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Protein

Calcineurin B homologous protein 2

Gene

CHP2

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na+/H+ exchange activity. Binds to and activates SLC9A1/NHE1 in a serum-independent manner, thus increasing pH and protecting cells from serum deprivation-induced death. Also plays a role in the regulation of cell proliferation and tumor growth by increasing the phosphatase activity of PPP3CA in a calcium-dependent manner. Activator of the calcineurin/NFAT signaling pathway. Involved in the cytoplasmic translocation of the transcription factor NFATC3 to the nucleus.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Calcium bindingi124 – 135121PROSITE-ProRule annotationAdd
BLAST
Calcium bindingi165 – 176122PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • calcium ion binding Source: UniProtKB

GO - Biological processi

  • cellular response to calcium ion Source: UniProtKB
  • positive regulation of calcineurin-NFAT signaling cascade Source: UniProtKB
  • positive regulation of cell proliferation Source: UniProtKB
  • positive regulation of phosphatase activity Source: UniProtKB
  • positive regulation of protein import into nucleus Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Calcineurin B homologous protein 2
Alternative name(s):
Hepatocellular carcinoma-associated antigen 520
Gene namesi
Name:CHP2
Synonyms:HCA520
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 16

Organism-specific databases

HGNCiHGNC:24927. CHP2.

Subcellular locationi

  • Nucleus 1 Publication
  • Cytoplasm 1 Publication
  • Cell membrane 1 Publication

  • Note: Predominantly localized in a juxtanuclear region. Colocalizes with SLC9A3 in the juxtanuclear region and at the plasma membrane (By similarity). Exported from the nucleus to the cytoplasm through a nuclear export signal (NES) pathway. May shuttle between nucleus and cytoplasm.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi50 – 501D → A: Does not reduce calcium-binding. 1 Publication
Mutagenesisi82 – 821G → A: Does not reduce calcium-binding. 1 Publication
Mutagenesisi135 – 1351E → A: Reduces calcium-binding. Inhibits calcium-binding and cell membrane localization; when associated with A-176. 1 Publication
Mutagenesisi137 – 14812LQVLR…VGVQV → AQVARAMAGAQA: Localizes in the nucleus and increases cell proliferation. 1 PublicationAdd
BLAST
Mutagenesisi176 – 1761E → A: Reduces calcium-binding. Inhibits calcium-binding and cell membrane localization; when associated with A-135. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedSequence analysis
Chaini2 – 196195Calcineurin B homologous protein 2PRO_0000073848Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineSequence analysis
Modified residuei27 – 271PhosphoserineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Phosphoprotein

Proteomic databases

PaxDbiO43745.
PRIDEiO43745.

PTM databases

iPTMnetiO43745.

Expressioni

Tissue specificityi

Expressed in malignantly transformed cells but not detected in normal tissues.2 Publications

Gene expression databases

BgeeiO43745.
GenevisibleiO43745. HS.

Organism-specific databases

HPAiCAB072806.

Interactioni

Subunit structurei

Interacts with PPP3CA. Interacts with SLC9A1/NHE1; the interaction occurs in a calcium-dependent manner.4 Publications

Protein-protein interaction databases

BioGridi121996. 5 interactions.
IntActiO43745. 1 interaction.
MINTiMINT-2729920.
STRINGi9606.ENSP00000300113.

Structurei

Secondary structure

1
196
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni14 – 163Combined sources
Helixi17 – 215Combined sources
Helixi25 – 3814Combined sources
Helixi48 – 525Combined sources
Helixi55 – 595Combined sources
Helixi63 – 686Combined sources
Helixi80 – 878Combined sources
Helixi88 – 903Combined sources
Helixi95 – 984Combined sources
Helixi112 – 12312Combined sources
Beta strandi128 – 1314Combined sources
Helixi133 – 14210Combined sources
Helixi150 – 16415Combined sources
Beta strandi169 – 1735Combined sources
Helixi174 – 1796Combined sources
Turni180 – 1834Combined sources
Helixi186 – 1894Combined sources
Turni193 – 1953Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BECX-ray2.70A1-196[»]
ProteinModelPortaliO43745.
SMRiO43745. Positions 11-196.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43745.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 6136EF-hand 1PROSITE-ProRule annotationAdd
BLAST
Domaini71 – 10636EF-hand 2PROSITE-ProRule annotationAdd
BLAST
Domaini111 – 14636EF-hand 3PROSITE-ProRule annotationAdd
BLAST
Domaini152 – 18736EF-hand 4PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi137 – 14812Nuclear export signalAdd
BLAST

Sequence similaritiesi

Contains 4 EF-hand domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
HOGENOMiHOG000233019.
HOVERGENiHBG105307.
InParanoidiO43745.
KOiK17611.
OMAiHFRPVDE.
OrthoDBiEOG78M035.
PhylomeDBiO43745.
TreeFamiTF354284.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43745-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGSRSSHAAV IPDGDSIRRE TGFSQASLLR LHHRFRALDR NKKGYLSRMD
60 70 80 90 100
LQQIGALAVN PLGDRIIESF FPDGSQRVDF PGFVRVLAHF RPVEDEDTET
110 120 130 140 150
QDPKKPEPLN SRRNKLHYAF QLYDLDRDGK ISRHEMLQVL RLMVGVQVTE
160 170 180 190
EQLENIADRT VQEADEDGDG AVSFVEFTKS LEKMDVEQKM SIRILK
Length:196
Mass (Da):22,452
Last modified:January 23, 2007 - v3
Checksum:iF5113D558AFA27DE
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti127 – 1271R → P.
Corresponds to variant rs35641939 [ dbSNP | Ensembl ].
VAR_048664

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146019 mRNA. Translation: AAG14945.1.
AK290253 mRNA. Translation: BAF82942.1.
AK313062 mRNA. Translation: BAG35891.1.
AC130454 Genomic DNA. No translation available.
CH471145 Genomic DNA. Translation: EAW55801.1.
CCDSiCCDS10617.1.
RefSeqiNP_071380.1. NM_022097.3.
UniGeneiHs.178589.

Genome annotation databases

EnsembliENST00000300113; ENSP00000300113; ENSG00000166869.
GeneIDi63928.
KEGGihsa:63928.
UCSCiuc002dmb.2. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF146019 mRNA. Translation: AAG14945.1.
AK290253 mRNA. Translation: BAF82942.1.
AK313062 mRNA. Translation: BAG35891.1.
AC130454 Genomic DNA. No translation available.
CH471145 Genomic DNA. Translation: EAW55801.1.
CCDSiCCDS10617.1.
RefSeqiNP_071380.1. NM_022097.3.
UniGeneiHs.178589.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2BECX-ray2.70A1-196[»]
ProteinModelPortaliO43745.
SMRiO43745. Positions 11-196.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi121996. 5 interactions.
IntActiO43745. 1 interaction.
MINTiMINT-2729920.
STRINGi9606.ENSP00000300113.

PTM databases

iPTMnetiO43745.

Proteomic databases

PaxDbiO43745.
PRIDEiO43745.

Protocols and materials databases

DNASUi63928.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000300113; ENSP00000300113; ENSG00000166869.
GeneIDi63928.
KEGGihsa:63928.
UCSCiuc002dmb.2. human.

Organism-specific databases

CTDi63928.
GeneCardsiCHP2.
HGNCiHGNC:24927. CHP2.
HPAiCAB072806.
neXtProtiNX_O43745.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0034. Eukaryota.
COG5126. LUCA.
GeneTreeiENSGT00760000119179.
HOGENOMiHOG000233019.
HOVERGENiHBG105307.
InParanoidiO43745.
KOiK17611.
OMAiHFRPVDE.
OrthoDBiEOG78M035.
PhylomeDBiO43745.
TreeFamiTF354284.

Miscellaneous databases

EvolutionaryTraceiO43745.
GenomeRNAii63928.
PROiO43745.

