O43692 (PI15_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Peptidase inhibitor 15
Short name=PI-15

Alternative name(s):
25 kDa trypsin inhibitor
Short name=p25TI
Cysteine-rich secretory protein 8
Short name=CRISP-8
SugarCrisp

Gene names

Name:	PI15
Synonyms:	CRISP8, P25TI

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Serine protease inhibitor which displays weak inhibitory activity against trypsin. Ref.4
Subcellular location	Secreted.
Tissue specificity	Weakly expressed. Expressed at low level in prostate, mammary gland, salivary gland and thyroid gland. Ref.1 Ref.5
Post-translational modification	N-glycosylated Probable. Ref.1
Sequence similarities	Belongs to the CRISP family. Contains 1 SCP domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Protease inhibitor
PTM	Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Cellular_component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	peptidase inhibitor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Propeptide	20 – 60	41	Probable		PRO_0000287622
	Chain	61 – 258	198	Peptidase inhibitor 15		PRO_0000287623
Regions
	Domain	71 – 211	141	SCP
Amino acid modifications
	Glycosylation	26	1	N-linked (GlcNAc...) Potential
	Glycosylation	36	1	N-linked (GlcNAc...) Potential
	Glycosylation	124	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	O43692 [UniParc]. Last modified June 1, 1998. Version 1. Checksum: 1915A5831637795F	FASTA	258	29,065
	10 20 30 40 50 60 MIAISAVSSA LLFSLLCEAS TVVLLNSTDS SPPTNNFTDI EAALKAQLDS ADIPKARRKR 70 80 90 100 110 120 YISQNDMIAI LDYHNQVRGK VFPPAANMEY MVWDENLAKS AEAWAATCIW DHGPSYLLRF 130 140 150 160 170 180 LGQNLSVRTG RYRSILQLVK PWYDEVKDYA FPYPQDCNPR CPMRCFGPMC THYTQMVWAT 190 200 210 220 230 240 SNRIGCAIHT CQNMNVWGSV WRRAVYLVCN YAPKGNWIGE APYKVGVPCS SCPPSYGGSC 250 TDNLCFPGVT SNYLYWFK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"cDNA cloning of a novel trypsin inhibitor with similarity to pathogenesis-related proteins, and its frequent expression in human brain cancer cells." Yamakawa T., Miyata S., Ogawa N., Koshikawa N., Yasumitsu H., Kanamori T., Miyazaki K. Biochim. Biophys. Acta 1395:202-208(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, GLYCOSYLATION.
[2]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Endometrial adenocarcinoma.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[4]	"Purification and identification of a novel and four known serine proteinase inhibitors secreted by human glioblastoma cells." Koshikawa N., Nakamura T., Tsuchiya N., Isaji M., Yasumitsu H., Umeda M., Miyazaki K. J. Biochem. 119:334-339(1996) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 61-85, FUNCTION.
[5]	"Cloning and expression of a novel cysteine-rich secreted protein family member expressed in thyroid and pancreatic mesoderm within the chicken embryo." Smith D.M., Collins-Racie L.A., Marigo V.A., Roberts D.J., Davis N.M., Hartmann C., Schweitzer R., LaVallie E.R., Gamer L., McCoy J., Tabin C.J. Mech. Dev. 102:223-226(2001) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D45027 mRNA. Translation: BAA25066.1. CR749657 mRNA. Translation: CAH18451.2. BC074931 mRNA. Translation: AAH74931.1. BC074932 mRNA. Translation: AAH74932.1. BC126290 mRNA. Translation: AAI26291.1. BC126292 mRNA. Translation: AAI26293.1.
IPI	IPI00026303.
RefSeq	NP_056970.1. NM_015886.3.
UniGene	Hs.98558.
3D structure databases
ProteinModelPortal	O43692.
ModBase	Search...
PTM databases
PhosphoSite	O43692.
Proteomic databases
PaxDb	O43692.
PRIDE	O43692.
Protocols and materials databases
DNASU	51050.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000260113; ENSP00000260113; ENSG00000137558. ENST00000523773; ENSP00000428567; ENSG00000137558.
GeneID	51050.
KEGG	hsa:51050.
UCSC	uc003yal.3. human.
Organism-specific databases
CTD	51050.
GeneCards	GC08P075773.
HGNC	HGNC:8946. PI15.
HPA	HPA006593. HPA030057.
MIM	607076. gene.
neXtProt	NX_O43692.
PharmGKB	PA33282.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG331624.
HOGENOM	HOG000111978.
HOVERGEN	HBG079090.
InParanoid	O43692.
OMA	MNVWGAV.
OrthoDB	EOG4X6C8W.
Gene expression databases
Bgee	O43692.
CleanEx	HS_PI15.
Genevestigator	O43692.
Family and domain databases
Gene3D	3.40.33.10. 1 hit.
InterPro	IPR001283. Allrgn_V5/Tpx1. IPR018244. Allrgn_V5/Tpx1_CS. IPR014044. CAP_domain. [Graphical view]
PANTHER	PTHR10334. PTHR10334. 1 hit.
Pfam	PF00188. CAP. 1 hit. [Graphical view]
PRINTS	PR00837. V5TPXLIKE.
SMART	SM00198. SCP. 1 hit. [Graphical view]
SUPFAM	SSF55797. SCP-like_extracellular. 1 hit.
PROSITE	PS01009. CRISP_1. False negative. PS01010. CRISP_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
GenomeRNAi	51050.
NextBio	53608.
SOURCE	Search...

Entry information

Entry name

PI15_HUMAN

Accession

Primary (citable) accession number: O43692
Secondary accession number(s): Q68CY1

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 15, 2007
Last sequence update:	June 1, 1998
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.