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O43683

- BUB1_HUMAN

UniProt

O43683 - BUB1_HUMAN

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Protein

Mitotic checkpoint serine/threonine-protein kinase BUB1

Gene

BUB1

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis.7 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Enzyme regulationi

Autophosphorylated when the cells enters mitosis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei821 – 8211ATPPROSITE-ProRule annotation
Active sitei917 – 9171Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi793 – 8019ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein kinase activity Source: ProtInc
  3. protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  1. apoptotic process Source: UniProtKB-KW
  2. cell proliferation Source: Ensembl
  3. chromosome segregation Source: UniProtKB-KW
  4. mitotic cell cycle Source: Reactome
  5. mitotic cell cycle checkpoint Source: ProtInc
  6. mitotic nuclear division Source: UniProtKB-KW
  7. mitotic spindle assembly checkpoint Source: ProtInc
  8. positive regulation of intrinsic apoptotic signaling pathway Source: Ensembl
  9. regulation of chromosome segregation Source: UniProtKB
  10. regulation of sister chromatid cohesion Source: UniProtKB
  11. spindle assembly checkpoint Source: UniProtKB
  12. viral process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Apoptosis, Cell cycle, Cell division, Chromosome partition, Host-virus interaction, Mitosis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.1. 2681.
ReactomeiREACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.
SignaLinkiO43683.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic checkpoint serine/threonine-protein kinase BUB1 (EC:2.7.11.1)
Short name:
hBUB1
Alternative name(s):
BUB1A
Gene namesi
Name:BUB1
Synonyms:BUB1L
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 2

Organism-specific databases

HGNCiHGNC:1148. BUB1.

Subcellular locationi

Nucleus. Chromosomecentromerekinetochore
Note: Nuclear in interphase cells. Accumulates gradually during G1 and S phase of the cell cycle, peaks at G2/M, and drops dramatically after mitosis. Localizes to the outer kinetochore. Kinetochore localization is required for normal mitotic timing and checkpoint response to spindle damage and occurs very early in prophase. AURKB, CASC5 and INCENP are required for kinetochore localization (By similarity).By similarity

GO - Cellular componenti

  1. condensed chromosome kinetochore Source: UniProtKB
  2. condensed nuclear chromosome, centromeric region Source: Ensembl
  3. cytoplasm Source: HPA
  4. cytosol Source: Reactome
  5. kinetochore Source: UniProtKB
  6. membrane Source: UniProtKB
  7. nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1061A → D or W: Loss of interaction with CASC5. 1 Publication
Mutagenesisi122 – 1221L → G: Loss of interaction with CASC5. 1 Publication
Mutagenesisi130 – 1301A → S: Partial rescue of the spindle-assembly checkpoint activity. Increased rate of chromosome congression errors. Impaired localization to kinetochores and loss of kinetochore binding of CENPF, SGOL1 and BUBR1 but not of MCAK, MAD1L1 or MAD2L1. 1 Publication
Mutagenesisi535 – 5351K → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-536 and A-537. 1 Publication
Mutagenesisi536 – 5361E → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-535 and A-537. 1 Publication
Mutagenesisi537 – 5371N → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-535 and A-536. 1 Publication
Mutagenesisi609 – 6091T → A: Diminished interaction with PLK1. 1 Publication
Mutagenesisi625 – 6251K → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-626 and A-627. 1 Publication
Mutagenesisi626 – 6261E → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-625 and A-627. 1 Publication
Mutagenesisi627 – 6271N → A: Loss of ubiquitination and CDH1-dependent degradation; when associated with A-625 and A-626. 1 Publication
Mutagenesisi821 – 8211K → A: Loss of activity. 1 Publication

Organism-specific databases

Orphaneti1052. Mosaic variegated aneuploidy syndrome.
PharmGKBiPA81.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10851085Mitotic checkpoint serine/threonine-protein kinase BUB1PRO_0000085671Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei314 – 3141Phosphoserine2 Publications
Modified residuei375 – 3751Phosphoserine1 Publication
Modified residuei563 – 5631Phosphoserine2 Publications
Modified residuei593 – 5931Phosphoserine3 Publications
Modified residuei596 – 5961Phosphoserine6 Publications
Modified residuei609 – 6091Phosphothreonine; by CDK11 Publication
Modified residuei655 – 6551Phosphoserine2 Publications

Post-translational modificationi

Upon spindle-assembly checkpoint activation it is hyperphosphorylated and its kinase activity toward CDC20 is stimulated. Phosphorylation at Thr-609 is required for interaction with PLK1, phosphorylation at this site probably creates a binding site for the POLO-box domain of PLK1, thus enhancing the PLK1-BUB1 interaction.10 Publications
Ubiquitinated and degraded during mitotic exit by APC/C-Cdh1.1 Publication

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO43683.
PaxDbiO43683.
PRIDEiO43683.

