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O43747 (AP1G1_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 137. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (6) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit gamma-1
Alternative name(s):
Adapter-related protein complex 1 subunit gamma-1
Adaptor protein complex AP-1 subunit gamma-1
Clathrin assembly protein complex 1 gamma-1 large chain
Gamma1-adaptin
Golgi adaptor HA1/AP1 adaptin subunit gamma-1
Gene names
Name:AP1G1
Synonyms:ADTG, CLAPG1
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length822 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Binds RABEP1 and SYNRG. Interacts (via GAE domain) with AP1AR (via coiled-coil domain). Ref.5 Ref.6 Ref.8

Subcellular location

Golgi apparatus. Cytoplasmic vesicleclathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex. Ref.5

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Contains 1 GAE domain.

Ontologies

Keywords
   Biological processProtein transport
Transport
   Cellular componentCytoplasmic vesicle
Golgi apparatus
Membrane
   Coding sequence diversityAlternative splicing
Polymorphism
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processGolgi to lysosome transport

Inferred from mutant phenotype PubMed 23632890. Source: UniProt

antigen processing and presentation of exogenous peptide antigen via MHC class II

Traceable author statement. Source: Reactome

intracellular protein transport

Traceable author statement Ref.2. Source: ProtInc

membrane organization

Traceable author statement. Source: Reactome

positive regulation of natural killer cell degranulation

Inferred from mutant phenotype PubMed 23632890. Source: UniProt

positive regulation of natural killer cell mediated cytotoxicity

Inferred from mutant phenotype PubMed 23632890. Source: UniProt

post-Golgi vesicle-mediated transport

Traceable author statement. Source: Reactome

regulation of defense response to virus by virus

Traceable author statement. Source: Reactome

viral process

Traceable author statement. Source: Reactome

   Cellular_componentAP-type membrane coat adaptor complex

Traceable author statement Ref.1. Source: ProtInc

Golgi apparatus

Inferred from direct assay Ref.2. Source: UniProtKB

Golgi membrane

Traceable author statement. Source: Reactome

clathrin adaptor complex

Inferred from electronic annotation. Source: InterPro

clathrin-coated vesicle

Inferred from direct assay PubMed 12536145. Source: MGI

clathrin-coated vesicle membrane

Inferred from sequence or structural similarity. Source: BHF-UCL

cytoplasm

Inferred from direct assay. Source: HPA

cytoplasmic vesicle membrane

Traceable author statement. Source: Reactome

cytosol

Traceable author statement. Source: Reactome

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

lysosomal membrane

Traceable author statement. Source: Reactome

perinuclear region of cytoplasm

Inferred from electronic annotation. Source: Ensembl

recycling endosome

Inferred from direct assay PubMed 19841138. Source: UniProtKB

trans-Golgi network membrane

Traceable author statement. Source: Reactome

   Molecular_functionkinesin binding

Inferred from physical interaction PubMed 19841138. Source: UniProtKB

protein transporter activity

Inferred from electronic annotation. Source: InterPro

transporter activity

Traceable author statement Ref.1Ref.2. Source: ProtInc

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RABEP1Q152762EBI-447609,EBI-447043

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O43747-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O43747-2)

The sequence of this isoform differs from the canonical sequence as follows:
     213-213: R → RKNE

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 822821AP-1 complex subunit gamma-1
PRO_0000193758

Regions

Domain702 – 817116GAE

Natural variations

Alternative sequence2131R → RKNE in isoform 2.
VSP_040133
Natural variant1951V → G.
Corresponds to variant rs36037071 [ dbSNP | Ensembl ].
VAR_048194
Natural variant6851P → H. Ref.2
Corresponds to variant rs904763 [ dbSNP | Ensembl ].
VAR_013572

Experimental info

Sequence conflict611Y → N in CAA11832. Ref.1
Sequence conflict1411E → K in CAA11832. Ref.1
Sequence conflict1541R → K in CAA11832. Ref.1
Sequence conflict174 – 1752MF → NV in CAA11832. Ref.1

Secondary structure

....................... 822
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified May 1, 2007. Version 5.
Checksum: 42EF40223DFBA5E9

FASTA82291,351
        10         20         30         40         50         60 
MPAPIRLREL IRTIRTARTQ AEEREMIQKE CAAIRSSFRE EDNTYRCRNV AKLLYMHMLG 

        70         80         90        100        110        120 
YPAHFGQLEC LKLIASQKFT DKRIGYLGAM LLLDERQDVH LLMTNCIKND LNHSTQFVQG 

       130        140        150        160        170        180 
LALCTLGCMG SSEMCRDLAG EVEKLLKTSN SYLRKKAALC AVHVIRKVPE LMEMFLPATK 

       190        200        210        220        230        240 
NLLNEKNHGV LHTSVVLLTE MCERSPDMLA HFRKLVPQLV RILKNLIMSG YSPEHDVSGI 

       250        260        270        280        290        300 
SDPFLQVRIL RLLRILGRND DDSSEAMNDI LAQVATNTET SKNVGNAILY ETVLTIMDIK 

       310        320        330        340        350        360 
SESGLRVLAI NILGRFLLNN DKNIRYVALT SLLKTVQTDH NAVQRHRSTI VDCLKDLDVS 

       370        380        390        400        410        420 
IKRRAMELSF ALVNGNNIRG MMKELLYFLD SCEPEFKADC ASGIFLAAEK YAPSKRWHID 

       430        440        450        460        470        480 
TIMRVLTTAG SYVRDDAVPN LIQLITNSVE MHAYTVQRLY KAILGDYSQQ PLVQVAAWCI 

       490        500        510        520        530        540 
GEYGDLLVSG QCEEEEPIQV TEDEVLDILE SVLISNMSTS VTRGYALTAI MKLSTRFTCT 

       550        560        570        580        590        600 
VNRIKKVVSI YGSSIDVELQ QRAVEYNALF KKYDHMRSAL LERMPVMEKV TTNGPTEIVQ 

       610        620        630        640        650        660 
TNGETEPAPL ETKPPPSGPQ PTSQANDLLD LLGGNDITPV IPTAPTSKPS SAGGELLDLL 

       670        680        690        700        710        720 
GDINLTGAPA AAPAPASVPQ ISQPPFLLDG LSSQPLFNDI AAGIPSITAY SKNGLKIEFT 

       730        740        750        760        770        780 
FERSNTNPSV TVITIQASNS TELDMTDFVF QAAVPKTFQL QLLSPSSSIV PAFNTGTITQ 

       790        800        810        820 
VIKVLNPQKQ QLRMRIKLTY NHKGSAMQDL AEVNNFPPQS WQ 

« Hide

Isoform 2 [UniParc].

Checksum: 1F5EA713F104978C
Show »

FASTA82591,723

References

« Hide 'large scale' references
[1]"Cloning, expression pattern, and chromosomal assignment to 16q23 of the human gamma-adaptin gene (ADTG)."
Peyrard M., Parveneh S., Lagerkrantz S., Ekman M., Fransson I., Sahlen S., Dumanski J.P.
Genomics 50:275-280(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 2).
[2]"Identification and characterization of novel clathrin adaptor-related proteins."
Takatsu H., Sakurai M., Shin H.-W., Murakami K., Nakayama K.
J. Biol. Chem. 273:24693-24700(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT HIS-685.
[3]"The sequence and analysis of duplication-rich human chromosome 16."
Martin J., Han C., Gordon L.A., Terry A., Prabhakar S., She X., Xie G., Hellsten U., Chan Y.M., Altherr M., Couronne O., Aerts A., Bajorek E., Black S., Blumer H., Branscomb E., Brown N.C., Bruno W.J. expand/collapse author list , Buckingham J.M., Callen D.F., Campbell C.S., Campbell M.L., Campbell E.W., Caoile C., Challacombe J.F., Chasteen L.A., Chertkov O., Chi H.C., Christensen M., Clark L.M., Cohn J.D., Denys M., Detter J.C., Dickson M., Dimitrijevic-Bussod M., Escobar J., Fawcett J.J., Flowers D., Fotopulos D., Glavina T., Gomez M., Gonzales E., Goodstein D., Goodwin L.A., Grady D.L., Grigoriev I., Groza M., Hammon N., Hawkins T., Haydu L., Hildebrand C.E., Huang W., Israni S., Jett J., Jewett P.B., Kadner K., Kimball H., Kobayashi A., Krawczyk M.-C., Leyba T., Longmire J.L., Lopez F., Lou Y., Lowry S., Ludeman T., Manohar C.F., Mark G.A., McMurray K.L., Meincke L.J., Morgan J., Moyzis R.K., Mundt M.O., Munk A.C., Nandkeshwar R.D., Pitluck S., Pollard M., Predki P., Parson-Quintana B., Ramirez L., Rash S., Retterer J., Ricke D.O., Robinson D.L., Rodriguez A., Salamov A., Saunders E.H., Scott D., Shough T., Stallings R.L., Stalvey M., Sutherland R.D., Tapia R., Tesmer J.G., Thayer N., Thompson L.S., Tice H., Torney D.C., Tran-Gyamfi M., Tsai M., Ulanovsky L.E., Ustaszewska A., Vo N., White P.S., Williams A.L., Wills P.L., Wu J.-R., Wu K., Yang J., DeJong P., Bruce D., Doggett N.A., Deaven L., Schmutz J., Grimwood J., Richardson P., Rokhsar D.S., Eichler E.E., Gilna P., Lucas S.M., Myers R.M., Rubin E.M., Pennacchio L.A.
Nature 432:988-994(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]"The full-ORF clone resource of the German cDNA consortium."
Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I.
BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 539-822.
Tissue: Fetal brain and Uterus.
[5]"Rabaptin-5alpha/rabaptin-4 serves as a linker between rab4 and gamma(1)-adaptin in membrane recycling from endosomes."
Deneka M., Neeft M., Popa I., van Oort M., Sprong H., Oorschot V., Klumperman J., Schu P., van der Sluijs P.
EMBO J. 22:2645-2657(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RABEP1, SUBCELLULAR LOCATION.
[6]"Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking."
Neubrand V.E., Will R.D., Moebius W., Poustka A., Wiemann S., Schu P., Dotti C.G., Pepperkok R., Simpson J.C.
EMBO J. 24:1122-1133(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH AP1AR.
[7]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"Structural basis for the accessory protein recruitment by the gamma-adaptin ear domain."
Nogi T., Shiba Y., Kawasaki M., Shiba T., Matsugaki N., Igarashi N., Suzuki M., Kato R., Takatsu H., Nakayama K., Wakatsuki S.
Nat. Struct. Biol. 9:527-531(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 703-822, INTERACTION WITH RABPT5 AND SYNRG.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Y12226 mRNA. Translation: CAA72902.1.
AJ224112, AJ224113, AJ224114 Genomic DNA. Translation: CAA11832.1.
AB015317 mRNA. Translation: BAA33389.1.
AC009097 Genomic DNA. No translation available.
AC010653 Genomic DNA. No translation available.
AL050025 mRNA. No translation available.
AL110198 mRNA. Translation: CAB53673.1.
RefSeqNP_001025178.1. NM_001030007.1.
NP_001119.3. NM_001128.5.
UniGeneHs.461253.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1IU1X-ray1.80A/B677-822[»]
ProteinModelPortalO43747.
SMRO43747. Positions 1-589, 700-822.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid106673. 27 interactions.
IntActO43747. 20 interactions.
MINTMINT-3369703.
STRING9606.ENSP00000377148.

PTM databases

PhosphoSiteO43747.

Proteomic databases

PaxDbO43747.
PRIDEO43747.

Protocols and materials databases

DNASU164.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000299980; ENSP00000299980; ENSG00000166747. [O43747-1]
ENST00000393512; ENSP00000377148; ENSG00000166747. [O43747-2]
ENST00000569748; ENSP00000454523; ENSG00000166747. [O43747-1]
GeneID164.
KEGGhsa:164.
UCSCuc010cgg.3. human. [O43747-1]
uc021tky.1. human. [O43747-2]

Organism-specific databases

CTD164.
GeneCardsGC16M071762.
HGNCHGNC:555. AP1G1.
HPACAB009049.
HPA041224.
MIM603533. gene.
neXtProtNX_O43747.
PharmGKBPA24845.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG303101.
HOGENOMHOG000210271.
HOVERGENHBG067473.
KOK12391.
OrthoDBEOG7S7SD7.
PhylomeDBO43747.
TreeFamTF300367.

Enzyme and pathway databases

ReactomeREACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressO43747.
BgeeO43747.
CleanExHS_AP1G1.
GenevestigatorO43747.

Family and domain databases

Gene3D1.25.10.10. 1 hit.
2.60.40.1230. 2 hits.
InterProIPR017107. AP1_complex_gsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR008153. Clathrin_g-adaptin_app.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERPTHR22780:SF5. PTHR22780:SF5. 1 hit.
PfamPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
[Graphical view]
PIRSFPIRSF037094. AP1_complex_gamma. 1 hit.
SMARTSM00809. Alpha_adaptinC2. 1 hit.
[Graphical view]
SUPFAMSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
PROSITEPS50180. GAE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSAP1G1. human.
EvolutionaryTraceO43747.
GeneWikiAP1G1.
GenomeRNAi164.
NextBio659.
PMAP-CutDBO43747.
PROO43747.
SOURCESearch...

Entry information

Entry nameAP1G1_HUMAN
AccessionPrimary (citable) accession number: O43747
Secondary accession number(s): O75709 expand/collapse secondary AC list , O75842, Q9UG09, Q9Y3U4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 137 of the entry and version 5 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 16

Human chromosome 16: entries, gene names and cross-references to MIM