O43739 (CYH3_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytohesin-3

Alternative name(s):
ARF nucleotide-binding site opener 3
Short name=Protein ARNO3
General receptor of phosphoinositides 1
Short name=Grp1
PH, SEC7 and coiled-coil domain-containing protein 3

Gene names

Name:	CYTH3
Synonyms:	ARNO3, GRP1, PSCD3

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Promotes guanine-nucleotide exchange on ARF1. Promotes the activation of ARF through replacement of GDP with GTP.
Subunit structure	Binds via its PH domain to the inositol head group of phosphatidylinositol 3,4,5-trisphosphate with high affinity. Interacts with GRASP By similarity.
Subcellular location	Cell membrane Ref.7.
Tissue specificity	Almost absent from liver, thymus and peripheral blood lymphocytes.
Sequence similarities	Contains 1 PH domain. Contains 1 SEC7 domain.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Coiled coil
Molecular function	Guanine-nucleotide releasing factor
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	establishment of epithelial cell polarity Inferred from electronic annotation. Source: Compara positive regulation of cell adhesion Inferred from electronic annotation. Source: Compara regulation of ARF protein signal transduction Inferred from electronic annotation. Source: InterPro regulation of cell adhesion Inferred from Biological aspect of Ancestor. Source: RefGenome vesicle-mediated transport Traceable author statement Ref.2. Source: ProtInc
Cellular_component	cytoplasm Inferred from electronic annotation. Source: Compara plasma membrane Inferred from direct assay Ref.7. Source: UniProtKB ruffle Inferred from electronic annotation. Source: Compara
Molecular_function	1-phosphatidylinositol binding Traceable author statement Ref.1. Source: ProtInc ARF guanyl-nucleotide exchange factor activity Inferred from Biological aspect of Ancestor. Source: RefGenome inositol 1,4,5-trisphosphate-sensitive calcium-release channel activity Traceable author statement Ref.1. Source: ProtInc phosphatidylinositol-3,4,5-trisphosphate binding Inferred from electronic annotation. Source: Compara
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: O43739-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: O43739-2)

The sequence of this isoform differs from the canonical sequence as follows:
     277-277: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Cytohesin-3		PRO_0000120200
Regions
	Domain	77 – 206	130	SEC7
	Domain	264 – 381	118	PH
	Coiled coil	14 – 61	48	Potential
Natural variations
	Alternative sequence	277	1	Missing in isoform 2.		VSP_006039