PTM databases

PhosphoSiteiO43683.

Expressioni

Tissue specificityi

High expression in testis and thymus, less in colon, spleen, lung and small intestine. Expressed in fetal thymus, bone marrow, heart, liver, spleen and thymus. Expression is associated with cells/tissues with a high mitotic index.

Inductioni

Inhibited by phorbol 12-myristate 13-acetate (PMA).

Gene expression databases

BgeeiO43683.
CleanExiHS_BUB1.
ExpressionAtlasiO43683. baseline and differential.
GenevestigatoriO43683.

Organism-specific databases

HPAiHPA006123.

Interactioni

Subunit structurei

Interacts with BUB3 and CASC5. Interacts (when phosphorylated) with PLK1. The BUB1-BUB3 complex interacts with MAD1L1. Interacts with SV40 Large T antigen; this interaction induces activation of a DNA damage response and promotes p53/TP53 stabilization and phosphorylation (Probable).5 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
P030702EBI-748936,EBI-617698From a different organism.

Protein-protein interaction databases

BioGridi107164. 36 interactions.
DIPiDIP-24206N.
IntActiO43683. 30 interactions.
MINTiMINT-1460788.
STRINGi9606.ENSP00000302530.

Structurei

Secondary structure

1
1085
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi3 – 1412Combined sources
Helixi23 – 3513Combined sources
Helixi43 – 5614Combined sources
Helixi60 – 623Combined sources
Helixi66 – 7611Combined sources
Helixi82 – 909Combined sources
Turni91 – 977Combined sources
Helixi99 – 11113Combined sources
Helixi115 – 12713Combined sources
Helixi133 – 14513Combined sources
Beta strandi736 – 7394Combined sources
Helixi744 – 7529Combined sources
Helixi758 – 7603Combined sources
Beta strandi764 – 7663Combined sources
Beta strandi779 – 7813Combined sources
Beta strandi783 – 79513Combined sources
Beta strandi797 – 8059Combined sources
Beta strandi818 – 8258Combined sources
Helixi828 – 84013Combined sources
Helixi843 – 8486Combined sources
Beta strandi852 – 8576Combined sources
Beta strandi862 – 8665Combined sources
Helixi874 – 8829Combined sources
Helixi891 – 90919Combined sources
Turni910 – 9123Combined sources
Helixi920 – 9223Combined sources
Beta strandi923 – 9253Combined sources
Helixi927 – 9293Combined sources
Beta strandi942 – 9443Combined sources
Helixi953 – 9553Combined sources
Beta strandi960 – 9623Combined sources
Helixi974 – 9774Combined sources
Helixi985 – 100016Combined sources
Beta strandi1006 – 10094Combined sources
Beta strandi1012 – 10154Combined sources
Helixi1025 – 103612Combined sources
Helixi1047 – 106115Combined sources
Turni1062 – 10654Combined sources
Helixi1066 – 107914Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2LAHNMR-A1-150[»]
3E7EX-ray2.31A724-1085[»]
4A1GX-ray2.60A/B/C/D1-150[»]
ProteinModelPortaliO43683.
SMRiO43683. Positions 1-150, 735-1080.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO43683.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini11 – 182172BUB1 N-terminalPROSITE-ProRule annotationAdd
BLAST
Domaini787 – 1085299Protein kinasePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 146146Necessary for kinetochore localizationAdd
BLAST
Regioni99 – 13234Necessary for interaction with CASC5Add
BLAST
Regioni229 – 25628Necessary for interaction with BUB3Add
BLAST
Regioni458 – 47619Essential for loading of BUBR1, MAD1L1 and MAD2L1 to kinetochoresAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 658Nuclear localization signalSequence Analysis
Motifi535 – 5373KEN box 1
Motifi625 – 6273KEN box 2

Domaini

The KEN box is required for its ubiquitination and degradation.1 Publication
BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. BUB1 subfamily.PROSITE-ProRule annotation
Contains 1 BUB1 N-terminal domain.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG317001.
GeneTreeiENSGT00520000055622.
HOGENOMiHOG000231751.
HOVERGENiHBG003709.
InParanoidiO43683.
KOiK02178.
OMAiDPLDVYD.
PhylomeDBiO43683.
TreeFamiTF105455.

