Reviewed, UniProtKB/Swiss-Prot O43687 (AKA7A_HUMAN)

Names and origin

Protein names

Recommended name:
    A-kinase anchor protein 7 isoforms alpha and beta
Alternative name(s):
    Protein kinase A-anchoring protein 7 isoforms alpha/beta
    A-kinase anchor protein 18

Gene names

Name:	AKAP7
Synonyms:	AKAP18

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	104 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Targets the cAMP-dependent protein kinase (PKA) to the plasma membrane, and permits functional coupling to the L-type calcium channel.
Subunit structure	Binds cAMP-dependent protein kinase (PKA).
Subcellular location	Isoform Alpha: Lateral cell membrane; Lipid-anchor. Note= Targeted predominantly to the lateral membrane. Isoform Beta: Apical cell membrane; Lipid-anchor. Note= Targeted predominantly to the apical membrane.
Tissue specificity	Expressed in brain, heart, lung, pancreas and skeletal muscle.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
PTM	Lipoprotein Myristate Palmitate
Gene Ontology (GO)
Biological process	intracellular signaling cascade Ref.2 Traceable author statement. Source: UniProtKB ion transport Ref.1 Traceable author statement. Source: ProtInc protein localization Ref.1 Inferred by curator. Source: UniProtKB
Cellular component	apical plasma membrane Ref.2 Inferred from direct assay. Source: UniProtKB lateral plasma membrane Ref.2 Inferred from direct assay. Source: UniProtKB
Molecular function	protein kinase A binding Ref.1 Ref.2 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Notes: Additional isoforms seem to exist.

Isoform Beta (identifier: O43687-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Notes: Targeted predominantly to the apical membrane.

Isoform Alpha (identifier: O43687-2)

The sequence of this isoform differs from the canonical sequence as follows:
     18-40: Missing.

Notes: Targeted predominantly to the lateral membrane.

Isoform Gamma (identifier: Q9P0M2-1)

The sequence of this isoform can be found in the external entry Q9P0M2-1.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed
	Chain	2 – 104	103	A-kinase anchor protein 7 isoforms alpha and beta		PRO_0000064522
Regions
	Region	2 – 11	10	Required for membrane localization
	Region	18 – 40	23	Required for apical membrane localization
	Region	52 – 65	14	RII-binding
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine
	Lipidation	5	1	S-palmitoyl cysteine
	Lipidation	6	1	S-palmitoyl cysteine
Natural variations
	Alternative sequence	18 – 40	23	Missing in isoform Alpha.		VSP_004101

Sequences

	Sequence		Length	Mass (Da)
	Isoform Beta [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 6F84ED8E032AF954	FASTA	104	11,465
	10 20 30 40 50 60 MGQLCCFPFS RDEGKISELE SSSSAVLQRY SKDIPSWSSG EKNGGEPDDA ELVRLSKRLV 70 80 90 100 ENAVLKAVQQ YLEETQNKNK PGEGSSVKTE AADQNGNDNE NNRK
	Isoform Alpha [UniParc]. Checksum: FEDE53F9E2205548		81	8,969
	10 20 30 40 50 60 MGQLCCFPFS RDEGKISEKN GGEPDDAELV RLSKRLVENA VLKAVQQYLE ETQNKNKPGE 70 80 GSSVKTEAAD QNGNDNENNR K
	Isoform Gamma. See Q9P0M2.		–	–

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A novel lipid-anchored A-kinase anchoring protein facilitates cAMP-responsive membrane events." Fraser I.D.C., Tavalin S.J., Lester L.B., Langeberg L.K., Westphal A.M., Dean R.A., Marrion N.V., Scott J.D. EMBO J. 17:2261-2272(1998) [PubMed: 9545239] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA), FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, MYRISTOYLATION AT GLY-2, PALMITOYLATION AT CYS-5 AND CYS-6. Tissue: Brain.
[2]	"Alternative splicing regulates the subcellular localization of A-kinase anchoring protein 18 isoforms." Trotter K.W., Fraser I.D.C., Scott G.K., Stutts M.J., Scott J.D., Milgram S.L. J. Cell Biol. 147:1481-1492(1999) [PubMed: 10613906] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM BETA), FUNCTION, SUBCELLULAR LOCATION. Tissue: Lung.
[3]	"The DNA sequence and analysis of human chromosome 6." Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L., Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R., Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D., Andrews T.D. , Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H., Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J., Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V., Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J., Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E., Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J., French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J., Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C., Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A., Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R., Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M., Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R., Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M., Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A., Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L., Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I., Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y., Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E., Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A., Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W., Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M., West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J., Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M., Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I., Rogers J., Beck S. Nature 425:805-811(2003) [PubMed: 14574404] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM ALPHA). Tissue: Adrenal cortex, Hippocampus and Lung.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF047715 mRNA. Translation: AAC39715.1. AF161075 mRNA. Translation: AAF26676.1. AL136110, AL137063 Genomic DNA. Translation: CAI21507.1. AL136110, AL137063 Genomic DNA. Translation: CAI21508.1. AL137063, AL136110 Genomic DNA. Translation: CAI19001.1. AL137063, AL136110 Genomic DNA. Translation: CAI19002.1. BC016927 mRNA. Translation: AAH16927.1. BC073847 mRNA. Translation: AAH73847.1. BC094732 mRNA. Translation: AAH94732.1.
RefSeq	NP_004833.1. NP_619539.1.
UniGene	Hs.486483
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSG00000118507. Homo sapiens. [Contig view]
GeneID	9465.
KEGG	hsa:9465.
Organism-specific databases
HGNC	HGNC:377. AKAP7.
MIM	604693. gene.
PharmGKB	PA24671.
GenAtlas	Search...
GeneCards	Search...
Phylogenomic databases
HOVERGEN	O43687.
Gene expression databases
ArrayExpress	O43687.
CleanEx	HS_AKAP7.
GermOnline	ENSG00000118507. Homo sapiens.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	35464.
SOURCE	Search...

Entry information

Entry name

AKA7A_HUMAN

Accession

Primary (citable) accession number: O43687
Secondary accession number(s): Q5TBR9 , Q5TBS0, Q9HCZ8, Q9P0G4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	January 23, 2007
Last modified:	November 4, 2008
This is version 65 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Relevant documents

Human chromosome 6

Human chromosome 6: entries, gene names and cross-references to MIM

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot