Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

ATPase ASNA1

Gene

ASNA1

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail-anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity). May be involved in insulin signaling.UniRule annotation2 Publications

Kineticsi

  1. KM=0.22 mM for ATP1 Publication
  1. Vmax=16.6 nmol/min/mg enzyme for ATP1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei74UniRule annotation1
Binding sitei251ATPUniRule annotation1
Binding sitei278ATPUniRule annotation1
Metal bindingi289Zinc; shared with dimeric partnerUniRule annotation1
Metal bindingi292Zinc; shared with dimeric partnerUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 52ATPUniRule annotation8

GO - Molecular functioni

  • arsenite transmembrane transporter activity Source: ProtInc
  • ATPase activity, coupled to movement of substances Source: Reactome
  • ATP binding Source: UniProtKB-HAMAP
  • metal ion binding Source: UniProtKB-KW
  • transporter activity Source: ProtInc

GO - Biological processi

  • IRE1-mediated unfolded protein response Source: Reactome
  • protein insertion into ER membrane Source: UniProtKB-HAMAP
  • transport Source: ProtInc
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Transport

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciZFISH:HS02764-MONOMER.
BRENDAi3.6.3.16. 2681.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.
SABIO-RKO43681.

Protein family/group databases

TCDBi3.A.19.1.1. the tms recognition/insertion complex (trc) family.

Names & Taxonomyi

Protein namesi
Recommended name:
ATPase ASNA1UniRule annotation (EC:3.6.-.-UniRule annotation)
Alternative name(s):
Arsenical pump-driving ATPaseUniRule annotation
Arsenite-stimulated ATPaseUniRule annotation
Transmembrane domain recognition complex 40 kDa ATPase subunit
hARSA-I
hASNA-I
Gene namesi
Name:ASNA1UniRule annotation
Synonyms:ARSA, TRC40
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:752. ASNA1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: ProtInc
  • endoplasmic reticulum membrane Source: Reactome
  • extracellular exosome Source: UniProtKB
  • nucleolus Source: HPA
  • nucleus Source: HPA
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi46G → R: Abolishes ATPase activity, dominantly inhibits the TA protein insertion pathway. 1 Publication1

Organism-specific databases

DisGeNETi439.
OpenTargetsiENSG00000198356.
PharmGKBiPA25051.

Chemistry databases

DrugBankiDB00171. Adenosine triphosphate.

Polymorphism and mutation databases

BioMutaiASNA1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00001522532 – 348ATPase ASNA1Add BLAST347

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO43681.
MaxQBiO43681.
PaxDbiO43681.
PeptideAtlasiO43681.
PRIDEiO43681.

2D gel databases

OGPiO43681.

PTM databases

iPTMnetiO43681.
PhosphoSitePlusiO43681.
SwissPalmiO43681.

Expressioni

Tissue specificityi

Expressed in the epithelial cells of the liver, kidney, and stomach wall, in the adrenal medulla, in the islet cells of the pancreas, in the red pulp of the spleen, and in cardiac and skeletal muscle.1 Publication

Gene expression databases

BgeeiENSG00000198356.
CleanExiHS_ARSA.
HS_ASNA1.
ExpressionAtlasiO43681. baseline and differential.
GenevisibleiO43681. HS.

Organism-specific databases

HPAiHPA045951.
HPA048087.

Interactioni

Subunit structurei

Homodimer (By similarity). Component of a transmembrane domain recognition complex (TRC) (By similarity). Interacts with SERP1 and SEC61B (By similarity). Interacts with WRB.UniRule annotation3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ASPHQ127973EBI-2515857,EBI-2967294
GET4Q7L5D63EBI-2515857,EBI-711823
GPX7Q96SL43EBI-2515857,EBI-749411
HILPDAQ9Y5L23EBI-2515857,EBI-8803836

Protein-protein interaction databases

BioGridi106931. 47 interactors.
IntActiO43681. 17 interactors.
MINTiMINT-4656878.
STRINGi9606.ENSP00000349887.

Structurei

3D structure databases

ProteinModelPortaliO43681.
SMRiO43681.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the arsA ATPase family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2825. Eukaryota.
COG0003. LUCA.
GeneTreeiENSGT00390000003817.
HOGENOMiHOG000197637.
InParanoidiO43681.
KOiK01551.
OMAiYEIDTHN.
OrthoDBiEOG091G0CAR.
PhylomeDBiO43681.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_03112. Asna1_Get3. 1 hit.
InterProiIPR025723. Anion-transp_ATPase-like_dom.
IPR016300. ATPase_ArsA/GET3.
IPR027542. ATPase_ArsA/GET3_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00345. GET3_arsA_TRC40. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O43681-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGVAGWGV EAEEFEDAPD VEPLEPTLSN IIEQRSLKWI FVGGKGGVGK
60 70 80 90 100
TTCSCSLAVQ LSKGRESVLI ISTDPAHNIS DAFDQKFSKV PTKVKGYDNL
110 120 130 140 150
FAMEIDPSLG VAELPDEFFE EDNMLSMGKK MMQEAMSAFP GIDEAMSYAE
160 170 180 190 200
VMRLVKGMNF SVVVFDTAPT GHTLRLLNFP TIVERGLGRL MQIKNQISPF
210 220 230 240 250
ISQMCNMLGL GDMNADQLAS KLEETLPVIR SVSEQFKDPE QTTFICVCIA
260 270 280 290 300
EFLSLYETER LIQELAKCKI DTHNIIVNQL VFPDPEKPCK MCEARHKIQA
310 320 330 340
KYLDQMEDLY EDFHIVKLPL LPHEVRGADK VNTFSALLLE PYKPPSAQ
Length:348
Mass (Da):38,793
Last modified:May 30, 2000 - v2
Checksum:iDA52C4ACC35C7A36
GO

Sequence cautioni

The sequence AAC50731 differs from that shown. Reason: Frameshift at position 19.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113 – 114EL → DV in AAC50731 (PubMed:8884272).Curated2
Sequence conflicti205C → F in AAC03551 (Ref. 1) Curated1
Sequence conflicti303L → P in BAD97083 (Ref. 3) Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_018844332N → S.1 PublicationCorresponds to variant rs8177499dbSNPEnsembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047469 mRNA. Translation: AAC03551.1.
AK291855 mRNA. Translation: BAF84544.1.
AK223363 mRNA. Translation: BAD97083.1.
AY304483 Genomic DNA. Translation: AAP45050.1.
AC018761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84303.1.
BC002651 mRNA. Translation: AAH02651.1.
U60276 mRNA. Translation: AAC50731.1. Frameshift.
CCDSiCCDS32920.1.
RefSeqiNP_004308.2. NM_004317.3.
UniGeneiHs.465985.

Genome annotation databases

EnsembliENST00000357332; ENSP00000349887; ENSG00000198356.
ENST00000591090; ENSP00000466379; ENSG00000198356.
GeneIDi439.
KEGGihsa:439.
UCSCiuc002muv.3. human.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Web resourcesi

NIEHS-SNPs

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF047469 mRNA. Translation: AAC03551.1.
AK291855 mRNA. Translation: BAF84544.1.
AK223363 mRNA. Translation: BAD97083.1.
AY304483 Genomic DNA. Translation: AAP45050.1.
AC018761 Genomic DNA. No translation available.
CH471106 Genomic DNA. Translation: EAW84303.1.
BC002651 mRNA. Translation: AAH02651.1.
U60276 mRNA. Translation: AAC50731.1. Frameshift.
CCDSiCCDS32920.1.
RefSeqiNP_004308.2. NM_004317.3.
UniGeneiHs.465985.

3D structure databases

ProteinModelPortaliO43681.
SMRiO43681.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106931. 47 interactors.
IntActiO43681. 17 interactors.
MINTiMINT-4656878.
STRINGi9606.ENSP00000349887.

Chemistry databases

DrugBankiDB00171. Adenosine triphosphate.

Protein family/group databases

TCDBi3.A.19.1.1. the tms recognition/insertion complex (trc) family.

PTM databases

iPTMnetiO43681.
PhosphoSitePlusiO43681.
SwissPalmiO43681.

Polymorphism and mutation databases

BioMutaiASNA1.

2D gel databases

OGPiO43681.

Proteomic databases

EPDiO43681.
MaxQBiO43681.
PaxDbiO43681.
PeptideAtlasiO43681.
PRIDEiO43681.

Protocols and materials databases

DNASUi439.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000357332; ENSP00000349887; ENSG00000198356.
ENST00000591090; ENSP00000466379; ENSG00000198356.
GeneIDi439.
KEGGihsa:439.
UCSCiuc002muv.3. human.

Organism-specific databases

CTDi439.
DisGeNETi439.
GeneCardsiASNA1.
HGNCiHGNC:752. ASNA1.
HPAiHPA045951.
HPA048087.
MIMi601913. gene.
neXtProtiNX_O43681.
OpenTargetsiENSG00000198356.
PharmGKBiPA25051.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG2825. Eukaryota.
COG0003. LUCA.
GeneTreeiENSGT00390000003817.
HOGENOMiHOG000197637.
InParanoidiO43681.
KOiK01551.
OMAiYEIDTHN.
OrthoDBiEOG091G0CAR.
PhylomeDBiO43681.

Enzyme and pathway databases

BioCyciZFISH:HS02764-MONOMER.
BRENDAi3.6.3.16. 2681.
ReactomeiR-HSA-381038. XBP1(S) activates chaperone genes.
SABIO-RKO43681.

Miscellaneous databases

ChiTaRSiASNA1. human.
GeneWikiiASNA1.
GenomeRNAii439.
PROiO43681.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000198356.
CleanExiHS_ARSA.
HS_ASNA1.
ExpressionAtlasiO43681. baseline and differential.
GenevisibleiO43681. HS.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
HAMAPiMF_03112. Asna1_Get3. 1 hit.
InterProiIPR025723. Anion-transp_ATPase-like_dom.
IPR016300. ATPase_ArsA/GET3.
IPR027542. ATPase_ArsA/GET3_euk.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10803. PTHR10803. 1 hit.
PfamiPF02374. ArsA_ATPase. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00345. GET3_arsA_TRC40. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiASNA_HUMAN
AccessioniPrimary (citable) accession number: O43681
Secondary accession number(s): A6NHP8
, A8K740, Q53FC6, Q92849
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: May 30, 2000
Last modified: November 30, 2016
This is version 157 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.