Family and domain databases

InterProiIPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR14030. PTHR14030. 1 hit.
PfamiPF08311. Mad3_BUB1_I. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00777. Mad3_BUB1_I. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: O43683-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MDTPENVLQM LEAHMQSYKG NDPLGEWERY IQWVEENFPE NKEYLITLLE
60 70 80 90 100
HLMKEFLDKK KYHNDPRFIS YCLKFAEYNS DLHQFFEFLY NHGIGTLSSP
110 120 130 140 150
LYIAWAGHLE AQGELQHASA VLQRGIQNQA EPREFLQQQY RLFQTRLTET
160 170 180 190 200
HLPAQARTSE PLHNVQVLNQ MITSKSNPGN NMACISKNQG SELSGVISSA
210 220 230 240 250
CDKESNMERR VITISKSEYS VHSSLASKVD VEQVVMYCKE KLIRGESEFS
260 270 280 290 300
FEELRAQKYN QRRKHEQWVN EDRHYMKRKE ANAFEEQLLK QKMDELHKKL
310 320 330 340 350
HQVVETSHED LPASQERSEV NPARMGPSVG SQQELRAPCL PVTYQQTPVN
360 370 380 390 400
MEKNPREAPP VVPPLANAIS AALVSPATSQ SIAPPVPLKA QTVTDSMFAV
410 420 430 440 450
ASKDAGCVNK STHEFKPQSG AEIKEGCETH KVANTSSFHT TPNTSLGMVQ
460 470 480 490 500
ATPSKVQPSP TVHTKEALGF IMNMFQAPTL PDISDDKDEW QSLDQNEDAF
510 520 530 540 550
EAQFQKNVRS SGAWGVNKII SSLSSAFHVF EDGNKENYGL PQPKNKPTGA
560 570 580 590 600
RTFGERSVSR LPSKPKEEVP HAEEFLDDST VWGIRCNKTL APSPKSPGDF
610 620 630 640 650
TSAAQLASTP FHKLPVESVH ILEDKENVVA KQCTQATLDS CEENMVVPSR
660 670 680 690 700
DGKFSPIQEK SPKQALSSHM YSASLLRLSQ PAAGGVLTCE AELGVEACRL
710 720 730 740 750
TDTDAAIAED PPDAIAGLQA EWMQMSSLGT VDAPNFIVGN PWDDKLIFKL
760 770 780 790 800
LSGLSKPVSS YPNTFEWQCK LPAIKPKTEF QLGSKLVYVH HLLGEGAFAQ
810 820 830 840 850
VYEATQGDLN DAKNKQKFVL KVQKPANPWE FYIGTQLMER LKPSMQHMFM
860 870 880 890 900
KFYSAHLFQN GSVLVGELYS YGTLLNAINL YKNTPEKVMP QGLVISFAMR
910 920 930 940 950
MLYMIEQVHD CEIIHGDIKP DNFILGNGFL EQDDEDDLSA GLALIDLGQS
960 970 980 990 1000
IDMKLFPKGT IFTAKCETSG FQCVEMLSNK PWNYQIDYFG VAATVYCMLF
1010 1020 1030 1040 1050
GTYMKVKNEG GECKPEGLFR RLPHLDMWNE FFHVMLNIPD CHHLPSLDLL
1060 1070 1080
RQKLKKVFQQ HYTNKIRALR NRLIVLLLEC KRSRK
Length:1,085
Mass (Da):122,375
Last modified:June 1, 1998 - v1
Checksum:i38AE5E1F88C53BDC
GO
Isoform 2 (identifier: O43683-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     876-932: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.

Show »
Length:1,028
Mass (Da):115,843
Checksum:i9D5F967383751D9C
GO
Isoform 3 (identifier: O43683-3) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     10-29: Missing.

Note: No experimental confirmation available. Gene prediction based on EST data.

