O43747 (AP1G1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
AP-1 complex subunit gamma-1

Alternative name(s):
Adapter-related protein complex 1 subunit gamma-1
Adaptor protein complex AP-1 subunit gamma-1
Clathrin assembly protein complex 1 gamma-1 large chain
Gamma1-adaptin
Golgi adaptor HA1/AP1 adaptin subunit gamma-1

Gene names

Name:	AP1G1
Synonyms:	ADTG, CLAPG1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	822 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.
Subunit structure	Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain). Ref.5 Ref.6 Ref.8
Subcellular location	Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. Ref.5
Tissue specificity	Widely expressed.
Sequence similarities	Belongs to the adaptor complexes large subunit family. Contains 1 GAE domain.

Ontologies

Keywords
Biological process	Endocytosis Protein transport Transport
Cellular component	Cytoplasmic vesicle Golgi apparatus Membrane
Coding sequence diversity	Alternative splicing Polymorphism
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	antigen processing and presentation of exogenous peptide antigen via MHC class II Traceable author statement. Source: Reactome cellular membrane organization Traceable author statement. Source: Reactome endocytosis Inferred from electronic annotation. Source: UniProtKB-KW intracellular protein transport Traceable author statement Ref.2. Source: ProtInc post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome regulation of defense response to virus by virus Traceable author statement. Source: Reactome viral reproduction Traceable author statement. Source: Reactome
Cellular_component	AP-type membrane coat adaptor complex Traceable author statement Ref.1. Source: ProtInc Golgi membrane Traceable author statement. Source: Reactome clathrin adaptor complex Inferred from electronic annotation. Source: InterPro clathrin-coated vesicle membrane Inferred from sequence or structural similarity. Source: BHF-UCL cytosol Traceable author statement. Source: Reactome lysosomal membrane Traceable author statement. Source: Reactome perinuclear region of cytoplasm Inferred from electronic annotation. Source: Compara recycling endosome Inferred from direct assay PubMed 19841138. Source: UniProtKB trans-Golgi network membrane Traceable author statement. Source: Reactome
Molecular_function	protein transporter activity Inferred from electronic annotation. Source: InterPro transporter activity Traceable author statement Ref.1Ref.2. Source: ProtInc
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
RABEP1	Q15276	2	EBI-447609,EBI-447043

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43747-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43747-2)

The sequence of this isoform differs from the canonical sequence as follows:
     213-213: R → RKNE

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 822

821

AP-1 complex subunit gamma-1

PRO_0000193758

Regions

Domain

702 – 817

116

GAE

Natural variations

Alternative sequence

213

1

R → RKNE in isoform 2.

VSP_040133

Natural variant

195

1

V → G.
Corresponds to variant rs36037071 [ dbSNP | Ensembl ].

VAR_048194

Natural variant

685

1

P → H. Ref.2
Corresponds to variant rs904763 [ dbSNP | Ensembl ].

VAR_013572

Experimental info

Sequence conflict

61

1

Y → N in CAA11832. Ref.1

Sequence conflict

141

1

E → K in CAA11832. Ref.1

Sequence conflict

154

1

R → K in CAA11832. Ref.1

Sequence conflict

174 – 175

2

MF → NV in CAA11832. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

822

Helix Strand Turn

Details...