Gene expression databases

BgeeiO43745.
GenevisibleiO43745. HS.

Family and domain databases

Gene3Di1.10.238.10. 1 hit.
InterProiIPR011992. EF-hand-dom_pair.
IPR018247. EF_Hand_1_Ca_BS.
IPR002048. EF_hand_dom.
[Graphical view]
PfamiPF13202. EF-hand_5. 1 hit.
PF13499. EF-hand_7. 1 hit.
[Graphical view]
SMARTiSM00054. EFh. 3 hits.
[Graphical view]
SUPFAMiSSF47473. SSF47473. 1 hit.
PROSITEiPS00018. EF_HAND_1. 2 hits.
PS50222. EF_HAND_2. 3 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Large scale identification of human hepatocellular carcinoma-associated antigens by autoantibodies."
    Wang Y., Han K.-J., Pang X.-W., Vaughan H.A., Qu W., Dong X.-Y., Peng J.-R., Zhao H.-T., Rui J.-A., Leng X.-S., Cebon J., Burgess A.W., Chen W.-F.
    J. Immunol. 169:1102-1109(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], TISSUE SPECIFICITY.
    Tissue: Hepatoma.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Caudate nucleus and Colon.
  3. "The finished DNA sequence of human chromosome 12."
    Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C., Lewis L.R.
    , Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R., Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E., Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G., Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H., Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S., Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H., Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q., Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V., Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E., Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K., Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D., Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R., David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E., D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N., Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N., Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R., Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S., LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H., Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P., Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G., Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E., Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S., Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O., Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J., Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A., Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M., Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I., Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A., Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y., Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A., Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F., Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L., Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G., Gibbs R.A.
    Nature 440:346-351(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Expression of calcineurin B homologous protein 2 protects serum deprivation-induced cell death by serum-independent activation of Na+/H+ exchanger."
    Pang T., Wakabayashi S., Shigekawa M.
    J. Biol. Chem. 277:43771-43777(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY, INTERACTION WITH SLC9A1.
  6. "CHP2 activates the calcineurin/nuclear factor of activated T cells signaling pathway and enhances the oncogenic potential of HEK293 cells."
    Li G.D., Zhang X., Li R., Wang Y.D., Wang Y.L., Han K.J., Qian X.P., Yang C.G., Liu P., Wei Q., Chen W.F., Zhang J., Zhang Y.
    J. Biol. Chem. 283:32660-32668(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION AS A CALCINEURIN ACTIVATOR, INTERACTION WITH PPP3CA.
  7. "Nuclear accumulation of calcineurin B homologous protein 2 (CHP2) results in enhanced proliferation of tumor cells."
    Li Q.H., Wang L.H., Lin Y.N., Chang G.Q., Li H.W., Jin W.N., Hu R.H., Pang T.X.
    Genes Cells 16:416-426(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SLC9A1, SUBCELLULAR LOCATION, MUTAGENESIS OF ASP-50; GLY-82; GLU-135; 137-LEU--VAL-148 AND GLU-176.
  8. "Crystallization and preliminary crystallographic analysis of the human calcineurin homologous protein CHP2 bound to the cytoplasmic region of the Na+/H+ exchanger NHE1."
    Ben Ammar Y., Takeda S., Sugawara M., Miyano M., Mori H., Wakabayashi S.
    Acta Crystallogr. F 61:956-958(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PRELIMINARY X-RAY CRYSTALLOGRAPHY OF 1-195 IN COMPLEX WITH SLC9A1.
  9. "Crystal structure of CHP2 complexed with NHE1-cytosolic region and an implication for pH regulation."
    Ammar Y.B., Takeda S., Hisamitsu T., Mori H., Wakabayashi S.
    EMBO J. 25:2315-2325(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS) OF 1-195 IN COMPLEX WITH SLC9A1.

Entry informationi

Entry nameiCHP2_HUMAN
AccessioniPrimary (citable) accession number: O43745
Secondary accession number(s): A8K2I8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 132 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 16
    Human chromosome 16: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.