Show »
Length:1,065
Mass (Da):120,002
Checksum:iE6BD9A1AA4ADAA71
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti70 – 701S → T in AAC06259. (PubMed:10366450)Curated
Sequence conflicti276 – 2794MKRK → IRHE in AAC39546. (PubMed:9441741)Curated

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti20 – 201G → D.1 Publication
Corresponds to variant rs35890336 [ dbSNP | Ensembl ].
VAR_040400
Natural varianti36 – 361E → D in colorectal cancer. 1 Publication
Corresponds to variant rs1801328 [ dbSNP | Ensembl ].
VAR_008849
Natural varianti259 – 2591Y → C in pancreatic cancer; associated with N-265; failure to rescue the spindle-assembly checkpoint activity as a result of a deficient recruitment of MAD2L1 and BUBR1 to kinetochores; efficient restoration of chromosome congression; reduced binding to BUB3; rescue of the ability of kinetochores to bind SGOL1 and CENPF but not MCAK. 1 Publication
VAR_015687
Natural varianti265 – 2651H → N in pancreatic cancer; associated with C-259; complete rescue of the spindle-assembly checkpoint activity; increased rate of chromosome congression errors. 1 Publication
VAR_015688
Natural varianti492 – 4921S → Y in colorectal cancer. 1 Publication
VAR_008850
Natural varianti534 – 5341N → D.1 Publication
Corresponds to variant rs36109304 [ dbSNP | Ensembl ].
VAR_040401
Natural varianti648 – 6481P → R in colorectal cancer. 1 Publication
VAR_008851

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei10 – 2920Missing in isoform 3. CuratedVSP_054760Add
BLAST
Alternative sequencei876 – 93257Missing in isoform 2. CuratedVSP_054761Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046078 mRNA. Translation: AAC12729.1.
AF043294 mRNA. Translation: AAB97855.2.
AF053305 mRNA. Translation: AAC06259.1.
AF047471 mRNA. Translation: AAC03122.1.
AF139363
, AF139349, AF139350, AF139351, AF139352, AF139353, AF139354, AF139355, AF139356, AF139357, AF139358, AF139359, AF139360, AF139361, AF139362 Genomic DNA. Translation: AAD43675.1.
AC114776 Genomic DNA. Translation: AAY14706.1.
AC226101 Genomic DNA. No translation available.
CH471237 Genomic DNA. Translation: EAW50355.1.
BC028201 mRNA. Translation: AAH28201.1.
AF011387 mRNA. Translation: AAC39546.1.
CCDSiCCDS33273.1. [O43683-1]
CCDS62984.1. [O43683-3]
CCDS62985.1. [O43683-2]
RefSeqiNP_001265546.1. NM_001278617.1. [O43683-2]
NP_004327.1. NM_004336.4. [O43683-1]
UniGeneiHs.469649.

Genome annotation databases

EnsembliENST00000302759; ENSP00000302530; ENSG00000169679. [O43683-1]
ENST00000409311; ENSP00000386701; ENSG00000169679. [O43683-2]
ENST00000535254; ENSP00000441013; ENSG00000169679. [O43683-3]
GeneIDi699.
KEGGihsa:699.
UCSCiuc002tgc.3. human. [O43683-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Web resourcesi

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046078 mRNA. Translation: AAC12729.1 .
AF043294 mRNA. Translation: AAB97855.2 .
AF053305 mRNA. Translation: AAC06259.1 .
AF047471 mRNA. Translation: AAC03122.1 .
AF139363
, AF139349 , AF139350 , AF139351 , AF139352 , AF139353 , AF139354 , AF139355 , AF139356 , AF139357 , AF139358 , AF139359 , AF139360 , AF139361 , AF139362 Genomic DNA. Translation: AAD43675.1 .
AC114776 Genomic DNA. Translation: AAY14706.1 .
AC226101 Genomic DNA. No translation available.
CH471237 Genomic DNA. Translation: EAW50355.1 .
BC028201 mRNA. Translation: AAH28201.1 .
AF011387 mRNA. Translation: AAC39546.1 .
CCDSi CCDS33273.1. [O43683-1 ]
CCDS62984.1. [O43683-3 ]
CCDS62985.1. [O43683-2 ]
RefSeqi NP_001265546.1. NM_001278617.1. [O43683-2 ]
NP_004327.1. NM_004336.4. [O43683-1 ]
UniGenei Hs.469649.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2LAH NMR - A 1-150 [» ]
3E7E X-ray 2.31 A 724-1085 [» ]
4A1G X-ray 2.60 A/B/C/D 1-150 [» ]
ProteinModelPortali O43683.
SMRi O43683. Positions 1-150, 735-1080.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 107164. 36 interactions.
DIPi DIP-24206N.
IntActi O43683. 30 interactions.
MINTi MINT-1460788.
STRINGi 9606.ENSP00000302530.

