O43745 (CHP2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Calcineurin B homologous protein 2

Alternative name(s):
Hepatocellular carcinoma-associated antigen 520

Gene names

Name:	CHP2
Synonyms:	HCA520

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	196 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Ref.3
Subunit structure	Binds to SLC9A1/NHE1. Ref.3
Tissue specificity	Expressed in malignantly transformed cells but not detected in normal tissues. Ref.2 Ref.3
Sequence similarities	Contains 4 EF-hand domains.

Ontologies

Keywords
Coding sequence diversity	Polymorphism
Domain	Repeat
Ligand	Calcium
PTM	Lipoprotein Myristate
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 196

195

Calcineurin B homologous protein 2

PRO_0000073848

Regions

Domain

26 – 61

36

EF-hand 1

Domain

71 – 106

36

EF-hand 2

Domain

111 – 146

36

EF-hand 3

Domain

152 – 187

36

EF-hand 4

Calcium binding

124 – 135

12

1 Potential

Calcium binding

165 – 176

12

2 Potential

Amino acid modifications

Lipidation

2

1

N-myristoyl glycine Potential

Natural variations

Natural variant

127

1

R → P.
Corresponds to variant rs35641939 [ dbSNP | Ensembl ].

VAR_048664

Secondary structure

1

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196

Helix Strand Turn

Details...

Turn

14 – 16

3

Helix

17 – 21

5

Helix

25 – 38

14

Helix

48 – 52

5

Helix

55 – 59

5

Helix

63 – 68

6

Helix

80 – 87

8

Helix

88 – 90

3

Helix

95 – 98

4

Helix

112 – 123

12

Beta strand

128 – 131

4

Helix

133 – 142

10

Helix

150 – 164

15

Beta strand

169 – 173

5

Helix

174 – 179

6

Turn

180 – 183

4

Helix

186 – 189

4

Turn

193 – 195

3

Sequences

	Sequence		Length	Mass (Da)
	O43745 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: F5113D558AFA27DE	FASTA	196	22,452
	10 20 30 40 50 60 MGSRSSHAAV IPDGDSIRRE TGFSQASLLR LHHRFRALDR NKKGYLSRMD LQQIGALAVN 70 80 90 100 110 120 PLGDRIIESF FPDGSQRVDF PGFVRVLAHF RPVEDEDTET QDPKKPEPLN SRRNKLHYAF 130 140 150 160 170 180 QLYDLDRDGK ISRHEMLQVL RLMVGVQVTE EQLENIADRT VQEADEDGDG AVSFVEFTKS 190 LEKMDVEQKM SIRILK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Genome duplications and other features in 12 Mb of DNA sequence from human chromosome 16p and 16q." Loftus B.J., Kim U.-J., Sneddon V.P., Kalush F., Brandon R., Fuhrmann J., Mason T., Crosby M.L., Barnstead M., Cronin L., Mays A.D., Cao Y., Xu R.X., Kang H.-L., Mitchell S., Eichler E.E., Harris P.C., Venter J.C., Adams M.D. Genomics 60:295-308(1999) [PubMed: 10493829] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[2]	"Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies." Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F. J. Immunol. 169:1102-1109(2002) [PubMed: 12097419] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Tissue: Hepatoma.
[3]	"Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger." Pang T., Wakabayashi S., Shigekawa M. J. Biol. Chem. 277:43771-43777(2002) [PubMed: 12226101] [Abstract] Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SLC9A1.
[4]	"Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation." Ammar Y.B., Takeda S., Hisamitsu T., Mori H., Wakabayashi S. EMBO J. 25:2315-2325(2006) [PubMed: 16710297] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-195 IN COMPLEX WITH SLC9A1.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AC130454 Genomic DNA. No translation available.
AF146019 mRNA. Translation: AAG14945.1.

IPI

IPI00219584.

RefSeq

NP_071380.1. NM_022097.2.

UniGene

Hs.178589.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2BEC	X-ray	2.70	A	1-195	[»]

ProteinModelPortal

O43745.

SMR

O43745. Positions 11-196.

ModBase

Search...

Protein-protein interaction databases

MINT

MINT-2729920.

STRING

O43745.

Proteomic databases

PRIDE

O43745.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000300113; ENSP00000300113; ENSG00000166869.

GeneID

63928.

KEGG

hsa:63928.

UCSC

uc002dmb.1. human.

Organism-specific databases

CTD

63928.

GeneCards

GC16P023765.

H-InvDB

HIX0012897.

neXtProt

NX_O43745.

Phylogenomic databases

eggNOG

prNOG11216.

GeneTree

ENSGT00600000084053.

HOGENOM

HBG746798.

HOVERGEN

HBG105307.

InParanoid

O43745.

OMA

FFPNGSQ.

OrthoDB

EOG4PG61X.

PhylomeDB

O43745.

Gene expression databases

ArrayExpress

O43745.

Bgee

O43745.

Genevestigator

O43745.

GermOnline

ENSG00000166869. Homo sapiens.

Family and domain databases

InterPro

IPR018248. EF-hand.
IPR011992. EF-hand-like_dom.
IPR018247. EF_Hand_1_Ca_BS.
IPR018249. EF_HAND_2.
IPR002048. EF_hand_Ca-bd.
[Graphical view]

Gene3D

G3DSA:1.10.238.10. EF-Hand_type. 1 hit.

KO

K06268.

Pfam

PF00036. efhand. 1 hit.
[Graphical view]

SMART

SM00054. EFh. 3 hits.
[Graphical view]

PROSITE

PS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

ProtoNet

Search...

Other

NextBio

65671.

Entry information

Entry name

CHP2_HUMAN

Accession

Primary (citable) accession number: O43745

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	January 23, 2007
Last modified:	January 25, 2012
This is version 94 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.