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Protein

A-kinase anchor protein 7 isoforms alpha and beta

Gene

AKAP7

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel. The membrane-associated form reduces epithelial sodium channel (ENaC) activity, whereas the free cytoplasmic form may negatively regulate ENaC channel feedback inhibition by intracellular sodium.3 Publications

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

GO - Biological processi

  • action potential Source: BHF-UCL
  • cellular response to cAMP Source: BHF-UCL
  • intracellular signal transduction Source: UniProtKB
  • ion transport Source: ProtInc
  • positive regulation of delayed rectifier potassium channel activity Source: BHF-UCL
  • positive regulation of potassium ion transmembrane transport Source: BHF-UCL
  • protein localization Source: UniProtKB
  • regulation of membrane repolarization Source: BHF-UCL
Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
A-kinase anchor protein 7 isoforms alpha and beta
Short name:
AKAP-7 isoforms alpha and beta
Alternative name(s):
A-kinase anchor protein 18 kDa
Short name:
AKAP 18
Protein kinase A-anchoring protein 7 isoforms alpha/beta
Short name:
PRKA7 isoforms alpha/beta
Gene namesi
Name:AKAP7
Synonyms:AKAP15, AKAP18
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 6

Organism-specific databases

HGNCiHGNC:377. AKAP7.

Subcellular locationi

Isoform Alpha :
Isoform Beta :

GO - Cellular componenti

  • apical plasma membrane Source: UniProtKB
  • intracellular Source: GOC
  • lateral plasma membrane Source: UniProtKB
  • plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi2 – 65GQLCC → AQLAA: Abolishes membrane localization and affects its ability to inhibit ENaC activity in a sodium-dependent manner. 1 Publication

Organism-specific databases

PharmGKBiPA24671.

Polymorphism and mutation databases

BioMutaiEPB41L2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved
Chaini2 – 104103A-kinase anchor protein 7 isoforms alpha and betaPRO_0000064522Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycine1 Publication
Lipidationi5 – 51S-palmitoyl cysteine1 Publication
Lipidationi6 – 61S-palmitoyl cysteine1 Publication

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

PaxDbiO43687.
PRIDEiO43687.

PTM databases

iPTMnetiO43687.
SwissPalmiO43687.

Expressioni

Tissue specificityi

Expressed in brain, heart, lung, pancreas and skeletal muscle.1 Publication

Gene expression databases

BgeeiO43687.
CleanExiHS_AKAP7.
ExpressionAtlasiO43687. baseline and differential.
GenevisibleiO43687. HS.

Interactioni

Subunit structurei

Binds cAMP-dependent protein kinase (PKA). Interacts with PRKCA; only the cytoplasmic form is capable of interacting with PRKCA.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CEP76Q8TAP63EBI-10185182,EBI-742887
PRKAR2BQ6DHZ24EBI-10185182,EBI-10185196
ROPN1Q9HAT03EBI-10185182,EBI-1378139
SPA17Q155063EBI-10185182,EBI-1377865

GO - Molecular functioni

  • protein kinase A binding Source: UniProtKB

Protein-protein interaction databases

BioGridi114851. 17 interactions.
IntActiO43687. 4 interactions.
MINTiMINT-5000796.
STRINGi9606.ENSP00000405252.

Structurei

3D structure databases

ProteinModelPortaliO43687.
SMRiO43687. Positions 40-68.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 1110Required for membrane localization
Regioni18 – 4023Required for apical membrane localizationAdd
BLAST
Regioni52 – 6514RII-bindingAdd
BLAST

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
GeneTreeiENSGT00390000012756.
HOGENOMiHOG000013043.
HOVERGENiHBG050474.
KOiK16524.
OrthoDBiEOG773XG9.

Family and domain databases

InterProiIPR019511. AKAP7_RI-RII-bd_dom.
[Graphical view]
PfamiPF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Additional isoforms seem to exist.

Isoform Beta (identifier: O43687-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGQLCCFPFS RDEGKISELE SSSSAVLQRY SKDIPSWSSG EKNGGEPDDA
60 70 80 90 100
ELVRLSKRLV ENAVLKAVQQ YLEETQNKNK PGEGSSVKTE AADQNGNDNE

NNRK
Length:104
Mass (Da):11,465
Last modified:January 23, 2007 - v4
Checksum:i6F84ED8E032AF954
GO
Isoform Alpha (identifier: O43687-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     18-40: Missing.

Show »
Length:81
Mass (Da):8,969
Checksum:iFEDE53F9E2205548
GO
Isoform Gamma (identifier: Q9P0M2-1) [UniParc]FASTAAdd to basket

The sequence of this isoform can be found in the external entry Q9P0M2.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Length:348
Mass (Da):39,518
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei18 – 4023Missing in isoform Alpha. 2 PublicationsVSP_004101Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047715 mRNA. Translation: AAC39715.1.
AF161075 mRNA. Translation: AAF26676.1.
AK290271 mRNA. Translation: BAF82960.1.
AL136110, AL137063 Genomic DNA. Translation: CAI21507.1.
AL136110, AL137063 Genomic DNA. Translation: CAI21508.1.
AL137063, AL136110 Genomic DNA. Translation: CAI19001.1.
AL137063, AL136110 Genomic DNA. Translation: CAI19002.1.
CH471051 Genomic DNA. Translation: EAW48057.1.
BC016927 mRNA. Translation: AAH16927.1.
BC073847 mRNA. Translation: AAH73847.1.
BC094732 mRNA. Translation: AAH94732.1.
CCDSiCCDS5143.1. [O43687-2]
CCDS5144.1. [O43687-1]
RefSeqiNP_004833.1. NM_004842.3. [O43687-2]
NP_619539.1. NM_138633.2. [O43687-1]
UniGeneiHs.486483.
Hs.732515.

