ID   CYH3_HUMAN              Reviewed;         400 AA.
AC   O43739; A4D2N8;
DT   21-FEB-2001, integrated into UniProtKB/Swiss-Prot.
DT   21-FEB-2001, sequence version 2.
DT   25-JAN-2012, entry version 105.
DE   RecName: Full=Cytohesin-3;
DE   AltName: Full=ARF nucleotide-binding site opener 3;
DE            Short=Protein ARNO3;
DE   AltName: Full=General receptor of phosphoinositides 1;
DE            Short=Grp1;
DE   AltName: Full=PH, SEC7 and coiled-coil domain-containing protein 3;
GN   Name=CYTH3; Synonyms=ARNO3, GRP1, PSCD3;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RX   MEDLINE=98416124; PubMed=9742223;
RA   Venkateswarlu K., Gunn-Moore F., Oatey P.B., Tavare J.M., Cullen P.J.;
RT   "Nerve growth factor- and epidermal growth factor-stimulated
RT   translocation of the ADP-ribosylation factor-exchange factor GRP1 to
RT   the plasma membrane of PC12 cells requires activation of
RT   phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology
RT   domain.";
RL   Biochem. J. 335:139-146(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
RC   TISSUE=Placenta;
RX   MEDLINE=98374282; PubMed=9707577; DOI=10.1073/pnas.95.17.9926;
RA   Franco M., Boretto J., Robineau S., Monier S., Goud B., Chardin P.,
RA   Chavrier P.;
RT   "ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP
RT   ribosylation factor 1, is involved in the control of Golgi structure
RT   and function.";
RL   Proc. Natl. Acad. Sci. U.S.A. 95:9926-9931(1998).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   MEDLINE=22616434; PubMed=12690205; DOI=10.1126/science.1083423;
RA   Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K.,
RA   Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R.,
RA   Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A.,
RA   Kanematsu E., Gentles S., Christopoulos C.C., Choufani S.,
RA   Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z.,
RA   Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C.,
RA   Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J.,
RA   Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F.,
RA   Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F.,
RA   Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H.,
RA   Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G.,
RA   Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P.,
RA   Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J.,
RA   Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F.,
RA   Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B.,
RA   Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H.,
RA   Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W.,
RA   Mural R.J., Adams M.D., Tsui L.-C.;
RT   "Human chromosome 7: DNA sequence and biology.";
RL   Science 300:767-772(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L.,
RA   Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R.,
RA   Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V.,
RA   Hannenhalli S., Turner R., Yooseph S., Lu F., Nusskern D.R.,
RA   Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H.,
RA   Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G.,
RA   Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W.,
RA   Venter J.C.;
RL   Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC   TISSUE=Brain;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA
RT   project: the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
RN   [6]
RP   ALTERNATIVE SPLICING.
RX   MEDLINE=20119275; PubMed=10652308; DOI=10.1074/jbc.275.5.3221;
RA   Ogasawara M., Kim S.C., Adamik R., Togawa A., Ferrans V.J., Takeda K.,
RA   Kirby M., Moss J., Vaughan M.;
RT   "Similarities in function and gene structure of cytohesin-4 and
RT   cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation
RT   factors.";
RL   J. Biol. Chem. 275:3221-3230(2000).
RN   [7]
RP   SUBCELLULAR LOCATION.
RX   PubMed=17398095; DOI=10.1016/j.cub.2007.03.007;
RA   Hofmann I., Thompson A., Sanderson C.M., Munro S.;
RT   "The Arl4 family of small G proteins can recruit the cytohesin Arf6
RT   exchange factors to the plasma membrane.";
RL   Curr. Biol. 17:711-716(2007).
CC   -!- FUNCTION: Promotes guanine-nucleotide exchange on ARF1. Promotes
CC       the activation of ARF through replacement of GDP with GTP.
CC   -!- SUBUNIT: Binds via its PH domain to the inositol head group of
CC       phosphotidylinositol 3,4,5-triphosphate with high affinity.
CC       Interacts with GRASP (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cell membrane.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=O43739-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=O43739-2; Sequence=VSP_006039;
CC   -!- TISSUE SPECIFICITY: Almost absent from liver, thymus and
CC       peripheral blood lymphocytes.
CC   -!- SIMILARITY: Contains 1 PH domain.
CC   -!- SIMILARITY: Contains 1 SEC7 domain.