Chemistry

BindingDBi O43683.
ChEMBLi CHEMBL1772932.

PTM databases

PhosphoSitei O43683.

Proteomic databases

MaxQBi O43683.
PaxDbi O43683.
PRIDEi O43683.

Protocols and materials databases

DNASUi 699.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000302759 ; ENSP00000302530 ; ENSG00000169679 . [O43683-1 ]
ENST00000409311 ; ENSP00000386701 ; ENSG00000169679 . [O43683-2 ]
ENST00000535254 ; ENSP00000441013 ; ENSG00000169679 . [O43683-3 ]
GeneIDi 699.
KEGGi hsa:699.
UCSCi uc002tgc.3. human. [O43683-1 ]

Organism-specific databases

CTDi 699.
GeneCardsi GC02M111395.
HGNCi HGNC:1148. BUB1.
HPAi HPA006123.
MIMi 602452. gene.
neXtProti NX_O43683.
Orphaneti 1052. Mosaic variegated aneuploidy syndrome.
PharmGKBi PA81.
GenAtlasi Search...

Phylogenomic databases

eggNOGi NOG317001.
GeneTreei ENSGT00520000055622.
HOGENOMi HOG000231751.
HOVERGENi HBG003709.
InParanoidi O43683.
KOi K02178.
OMAi DPLDVYD.
PhylomeDBi O43683.
TreeFami TF105455.

Enzyme and pathway databases

BRENDAi 2.7.11.1. 2681.
Reactomei REACT_150425. Resolution of Sister Chromatid Cohesion.
REACT_150471. Separation of Sister Chromatids.
REACT_682. Mitotic Prometaphase.
SignaLinki O43683.

Miscellaneous databases

ChiTaRSi BUB1. human.
EvolutionaryTracei O43683.
GeneWikii BUB1.
GenomeRNAii 699.
NextBioi 2860.
PROi O43683.
SOURCEi Search...

Gene expression databases

Bgeei O43683.
CleanExi HS_BUB1.
ExpressionAtlasi O43683. baseline and differential.
Genevestigatori O43683.

Family and domain databases

InterProi IPR015661. Bub1/Mad3.
IPR011009. Kinase-like_dom.
IPR013212. Mad3_BUB1_I.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR14030. PTHR14030. 1 hit.
Pfami PF08311. Mad3_BUB1_I. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00777. Mad3_BUB1_I. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS51489. BUB1_N. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