Genome annotation databases

EnsembliENST00000342266; ENSP00000345149; ENSG00000118507. [O43687-2]
ENST00000474850; ENSP00000418208; ENSG00000118507. [O43687-1]
GeneIDi9465.
KEGGihsa:9465.
UCSCiuc003qcm.3. human. [O43687-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047715 mRNA. Translation: AAC39715.1.
AF161075 mRNA. Translation: AAF26676.1.
AK290271 mRNA. Translation: BAF82960.1.
AL136110, AL137063 Genomic DNA. Translation: CAI21507.1.
AL136110, AL137063 Genomic DNA. Translation: CAI21508.1.
AL137063, AL136110 Genomic DNA. Translation: CAI19001.1.
AL137063, AL136110 Genomic DNA. Translation: CAI19002.1.
CH471051 Genomic DNA. Translation: EAW48057.1.
BC016927 mRNA. Translation: AAH16927.1.
BC073847 mRNA. Translation: AAH73847.1.
BC094732 mRNA. Translation: AAH94732.1.
CCDSiCCDS5143.1. [O43687-2]
CCDS5144.1. [O43687-1]
RefSeqiNP_004833.1. NM_004842.3. [O43687-2]
NP_619539.1. NM_138633.2. [O43687-1]
UniGeneiHs.486483.
Hs.732515.

3D structure databases

ProteinModelPortaliO43687.
SMRiO43687. Positions 40-68.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi114851. 17 interactions.
IntActiO43687. 4 interactions.
MINTiMINT-5000796.
STRINGi9606.ENSP00000405252.

PTM databases

iPTMnetiO43687.
SwissPalmiO43687.

Polymorphism and mutation databases

BioMutaiEPB41L2.

Proteomic databases

PaxDbiO43687.
PRIDEiO43687.

Protocols and materials databases

DNASUi9465.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000342266; ENSP00000345149; ENSG00000118507. [O43687-2]
ENST00000474850; ENSP00000418208; ENSG00000118507. [O43687-1]
GeneIDi9465.
KEGGihsa:9465.
UCSCiuc003qcm.3. human. [O43687-1]

Organism-specific databases

CTDi9465.
GeneCardsiAKAP7.
HGNCiHGNC:377. AKAP7.
MIMi604693. gene.
neXtProtiNX_O43687.
PharmGKBiPA24671.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2814. Eukaryota.
ENOG4111FMP. LUCA.
GeneTreeiENSGT00390000012756.
HOGENOMiHOG000013043.
HOVERGENiHBG050474.
KOiK16524.
OrthoDBiEOG773XG9.

Miscellaneous databases

ChiTaRSiAKAP7. human.
GeneWikiiAKAP7.
GenomeRNAii9465.
SOURCEiSearch...

Gene expression databases

BgeeiO43687.
CleanExiHS_AKAP7.
ExpressionAtlasiO43687. baseline and differential.
GenevisibleiO43687. HS.

Family and domain databases

InterProiIPR019511. AKAP7_RI-RII-bd_dom.
[Graphical view]
PfamiPF10470. AKAP7_RIRII_bdg. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A novel lipid-anchored A-kinase anchoring protein facilitates cAMP-responsive membrane events."
    Fraser I.D.C., Tavalin S.J., Lester L.B., Langeberg L.K., Westphal A.M., Dean R.A., Marrion N.V., Scott J.D.
    EMBO J. 17:2261-2272(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-5 AND CYS-6.
    Tissue: Brain.
  2. "Alternative splicing regulates the subcellular localization of A-kinase anchoring protein 18 isoforms."
    Trotter K.W., Fraser I.D.C., Scott G.K., Stutts M.J., Scott J.D., Milgram S.L.
    J. Cell Biol. 147:1481-1492(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), FUNCTION, SUBCELLULAR LOCATION.
    Tissue: Lung.
  3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM BETA).
    Tissue: Colon.
  4. "The DNA sequence and analysis of human chromosome 6."
    Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D.
    , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S.
    Nature 425:805-811(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA).
    Tissue: Adrenal cortex, Hippocampus and Lung.
  7. "The A-kinase anchoring protein 15 regulates feedback inhibition of the epithelial Na+ channel."
    Bengrine A., Li J., Awayda M.S.
    FASEB J. 21:1189-1201(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH PRKCA, MUTAGENESIS OF 2-GLY--CYS-6.
  8. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiAKA7A_HUMAN
AccessioniPrimary (citable) accession number: O43687
Secondary accession number(s): A8K2K6
, Q5TBR9, Q5TBS0, Q9HCZ8, Q9P0G4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: June 8, 2016
This is version 127 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 6
    Human chromosome 6: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.