Beta strand

707 – 712

6

Beta strand

715 – 723

9

Beta strand

730 – 739

10

Beta strand

741 – 743

3

Beta strand

745 – 753

9

Beta strand

758 – 762

5

Helix

772 – 774

3

Beta strand

778 – 785

8

Beta strand

795 – 802

8

Beta strand

805 – 812

8

Helix

818 – 820

3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 1, 2007. Version 5. Checksum: 42EF40223DFBA5E9	FASTA	822	91,351
	10 20 30 40 50 60 MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG 70 80 90 100 110 120 YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG 130 140 150 160 170 180 LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK 190 200 210 220 230 240 NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI 250 260 270 280 290 300 SDPFLQVRIL RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK 310 320 330 340 350 360 SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI VDCLKDLDVS 370 380 390 400 410 420 IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC ASGIFLAAEK YAPSKRWHID 430 440 450 460 470 480 TIMRVLTTAG SYVRDDAVPN LIQLITNSVE MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI 490 500 510 520 530 540 GEYGDLLVSG QCEEEEPIQV TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT 550 560 570 580 590 600 VNRIKKVVSI YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPTEIVQ 610 620 630 640 650 660 TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPS SAGGELLDLL 670 680 690 700 710 720 GDINLTGAPA AAPAPASVPQ ISQPPFLLDG LSSQPLFNDI AAGIPSITAY SKNGLKIEFT 730 740 750 760 770 780 FERSNTNPSV TVITIQASNS TELDMTDFVF QAAVPKTFQL QLLSPSSSIV PAFNTGTITQ 790 800 810 820 VIKVLNPQKQ QLRMRIKLTY NHKGSAMQDL AEVNNFPPQS WQ
	Isoform 2 [UniParc]. Checksum: 1F5EA713F104978C	FASTA	825	91,723
	10 20 30 40 50 60 MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG 70 80 90 100 110 120 YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG 130 140 150 160 170 180 LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK 190 200 210 220 230 240 NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKNEKLVP QLVRILKNLI MSGYSPEHDV 250 260 270 280 290 300 SGISDPFLQV RILRLLRILG RNDDDSSEAM NDILAQVATN TETSKNVGNA ILYETVLTIM 310 320 330 340 350 360 DIKSESGLRV LAINILGRFL LNNDKNIRYV ALTSLLKTVQ TDHNAVQRHR STIVDCLKDL 370 380 390 400 410 420 DVSIKRRAME LSFALVNGNN IRGMMKELLY FLDSCEPEFK ADCASGIFLA AEKYAPSKRW 430 440 450 460 470 480 HIDTIMRVLT TAGSYVRDDA VPNLIQLITN SVEMHAYTVQ RLYKAILGDY SQQPLVQVAA 490 500 510 520 530 540 WCIGEYGDLL VSGQCEEEEP IQVTEDEVLD ILESVLISNM STSVTRGYAL TAIMKLSTRF 550 560 570 580 590 600 TCTVNRIKKV VSIYGSSIDV ELQQRAVEYN ALFKKYDHMR SALLERMPVM EKVTTNGPTE 610 620 630 640 650 660 IVQTNGETEP APLETKPPPS GPQPTSQAND LLDLLGGNDI TPVIPTAPTS KPSSAGGELL 670 680 690 700 710 720 DLLGDINLTG APAAAPAPAS VPQISQPPFL LDGLSSQPLF NDIAAGIPSI TAYSKNGLKI 730 740 750 760 770 780 EFTFERSNTN PSVTVITIQA SNSTELDMTD FVFQAAVPKT FQLQLLSPSS SIVPAFNTGT 790 800 810 820 ITQVIKVLNP QKQQLRMRIK LTYNHKGSAM QDLAEVNNFP PQSWQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG)." Peyrard M., Parveneh S., Lagerkrantz S., Ekman M., Fransson I., Sahlen S., Dumanski J.P. Genomics 50:275-280(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
[2]	"Identification and characterization of novel clathrin adaptor-related proteins." Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K. J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT HIS-685.
[3]	"The sequence and analysis of duplication-rich human chromosome 16." Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A. Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-822. Tissue: Fetal brain and Uterus.
[5]	"Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes." Deneka M., Neeft M., Popa I., van Oort M., Sprong H., Oorschot V., Klumperman J., Schu P., van der Sluijs P. EMBO J. 22:2645-2657(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH RABEP1, SUBCELLULAR LOCATION.
[6]	"Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking." Neubrand V.E., Will R.D., Moebius W., Poustka A., Wiemann S., Schu P., Dotti C.G., Pepperkok R., Simpson J.C. EMBO J. 24:1122-1133(2005) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH AP1AR.
[7]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]	"Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain." Nogi T., Shiba Y., Kawasaki M., Shiba T., Matsugaki N., Igarashi N., Suzuki M., Kato R., Takatsu H., Nakayama K., Wakatsuki S. Nat. Struct. Biol. 9:527-531(2002) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 703-822, INTERACTION WITH RABPT5 AND SYNRG.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Y12226 mRNA. Translation: CAA72902.1.
AJ224112, AJ224113, AJ224114 Genomic DNA. Translation: CAA11832.1.
AB015317 mRNA. Translation: BAA33389.1.
AC009097 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
AL050025 mRNA. No translation available.
AL110198 mRNA. Translation: CAB53673.1.

IPI

IPI00293396.
IPI00939879.

RefSeq

NP_001025178.1. NM_001030007.1.
NP_001119.3. NM_001128.5.

UniGene

Hs.461253.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1IU1	X-ray	1.80	A/B	677-822	[»]

ProteinModelPortal

O43747.

ModBase

Search...

Protein-protein interaction databases

IntAct

O43747. 15 interactions.

MINT

MINT-3369703.

STRING

9606.ENSP00000377148.

PTM databases

PhosphoSite

O43747.

Proteomic databases

PaxDb

O43747.

PRIDE

O43747.

Protocols and materials databases

DNASU

164.

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000299980; ENSP00000299980; ENSG00000166747.
ENST00000393512; ENSP00000377148; ENSG00000166747.
ENST00000569748; ENSP00000454523; ENSG00000166747.

GeneID

164.

KEGG

hsa:164.

UCSC

uc010cgg.3. human.
uc021tky.1. human.

Organism-specific databases

CTD

164.

GeneCards

GC16M071762.

HGNC

HGNC:555. AP1G1.

HPA

CAB009049.

MIM

603533. gene.

neXtProt

NX_O43747.

PharmGKB

PA24845.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG303101.

HOGENOM

HOG000210271.

HOVERGEN

HBG067473.

KO

K12391.

OrthoDB

EOG4ZGPBP.

Enzyme and pathway databases

Reactome

REACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpress

O43747.

Bgee

O43747.

CleanEx

HS_AP1G1.

Genevestigator

O43747.

GermOnline

ENSG00000166747. Homo sapiens.

Family and domain databases

Gene3D

1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.

InterPro

IPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]

PANTHER

PTHR22780:SF5. PTHR22780:SF5. 1 hit.

Pfam

PF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]

PIRSF

PIRSF037094. AP1_complex_gamma. 1 hit.

SMART

SM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]

SUPFAM

SSF48371. ARM-type_fold. 1 hit.
SSF49348. Clath_adapt. 1 hit.

PROSITE

PS50180. GAE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

AP1G1. human.

EvolutionaryTrace

O43747.

GenomeRNAi

164.

NextBio

659.

PMAP-CutDB

O43747.

SOURCE

Search...

Entry information

Entry name

AP1G1_HUMAN

Accession

Primary (citable) accession number: O43747
Secondary accession number(s): O75709 , O75842, Q9UG09, Q9Y3U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	May 1, 2007
Last modified:	May 1, 2013
This is version 130 of the entry and version 5 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.