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  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), VARIANT TYR-492.
  2. "Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation."
    Ouyang B., Lan Z., Meadows J., Pan H., Fukasawa K., Li W., Dai W.
    Cell Growth Differ. 9:877-885(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  3. "The human homologue of Bub3 is required for kinetochore localization of Bub1 and a Mad3/Bub1-related protein kinase."
    Taylor S.S., Ha E., McKeon F.
    J. Cell Biol. 142:1-11(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
  4. "Phosphorylation of human MAD1 by the BUB1 kinase in vitro."
    Seeley T.W., Wang L., Zhen J.Y.
    Biochem. Biophys. Res. Commun. 257:589-595(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, PHOSPHORYLATION, INTERACTION WITH BUB3 AND MAD1L1, MUTAGENESIS OF LYS-821.
    Tissue: Testis.
  5. "Characterization of MAD2B and other mitotic spindle checkpoint genes."
    Cahill D.P., da Costa L.T., Carson-Walter E.B., Kinzler K.W., Vogelstein B., Lengauer C.
    Genomics 58:181-187(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), VARIANTS ASP-36 AND ARG-648.
  6. "Generation and annotation of the DNA sequences of human chromosomes 2 and 4."
    Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H.
    , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K.
    Nature 434:724-731(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  7. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  8. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Lung.
  9. "Mammalian BUB1 protein kinases: map positions and in vivo expression."
    Pangilinan F., Li Q., Weaver T., Lewis B.C., Dang C.V., Spencer F.
    Genomics 46:379-388(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 276-1085.
  10. "Bub1 is required for kinetochore localization of BubR1, Cenp-E, Cenp-F and Mad2, and chromosome congression."
    Johnson V.L., Scott M.I., Holt S.V., Hussein D., Taylor S.S.
    J. Cell Sci. 117:1577-1589(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  11. "Phosphorylation of Cdc20 by Bub1 provides a catalytic mechanism for APC/C inhibition by the spindle checkpoint."
    Tang Z., Shu H., Oncel D., Chen S., Yu H.
    Mol. Cell 16:387-397(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH BUB3.
  12. "Human Bub1 defines the persistent cohesion site along the mitotic chromosome by affecting Shugoshin localization."
    Kitajima T.S., Hauf S., Ohsugi M., Yamamoto T., Watanabe Y.
    Curr. Biol. 15:353-359(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Phosphorylation- and polo-box-dependent binding of Plk1 to Bub1 is required for the kinetochore localization of Plk1."
    Qi W., Tang Z., Yu H.
    Mol. Biol. Cell 17:3705-3716(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PLK1, PHOSPHORYLATION AT THR-609, MUTAGENESIS OF THR-609.
  14. "Human Blinkin/AF15q14 is required for chromosome alignment and the mitotic checkpoint through direct interaction with Bub1 and BubR1."
    Kiyomitsu T., Obuse C., Yanagida M.
    Dev. Cell 13:663-676(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH CASC5, MUTAGENESIS OF ALA-106 AND LEU-122.
  15. "KEN-box-dependent degradation of the Bub1 spindle checkpoint kinase by the anaphase-promoting complex/cyclosome."
    Qi W., Yu H.
    J. Biol. Chem. 282:3672-3679(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, UBIQUITINATION, DOMAIN KEN BOX, MUTAGENESIS OF LYS-535; GLU-536; ASN-537; LYS-625; GLU-626 AND ASN-627.
  16. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic kidney.
  17. "Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
    Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
    Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  18. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  19. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  20. "Simian virus 40 large T antigen disrupts genome integrity and activates a DNA damage response via Bub1 binding."
    Hein J., Boichuk S., Wu J., Cheng Y., Freire R., Jat P.S., Roberts T.M., Gjoerup O.V.
    J. Virol. 83:117-127(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH SV40 LARGE T ANTIGEN.
  21. "Bub1 regulates chromosome segregation in a kinetochore-independent manner."
    Klebig C., Korinth D., Meraldi P.
    J. Cell Biol. 185:841-858(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF ALA-130, CHARACTERIZATION OF VARIANTS CYS-259 AND ASN-265.
  22. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-314; SER-375; SER-563; SER-596 AND SER-655, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  23. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-563; SER-593 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  24. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-593 AND SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  25. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  26. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-596, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  27. "A double missense variation of the BUB1 gene and a defective mitotic spindle checkpoint in the pancreatic cancer cell line Hs766T."
    Hempen P.M., Kurpad H., Calhoun E.S., Abraham S., Kern S.E.
    Hum. Mutat. 21:445-445(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS PANCREATIC CANCER CYS-259 AND ASN-265.
  28. "Patterns of somatic mutation in human cancer genomes."
    Greenman C., Stephens P., Smith R., Dalgliesh G.L., Hunter C., Bignell G., Davies H., Teague J., Butler A., Stevens C., Edkins S., O'Meara S., Vastrik I., Schmidt E.E., Avis T., Barthorpe S., Bhamra G., Buck G.
    , Choudhury B., Clements J., Cole J., Dicks E., Forbes S., Gray K., Halliday K., Harrison R., Hills K., Hinton J., Jenkinson A., Jones D., Menzies A., Mironenko T., Perry J., Raine K., Richardson D., Shepherd R., Small A., Tofts C., Varian J., Webb T., West S., Widaa S., Yates A., Cahill D.P., Louis D.N., Goldstraw P., Nicholson A.G., Brasseur F., Looijenga L., Weber B.L., Chiew Y.-E., DeFazio A., Greaves M.F., Green A.R., Campbell P., Birney E., Easton D.F., Chenevix-Trench G., Tan M.-H., Khoo S.K., Teh B.T., Yuen S.T., Leung S.Y., Wooster R., Futreal P.A., Stratton M.R.
    Nature 446:153-158(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: VARIANTS [LARGE SCALE ANALYSIS] ASP-20 AND ASP-534.

Entry informationi

Entry nameiBUB1_HUMAN
AccessioniPrimary (citable) accession number: O43683
Secondary accession number(s): E9PC26
, F5GXI5, O43430, O43643, O60626, Q53QE4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: June 1, 1998
Last modified: November 26, 2014
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  7. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3