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DR   EMBL; AJ005197; CAA06434.1; -; mRNA.
DR   EMBL; AJ223957; CAA11686.1; -; mRNA.
DR   EMBL; CH236963; EAL23717.1; -; Genomic_DNA.
DR   EMBL; CH878731; EAW55046.1; -; Genomic_DNA.
DR   EMBL; BC028717; AAH28717.1; -; mRNA.
DR   IPI; IPI00013892; -.
DR   IPI; IPI00216718; -.
DR   RefSeq; NP_004218.1; NM_004227.3.
DR   UniGene; Hs.487479; -.
DR   ProteinModelPortal; O43739; -.
DR   SMR; O43739; 63-397.
DR   IntAct; O43739; 2.
DR   MINT; MINT-1466258; -.
DR   STRING; O43739; -.
DR   PhosphoSite; O43739; -.
DR   PRIDE; O43739; -.
DR   Ensembl; ENST00000396741; ENSP00000379967; ENSG00000008256.
DR   GeneID; 9265; -.
DR   KEGG; hsa:9265; -.
DR   CTD; 9265; -.
DR   GeneCards; GC07M006168; -.
DR   HGNC; HGNC:9504; CYTH3.
DR   HPA; HPA013979; -.
DR   MIM; 605081; gene.
DR   neXtProt; NX_O43739; -.
DR   PharmGKB; PA164718590; -.
DR   GeneTree; ENSGT00560000076562; -.
DR   HOGENOM; HBG382489; -.
DR   HOVERGEN; HBG002647; -.
DR   InParanoid; O43739; -.
DR   OMA; WMKSIRA; -.
DR   OrthoDB; EOG4J1187; -.
DR   PhylomeDB; O43739; -.
DR   Pathway_Interaction_DB; arf6cyclingpathway; Arf6 signaling events.
DR   Pathway_Interaction_DB; pi3kcipathway; Class I PI3K signaling events.
DR   NextBio; 34729; -.
DR   ArrayExpress; O43739; -.
DR   Bgee; O43739; -.
DR   CleanEx; HS_CYTH3; -.
DR   Genevestigator; O43739; -.
DR   GermOnline; ENSG00000008256; Homo sapiens.
DR   GO; GO:0005737; C:cytoplasm; IBA:RefGenome.
DR   GO; GO:0005624; C:membrane fraction; TAS:ProtInc.
DR   GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR   GO; GO:0005545; F:1-phosphatidylinositol binding; TAS:ProtInc.
DR   GO; GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; IBA:RefGenome.
DR   GO; GO:0005220; F:inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity; TAS:ProtInc.
DR   GO; GO:0032012; P:regulation of ARF protein signal transduction; IEA:InterPro.
DR   GO; GO:0030155; P:regulation of cell adhesion; IBA:RefGenome.
DR   GO; GO:0016192; P:vesicle-mediated transport; TAS:ProtInc.
DR   InterPro; IPR011993; PH_type.
DR   InterPro; IPR001849; Pleckstrin_homology.
DR   InterPro; IPR000904; Sec7.
DR   InterPro; IPR023394; SEC7_alpha_orthog.
DR   Gene3D; G3DSA:1.10.1000.11; G3DSA:1.10.1000.11; 1.
DR   Gene3D; G3DSA:2.30.29.30; PH_type; 1.
DR   Pfam; PF00169; PH; 1.
DR   Pfam; PF01369; Sec7; 1.
DR   SMART; SM00233; PH; 1.
DR   SMART; SM00222; Sec7; 1.
DR   SUPFAM; SSF48425; Sec7; 1.
DR   PROSITE; PS50003; PH_DOMAIN; 1.
DR   PROSITE; PS50190; SEC7; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell membrane; Coiled coil; Complete proteome;
KW   Guanine-nucleotide releasing factor; Membrane; Reference proteome.
FT   CHAIN         1    400       Cytohesin-3.
FT                                /FTId=PRO_0000120200.
FT   DOMAIN       77    206       SEC7.
FT   DOMAIN      264    381       PH.
FT   COILED       14     61       Potential.
FT   VAR_SEQ     277    277       Missing (in isoform 2).
FT                                /FTId=VSP_006039.
SQ   SEQUENCE   400 AA;  46349 MW;  87FB917899EE957D CRC64;
     MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE
     SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL
     GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN
     PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY
     ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT
     TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR
     ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK
//