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified February 21, 2001. Version 2. Checksum: 87FB917899EE957D	FASTA	400	46,349
	10 20 30 40 50 60 MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 70 80 90 100 110 120 SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 130 140 150 160 170 180 GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 190 200 210 220 230 240 PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY 250 260 270 280 290 300 ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGGRVK TWKRRWFILT DNCLYYFEYT 310 320 330 340 350 360 TDKEPRGIIP LENLSIREVE DPRKPNCFEL YNPSHKGQVI KACKTEADGR VVEGNHVVYR 370 380 390 400 ISAPSPEEKE EWMKSIKASI SRDPFYDMLA TRKRRIANKK
	Isoform 2 [UniParc]. Checksum: DAFCAD4131A9C42D	FASTA	399	46,292
	10 20 30 40 50 60 MDEDGGGEGG GVPEDLSLEE REELLDIRRR KKELIDDIER LKYEIAEVMT EIDNLTSVEE 70 80 90 100 110 120 SKTTQRNKQI AMGRKKFNMD PKKGIQFLIE NDLLQSSPED VAQFLYKGEG LNKTVIGDYL 130 140 150 160 170 180 GERDEFNIKV LQAFVELHEF ADLNLVQALR QFLWSFRLPG EAQKIDRMME AFASRYCLCN 190 200 210 220 230 240 PGVFQSTDTC YVLSFAIIML NTSLHNHNVR DKPTAERFIA MNRGINEGGD LPEELLRNLY 250 260 270 280 290 300 ESIKNEPFKI PEDDGNDLTH TFFNPDREGW LLKLGGRVKT WKRRWFILTD NCLYYFEYTT 310 320 330 340 350 360 DKEPRGIIPL ENLSIREVED PRKPNCFELY NPSHKGQVIK ACKTEADGRV VEGNHVVYRI 370 380 390 SAPSPEEKEE WMKSIKASIS RDPFYDMLAT RKRRIANKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Nerve growth factor- and epidermal growth factor-stimulated translocation of the ADP-ribosylation factor-exchange factor GRP1 to the plasma membrane of PC12 cells requires activation of phosphatidylinositol 3-kinase and the GRP1 pleckstrin homology domain." Venkateswarlu K., Gunn-Moore F., Oatey P.B., Tavare J.M., Cullen P.J. Biochem. J. 335:139-146(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
[2]	"ARNO3, a Sec7-domain guanine nucleotide exchange factor for ADP ribosylation factor 1, is involved in the control of Golgi structure and function." Franco M., Boretto J., Robineau S., Monier S., Goud B., Chardin P., Chavrier P. Proc. Natl. Acad. Sci. U.S.A. 95:9926-9931(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Placenta.
[3]	"Human chromosome 7: DNA sequence and biology." Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S. , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C. Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Tissue: Brain.
[6]	"Similarities in function and gene structure of cytohesin-4 and cytohesin-1, guanine nucleotide-exchange proteins for ADP-ribosylation factors." Ogasawara M., Kim S.C., Adamik R., Togawa A., Ferrans V.J., Takeda K., Kirby M., Moss J., Vaughan M. J. Biol. Chem. 275:3221-3230(2000) [PubMed] [Europe PMC] [Abstract] Cited for: ALTERNATIVE SPLICING.
[7]	"The Arl4 family of small G proteins can recruit the cytohesin Arf6 exchange factors to the plasma membrane." Hofmann I., Thompson A., Sanderson C.M., Munro S. Curr. Biol. 17:711-716(2007) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ005197 mRNA. Translation: CAA06434.1. AJ223957 mRNA. Translation: CAA11686.1. CH236963 Genomic DNA. Translation: EAL23717.1. CH878731 Genomic DNA. Translation: EAW55046.1. BC028717 mRNA. Translation: AAH28717.1.
IPI	IPI00013892. IPI00216718.
RefSeq	NP_004218.1. NM_004227.3.
UniGene	Hs.487479.
3D structure databases
ProteinModelPortal	O43739.
ModBase	Search...
Protein-protein interaction databases
IntAct	O43739. 2 interactions.
MINT	MINT-1466258.
STRING	9606.ENSP00000297044.
PTM databases
PhosphoSite	O43739.
Proteomic databases
PaxDb	O43739.
PRIDE	O43739.
Protocols and materials databases
DNASU	9265.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000350796; ENSP00000297044; ENSG00000008256.
GeneID	9265.
KEGG	hsa:9265.
UCSC	uc003spt.3. human.
Organism-specific databases
CTD	9265.
GeneCards	GC07M006168.
HGNC	HGNC:9504. CYTH3.
HPA	HPA013979.
MIM	605081. gene.
neXtProt	NX_O43739.
PharmGKB	PA164718590.
GenAtlas	Search...
Phylogenomic databases
eggNOG	COG5307.
HOGENOM	HOG000253023.
HOVERGEN	HBG002647.
InParanoid	O43739.
OMA	WMKSIRA.
OrthoDB	EOG4J1187.
Enzyme and pathway databases
Pathway_Interaction_DB	arf6cyclingpathway. Arf6 signaling events. pi3kcipathway. Class I PI3K signaling events.
Gene expression databases
ArrayExpress	O43739.
Bgee	O43739.
CleanEx	HS_CYTH3.
Genevestigator	O43739.
GermOnline	ENSG00000008256. Homo sapiens.
Family and domain databases
Gene3D	1.10.1000.11. 1 hit. 2.30.29.30. 1 hit.
InterPro	IPR011993. PH_like_dom. IPR001849. Pleckstrin_homology. IPR000904. Sec7. IPR023394. SEC7_alpha_orthog. [Graphical view]
Pfam	PF00169. PH. 1 hit. PF01369. Sec7. 1 hit. [Graphical view]
SMART	SM00233. PH. 1 hit. SM00222. Sec7. 1 hit. [Graphical view]
SUPFAM	SSF48425. Sec7. 1 hit.
PROSITE	PS50003. PH_DOMAIN. 1 hit. PS50190. SEC7. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	9265.
NextBio	34729.
SOURCE	Search...

Entry information

Entry name

CYH3_HUMAN

Accession

Primary (citable) accession number: O43739
Secondary accession number(s): A4D2N8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 21, 2001
Last sequence update:	February 21, 2001
Last modified:	May 29, 2013
This is version 119 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.