[5]	"Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes." Deneka M., Neeft M., Popa I., van Oort M., Sprong H., Oorschot V., Klumperman J., Schu P., van der Sluijs P. EMBO J. 22:2645-2657(2003) [PubMed: 12773381] [Abstract] Cited for: INTERACTION WITH RABEP1, SUBCELLULAR LOCATION.
[6]	"Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain." Nogi T., Shiba Y., Kawasaki M., Shiba T., Matsugaki N., Igarashi N., Suzuki M., Kato R., Takatsu H., Nakayama K., Wakatsuki S. Nat. Struct. Biol. 9:527-531(2002) [PubMed: 12042876] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 703-822, INTERACTION WITH RABPT5 AND AP1GBP1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

Y12226 mRNA. Translation: CAA72902.1.
AJ224112, AJ224113, AJ224114 Genomic DNA. Translation: CAA11832.1.
AB015317 mRNA. Translation: BAA33389.1.
AC009097 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
AL050025 mRNA. No translation available.
AL110198 mRNA. Translation: CAB53673.1.

RefSeq

NP_001119.3.

UniGene

Hs.461253

3D structure databases

PDBMSD

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IU1	X-ray	1.80	A/B	677-822	[»]

SMR

O43747. Positions 1-589.

ModBase

Search...

Protein-protein interaction databases

IntAct

O43747.

PTM databases

PhosphoSite

O43747.

Genome annotation databases

Ensembl

ENSG00000166747. Homo sapiens. [Contig view]

GeneID

164.

Organism-specific databases

HGNC

HGNC:555. AP1G1.

HPA

CAB009049.

MIM

603533. gene.

PharmGKB

PA24845.

GenAtlas

Search...

GeneCards

Search...

Phylogenomic databases

HOVERGEN

O43747.

Enzyme and pathway databases

Reactome

REACT_6185. HIV Infection.

Gene expression databases

ArrayExpress

O43747.

CleanEx

HS_AP1G1.

GermOnline

ENSG00000166747. Homo sapiens.

Family and domain databases

InterPro

IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
[Graphical view]

Gene3D

G3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1230. Clathrin_g-adaptin_app. 1 hit.

Pfam

PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]

PIRSF

PIRSF037094. AP1_complex_gamma. 1 hit.

ProDom

PD021457. Gamma_adaptin_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]

SMART

SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]

PROSITE

PS50180. GAE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other Resources

NextBio

659.

SOURCE

Search...

Entry information

Entry name

AP1G1_HUMAN

Accession

Primary (citable) accession number: O43747
Secondary accession number(s): O75709 , O75842, Q9UG09, Q9Y3U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 2007
Last modified:	November 25, 2008
This is version 86 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)