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Reviewed, UniProtKB/Swiss-Prot O43739 (CYH3_HUMAN)

Last modified November 4, 2008. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytohesin-3
Alternative name(s):
    PH, SEC7 and coiled-coil domain-containing protein 3
    ARF nucleotide-binding site opener 3
      Short name=Protein ARNO3
    General receptor of phosphoinositides 1
      Short name=Grp1
Gene names
Name: PSCD3
Synonyms: ARNO3, GRP1
OrganismHomo sapiens (Human)
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

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Protein attributes

Sequence length400 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at transcript level.

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General annotation (Comments)

Function

Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP.

Subunit structure

Binds via its PH domain to the inositol head group of phosphotidylinositol 3,4,5-triphosphate with high affinity. Interacts with GRASP By similarity.

Tissue specificity

Almost absent from liver, thymus and peripheral blood lymphocytes.

Sequence similarities

Contains 1 PH domain.

Contains 1 SEC7 domain.

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Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O43739-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O43739-2)

The sequence of this isoform differs from the canonical sequence as follows:
     277-277: Missing.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 400400Cytohesin-3
PRO_0000120200

Regions

Domain77 – 206130SEC7
Domain264 – 381118PH
Coiled coil14 – 6148 Potential

Natural variations

Alternative sequence2771Missing in isoform 2.
VSP_006039

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Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified February 21, 2001. Version 2.
Checksum: 87FB917899EE957D

FASTA40046,349
        10         20         30         40         50         60 
MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 

        70         80         90        100        110        120 
SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 

       130        140        150        160        170        180 
GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 

       190        200        210        220        230        240 
PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY 

       250        260        270        280        290        300 
ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT 

       310        320        330        340        350        360 
TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR 

       370        380        390        400 
ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK

« Hide

Isoform 2 [UniParc].

Checksum: DAFCAD4131A9C42D
Show »

39946,292

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References

« Hide 'large scale' references
[1]"ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function."
Franco M., Boretto J., Robineau S., Monier S., Goud B., Chardin P., Chavrier P.
Proc. Natl. Acad. Sci. U.S.A. 95:9926-9931(1998) [PubMed: 9707577] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
Tissue: Placenta.
[2]"Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain."
Venkateswarlu K., Gunn-Moore F., Oatey P.B., Tavare J.M., Cullen P.J.
Biochem. J. 335:139-146(1998) [PubMed: 9742223] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Tissue: Brain.
[4]"Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors."
Ogasawara M., Kim S.C., Adamik R., Togawa A., Ferrans V.J., Takeda K., Kirby M., Moss J., Vaughan M.
J. Biol. Chem. 275:3221-3230(2000) [PubMed: 10652308] [Abstract]
Cited for: ALTERNATIVE SPLICING.
+Additional computationally mapped references.

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Cross-references

Sequence databases

AJ223957 mRNA. Translation: CAA11686.1.
AJ005197 mRNA. Translation: CAA06434.1.
BC028717 mRNA. Translation: AAH28717.1.
RefSeqNP_004218.1.
UniGeneHs.487479

3D structure databases

HSSPHSSP built from PDB template 1FHW based on UniProtKB O08967.
SMRO43739. Positions 59-251, 261-388.
ModBaseSearch...

Protein-protein interaction databases

IntActO43739.

PTM databases

PhosphoSiteO43739.

Genome annotation databases

EnsemblENSG00000008256. Homo sapiens. [Contig view]
GeneID9265.
KEGGhsa:9265.

Organism-specific databases

H-InvDBHIX0006463.
HGNCHGNC:9504. PSCD3.
HPAHPA013979.
MIM605081. gene.
PharmGKBPA33851.
GenAtlasSearch...
GeneCardsSearch...

Phylogenomic databases

HOGENOMO43739.
HOVERGENO43739.

Gene expression databases

ArrayExpressO43739.
CleanExHS_PSCD3.
GermOnlineENSG00000008256. Homo sapiens.

Family and domain databases

InterProIPR001849. PH.
IPR011993. PH_type.
IPR000904. Sec7.
[Graphical view]
Gene3DG3DSA:2.30.29.30. PH_type. 1 hit.
PfamPF00169. PH. 1 hit.
PF01369. Sec7. 1 hit.
[Graphical view]
SMARTSM00233. PH. 1 hit.
SM00222. Sec7. 1 hit.
[Graphical view]
PROSITEPS50003. PH_DOMAIN. 1 hit.
PS50190. SEC7. 1 hit.
[Graphical view]
BLOCKSSearch...
ProtoNetSearch...

Other Resources

NextBio34729.
SOURCESearch...

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Entry information

Entry nameCYH3_HUMAN
AccessionPrimary (citable) accession number: O43739
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: February 21, 2001
Last modified: November 4, 2008
This is version 74 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

Human chromosome 7

Human chromosome 7: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Alternative products · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents